• 생약학회지
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• 제52권4호
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• pp.208-211
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• 2021

Four compounds were isolated from 90% ethanol extract of the stem of Clematis heracleifolia. On the basis of spectral data, the structure of isolated compounds were identified as sitosterone (1), β-sitosterol (2), scoparone (3) and octadecanoyl caffeate (4), respectively. Sitosterone (1) and octadecanoyl caffeate (4) are isolated from this plant for the first time.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.168-168
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• 1996

가는잎쇄기풀(Urtica angustifolia Fisch.)은 쇄기풀과(Urticaceae)에 속하는 다년생 초본으로 중약 또는 민간에서 동속 근연식물과 함께 전초를 담마라하여 류마치스성 동통, 산후의 산풍, 소아의 추풍, 경풍,담마진의 치료에 사용되고 있다. Rat에서 실험적 항당뇨 효과를 검토해본 결과 혈당강하 작용이 있는 본 식물로부터 그 혈당강하 성분의 분리에 앞서 식물화학 성분을 규명하고자 본 실험에 착수하였다. 가는잎쇄기풀 전초의 MeOH ex.를 CH$_2$Cl$_2$, EtOAc, n-BuOT 및 $H_2O$로 분획하고 각종 column chromatography를 통하여 다수의 화합물을 분리하였다. 각 화합물은 이화학적 성상 및 spectral data로부터 scopoletin, esculetin dimethyl ether(scoparone), sterol mixture, $\beta$-sitosteryl-3-o-glucoside, kaempferol-3-o-glucoside, quercetin-3-o-glucoside, kaempferol-3-o-rutinoside로 확인하였으며 그 외 다수의 화합물은 그 구조를 규명중이다.

• 생약학회지
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• 제46권1호
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• pp.6-11
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• 2015

To clarify chemical constituents of Clematis heracleifolia, isolation and structure elucidation of the underground parts of C. heracleifolia were performed. Five compounds were isolated from $CHCl_3$ and n-BuOH soluble fraction of this plant. On the basis of spectral and physico-chemical data, the structure of isolated compounds were identified as coniferyl alcohol (1), scoparone (2), (+)-lariciresinol (3), phytosterols (4), and daucosterol (5), respectively. All compounds are isolated from this plant for the first time. To evaluate anti-acetylcholinesterase activity of the isolated compounds, compounds 1, 2, 3, and 5 were tested inhibition activity against acetylcholinesterase. Among tested compounds, daucosterol (5) showed acetylcholinesterase inhibitory activity with $IC_{50}$ value of $6.1{\mu}M$.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.335-338
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• 2020

Artemisia iwayomogi (Compositae), a perennial mugwort, is native to Korea and widely distributed in Japan, Russia, and China. A. iwayomogi and A. capillaris are similar in morphology and pharmacological activity and have been used for the same medicinal purposes in Korea. While various ingredients such as coumarins and flavonoids and their activity studies have been reported for A. capillaris, few studies have been conducted on the pharmacologically active components of A. iwayomogi. In Korea, A. capillaris is not economical because only young leaves are used as a medicinal material. Because of this, A. iwayomogi is frequently used in Korea, indicating the need to study its pharmacologically active components. Therefore, a phytochemical study was initiated to isolate active compounds from the aerial parts of A. iwayomogi. Finally, four coumarins, umbelliferone (1), esculetin (2), grevillone (3), and scoparone (4) were isolated for the first time from the aerial parts of A. iwayomogi in this study.

• 대한한방부인과학회지
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• 제33권2호
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• pp.1-12
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• 2020

Objectives: This study was conducted to find out the optimal extraction time for Artemisia argyi. Methods: The compositional pattern was compared with HPLC (High Performance Liquid Chromatography) and GC (Gas-Chromatography) by decocting Artemisia argyi 10, 60, 120 minutes respectively. Results: With longer extraction time, the contents of reference compounds were extracted 1.1 times more when 3,4-dicaffeoylquinic acid was extracted for 60 minutes than when extracted for 10 minutes in HPLC test, but the contents were reduced when extracted for 120 minutes compared to 60 minutes extraction time. 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, jaceosidin, and eupatilin showed the largest yield rate when extracted for 10 minutes, and it decreased as time passed. The contents of chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, jaceosidin, scoparone, and eupatilin were detected only in 10 minutes extraction but not in 60 or 120 minutes extraction according to GC test. Conclusions: The results show that extraction time could affect the physicochemical characteristic or composition of Artemisia argy extracted. Thus, short extraction time could be useful for decoction of Artemisia argyi.

• 동의생리병리학회지
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• 제20권2호
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• pp.455-459
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• 2006

The inhibitors of prostaglandin $E_2\;(PGE_2)$ production and cyclooxygenase-2 (COX-2) activity have been considered as potential anti-inflammatory agents. In this study, we evaluated 9 compounds isolated from 5 Korean phytomedicinal plants (Spirea prunifolia, Paeonia suffruticosa, Salvia miltiorrhiza, Scutellaria baicalensis, and Artemisia capillaris) for the inhibition of $PGE_2$production and COX-2 expession in lipopolysaccharide (LPS)-stimulated human macrophages U937 cells. As a result, several compound such as prunioside A, penta-O-galloyl-beta-D-glucose, tanshinone IIA, baicalin, baicalein, wogonin, scopolatin, scoparone and decursinol showed potent inhibition of $PGE_2$production (50-70% inhibition at the test concentration of $10\;{\mu}M$). In addition, these compounds were also considered as potential inhibitors of COX-2 activity (45-73% inhibition at the test concentration of $10\;{\mu}M$). These active compound mediating COX-2 inhibitory activities are warranted for further elucidation of active principles for development of anti-inflammatory agents and these properties may contribute to the anti-atopic dermatitis activity.