• Proceedings of the Korea Society of Environmental Toocicology Conference
• /
• 1996.12a
• /
• pp.64-64
• /
• 1996

Transgenic animal and cell line models which are recently developed in toxicology field combined with molecular biological technique, are powerful tools for studying of mutation in vivo and in vitro, respectively. The Big Blue mutagenesis assay system is one of the most widely used transgenic systems. Especially, for the study of direct acting mutagens, Big Blue cell line is very useful and powerful to evaluate mutagenicity because the mutation frequency and mutationspectrlun showed no distinct differences between cell line and animal. The Big Blue cell lines carry stably integrated copies of lambda shuttle vector containing lac I gene as a mutational target. These lambda shuttle vectors are useful for various mutagenesis related studies in eukaryotic system due to their ability to be exposed mutagen and then transfer a suitable target DNA sequence to it convenient organism for analysis. We tried to assess the mutagenic effect of 4-NQO with Big Blue cell line. After the treatment of 4-NQO, genomic DNA was isolated and lambda shuttle vector was packaged by in Vitro packaging and then these were plated on bacterial host in the presence of X-gal to screen mutation in the lac I. We determined MF as a ratio of blue plaques versus colorless plaques and now undergoing the mutation spectrum of 4-NQO in lac J gene sequence.

• Spatial Information Research
• /
• v.16 no.2
• /
• pp.207-218
• /
• 2008

Providing services in a wireless environment with existing wired-based GIS solutions have many limitations such as slow communication, processing rates and screen size. This study suggested data optimization techniques in a mobile environment to overcome those limitations in four steps. In order to test the methods suggested in the study, experiments are conducted using Gangnam-gu as a test site. An existing GIS engine built in a wired environment was compared with the optimized GIS engine from the study in terms of performance in the same environment. They were also compared and analyzed in terms of response data size, number of requests processed per second, and average time to process a request. The results proved that the proposed engine shows significant improvements in performance compared with the wired GIS.

• Journal of KSNVE
• /
• v.5 no.4
• /
• pp.543-553
• /
• 1995

Modern steam turbine generators are being built as a higher power and larger system, experiencing more frequent starts and stops of operation due to a constant change of power demands. Hence, they are inevitably more vulnerable to various vibrations, and more often exposed to the danger of sudden vibration accidents than ever before. Even under the circumstances, in order to secure the system reliability of steampower plants and there by to supply safely the public electricity, it is important to prevent a sudden vibration accident in one hand and even when it happens, to raise an operating efficiency of the plants throught swift and precise treatments in the other. In this study, an interactive vibration diagnostic system has been developed to make the on-site vibration diagnosis of steam turbine generators possible and convenient, utilizing a note-book PC. For this purpose, at first the principal vibration phenomena, such as various unbalance and unstable vibrations as well as rubbing, misalignment, and shaft crack vibrations, have been systematically classified as grouped parameters of vibration frequencies, amplitudes, phases, rotating speeds at the time of accident, and operating conditions or condition changes. A new complex vibration diagnostic table has been constructed from the causal relations between the characteristic parameters and the principal vibration phenomena. Then, the diagnostic system has been developed to screen and issue the corresponding vibration phenomena by assigning to each user-selected combination of characteristic parameters a unique characteristic vector and comparing this vector with a diagnostic vector of each vibration phenomenon based on the constructed diagnostic table. Moreover, the diagnostic system has a logic whose diagnosis may be performed successfully by inputing only some of the corresponding characteristic parameters without having to input all the parameters. The developed diagnostic system has been applied to perform the diagnosis of several real cases of steam turbine vibration accidents. And the results have been quite satisfactory.

• KIPS Transactions on Software and Data Engineering
• /
• v.3 no.7
• /
• pp.277-284
• /
• 2014

The detection method of curves for the lanes which is powerful for the variation by utilizing the lane variation vector and cardinal spline on the inverse perspective transformation screen images which do not required the camera parameters are suggested in this paper. This method detects the lane area by setting the expected lane area in the s frame and next s+1 frame where the inverse perspective transformation and entire process of the lane filter are adapted, and expects the points of lane location in the next frames with the lane variation vector calculation from the detected lane areas. The scan area is set from the nextly expected lane position and new lane positions are detected within these areas, and the lane variation vectors are renewed with the detected lane position and the lanes are detected with application of cardinal spline for the control points inside the lane areas. The suggested method is a powerful method for curved lane detection, but it was adopted to the linear lanes too. It showed an excellent lane detection speed of about 20ms in processing a frame.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.52 no.2
• /
• pp.129-133
• /
• 2007

To breed virus resistant rice variety, developing an efficient screening method is the most important. Two screening methods such as field screening and tray screening method have been used, but the efficiency of the field screening method is too low because of environment factors and the that of the tray screening method is good but screening capability is limited with only $200{\sim}300$ lines per year. To overcome those problems, mass screening method using screen house was developed. Barely as host plant of vector insect was grown in screen house in winter season. Then viruliferous insects are spread in the first spring of the initiation year and sustain them annually. Screening of virus resistance was tested two times in a year, the first screening was from April to June and the second from July to September. The virus infected rate of each susceptible varieties was increased to 92% for RSV and 100% for RDV from the second year. Also, this method can evaluate as many as $1,500{\sim}2,000$ pedigree lines in one time compared with the tray screening method. The result indicates that the mass screening method using screen house, which combines the advantages of the field and tray screening methods, is proven to be more efficient and reliable.

• Kyungpook Mathematical Journal
• /
• v.60 no.2
• /
• pp.223-238
• /
• 2020

We study a lightlike hypersurface M of an indefinite nearly trans-Sasakian manifold ${\bar{M}}$ with an (ℓ, m)-type connection such that the structure vector field ζ of ${\bar{M}}$ is tangent to M. In particular, we focus on such lightlike hypersurfaces M for which the structure tensor field F is either recurrent or Lie recurrent, or such that M itself is totally umbilical or screen totally umbilical.

• Journal of Biomedical Engineering Research
• /
• v.8 no.2
• /
• pp.171-176
• /
• 1987

Wiener filtering method was applied to the dual energy imaging procedure in digital radiography(D.R.). A linear scanning photodiode arrays with 1024 elements(0.6mm H 1.3mm pixel size) were used to obtain chest images in 0.7 sec. For high energy image acquisition, X-ray tube was set at 140KVp, 100mA with a rare-earth phosphor screen. Low energy image was obtained with X-ray tube setting at 70KVp, 150mA. These measured dual energy images are represented in the vector matrix notation as a linear discrete model including the additive random noise. Then, the object images are restored in the minimum mean square error sense using Wiener filtering method in the transformed domain. These restored high and low energy images are used for computation of the basis image decomposition. Then the basis images are linearly combined to produce bone or tissue selective images. Using this process, we could improve the signal to noise ratio characteristics in the material selective images.

• Journal of the Korean Institute of Telematics and Electronics A
• /
• v.29A no.2
• /
• pp.100-106
• /
• 1992

In this pape, we designed and implemented a hardware accelerator for the generation of bit map font from Hangul outline font description for LBP (Laser Beam Printer) and screen applications Whole system was implemented as a double size PC/AT application board which consists of processing bolck and display block. The processing block has a master processor (MC68000)and two slave processors which are MC56001 and KAFOG chip responsible for the short vector generation. In the display block, TMS34061 was used for monitor display and GP425 was used for LBP print out. The resolution of the monitor is 640$\times$480 and that of LBP is 2385$\times$3390. The current system called KHGB90-B generates about 100 characters per second where each character consists of 32$\times$32 bits

• Fisheries and Aquatic Sciences
• /
• v.6 no.1
• /
• pp.20-26
• /
• 2003

A bacterial strain was isolated from seawater to screen for phytase activities. A colony had the highest activity and was identified as an Escherichia coli strain. Using primers derived from E. coli acid phosphatase appA sequence, we cloned a 1,495 bp DNA fragment connected with the pGEM-T vector. It was over-expressed under lac promoter combined with its native promoter in E. coli $DH5\alpha$. The expression of the phytase gene occurred during late exponential growth and the intracellular phytase production was 16.9 units/ml. The yield of recombinant phytate was 412-fold higher than that of wild type E. coli K13.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• v.1
• /
• pp.133-138
• /
• 2006

Electronic Navigational Charts(ENCs) are official nautical charts which are equivalent to paper charts with supplementary information. Although their main purpose is to be used for the safe navigation of ships, they also contain much information on coasts and seas which may be interesting to ordinary people. However, there is no easy way to access them because of therir specialized data format, access method and visualization. This paper proposes on implementation of SVG for the access and services of ENCs. SVG(Scalable Vector Graphic) makes it possible to make use of Vector graphics for servicing maps in basic internet browsing environment. Implement of SVG for ENC applications by this research is free of special server side GIS mapping system and client side extra technology. The implementation of SVG for ENC Applications can be summarized as follows: Firstly, SVG provides spatial information to possess searching engine to embody SVG map. Secondly, SVG can provide high-quality vector map graphics and interactive facility without special Internet GIS system. It makes it possible to use services with very low cost. Thirdly, SVG information service targeting on maritime transportation can be used as template, so it can be used dynamically any other purpose such as traffic management and vessel monitoring. Many good characteristics of SVG in mapping at computer screen and reusability of SVG document provide new era of visualization of marine geographic information.