• Title/Summary/Keyword: Screening procedures

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Production of Transgenic Pig Harboring the Cellulase Digest Gene(CelD) (섬유소 분해효소 유전자가 도입된 형질전환 돼지 생산)

  • 박진기;이연근;민관식;이창현;이향흔;김광식;장원경;김진회;이훈택
    • Korean Journal of Animal Reproduction
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    • v.26 no.2
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    • pp.87-94
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    • 2002
  • This study was performed during the four seasons for the production of transgenic pigs containing the Cellulase Digest Gene. Purebred Landrace gilts and sows approximately 8∼15 months of age (n=126) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality fur zygote collection. Synchronization and superovulation procedures were used that cyclic gilts were synchronized with 20mg altrenogest (ALT) per day for 9 days after PG600 administration followed by superovulation with 1000 IU pregnant mares serum gonadotropin (PMSG) and 750IU human chorionic gonadotrophin (hCG). The cellulase digestion gene for microinjection is rat elasterase promoter (rEl) linked to CelD gene. After hormone treatment, 1,422 embryos were collected from 91 donors and 95.6% (1,359/1,422) embryos were in 1-cell stage which can be visualized the pronuclei for DNA microinjection. A total of 725 DNA microinjected embryos transferred into 35 recipients and produced 65 piglets from 13 litters. Pregnancy rate according to the number of transferred embryos to recipients was higher the group which received 21 to 24 embryos (50.0%) than other groups 20.0% in less and 33.3% in more. A tail tissue was collected from 65 piglets for biopsy. PCR screening was performed on each DNA sample using two separate sets of primers specific for the 5'- and 3'-flanking region of the rEl-CelD gene. Five of the 65 piglets (7.69%) were positive for the transgene. This study provide useful information regarding production of transgenic pig for bioreactor research.

A Requirement Priority Process of Embedded Systems based on the Dependency and Aspect (의존과 관점 기반 임베디드 시스템의 요구사항 우선순위 프로세스)

  • Hwang, Wi-Yong;Kang, Dong-Su;Song, Chee-Yang;Seong, Jae-Seok;Baik, Doo-Kwon
    • The KIPS Transactions:PartD
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    • v.16D no.5
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    • pp.767-790
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    • 2009
  • Setting up a priorityfor an embedded system is greatly significant because a release plan at the early stage of product developments can properly be established through right decision making procedures based on the priorities. For instance, both dependencies among requirements and the aspects of product developers should be considered into the priorities to improve the embedded system. Especially, trade-offs among the requirements, which are quite different depending on H/W and S/W architecture styles they use, should be acknowledged without exception. However, the selection process on the priority has hitherto been fairly systematic in the existing environment where hardware and software are not being considered at once. Therefore, this paper suggests an dependency and aspect-based model and process for the requirements of the priority. For this, the paper analyzes the trade-offs between the requirements depending on the disparate Architecture styles of H/W and S/W, and it also reflects the viewpoints of the developers. For thelast thing, the model and process suggested will be applied to the case of the development of both cell phones and cameras to gain authenticity and reliability. In conclusion, the danger occurring when the release plan is constructed can be minimized by screening the priorities that optimizes the embedded system more explicitly.

The Characteristics and Maintenance of Assemblypersons' Activity Records (국회의원 활동기록의 특성과 관리방향)

  • Han, Eun-Jeong;Yim, Jin-Hee
    • The Korean Journal of Archival Studies
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    • no.21
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    • pp.117-167
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    • 2009
  • Assemblypersons' activities have organizational and individual nature. The activities of assemblypersons and their assistant staffs become a motive for major work performance of Korean legislature, and are also critical public services affecting contemporary political history, history of political parties and history of assemblyperson's hometown. Moreover, their activities are also characterized by work performance in favor of assemblyperson's personal interests to effectively communicate his or her achievements during term of office to electors with a view to winning reelection after the end of 4-year term of office. Assemblyperson's organization produces various types of record in the performance of these works. From the viewpoint of public archive as a product of assemblypersons' public service performance and from their own personal viewpoints, they can create their own identity by arranging their personal records that can demonstrate their own political philosophy, policy orientation, achievements during term of office, and personal features. In addition, their personal records can have much more values and significance in the sense that those records are available to electors to make it possible to highlight their own images in objective and reliable manner. Conclusively, this study underlined that the efforts for assemblypersons and their assistant staffs to keep their records well could be helpful to streamline their internal works and be also helpful for personal interests of assemblypersons. And this study proposed procedures and methods for deducing and screening a list of production archives to meet a viewpoint of streamlined internal works and needs for keeping personal records, ultimately with a view to giving practical helps for these works.

The Impact Factors That Affect Powerlessness of the Elderly (노인의 무력감에 영향을 미치는 요인)

  • Kim, Kwuy-Bun;Cho, Sung Eun;Lee, Yun Jung
    • 한국노년학
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    • v.32 no.2
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    • pp.487-499
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    • 2012
  • The purpose of this study is to find out the degree of powerlessness factors among elderly and by examining the variables(perceived health status, ability of daily living activity, self-esteem, depression, family support, loneliness) which affect such factors. By searching for methods to improve the quality of life for the elderly, efficient nursing procedures can be developed, thereby leading to successful aging. For the study, we purposively collected 300 elderly above the age of 65, residing in the areas of Seoul, Inchon, and Daejeon. 266 samples, survived the data screening, were analyzed by reliability analysis, Spearman correlation, and a multiple regression. As the results, first, the correlated factors of the elderly's powerlessness included depression(r=.597, p<.01), loneliness (r=.423, p<.01), ability of daily living activity(r=.374, p<.01), perceived health status(r=-.304, p<.01), family support(r=-.384, p<.01), and self-esteem(r=-.420, p<.01). Second, factors influencing of powerlessness of them were to show in order of depression(β=.373), loneliness(β=.265), spouse(β=.099), family support(β=.090), monthly allowances(β=.013), these variables accounted for 45.7% of the variance of the elderly's powerlessness. Depression showed to be the most important of the powerlessness factors for the elderly. These findings suggest that nursing researchers need to consider important factors affecting the elderly's powerlessness and nursing interventions focusing on the issues need to be developed.

2020 Clinical Practice Guideline for Percutaneous Transthoracic Needle Biopsy of Pulmonary Lesions: A Consensus Statement and Recommendations of the Korean Society of Thoracic Radiology

  • Soon Ho Yoon;Sang Min Lee;Chul Hwan Park;Jong Hyuk Lee;Hyungjin Kim;Kum Ju Chae;Kwang Nam Jin;Kyung Hee Lee;Jung Im Kim;Jung Hee Hong;Eui Jin Hwang;Heekyung Kim;Young Joo Suh;Samina Park;Young Sik Park;Dong-Wan Kim;Miyoung Choi;Chang Min Park
    • Korean Journal of Radiology
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    • v.22 no.2
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    • pp.263-280
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    • 2021
  • Percutaneous transthoracic needle biopsy (PTNB) is one of the essential diagnostic procedures for pulmonary lesions. Its role is increasing in the era of CT screening for lung cancer and precision medicine. The Korean Society of Thoracic Radiology developed the first evidence-based clinical guideline for PTNB in Korea by adapting pre-existing guidelines. The guideline provides 39 recommendations for the following four main domains of 12 key questions: the indications for PTNB, pre-procedural evaluation, procedural technique of PTNB and its accuracy, and management of post-biopsy complications. We hope that these recommendations can improve the diagnostic accuracy and safety of PTNB in clinical practice and promote standardization of the procedure nationwide.

Study on Legal Position of Aviation Security Subject in Aviation Safety and Security (공항보안요원의 법적 지위에 관한 연구)

  • Hwang, Ho-Won
    • The Korean Journal of Air & Space Law and Policy
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    • v.21 no.2
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    • pp.157-179
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    • 2006
  • According to the Annex 17 to the Convention on International Civil Aviation, an appropriate authority of each contracting state has to define and allocate tasks and coordinate activities between the departments, agencies and other organizations of the State, airport and aircraft operators and other entities concerned with or responsible for the implementation of various aspects of the national civil aviation security programme. The airport has to take leading role in implementing security tasks at airport area because the airport operator is the provider of airport facilities and services to its customer and the security activities belong to its services. So Republic of Korea Government enact the Law, Aviation Safety and Security. The Purpose of this Act is to prevent any unlawful act in airport facilities with international conventions, including the ICAO to provide for standards, procedures and mandatory matters needed to ensure the safety and security of civil aviation. But the Act has some error. So is this paper to review the revision of aviation security regulation and the changes of aviation security responsibilities and task assignment. There is the term "aviation security personnel", who are charged with the task of preventing any act of disrupting the order and safety in airport. But there is no term "security screening personnel" who performs to detect or search for dangerous object, such as weapons or explosives, which may be used for the unlawful obstruction.

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Dental Assistant and Dental Hygienist-comparison with U.S. (치과 보조 인력과 치과위생사-미국의 제도 비교)

  • Youngyuhn Choi
    • Journal of Korean Dental Hygiene Science
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    • v.6 no.2
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    • pp.65-77
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    • 2023
  • Background: The shortage of dental hygienists as assistant is a great concern to dental clinics, while dental hygienists are rather pursuing the role of oral hygiene control and preventive treatments which is the main role for dental hygienists in the United States. The dental hygienist and dental assistant system in the United States can be a reference in these discussions. Methods: Educational requirements for licensure and work areas for dental hygienists and dental assistants were investigated through the information provided by the American Dental Association (ADA), American Dental Hygienists Association, National Board Dental Hygiene Examination (NBDHE), Dental Assistants Association of America (ADAA), and Dental Assistants National Board (DANB). Results: In the United States, each state has different systems, but in general, dental hygienists obtain licenses after completing 2~3 years of associate degree programs in dental hygiene after obtaining basic learning skills, and mainly perform tasks related to patient screening procedures, oral hygiene management and preventive care. Dental assistants can take the license test after completing a training course of 9~11 months to obtain a dental assistant certification. Additional expanded work typically requires passing state qualification tests, completing a training program, obtaining a degree, or gaining clinical experience for a certain period of time, depending on the state Conclusion: The scope of work of dental hygienists designated by the Medical Engineer Act and the Enforcement Decree in Korea includes both the work of dental hygienists and dental assistants in the United States, and if a dental assistant system like the United States is introduced to address the current shortage of dental assistants, institutional supplementation such as adjustment of the scope of work and expansion of the role of dental hygienists in oral hygiene management and prevention work is needed and in-depth discussion is necessary.