• Journal of Plant Biology
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• v.36 no.3
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• pp.281-284
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• 1993

A karyotype of cytotype BB plant in Scilla scilloides Complex was established and the frequency of B-chromosomes were investigated. Chromosome complements of BB genome were composed of five pairs of subtelocentric and four pairs of metacentric chromosomes. Chromosome 1 has satellite with nucleolar organizer. Polymorphism was found in chromosome 2. The karyotype of cytotype BB will be available for analysis of genome composition in various cytotypes of S. scilloides Complex. The frequency of B-chromosome was 78.6%. Numbers of B-chromosome ranged from 1 to 4 and plants with 2B-chromosomes were predominant (57.2%). Two type of B-chromosomes, F and F', were found; F is a large iso-chromosome and F' a small one.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.235-240
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• 1999

The usefulness of randomly amplified polymorphic DNA (RAPD) was evaluated to access the genetic variation in somaclones derived from different cytotype plants of Scilla scilloides Complex, AA (2n=16), BB (2n=18) and AABB (2n=34). Three arbitrary decamer primers were successfully used to amplify genomic DNA from the somaclones. DNA polymorphism was observed between cytotypes. The total number of bands in AA, BB and AABB somaclones were 110, 116 and 103, and marker bands examined were 15, 19 and 26, respectively. The diversity of types using PCR in AA, AABB and BB somaclones were 39.2%, 72.3% and 45.7%, respectively. RAPD band patterns suggest that type AA is more stable than type BB and AABB. The frequencies of specific band in AA, BB or AABB somaclones were 0.9%, 4.3% and 4.9%, respectively. The applicability and reliability of RAPD markers for evaluating the somaclones are discussed.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.59-63
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• 1994

Chromosome variation in callus cells initiated from different cytogenetic type plane of Scilla scilloides Complex was analysed Considerable variation in both chromosome number and struchure was found in type AA, while no autosomal variation was detected in type BB callus cells. In allotetraploid AABB, two hypoploid cells were fount while a hypoploid cell and three hyperploid cells were found in eutetraploid cells of BBBB. Autosomes in callus cells derived from the plant with B-chromosomes were more stable than those from the plant without B-chromosome. We doubt that B-chromosomes have a selective function for the autosomes in culture of S. scilloides Complex.

• Journal of Plant Biology
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• v.33 no.4
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• pp.237-242
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• 1990

Geographical distribution of diploid plant with AA genome (2n=16) and allotetraploid with AABB genome (2n=34) of Scilla scilloides Complex from Korea has been studied. The composition and frequencies of B chromosomes ere also investigated. Plants with AABB genome were predominant over AA genome plants. A mixed population of AA and AABB genome plants was found for the first time. Aneuploid plants have not been found. Chromosomes of AA genome were composed of three pairs of metacentric, two pairs of submetacentric, two pairs of subtlocentric and one pair of telocentric chromosomes, whereas BB genome was four pairs of metacentric and five pairs of subtelocentric chromosomes. B chromosomes were classified into two categories, isochromosome (F) and chromosome fragment (f). The frequencies of B chromosomes were 43% in AA genome plants and 44% in AABB genome plants. The number of B chromosome ranged from 1 to 3 and 1 to 7 in AA and AABB genome plants, respectively. B chromosome combinations were F and F+f in AA genome plants and F, F+f and f in AABB genome plants.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.242.1-242
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• 1994

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.170.1-170
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• 1994

• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.348.2-348
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• 1995

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.257.2-257
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• 1996

• Journal of Plant Biology
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• v.34 no.2
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• pp.145-150
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• 1991

제주도에서 서식하고 있는 무릇의 게놈 분포와 B염색체의 출현 빈도를 조사하였다. 게놈의 유형은 BB, ABB, ABBB, AABB, AABBB 및 AAABBB의 6가지로 나타났으며, 그 중 BB, ABB 및 AAABBB게놈 식물은 제주도 집단에서 처음으로 발견하여 보고되는 것이다. 게놈의 분포는 AABB가 66.2%로 가장 높게 나타났으며, ABBB게놈이 20.4%로 그 다음이었다. ABB와 AAABB게놈 식물은 한 개체씩만 발견되었다. 게놈의 조성은 조사된 13개 집단 중 세가지 유형이 혼생하는 집단이 2개, 두 가지 유형이 나타나는 집단이 BB, AABB 또는 AABBB 중 한 가지 유형의 게놈 식물이 분포하고 있었다. 게놈의 조성으로 보아 제주도의 무릇 집단은 한반도 내륙보다는 일본 집단과의 유연 관계가 더 가까운 것으로 생각된다. B염색체의 수는 1-4개로 나타났으며, 모두 등완염색체(F)였다. B염색체의 출현 빈도는 BB와 AABBB게놈 식물에서 각각 75%로 가장 높게 나타났으며, AABB게놈 식물에서는 48%로 나타나 한반도 내륙(44%)과 비슷한 양상을 보였다. A게놈에 비해 B게놈의 수를 많이 지닌 식물에서 B염색체의 빈도가 높게 나타나는 것이 특징으로 나타났다.