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Effects of Keratinase on Performance, Nutrient Utilization, Intestinal Morphology, Intestinal Ecology and Inflammatory Response of Weaned Piglets Fed Diets with Different Levels of Crude Protein

  • Wang, D.;Piao, X.S.;Zeng, Z.K.;Lu, T.;Zhang, Q.;Li, P.F.;Xue, L.F.;Kim, S.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1718-1728
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    • 2011
  • Two experiments were conducted to investigate the in vitro ability of keratinase to hydrolyze soybean glycinin and ${\beta}$-conglycinin and to evaluate the in vivo effects of keratinase when included in corn-soybean diets with different levels of crude protein and fed to nursery pigs. In experiment 1, a saturated keratinase solution (1 ml) was added to two blank controls of either glycinin or ${\beta}$-conglycinin resulting in the hydrolysis of 94.74% glycinin and 88.89% ${\beta}$-conglycinin. In experiment 2, 190 pigs (8.3${\pm}$0.63 kg BW) were allotted to one of four treatments in a 2${\times}$2 factorial arrangement on the basis of body weight, and sex was balanced among the pens. The effects of crude protein (19 vs. 22%) and keratinase (0 vs. 0.05%) were studied. Each treatment was applied to six pens with seven (two pens) or eight pigs per pen. Pigs were fed the experimental diets for 21 d. Weight gain and feed conversion ratio were improved (p<0.05) with keratinase supplementation while feed intake was reduced (p<0.05). Keratinase supplementation increased (p<0.05) the apparent total tract digestibility of dry matter, energy, crude protein and phosphorus. Keratinase supplementation also increased n-butyric acid in the cecum and colon, lactobacilli and total anaerobe counts in the colon as well as the ratio of villus height to crypt depth in the ileum. Additionally, fecal score, ammonia nitrogen and branch chain volatile fatty acids in the colon, E. coli and total aerobe counts in the colon, crypt depth in the jejunum and ileum as well as serum interleukin-1 and interleukin-6 concentrations were also decreased (p<0.05) by keratinase supplementation. A reduction in dietary crude protein decreased (p<0.05) colon ammonia nitrogen concentration and cecal propionic acid and branch chain volatile fatty acid concentrations. In addition, cecal E. coli counts, colon total anaerobe counts, ileal crypt depth, and serum interleukin-1 and interleukin-6 concentrations were also decreased (p<0.05) with the reduction of dietary crude protein. With the exception of fecal scores, there were no significant interactions between crude protein and keratinase. This study provides evidence that dietary keratinase supplementation improved nursery pig performance by improving intestinal morphology and ecology, thus improving nutrient digestibility and alleviating the inflammatory response.

Effect of Untreated Depression in Adolescence on the Suicide Risk and Attempt in Male Young Adults (청소년기 치료받지 못한 우울증이 젊은 성인 남성의 자살 위험성 및 자살 시도에 미치는 영향)

  • Yang, Chan-Mo;Lee, Sang-Yeol
    • Korean Journal of Psychosomatic Medicine
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    • v.28 no.1
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    • pp.29-35
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    • 2020
  • Objectives : Evidence regarding the association between untreated depression in adolescence and suicidal risk in male young adults is scarce. We aimed to assess the effect of untreated illness during adolescence on the suicidal risk and attempt after that first episode. Methods : As part of a cross-sectional study, between May 2017 and April 2018, a total of 260 patients with currently unipolar or bipolar depression were included in the final analysis. Multiple linear and logistic regression analysis were performed to evaluate the association between untreated mood disorder in adolescence and its effect on the suicidal risk and attempt. Results : In total 260 patients, 189 were classified as untreated group. The proportion of suicide attempts, total depression score, suicidal risk and number of suicide attempts were significantly higher in the untreated group. The most predictive factors of suicide attempts were history of untreated depression [Adjusted Odds Ratio (AOR)=4.19, 95% Confidence Interval (CI)=2.25-7.81, p<0.001] and diagnosis of bipolar depression (AOR=2.60, 95% CI=1.52-4.46, p<0.001). Conclusions : Although the untreated depression suggests higher rates of suicidality, a significant proportion (86.7%) of adolescent depression in this study did not receive psychiatric treatment. Future research should be needed to find better ways to decrease barriers in using mental health treatment and its contribution to reduction and prevention of adverse outcome.

A study on the needs for the cool motorcycle helmet and the factors affecting the purchasing intention (오토바이용 쿨 헬멧의 필요성과 구매요인에 관한 조사연구)

  • Seong, Deokhyun;Kim, Jongsoo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.12
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    • pp.8386-8393
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    • 2015
  • This study is to investigate the needs for the cool motorcycle helmet and the factors affecting the purchasing intention of cool helmet. The convenient sampling from motorcycle clubs were conducted during 5/20/2014~5/28/2014 on Google DAX. As a result, 197 participants were surveyed. The main reasons of sensing unpleasant feeling wearing helmet were identified as 'hot and uncomfortable feeling' and 'it ruins hair style'. The mean score expressed in terms of five point scale showed that the needs for cool helmet was 3.9 and the purchasing intention of cool helmet was 3.6, respectively. The significant factors influencing the purchasing intention of cool helmet were identified as 'the needs for cool helmet' (odds ratio=6.52) and 'extra paying intention for cool helmet' (odds ratio=1.77). The main reasons of sensing unpleasant feeling wearing helmet were similar to the previous research. However, we can expect that the initial target market for cool helmet would be the low end (less than \40,000 of extra paying intention) or the high end (more than \100,000 of extra paying intention). The main contribution of this study is dealing with the topics related to the purchasing intention of the cool helmet.

Association of depression with chewing problems in Koreans : A cross-sectional study using the Korea National Health and Nutrition Examination Survey 2016 (한국인에서 씹기 불편감과 우울증의 연관성: 2016 국민건강영양조사를 이용한 단면 연구)

  • Yang, Chan Mo;Baek, Ju Won
    • Journal of Dental Rehabilitation and Applied Science
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    • v.36 no.1
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    • pp.12-20
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    • 2020
  • Purpose: This study was designed to analyze the relationship between the presence and severity of depression and chewing problems (CPs) in a representative sample of the general population. Materials and Methods: Health surveys and examinations were conducted on a nationally representative sample (n = 8150) of Korean was conducted. CPs was determined by a simple survey response concerning "Do you feel uncomfortable about chewing your food because of problems with your mouth such as teeth, dentures and gums?" Depression was defined as individuals with a total score ≥ 10 on the Patient Health Questionnaire (PHQ)-9 survey. Data regarding demographics, socioeconomic history and comorbid health conditions were used to analyze adjusted logistic regression models. Results: In the Korean population, the prevalence of depression was significantly greater in individuals with CP (17.2%) than in those without CP (10.2%). On multivariate logistic regression analysis, the presence of depression was significantly associated with CPs (adjusted odd ratio [aOR]: 1.90, P < 0.001). The risk of CPs increased with increasing severity of depression as follows: severe depression (OR: 2.62, P < 0.001), moderately severe depression (OR: 2.19, P < 0.001). Conclusion: The presence of depression was significantly associated with CPs, especially in severely depressed individuals. Depression screening should be considered in treating CP patients.

The Quality Characteristics of Steamed Egg According to the Ratio of Egg Yolk and Egg White (난황과 난백의 비율을 달리한 달걀찜의 품질특성)

  • Song, Min-Kyung;Kim, Dae-Hyeon;Yoon, Hye Hyun
    • Culinary science and hospitality research
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    • v.20 no.1
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    • pp.65-75
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    • 2014
  • This study prepared steamed eggs at various ratios of yolk and white in packaged liquid eggs to investigate the quality characteristics of steamed eggs through sensory tests and analyses of water content, color, pH and texture profiles. The results are expected to be used in developing steamed egg products and preparing processed egg dishes. Moisture contents of steamed egg samples increased with increased percentage of egg white. Hunter color values of L(lightness) also increased with increased percentage of egg white, while a(redness) and b(yellowness) decreased. Moreover, pH increased with increased percentage of egg white. Texture profile analyses in hardness, springiness, chewiness, gumminess, and cohesiveness decreased significantly with increased percentage of white, but adhesiveness was not significantly different. As the results of sensory test, yellowness increased with increased percentage of yolk. Roasted nutty smell was highest in the steamed egg sample prepared with 250g yolk and 200g white, and fishy smell increased and boiled egg smell decreased with increased percentage of white. Roasted nutty taste and fishy taste increased with increased percentage of white. In contrast with brittleness, hardness, springiness, and chewiness decreased with increased percentage of egg white. The acceptance score was high in the steamed egg samples prepared with a ratio of egg yolk 250/egg white 200 or egg yolk 150/egg white 300.

Quality Characteristics and Antioxidant Activity of Chrysanthemum indicum L., Chrysanthemum boreale M. and Chrysanthemum zawadskii K. Powdered Teas (감국, 산국 및 구절초꽃 분말 차의 항산화활성과 품질특성)

  • Lee, Sang-Hoon;Hwang, In-Guk;Nho, Jin-Woo;Chang, Young-Deug;Lee, Chul-Hee;Woo, Koan-Sik;Jeong, Heon-Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.7
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    • pp.824-831
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    • 2009
  • In order to investigate the quality characteristics of powdered teas using Compositae species flower, the samples of Chrysanthemum indicum L. (CI), Chrysanthemum boreale Makino (CB), and Chrysanthemum zawadskii var. latilobum (Maxim.) Kitam (CZ) were extracted with hot water and concentrated, and then mixed with different forming agents of dextrin (D) and maltodextrin (MD). The mixing ratio of D and MD was ranged from 9:1 (DMD91) to 8:2 (DMD82) or D and MD only. The solubility of the powdered tea was higher in added dextrin. The highest total polyphenol and flavonoid content of the powdered tea were 6.75 and 3.24 mg/g at CBDMD91, respectively. Total antioxidant activities of C. indicum, C. boreale, and C. zawadskii powdered tea ranged at $2.51{\sim}2.63$, $4.37{\sim}4.50$, and $3.44{\sim}3.55\;mg$ AA eq/g, respectively. In sensory evaluation, the C. indicum and C. zawadskii powdered teas obtained higher sensory score of all evaluation items than C. boreale powdered tea. The optimal mixing ratio at 36% of D and 4% of MD in forming agent was selected to enhance the sensory characteristics and antioxidant activities of powdered tea.

A Evaluation Study on Nutrient Intake Status and Diet Quality of Middle and Old Aged Vegetarian Women in Korea (채식을 하는 중$\cdot$노년기 여성의 영양소 섭취 상태와 식사의 질 평가)

  • Kim Mi-Hyun;Bae Yun-Jung;Lee Da-Hong;Cho Hye-Kyung;Choi Sun-Hae;Sung Chung-Ja
    • Korean Journal of Community Nutrition
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    • v.10 no.6
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    • pp.869-879
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    • 2005
  • The purpose of this study was to evaluate on nutrient intake status and diet quality of middle and old aged vegetarian women in Korea, The research group was composed of vegetarian women (n=91), all of them were Seven Day Adventists, who had been on vegetarian diets, over 20 yrs. Their anthropometric measurements, dietary intakes, and diet quality indices were compared to omnivores (n=122). The average age of vegetarians and omnivores were 61.8 yrs and 60.3 yrs, respectively. The mean daily energy intakes of vegetarians and omnivores were 1428.8 kcal and 1424.5 kcal, respectively and there was no significant difference. The vegetarians consumed significantly lower intakes of protein (p < 0.05), zinc (p < 0.001) and vitamin $B_{2}$ (p < 0.05) compared to omnivores. In the diet quality, zinc and vitamin $B_{2}$ nutrient adequacy ratio (NAR) of vegetarians were significantly lower than those of omnivores. Mean adequacy ratio (MAR) of vegetarians and omnivores were 0.80 and 0.77, respectively and there was no significant difference. The KDDS (Korean's Dietary Diversity Score) of vegetarians and omnivores were 3.7 and 4.0, respectively, and there was significant difference (p < 0.01). The KDQI (Korean's Diet Quality Index) of vegetarians (1.5) was significantly lower than that (2.1) of omnivores (p < 0.001). In conclusion, vegetarian women have low intake status of protein, zinc and vitamin $B_{2}$, and partly low diet quality. Therefore it was needed that well planed diets to replace the nutrients supplied from excluded food groups in middle and old aged vegetarian women. (Korean J Community Nutrition 10(6) : $869\∼879$, 2005)

A Nobel Video Quality Degradation Monitoring Schemes Over an IPTV Service with Packet Loss (IPTV 서비스에서 패킷손실에 의한 비디오품질 열화 모니터링 방법)

  • Kwon, Jae-Cheol;Oh, Seoung-Jun;Suh, Chang-Ryul;Chin, Young-Min
    • Journal of Broadcast Engineering
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    • v.14 no.5
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    • pp.573-588
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    • 2009
  • In this paper, we propose a novel video quality degradation monitoring scheme titled VR-VQMS(Visual Rhythm based Video Quality Monitoring Scheme) over an IPTV service prone to packet losses during network transmission. Proposed scheme quantifies the amount of quality degradation due to packet losses, and can be classified into a RR(reduced-reference) based quality measurement scheme exploiting visual rhythm data of H.264-encoded video frames at a media server and reconstructed ones at an Set-top Box as feature information. Two scenarios, On-line and Off-line VR-VQMS, are proposed as the practical solutions. We define the NPSNR(Networked Peak-to-peak Signal-to-Noise Ratio) modified by the well-known PSNR as a new objective quality metric, and several additional objective and subjective metrics based on it to obtain the statistics on timing, duration, occurrence, and amount of quality degradation. Simulation results show that the proposed method closely approximates the results from 2D video frames and gives good estimation of subjective quality(i.e.,MOS(mean opinion score)) performed by 10 test observers. We expect that the proposed scheme can play a role as a practical solution to monitor the video quality experienced by individual customers in a commercial IPTV service, and be implemented as a small and light agent program running on a resource-limited set-top box.

Therapeutic Effect of Injection-Acupuncture with Bee-Venom (Apitoxin) in Cases of Canine Otitis Externa

  • Kim, Sang-Hun;Jun, Hyung-Kyou;Kim, Suk;You, Myung-Jo;Jun, Moo-Hyung;Kim, Duck-Hwan
    • Journal of Veterinary Clinics
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    • v.25 no.3
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    • pp.159-164
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    • 2008
  • This study was carried out to determine the therapeutic effect of injection-acupuncture (AP) with bee-venom (apitoxin) in cases of canine otitis externa (COE). Fifteen dogs with naturally-acquired otitis externa were used in this study. The dogs were divided into the following antibiotics group (control group), apitoxin group (experimental group A) and apitoxin combined with antibiotics group (experimental group B). All groups were treated by ear cleaning with normal saline once on day 1. The control group was treated with susceptible antibiotics, and experimental group A was given injection-AP with apitoxin $(100{\mu}g/head)$ at TH17 (Yi Feng), SI19 (Ting Gong), GB03 (Shang Guan) and TH03 (Zhong Zhu) bilaterally. Experimental group B was treated with susceptible antibiotics and injection-AP with apitoxin at the same acupoints as experimental group A. All the groups were treated 3 times/week for 2 weeks. The identity of the causative agents, the changes in the clinical signs, otoscopic findings, bacterial count in the auricular discharges, and total WBC counts and neutrophil/lymphocyte (N/L) ratio in the peripheral blood were investigated in all groups. In bacterial isolation, Staphylococcus spp. combined with Streptococcus spp. was detected higher than other agents. The bacterial cell count in experimental group A was significantly decreased at 2 weeks (p<0.01), and those in experimental group B was significantly decreased at 1 week (p<0.01) and 2 weeks (p<0.01) compared by those of control group, respectively. The changes of clinical score in experimental group B were significantly decreased at 2 weeks (p<0.01) compared by those of control group, but, those of experimental group A was similar to those of control group. The changes of total WBC counts and neutrophil/lymphocyte (N/L) ratio were no significant difference found. In conclusion, injection-AP with apitoxin is an effective treatment for COE and might be an alternative method for treating COE.

The Dietary Status of Kindergarten Child from a High Socioeconomic Apartment Compound in Seoul (일부(一部) 고소득(高所得) 아파트 단지내(團地內) 유치원(幼稚園) 어린이의 성장발육(成長發育) 및 영양(營養)에 관(關)한 연구(硏究))

  • Hyun,, Wha-Jin;Mo, Su-Mi
    • Journal of Nutrition and Health
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    • v.13 no.1
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    • pp.27-36
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    • 1980
  • A dietary survey of 100 children, aged 5 to 6, of Y.C. Kindergarten located in Yowido apartment compound of Seoul, was conducted July 18 to August 21, 1979. The results are summarized as follows: General family environment: Ninety four percent of the subjects were from families having two to three children, without grandparents in the home. One hundred percent of fathers were educated at or above the college level, while 83.9% of mothers had completed college. Mothers' ages ranged from 30 to 40 years, with 58% in the $31{\sim}35$ age bracket. Nearly 60% of the fathers were office and government employees, 10% were professors and teachers, 30% were engaged in businesses and other work, while 91.3% of mothers were unemployed. Anthropometric measurements: Mean values of standing height, body weight, girth of head and girth of chest, ranged from the 75th to the 90th percentile of Korean standards. None of the subjects had anthropometric indicators below the 5th percentile, representing the malnutrition borderline, of the Korean standards; while 12%, 24%, 17% and 14% of subjects supposed the 97th percentile in standing height, body weight, girth of head, and girth of chest, respectively. Furthermore, 58% of subjects exceeded Jelliffe's standard in arm circumferences; 60%, in triceps skinfold thickness; and 14.5% of subjects exceeded 120% of the latter standard. From this data, a certain number of these children were judged to he rather overweight. Dietary intake: Mean intake per day of energy and of nutrients, excluding iron, reached or exceeded Korean recommended dietary allowances. The energy input ratio of carbohydrate: protein: fat was 61.4 : 13.2 : 25.4, showing lower dependance on carbohydrates and higher dependance on fat for energy needs, compared with the average for Korean children of the same age group. Mean FAO-protein score of each subject's diet was shown to be as high as 90.6. Energy intake was divided among breakfast, lunch, and evening meal, on the average, in a ratio of 19.6 : 24.1 : 23.0. Approximately 33.3% of the daily caloric intake came from the snacks. Nutrition Knowledge of mothers: Nutrition knowledge of mothers was highly scored. Mothers' self-evaluations of their knowledge of nutrition was highly correlated with attitudes toward nutrition behavior.

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