• Title/Summary/Keyword: ScpB

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Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria

  • Park, Hye-Jee;Han, Sang-Wook
    • The Plant Pathology Journal
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    • v.33 no.6
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    • pp.602-607
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    • 2017
  • Segregation and condensation protein B (ScpB) is essential for replication and segregation in living organisms. Here, we reported the functions of ScpBXv (ScpB-like protein in Xanthomonas campestris pv. vesicatoria) using phenotypic and proteomic analyses. Growth of $Xcv{\Delta}scpBXv$ (ScpBXv knockout mutant) was reduced under both slow and fast growth conditions in rich medium, but comparable to this of the wild-type in plant-mimic conditions. Interestingly, the mutant was significantly less virulent than the wild-type in tomato, indicating that ScpBXv is involved in virulence. To investigate ScpBXv-associated mechanisms, comparative proteomic analyses were carried out and the abundance of 187 proteins was altered. Among them, diverse transcriptional regulators involved in biofilm formation and virulence were abundant in the wild-type. We further showed that biofilm formation of $Xcv{\Delta}scpBXv$ was reduced. This study provides new insights into the functions of ScpBXv in bacterial replication and biofilm formation, which may contribute to the virulence of Xcv.

Effect of the Addition of Schisandra chinensis Powder on the Physico-chemical Characteristics of Sausage

  • Jin, S.K.;Park, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.12
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    • pp.1753-1761
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    • 2013
  • The individual and interactive effects of Schisandra chinensis powder (SCP) and sodium nitrite additions on color, pH, water holding capacity, residual nitrite, 2-thiobarbituric acid reactive substances (TBARS), volatile basic nitrogen, texture properties, fatty acids, amino acids and sensory evaluation of cooked pork sausages were investigated after 20 d of storage at $4^{\circ}C$. The powders (0, 0.5 and 1.0%) were added to sausages either alone or in combination with nitrite (0 and 100 ppm). SCP added-sausages showed lower $L^*$ (lightness) and W (whiteness) values, and higher $b^*$ (yellowness) values than sausage containing no nitrite, and exhibited the highest $a^*$ values at a 0.5% addition (p<0.05). Residual nitrite and TBARS values were found to be significantly reduced as the addition levels of SCP increased (p<0.05). As the addition of SCP increased, the sausage showed gradually decreased brittleness, cohesiveness, springiness, gumminess and chewiness, while adhesiveness increased. Polyunsaturated fatty acid, n-6 and n-6/n-3 fatty acid ratio concentrations were significantly higher in sausages containing SCP (p<0.05). The addition of SCP to sausage significantly (p<0.05) increased the ammonia content (by 0.5% SCP) and aromatic amino acid concentrations (by 1.0% SCP) (p<0.05). Inclusion of SCP in sausage meat resulted in a significant deterioration in quality characteristics of flavor, springiness, juiciness and overall acceptability (p<0.05). As expected, the observed changes in $a^*$, W, pH, shear force, texture property, TBARS, fatty acid, amino acid and sensory score of sausages, depended on the rate of addition of nitrite (p<0.05). These results suggest that SCP addition is not an effective way of improving the sensory evaluation of sausages, but may beneficially affect TBARS, nitrite scavenging activity, fatty acid and amino acid content in pork sausages.

The Effect of Intensive Weight Shift Training on Seated Postural Balance in Children With Spastic Cerebral Palsy: A Case Study (집중체중 이동훈련이 뇌성마비 아동의 체간조절 능력에 미치는 영향: 사례연구)

  • Ryu, Hyun-Nam;Han, Jin-Tae
    • The Journal of Korean Academy of Sensory Integration
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    • v.17 no.1
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    • pp.9-18
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    • 2019
  • Objective : This study investigated the effect of intensive weight shift training (IWST) on the ability to balance in a sitting posture of children with spastic cerebral palsy (SCP). Methods : One child with spastic diplegia participated in this study for a total of 8 weeks using the ABA experimental design. For the pre-intervention period (A1), general physical therapy (GPT) for children with SCP was performed. The intervention period (B1 and B2), GPT and IWST were conducted for children with SCP, and GPT was conducted again for the post-intervention period (A'1 and A'2). Trunk control capacity was measured using the Korean Trunk Control Measurement Scale (TCMS-K) and Biorescue (RM Ingenierie, France). Results : All TCMS-K variables increased from A1 to B2, and decreased from B2 to A'2. The total area with limit of stability (LOS) increased from A1 to B2, and decreased from B2 to A'2. The ratio of the left/right (Lt/Rt) and anterior/posterior (Ant/Post) LOS area was closer to 1, meaning symmetry, in B2 than in A1. The ratio of the Lt/Rt LOS area decreased further from 1 in A'2 than in B2. The ratio of the Ant/Post LOS area was closer to 1 in A'2 than in B2. Conclusion : IWST had a positive effect on the improvement of balance in the sitting posture of a child with SCP. The results suggest that IWST might help to improve the balance abilities of children with SCP for independent sitting, postural control, and activities of daily living.

Centrifugal Modeling of Sand Compaction Pile (모래다짐말뚝의 원심모델링)

  • Yoo, Nam-Jae;Jeong, Gil-Soo;Kim, Sang-Jin;Chae, Seung-Ho
    • Journal of Industrial Technology
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    • v.21 no.B
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    • pp.187-193
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    • 2001
  • This paper is results of extensive centrifuge model experiments about design factors influencing the bearing capacity and the settlement behaviors of SCP (Sand Compaction Pile). Centrifuge model tests were carried out changing design factors for SCP method such as replacement area ratio (as= 20, 40, 70%), improvement ratio to footing width (W/B = 1, 2, 3), and amount of fines in sand pile (#200 = 5, 10, 15). Therefore, the effects of these design factors on the bearing capacity and the settlement behavior of SCP were investigated and changes of stress concentratio rato due to such an design factors were also investigated. Centrifuge model testing technique for preparing and installing centrifuge model of sand compaction pile, using freezing them, was also developed. As results of centrifuge model tests, more fines in sand compaction pile increases the bearing capacity of SCP. Optimum improvement ratio to footing width was found to be 2. Values of stress concentration ratio was in the ranges of 1.5 - 3.5. The depth of bulging in sand piles was found in the range of 2.0 - 2.5 times of pile diameter.

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Behavior of SCP Improved Ground with Installation of Sheet Pile (Sheet Pile 설치에 따른 SCP개량지반의 거동)

  • Yoo, Nam-Jae;Park, Byung-Soo;Jeong, Gil-Soo
    • Journal of Industrial Technology
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    • v.22 no.B
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    • pp.211-218
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    • 2002
  • The paper is to show the behavior of composit ground which is installed with sheet pile in soft soil improved by sand compaction pile. The results of load-settlement relationship, earth pressure, stress concentration characteristics, and final water content were obtained by centrifuge model test. Two cases of tests, installation of sheet pile on the corner and both side of the loading plate for the improved SCP ground which was designed twice of the footing width, were performed for the tests under the vertical and horizontal loading and both side of corner. Finite element program(CRISP) for sand compaction pile using elasto-plastic model and numerical analysis for soft soil using modified cam-clay constitutive equation were compared and analized with the results of model tests. The result of analysis show the increased bearing capacity of soil after, SCP and sheet pile was installed.

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A Simplified Approach for Predicting Springback in U-Draw Bending of Sheet Metals (용접 판재의 U 드로오 벤딩에서 스프링백 예측을 위한 이론적 단순화)

  • Chang S. H;Seo D. G.
    • Transactions of Materials Processing
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    • v.13 no.8
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    • pp.678-688
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    • 2004
  • The U-draw bending operation is known as a representative test method for springback evaluation of sheet metals since the sheet in U-draw bending operation undergoes stretching, bending and unbending deformations occurred at the stamping process. In this study, a simplified approach was proposed for predicting springback and side-wall curls of tailor-welded blank in U-draw bending operations, using moment-curvature relationships derived for sheets undergoing stretching, bending and unbending deformation. Two different welded strips were adopted to compare the effects of weld-line locations on the springback. One (type A) was welded along the centerline of the strip-width and the other (type B) was welded along the centerline of the strip-length. To investigate the effect of different thickness combination on the springback, the tailor-welded strips were joined by the laser welding process and consisted of three types of thickness combinations of sheets, SCP1 0.8t * SCP1 1.2t, SCP1 0.8t * SCP1 1.6t and SCP1 0.8t * TRIP 1.0t. Some calculated results by the simplified formula were compared with experimental results.

Characteristics of Bearing Capacity and Stress Concentration of Clay Ground Improved with Sand Compaction Piles (SCP 보강 점성토 지반의 지지력 및 응력분담특성)

  • Yoo Nam-Jae;Park Byung-Soo;Jeong Gil-Soo;Koh Kyung-Hwan;Kim Ji-Sung
    • Journal of the Korean Geotechnical Society
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    • v.21 no.1
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    • pp.81-91
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    • 2005
  • This paper is the results of experimental and numerical works on the investigating design factors influencing the bearing capacity, the ratio of stress concentration, and the failure mechanism of the clay ground improved with sand compaction piles (SCP). In order to find the behavior of the clay ground improved with SCP, extensive centrifuge model experiments were carried out for each of the SCP replacement ratio of 20, 40, and $70\%$, the non-plastic fine contents in sand of 5, 10, and $15\%$, and the ratio of the improved width to the loaded area (W/B) of 1, 2, and 3. The commertially available software of FEM, CRISP, was used to analyze test results by performing numerical estimations. In these numerical analyses the sand compaction piles and the clay ground were simulated as a linear elastic and plastic constitutive model and the modified Cam-clay model, based on Critical State Soil Mechanics, respectively.

A study of the determination of off-set position for Nd:YAC laser welding between SCP steel sheet and STS304 sheet (Nd:YAG 레이저빔을 이용한 SCP 강판과 STS304강판 용접시 오프셋(off-set) 위치 결정에 관한 연구)

  • Yoon B. S.;Kim T. H.;Park G. Y.;Lee G. D.
    • Laser Solutions
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    • v.7 no.2
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    • pp.1-10
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    • 2004
  • This work was attempted to join SCP sheet and STS304 sheet by using Nd:YAC laser beam. SCP sheet has good formability and low cost, while STS304 has excellent corrosion resistance and mechanical properties in high temp. In this experiment, butt joint type was used to develop the tailored blank welding for dissimilar steel. Sheets which have different thermal properties. Computer simulation was conducted to obtain the off-set position for efficient welding by considering laser power, scanning speed, focal length and basic properties. The result showed that the optimum thermal distribution was obtained when the laser beam was irradiated at $0.05{\sim}0.1$ mm off-set toward the SCP sheet side. The experiment was conducted based on the result of computer simulation to show the same optimum conditions. Optimum conditions were 3KW in laser beam power, 6m/min in scanning speed, -0.5mm in focal position, 0.1mm off-set toward SCP. Microhardness test, tensile test, bulge test, optical microscopy, EDS, and XRD were performed to observe the microstructure around fusion zone and to evaluate the mechanical properties of optimum conditions, The weld zone had high microhardness values by the formation of the martensitic structure. Tensile test measured the strength of welded region by vertical to strain direction and the elongation of welded region by parallel to strain direction. Bulge test showed $52\%$ formability of the original materials. Bead shape, grain size, and martensitic structure were observed by the optical microscopy in the weld zone. Detailed results of EDS, XRD confirmed that the welded region was connected of martensitic structure.

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Stress Concentration Characteristics of Soft Ground Treated by Sand Compaction Pile (모래다짐말뚝으로 개량된 연약지반의 응력분담특성)

  • Yoo, Nam-Jae;Park, Byung-Soo;Jeong, Gil-Soo;Kim, Sang-Jin
    • Journal of Industrial Technology
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    • v.22 no.A
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    • pp.145-151
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    • 2002
  • This paper is results of extensive centrifuge model experiments about design factors influencing the bearing capacity and the settlement behaviors of SCP (Sand Compaction Pile). Centrifuge model tests were carried out changing design factors for SCP method such as replacement area ratio (as= 20, 40, 70%), Improvement ratio to footing width (W/B = 1, 2, 3), and amount of fines m sand pile (#200 = 5, 10, 15). Therefore, the effects of these design factors on the bearing capacity and the settlement behavior of SCP were investigated and changes of stress concentratio rato due to such an design factors were also investigated. Centrifuge model testing technique for preparing and installing centrifuge model of sand compaction pile, using freezing them, was also developed. As results of centrifuge model tests, more fines in sand compaction pile increases the bearing capacity of SCP. Optimum improvement ratio to footing width was found to be 2. Values of stress concentration ratio was in the ranges of 1.5 - 3.5. The depth of bulging in sand plies was found in the range of 2.0 - 2.5 times of pile diameter.

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Preparation of Nanocrystalline ZnO Ultrafine Powder Using Ultrasonic Spraying Combustion Method (초음파분무 연소법에 의한 나노결정 ZnO 초미분체 제조)

  • Kim, Kwang-Su;Hwang, Du-Sun;Ku, Suk-Kyeon;Lee, Kang;Jeon, Chi-Jung;Lee, Eun-Gu;Kim, Sun-Jae
    • Korean Journal of Materials Research
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    • v.12 no.10
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    • pp.784-790
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    • 2002
  • For mass product of nanocrystalline ZnO ultrafine powders, self-sustaining combustion process(SCP) and ultrasonic spray combustion method(USCM) were applied at the same time. Ultrasonic spray gun was attached on top of the vertical type furnace. The droplet was sprayed into reaction zone of the furnace to form SCP which produces spherical shape with soft agglomerate crystalline ZnO particles. To characterize formed particles, fuel and oxidizing agent for SCP were used glycine and zinc nitrate or zinc hydroxide. Respectively, with changing combustion temperature and mixture ratio of oxidizing agent and fuel, the best ultrasonic spray conditions were obtained. To observe ultrasonic spray effect, two types of powder synthesis processes were compared. One was directly sprayed into furnace from the precursor solution (Type A), the other directly was heated on the hot plate without using spray gun (Type B). Powder obtained by type A was porous sponge shape with heavy agglomeration, but powder obtained using type B was finer primary particle size, spherical shape with weak agglomeration and bigger value of specific surface area. 9/ This can be due to much lower reaction temperature of type B at ignition time than type A. Synthesized nanocrystalline ZnO powders at the best ultrasonic spray conditions have primary particle size in range 20~30nm and specific surface area is about 20m$^2$/g.