• 대한영상의학회지
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• 제84권1호
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• pp.34-50
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• 2023

미국방사선의학회는 효과적인 국가 폐암 검진 시행을 위해 2019년도에 Lung CT Screening Reporting & Data System (이하 Lung-RADS) 1.0보다 폐암의 위양성을 줄이기 위해 개편된 Lung-RADS 1.1을 발표하였고, 2022년 12월에 새로운 Lung-RADS 1.1 개선안 Lung-RADS^Ⓡ 2022를 발표하였다. Lung-RADS^Ⓡ 2022은 Lung-RADS 1.0과 비교했을 때 결절의 크기는 소수점 첫째 자리까지 측정하고, 늑막근처 결절의 크기가 10 mm 미만인 경우까지 범주 2로 하며, 범주 2에서 간유리 결절의 크기 기준을 30 mm로 높이고, 범주 4B와 4X는 매우 의심으로 변경하며, 기도 결절의 위치와 비정형 폐 낭종의 형태와 벽 두께에 따라 범위를 나누었다. 이에 영상의학과 의사들의 개선된 Lung-RADS^Ⓡ 2022에 대한 이해를 돕고자, 이 종설에서는 Lung-RADS^Ⓡ 2022의 장점, 단점, 그리고 향후 개선점에 대해서 기술하고자 한다.

• BMB Reports
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• 제38권5호
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• pp.517-525
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• 2005

Solid phase peptide synthesis method, which was introduced by Merrifield in 1963, has spawned the concept of combinatorial chemistry. In this review, we summarize the present technologies of solid phase peptide synthesis (SPPS) that are related to combinatorial chemistry. The conventional methods of peptide library synthesis on polymer support are parallel synthesis, split and mix synthesis and reagent mixture synthesis. Combining surface chemistry with the recent technology of microelectronic semiconductor fabrication system, the peptide microarray synthesis methods on a planar solid support are developed, which leads to spatially addressable peptide library. There are two kinds of peptide microarray synthesis methodologies: pre-synthesized peptide immobilization onto a glass or membrane substrate and in situ peptide synthesis by a photolithography or the SPOT method. This review also discusses the application of peptide libraries for high-throughput bioassays, for example, peptide ligand screening for antibody or cell signaling, enzyme substrate and inhibitor screening as well as other applications.

• Applied Biological Chemistry
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• 제44권1호
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• pp.7-11
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• 2001

오이 혹은 모조오이를 설치한 소규모 비닐하우스 내에서 procymidone 훈연제를 연소시킨 후 하우스 내 여러 지점의 단위 면적당 약제의 부착량 분포를 조사하였다. 훈연된 procymidone은 오이잎, 유리판과 여과지 모두 거의 대등하게 부착되는 경향을 보여 오이 잎의 대용으로 유리판과 여과지를 사용할 수 있었다. 모조오이를 사용한 실험에서 Procymidone의 부착량은 잎의 수평 투영면적에 정비례하여 수평면에서 가장 많았고, 수직면에서 가장 적었으며, 잎의 이면에는 표면에 비해 5% 이하만이 부착되었다. 높이와 상하 중첩 거리를 달리하여 잎을 설치하였을 때 높이에 따른 농약의 부착량 차이는 거의 없었으며, 상하 중첩된 경우가 상대적으로 약제 부착량이 적었으나 중첩 거리에 따른 잎간의 부착량 차이는 매우 작았다. 모조오이의 수가 일정한 조건하에서 훈연제 처리량이 증가함에 따라 procymidone의 엽면 부착량이 비례적으로 증가하였다. 훈연제 처리량이 일정한 조건하에서 모조오이의 수를 증가시킴에 따라 procymidone의 엽면 부착량은 총 엽면적과 비닐하우스 지면 면적의 합에 반비례 하여, 총 엽면적이 2배와 3배 증가하였을 때 엽면 부착량은 22%와 36%가 감소하였다.

• 대한화장품학회지
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• 제30권4호
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• pp.479-483
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• 2004

MITF는 미백관련 유전자의 대표적인 조절 인자 단백질로서 미백관련 유전자의 E-box와의 결합정도를 단백질 칩을 이용하여 측정하였다. 융합 단백질 형태의 MITF를 유리 칩에 고정시켰고 E-box를 포함하는 DNA oligomer가 결합하는 것을 확인하였다. 형광법, SPR (surface plasmon resonance), SPRi (surface plasmon resonance imaging)방법 중 형광법이 가장 효과적이었으며, DNA 저해제를 사용시 결합이 감소하는 것을 확인하였다. 이 결과 MITF를 이용한 미백원료의 고속스크리닝(HTS)의 가능성을 보여주었다.

• 전기학회논문지
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• 제57권1호
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• pp.137-141
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• 2008

Biochip, which implements bioanalytical process on a tiny surface, is one of candidates for medical diagnosis, drug screening, and molecular sensing. In general, a type of biochip based on microfluidics is composed of microcomponents including microchannel, pump, and valve, which require complicated processes. In this study, gray-scale photolithography(GSPL) was applied to fabricate a biochip with multiple layers. A mould for casting PDMS(polydimethylsiloxane) channel, was fabricated using GSPL. A gray-photomask was prepared by printing gray patterns on a high-quality glossy paper followed by photoreducing by 10:1 onto the photo-film. The formation of multiple layers was studied according to the change of gray level of pattern and the developing time. A biochip composed of a weir(multiple layer structure) and a reaction chamber in a single microchannel was fabricated in a glass plate. Finally, we investigated the application of biochip to antigen-antibody reaction by packing the microbead coated with antibody.

• Parasites, Hosts and Diseases
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• 제47권2호
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• pp.185-187
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• 2009

The prevalence of Enterobius vermicularis infection among preschool children was reported to be low based on a 5-year screening program in Taipei City, Taiwan. The Taipei City government intended to terminate the E. vermicularis screening program among preschool children. Thus, we were entrusted with confirming whether pinworm infections among preschool children in Taipei City had truly declined. From each of 12 administrative districts 2-3 kindergartens were randomly selected for investigation. In total, 4,349 children were examined, of which 2,537 were boys and 1,812 were girls. The cellophane tape adhered to a glass slide was used, and all examinations were done by certified medical technologists. Results indicated that the overall prevalence rate of pinworm infections was 0.62% (27/4,349). Although the infection rate was higher among boys (0.67%, 17/2,537) than in girls (0.55%, 10/1,812), no significant difference was found ($X^2$=0.399, P=0.62). According to the administrative district, the infection rate ranged from no positive cases of E. vermicularis infection in the Xinyi, Zhongzhen, and Wanhua Districts (0%; 0/299, 0/165, and 0/358, respectively), to 0.26% (1/131) in Songshan District, with the highest rate of 1.88% (7/373) in Wenshan District. Because the overall infection rate (0.62%, 27/4,349) in the present study was unchanged compared to that (0.40%, 197/49,541) previously reported in 2005, we propose that regular pinworm screening and treatment programs should be continued in some parts of Taipei City.

• BMB Reports
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• 제37권5호
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• pp.546-551
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• 2004

The increasing pace of development in molecular biology during the last decade has had a direct effect on mass testing and diagnostic applications, including blood screening. We report the model Microarray that has been developed for Hepatitis B virus (HBV) and Hepatitis D virus (HDV) detection. The specific primer pairs of PCR were designed using the Primer Premier 5.00 program according to the conserved regions of HBV and HDV. PCR fragments were purified and cloned into pMD18-T vectors. The recombinant plasmids were extracted from positive clones and the target gene fragments were sequenced. The DNA microarray was prepared by robotically spotting PCR products onto the surface of glass slides. Sequences were aligned, and the results obtained showed that the products of PCR amplification were the required specific gene fragments of HBV, and HDV. Samples were labeled by Restriction Display PCR (RD-PCR). Gene chip hybridizing signals showed that the specificity and sensitivity required for HBV and HDV detection were satisfied. Using PCR amplified products to construct gene chips for the simultaneous clinical diagnosis of HBV and HDV resulted in a quick, simple, and effective method. We conclude that the DNA microarray assay system might be useful as a diagnostic technique in the clinical laboratory. Further applications of RD-PCR for the sample labeling could speed up microarray multi-virus detection.

• KSBB Journal
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• 제15권2호
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• pp.214-218
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• 2000

면역크로마토그래피 진단방법을 이용하는 B형간염 스크리닝 kit를 개발하기 위하여 두가지의 항체를 이용하였다. 표식자항체로 사용된 것은 단세포군항체 anti-HBs이고 포획항체는 goat anti-HBs 인데 포획항체는 니트로셀룰로즈 막에 고정되고 표식자항체는 금 입자에 결합된다. 혈청 검체를 well에 가하면 유리섬유 표면에 건조상태로 침착되어 있던 conjugate가 활성화되어 검체중의 HBsAg와 결합한다. 검체를 가한 지 5분 후 검사결과가 나타나는데 HBsAg와 conjugate가 결합된 복합체가 니트로셀롤로즈 막의 하단부에 붉은 색 선으로 나타난다. 본 kit의 검출한계는 표준 HBs-Ag 용액을 사용하여 시험하였을 때 2 ng/ml이었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7381-7384
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• 2015

This study was conducted to 1206 women who had cervical cancer screening at Chonburi Cancer Hospital. The spilt-sample study aimed to compare the efficacy of abnormal cervical cells detection between liquid-based cytology (LBC) and conventional cytology (CC). The collection of cervical cells was performed by broom and directly smeared on a glass slide for CC then the rest of specimen was prepared for LBC. All slides were evaluated and classified by The Bethesda System. The results of the two cytological tests were compared to the gold standard. The LBC smear significantly decreased inflammatory cell and thick smear on slides. These two techniques were not difference in detection rate of abnormal cytology and had high cytological diagnostic agreement of 95.7%. The histologic diagnosis of cervical tissue was used as the gold standard in 103 cases. Sensitivity, specificity, positive predictive value, negative predictive value, false positive, false negative and accuracy of LBC at ASC-US cut off were 81.4, 75.0, 70.0, 84.9, 25.0, 18.6 and 77.7%, respectively. CC had higher false positive and false negative than LBC. LBC had shown higher sensitivity, specificity, PPV, NPV and accuracy than CC but no statistical significance. In conclusion, LBC method can improve specimen quality, more sensitive, specific and accurate at ASC-US cut off and as effective as CC in detecting cervical epithelial cell abnormalities.

• 한국식물병리학회지
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• 제14권4호
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• pp.325-330
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• 1998

On the basis of the fact that the pycnidiospore of Botryosphaeria dothidea, the causal fungus of apple white rot is a typical water borne spore, a method for quantitative analysis of pycnidiospore dispersal from the warts produced on the diseased apple tree stem was developed. The warts on which cracks developed either on or around them were cut off at the base, and shaked in the water for 4hours at 2$0^{\circ}C$, in which condition the maximum number of spores were released. The volume of shaking solution was calculated as 1 ml per one wart. At the end of shaking, Trio, a household detergent was added to the shaking solution to the concentration of 0.1%, and shaked for additional 10 minutes at 35$^{\circ}C$ to take off the spores attached on the glass ware. One milliliter of the spore suspension thus prepared were passed through transparent membrane filter (pore size : 3.0 ${\mu}{\textrm}{m}$), and the spores attached on the filter were counted under a microscope ($\times$200) after staining them with lactophenol supplemented with aniline blue. The results thus obtained were statistically consistent when at least 30 warts were used simultaneously in single shaking. This method can be applicable in the elucidation of ecology of sporulation and spore dispersal, and also in the screening of the sporulation inhibitor which can be used in the control of the disease by reducing the inoculum density.