• YAKHAK HOEJI
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• v.43 no.6
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• pp.729-735
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• 1999

Scopolia rhizome is mistaken as an atractylodes rhizome because of their similarities in shape. That is why atractylodes rhizome imported from China sometimes contain scopolia rhizome, which is very toxic. 8 persons were intoxicated atractylodes after taking imported atractylodes rhizome which is tainted. In kampo medicine prepared with such imported atractylodes rhizome, the level of tropane alkaloids ranged from 1.12∼4.34 mg/dose. In this study, we tried to investigate the tissue distribution of scopolia rhizome in rats. The extracts of scopolia was administered orally to rats (a single dose of 10mg/kg, 20mg/kg and 7 days repeated dose of 10mg/kg). Their blood was collected at 0.5, 1, 2, 4, 6 hrs, and liver, kidney, lung and spleen were collected after 6 hrs. The tissue homogenate was applied to solid phase extraction column for the determination of tropane alkaloids. After the oral administration of 20mg/kg scopolia extracts, l-hyoscyamine was detected in rat blood to 2 hrs after dosing. The concentration of tropane alkaloids was the highest in liver followed by lung, kidney and spleen. However, lung, kidney and spleen were similar in amount.

• Korean Journal of Plant Taxonomy
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• v.31 no.3
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• pp.267-282
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• 2001

To examine the leaf epidermal microstructure of three genera (Scopolia s.s., Anisodus, AtroPanthe, including Przewalskia as an outgroup) in the genera Scopolia Jacq. s.l., leaves of 10 species (37 specimens) were investigated by the light microscopy (LM) and scanning electron microscopy (SEM). The stomata of studied taxa were 'amphistomatic type' and the size (guard cell) range was $18-64{\times}11-48{\mu}m$. The size of stomata is slightly differed from between the taxa; the smallest size of stomata were found in the monotypic genus, Przewalskia ($24-27{\times}16-17{\mu}m$), on the other hand the largest one was found in Anisodus carniolicoides ($62-64{\times}43-48{\mu}m$). The stomatal complex was mostly anomocytic (in Scopolia s.s., Anisodus taxa : A. luridus, A. carniolicoides, A. acutangulus) and sometimes anisocytic (in Anisodus tanguticus, Przewalskia, Atropanthe). The stomata is mostly crescent in shape, but rarely circular, especially in Przewalskia tangutica. The shapes of epidermal cells are similar in both adaxial and abaxial sides, and mostly undulate/sinuate polygonal anticlinal wall, but rarely arched in Przewalskia tangutica. The epicuticular wax was not well developed in most studied taxa, except Anisodus tanguticus which is well developed cuticular striae around the stomatal complex. The elongate-headed glandular trichomes were found in Scopolia s.s. and Przewalskia. While the taxa of Anisodus and Atropanthe have not any trichomes (i. e., glabrous), except Anisodus luridus, which has simple or sometimes branched (dendritic- type) non-glandular trichome. Finally, the systematic and ecological significance of the leaf micromorphological features (stomata complex, trichome, etc.) in identification and elucidation of Scopolia s.l. including Przewalskia, especially between or within the genera including among the species is also discussed.

• Korean Journal of Plant Taxonomy
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• v.41 no.3
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• pp.271-279
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• 2011

The morphological characteristics of the Korean Scopolia japonica Maxim., commonly known as 'Mi-chi-gwang-i-pul' in Korean, were re-evaluated in comparison with results from previous research, while the distribution map was formulated according to existing flora and voucher specimens. The result of the re-evaluation has shown that there are some distinctive discrepancies compared to the previous reports of some characteristics such as the color of the basal stem, numbers of lateral veins, apex shape of calyx and corolla including the color of corolla. The Korean Scopolia japonica Maxim is found throughout Korea except Jeju-do province. The intra-specific species such as S. parviflora (Dunn.) Nakai, S. lutescens Y.N. Lee and S. kwangdokensis Y.N. Lee were treated as synonyms of S. japonica Maxim. in this research.

• Korean Journal of Plant Taxonomy
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• v.46 no.1
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• pp.60-64
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• 2016

Scopolia parviflora of the family Solanaceae is an endemic species of Korea and a traditional Korean medicinal plant. The plastid genome was sequenced by next-generation sequencing (NGS) method. The characterized cp genome is 156,193 bp in size; the large single-copy (LSC) region is 86,364 bp, the inverted repeat (IR) is 25,905 bp, and the small single copy (SSC) region is 18,019 bp. The overall GC content of the plastid genome amounts to 37.61%. The cp genome contains 113 genes and 21 introns, including 80 proteincoding genes, four RNA genes, 30 tRNA genes, 20 group II introns, and one group I intron. A phylogenetic analysis showed that Scopolia parviflora was closely related to Hyoscyamus niger.

• Journal of The Korean Society of Clinical Toxicology
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• v.2 no.1
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• pp.37-40
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• 2004

In emergency department, the clinicians occasionally meet some patients with toxicity caused by ingestion of unknown wild edible greens or herbal agents. Due to there was few quantitative or qualitative studies about the mechanism or clinical features of these materials, it' s hard to approach to the patients and start the initial management in emergency department. Authors experienced the case of poisoning by Scopolia Japonica ingestion. We report this case with review of Scopolia Japonica.

• YAKHAK HOEJI
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• v.1 no.1
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• pp.6-9
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• 1948

국산 Scopolia parviflora Nakai '미치광이'를 생약적으로 연구하여 우리 약국방제정에 있어 '스코폴리아' Rhizoma Scopliae의 원식물오 규정할수 있는가 없는가를 천명함에 있다.

• The Journal of the Society of Stroke on Korean Medicine
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• v.15 no.1
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• pp.50-56
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• 2014

The prevalence rate of plant poisoning is growing up gradually. However, it could be difficult to get proper treatment without detail medical history taking due to the diversity of poisonous plants and their various symptoms. Scopolia Japonica, which grows naturally in Korea, is reported to cause severe toxicity which could be lethal. A few therapeutic measures have been reported in Traditional Korean Medicine (TKM), but it has not been reported yet that those measures have been successfully proven in the clinical practice in modern times. This is a case report of toxicity caused by scopolia japonica ingestion. A 65years-old male patient with cognitive impairment, behavioral disorder, gait difficulty, dysarthria, dysphagia and abnormal pupil reflex recovered from those symptoms during taking Gamigamdu-tang and getting acupuncture. This study shows the clinical aspects of Scopolia Japonica toxicity and the possibility of TKM treatments as one of plant poisoning treatments.

• Journal of Plant Biology
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• v.36 no.3
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• pp.225-231
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• 1993

Transformed hairy roots of Scopolia parviflora, producing tropane alkaloids and native to Korea, were obtained following infection of rhizome segments with Agrobacterium rhizogenes A4. Each root tip induced from inoculum sites excised and cultured in MS agar or liquid medium. About seventy of hairy root clones were established. Among them, several fast growing hairy root clones were examined for alkaliod content. Two dimensional TLC analysis showed that the tropane alkaloid pattern of hairy root was more complicated than that in the rhizome of mother plant. On the other hand, some hairy root clones did not produce scopolamine and hyoscyamine. In HPLC analysis, some hairy root clones yield higher levels of scopolamine and hyoscyamine than those of mother plant rhizome which used for infection. Scopolamine and hyoscyamine were identified by comparison of their retention times and of their spectra data with those of authentic compounds.

• Korean Journal of Plant Taxonomy
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• v.33 no.4
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• pp.373-386
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• 2003

Internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA were determined for 14 individuals representing eight taxa from Scopolia s. str. and related genera, Anisodus and Atropanthe. We found that the ITS sequences of Korean endemic species, S. parviflora, are significantly different from its allied species, S. japonica. This is contradictory to traditional taxonomic treatments in which those species are regarded as conspecific. S. parviflora exhibited closer relationship to S. carniolica, which is disjunctly distributed in Europe. In spite of substantially high sequence divergence between S. japonica and S. parvlflora/S. carniolica clade, morphological resemblance is evident among the species. Morphological stasis concept (retardation of morphological differentiation or evolution of similar characters among the disjuncts in a similar ecological habitat) was referred to understand this rather unusual evolutionary feature. S. lutescens, another Korean endemic species, shared almost identical ITS sequences with S. parviflora. Lack of diagnostic character distinguishing the taxa suggests that they are conspecific. Anisodus carniolicoides, which was originally described in Scopolia, was grouped with A. luridus and A. tanguticus. The monophyletic Anisodus formed a sister group relationship with a monotypic genus Atropanthe.

• Korean Journal of Medicinal Crop Science
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• v.12 no.2
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• pp.135-140
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• 2004

Total RNAs were isolated from cultured roots of Scopolia parviflora, $poly(A)^+$ RNA was obtained through the mRNA purification, cDNA library of Hnh6h was constructed. Recombinant baculoviruses in Spodoptera frugiperda (Sf) cells were constructed by use of the transfer vector pBacPAK, which has the AcNPV sequence under the polyhedrin promoter. The expression vector carrying Hnh6h gene was transferred to S. parviflora and obtained transgenic hairy root lines. Our results confirmed the over expression of the H6H protein was used by anti-pBacPAK about cDNAs of S. parviflora. This study will served for production of tropane alkaloids by metabolic engineering.