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Antioxidant Activity of Allium hookeri Root Extract and Its Effect on Lipid Stability of Sulfur-fed Pork Patties

  • Cho, Han-Seul;Park, Woojoon;Hong, Go-Eun;Kim, Ji-Han;Ju, Min-Gu;Lee, Chi-Ho
    • Food Science of Animal Resources
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    • v.35 no.1
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    • pp.41-49
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    • 2015
  • This study was performed to assess the antioxidant activity of Allium hookeri root extract (AHE) on lipid oxidation of raw sulfur-fed pork patties for 14 d of refrigerated storage. Different concentration of ethanol (0-100%) and time (1-12 h) were applied to determine the extraction condition. Water (0% ethanol) extraction for 1 h was selected as an optimal extraction condition of AHE for the following study showing the highest total phenolic content and total flavonoid content, as well as the strongest antioxidant activity. The 1% AHE (SP1), 3% AHE (SP2), and 0.05% ascorbic acid (SP3) were added into sulfur-fed pork patties against controls; SP0 (sulfur-fed pork patties with no AHE) and P0 (normal pork patties with no AHE). The pH values of P0 and SP0 significantly increased (p<0.05) than others on 14 d and redness of P0 showed the largest decrement during storage. P0 and SP0 showed higher production of conjugated dienes on d 7 than others (p<0.05). Thiobarbituric acid reactive substances (TBARS) values were decreased in proportion to the increased level of AHE on 14 d (p<0.05) resulting in higher TBARS values on P0 and SP0 (p<0.05) and the negative correlation between AHE level and TBARS were also demonstrated (r=-0.910, p=0.001). Therefore, the results suggest that AHE effectively retarded the lipid oxidation rate of sulfur-fed pork patties indicating the potential usage of AHE as a natural preservative.

The Horse Manure Fertilizers and Pollutants Characteristics Analysis Research (말 분뇨의 비료성분 및 오염물질 특성 조사 연구)

  • Kwag, J.H.;Jeong, M.W.;Choi, D.Y.;Kim, J.H.;Kim, Y.H.;Park, J.C.;Kim, I.C.;Yang, C.B.;La, C.C.
    • Journal of Animal Environmental Science
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    • v.18 no.3
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    • pp.201-206
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    • 2012
  • Horse manure general composition and contaminants, heavy metals content analysis was investigated as follows. The moisture content of horse feces was 76.1% and 96% for horse urine. The average T-N, T-P, T-K concentrations of the racing horse were 0.18%, 0.10%, 0.22% respectively and 2.88%, 0.0015%, 0.84% for urine. And the average BODs, COD, SS concentrations were 26,906 $mg/{\ell}$, 36,642 $mg/{\ell}$, 89,375 $mg/{\ell}$ respectively and 14,298 $mg/{\ell}$, 7,484 $mg/{\ell}$, 6,987 $mg/{\ell}$ for urine. In addition, the Cr, Cu, Ni, Cd, Pb, Zn concentrations of feces were 2.65 mg/kg, 7.05 mg/kg, 1.24 mg/kg, 0.07 mg/kg, 0.01 mg/kg, 45.11 mg/kg respectively and 1.52 mg/kg, 1.62 mg/kg, 0.00 mg/kg, 0.03 mg/kg, 0.03 mg/kg, 4.49 mg/kg for urine on the fresh matter basis. The end of the survey was that the quality of the compost can be used as a raw material, and manure is necessary to constantly driven forward horse manure composting research.

Organic Acids and Volatile Compounds Isolated from Prunus mume Extract (매실추출물로부터 분리된 유기산과 휘발성분)

  • Ha Myung-Hee;Park Woo-Po;Lee Seung-Cheol;Cho Sung-Hwan
    • Food Science and Preservation
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    • v.12 no.3
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    • pp.195-198
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    • 2005
  • Among organic acids, Prunus mume extract(PME) was mostly consisted of $0.47\;mg\%$ citric, $0.43\;mg\%$ malic and $0.25\;mg\%$ oxalic acid Volatile compounds in PME were identified by GC/MSD, showing that acetic acid($8.3\%$) and p-coumaric acid($13.1\%$) as well as 5-hydroxymethyl furfural($32.3\%$), furfural($8.3\%$), and 3-methyl-2,3-furandione($2.3\%$) were major compounds which are known as antimicrobial substances.

Protein Quality Evaluation of Cooked Hagfish (Eptatretus burgeri) Meats

  • Hwang, Eun-Young;Lee, Jin-Hwa;Ryu, Hong-Soo;Park, Nam-Gyu;Chun, Soon-Sil
    • Preventive Nutrition and Food Science
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    • v.7 no.3
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    • pp.287-292
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    • 2002
  • The effect of cooking methods on in vivo and in vivo indices of the protein quality of hagfish meat were investigated. In vivo protein digestibilities of cooked meats (81.3~83.5 %) were not significant different (p<0.05) from those of van meat (82.9%), with the exception of steamed (11$0^{\circ}C$, 15 min) meat (86.3 %). Convection oven cooking (22$0^{\circ}C$, IS min) resulted in a higher trypsin indigestible substrate (TIS, 49.2 mg/g solid) compared with that of raw meat (38.9 mg/g solid). free amino acid content of raw meat was decreased after boiling (10$0^{\circ}C$, 10min). Both convection oven and microwave cooking (2,450 MHz, 3 min) decreased available lysine from 4.9g/16g N to 3.8~4.1g/16g N. In vivo apparent protein digestibilites (AD) of hagfish meat were similar fur raw (92.4%) and cooked meats, but were somewhat lower than ANRC (Animal Nutrition Research Council) casein (945%). The PERs (3.7~4.1) and NPRs (3.7~4.9) of cooked meats were significantly higher (p<0.05) than those of raw meat (PER 3.3, NPR 3.6 and ANRC casein (PER 2.5, NPR 2.6), despite their lower in vivo protein digestibilities. These results demonstrate that cooking at optimal conditions resulted in remarkably positive effects on in vivo and in vivo protein qualities of hagfish meats. Therefore, steamed hagfish meat is an excellent source of high quality protein from seafood products.

Wear Behaviors of Gas Atomized and Extruded Hypereutectic Al-Si Alloys (가스분무 공정에 의한 과공정 Al-Si 합금 분말 압출재의 마모 거동)

  • Jin Hyeong-Ho;Nam Ki-Young;Kim Yong-Jin;Park Yong-Ho;Yoon Seog-Young
    • Journal of Powder Materials
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    • v.13 no.4 s.57
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    • pp.250-255
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    • 2006
  • Wear behaviors of gas atomized and extruded Al-Si alloys were investigated using the dry sliding wear apparatus. The wear tests were conducted on Al-Si alloy discs against cast iron pins and vice versa at constant load of 10N with different sliding speed of 0.1, 0.3, 0.5m/s. In the case of Al-Si alloy discs slid against the cast iron pins, the wear rate slightly increased with increasing the sliding speed due to the abrasive wear occurred between Al-Si alloy discs and cast iron pins. Conversely, in the case of cast iron discs against Al-Si alloy pins, the wear rate decreased with increasing the sliding speed up to 0.3m/s. However, the wear rate increased with increasing the sliding speed from 0.3m/s to 0.5m/s. It could be due to adhesive wear behavior and abrasive wear behavior_between cast iron discs and Al-Si alloy pins.

An Empirical Study of the Analytical Measurement Range in Clinical Chemistry (분석측정범위의 실증적 평가)

  • Chang, Sang-Wu;Lee, Sang-Gon;Kim, Young-Hwan;Song, Eun-Young;Park, Yong-Won;Park, Byong-Ok;Lyu, Jae-Gi
    • Korean Journal of Clinical Laboratory Science
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    • v.38 no.2
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    • pp.117-124
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    • 2006
  • The analytical measurement range (AMR) is the range of analyte values that a method can directly measure on a specimen without any dilution, concentration, or other pretreatment not part of the usual assay process. The linearity of the AMR is its ability to obtain test results which are directly proportional to the concentration of analyte in the sample from the upper and lower limit of the AMR. The AMR validation is the process of confirming that the assay system will correctly recover the concentration or activity of the analyte over the AMR. The test specimen must have analyte values which, at a minimum, are near the low, midpoint, and high values of the AMR. The AMR must be revalidated at least every six months, at changes in major system components, and when a complete change in reagents for a procesure is introduced; unless the laboratory can demonstrate that changing the reagent lot number does not affect the range used to report patient test results. The AMR linearity was total protein (0-16.6), albumin (0-8.1), total bilirubin (0-18.1), alkaline phosphatase (0-1244.3), aspartate aminotransferase (0-1527.9), alanine aminotransferase (0-1107.9), gamma glutamyl transpeptidase (0-1527.7), creatine kinase (0-1666.6), lactate dehydrogenase (0-1342), high density lipoprotein cholesterol (0.3-154.3), sodium (35.4-309), creatinine (0-19.2), blood urea nitrogen (0.5-206.2), uric acid (0-23.9), total cholesterol (-0.3-510), triglycerides (0.7-539.6), glucose (0-672.7), amylase (0-1595.3), calcium (0-23.9), inorganic phosphorus (0.03-17.0), potassium (0.1-116.5), chloride (3.3-278.7). We are sure that materials for the AMR affect the evaluation of the upper limit of the AMR in the process system.

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Microstructure Observations in $(1-x)NdAlO_3-xCaTiO_3$ System ($(1-x)NdAlO_3-xCaTiO_3$ 시스템의 미세구조 관찰)

  • Lee, Hwack-Joo;Ryu, Hyun;Park, Hyun-Min;Cho, Yang-Koo;Kim, Jae-Chun;Nahm, Sahn
    • Applied Microscopy
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    • v.32 no.1
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    • pp.9-15
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    • 2002
  • Microstructural investigations of $(1-x)NdAlO_3-xCaTiO_3$ (NACT) complex perovskite compounds were carried out using X-ray diffractometry, neutron diffraction and transmission electron microscopy. When $0.3{\leq}x{\leq}0.9$, NACT had not only the 1 : 1 chemical ordering of cations but also the antiphase and inphase tilting of oxygen octahedron and the antiparallel shift of cations. Both the antiphase boundaries and the ferroelastic domains were present in the microstructure. The long and straight ferroelastic domains became degenerate as x decreases. When x was smaller than 0.3, the chemical ordering was absent and the antiphase tilting of oxygen octahedron was observed. The defects like tangled dislocations and the second phase were also found in the microstructure.

Quantitative Analysis of Residual 24 Organochlorine POPs in Sundried Salts (천일염 중 유기염소계 잔류성 유기오염물질(POPs) 잔류분석)

  • Choi, Geun-Hyoung;Park, Mi-Ran;Park, Jong-Min;Hong, Su-Myeong;Kwon, Oh-Kyoung;Park, Yun-Ki;Kim, Jin-Hyo
    • The Korean Journal of Pesticide Science
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    • v.15 no.4
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    • pp.502-506
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    • 2011
  • Most countries have the legislation and regulation for POPs control in food. In here, we studied the quantitative analysis of 24 organochlorine POPs (${\alpha}$-HCH 1, ${\beta}$-HCH 2, ${\gamma}$-HCH 3, ${\delta}$-HCH 4, trans-chlrodane 5, 2,4'-DDE 6, ${\alpha}$-endosulfan 7, cis-chlordane 8, 2,4'-DDD 9, endrin 10, ${\beta}$-endosulfan 11, 2,4'-DDT 12, endosulfan sulfate 13, HCB 14, aldrin 15, trans-nonachlor 16, 4,4'-DDE 17, dieldrin 18, 4,4'-DDD 19, cis-nonachlor 20, 4,4'-DDT 21, heptachlor 22, heptachlor epoxide 23 and mirex 24) with GC-ECD. The retention time of analytes were ranged between 19.18 min and 34.69 min, and their peak intervals were over 0.05 min at least. LOQs were ranged 0.003 ~ 0.033 ng/g, and their recovery rates were showed 60.9 ~ 120.8% on the 0.1 ng/g concentration of 24 organochlorine POPs. All tested 30 sundried salts were collected on Korean retailed market, and any analyte was not found in all the samples on LOQ levels.

Developmental Characteristics of Cloned Embryos Reconstructed with Induced Pluripotent Stem Cells in Pigs (돼지 유도만능줄기세포 유래 복제란의 특성 분석)

  • Kwon, Dae-Jin;Oh, Jae-Don;Park, Mi-Ryung;Hwang, In-Sul;Park, Eung Woo;Hwang, Seongsoo
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.3
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    • pp.232-239
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    • 2019
  • In general, cloned pigs have been produced using the somatic cell nuclear transfer (SCNT) technique with various types of somatic cells; however, the SCNT technique has disadvantages not only in its low efficiency but also in the development of abnormal clones. This study aimed to compare early embryonic development and quality of SCNT embryos with those of induced pluripotent stem cells (iPSCs) NT embryos (iPSC-NTs). Ear fibroblast cells were used as donor cells and iPSCs were generated from these cells by lentiviral transduction with human six factors (Oct4, Sox2, c-Myc, Nanog, Klf4 and Lin28). Blastocyst formation rate in iPSC-NT (23/258, 8.9%) was significantly lower than that in SCNT (46/175, 26.3%; p < 0.05). Total cell number in blastocysts was similar between two groups, but blastocysts in iPSC-NT had a lower number of apoptotic cells than in SCNT (2.0 ± 0.6 vs. 9.8 ± 2.9, p < 0.05). Quantitative PCR data showed that apoptosis-related genes (bax, caspase-3, and caspase-9) were highly expressed in SCNT than iPSC-NT (p < 0.05). Although an early development rate was low in iPSC-NT, the quality of cloned embryos from porcine iPSC was higher than that of embryos from somatic cells. Therefore, porcine iPSCs could be used as a preferable cell source to create a clone or transgenic animals by using the NT technique.

Characterization of Mushroom Tyrosinase Inhibitor in Sweet Potato (고구마에 존재하는 mushroom tyrosinase 저해제의 특성)

  • Lee Hyun-Ju;Lee Min-Kyung;Park In-Shik
    • Journal of Life Science
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    • v.16 no.3 s.76
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    • pp.396-399
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    • 2006
  • Crude extract prepared from sweet potato possessed inhibitory activity toward mushroom tyrosinase. The inhibitory activity was dependent upon the addition amount of sweet potato extract. After heating the sweet potato extract at $95^{\circ}C$ for 20 min, its inhibitory activity retained 37.3%. Its optimum activity was shown at pH ranges of 5.0-7.0. The tyrosinase inhibitory activity was disappeared by dialysis, which suggests the inhibitory activity of sweet potato extract is caused by some compounds of low molecular weight.