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The anti-obesity effect of Lethariella cladonioides in 3T3-L1 cells and obese mice

  • Sung, Ju-Hyun;Chon, Jeong-Woo;Lee, Mi-Ae;Park, Jin-Kyung;Woo, Jeong-Taek;Park, Yoo-Kyoung
    • Nutrition Research and Practice
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    • v.5 no.6
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    • pp.503-510
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    • 2011
  • The aim of this study was to investigate whether a water extract of L. cladonioides (LC) has an anti-obesity effect in 3T3-L1 cells and obese mice. Treatment of differentiated 3T3-L1 adipocytes with LC caused a significant increase in glycerol release and reduced the protein expression of the adipogenic transcription factors, $PPAR{\gamma}$ and C/$EBP{\alpha}$. In an animal model, obese mice were artificially induced by a high fat diet for 10 weeks. Experimental groups were treated with LC (100 mg/kg/day) by gavage for the next 10 weeks. At the end of experiment, the body weight of the LC group mice was reduced by 14.2% compared to the high fat diet (HFD) group. The treatment also decreased liver (31.0%), epididymal (18.0%) and retroperitoneal (19.3%) adipose tissue, and kidney (6.7%) weights, respectively, compared with those of the HFD group. LC prevented diet-induced increases in the serum level of TC (22.6%), TG (11.6%), and glucose (35.0%), respectively, compared with the HFD group. However, the HDL-C level was higher in the LC group (26.1%) than the HFD group. The results of this study thus suggest that LC suppressed lipid accumulation and expression of adipogenic transcription factors, and increased the amount of glycerol release. LC also indicated an anti-obese and anti-hyperlipidemic effect.

Electrochemical Reduction of 0,0-Dimethyl-0-(3-Methyl-4-Nitrophenyl)-phosphorothioate(Sumithion®) in Acetonitrile Solution (Acetonitrile 용액중(溶液中)에서 0,0-Dimethyl-0-(3-Methyl-4-Nitrophenyl)-phosphorothioate(Sumithion®)의 전기화학적(電氣化學的) 환원반응(還元反應))

  • Park, Seung Heui;Sung, Nack Do
    • Korean Journal of Agricultural Science
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    • v.11 no.2
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    • pp.315-321
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    • 1984
  • The electrochemical reduction of 0,0-dimethyl-0-(3-methyl-4 -nitrophenyl)-phosphorothioate ($Sumithion^{(R)}$) in acetonitrile solution has been studied by direct current (DC), differential pulse (DP) polarography and cyclic voltammetry methods. The irreversible electron-transfer chemical reaction (EC) mechanism of Sumithion proceeds by six electron-transfer to form radical and reduction of three-step which undergoes single bond of the phosphorus atom & phenoxy group by electron-transfer and protonation cleaved to give p-hydroxyamino-m-cresol and dimethylthiophosphonate as major product.

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Effect of Dietary Fermented Earthworm Cast Additives on Odor Reduction of Poultry House and Egg Production (지렁이 분변토 발효 사료 첨가제가 계란 생산 및 악취 저감에 미치는 영향)

  • Hwangbo, Jong;Park, Sang-O;Park, Byung-Sung
    • Korean Journal of Poultry Science
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    • v.41 no.1
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    • pp.1-5
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    • 2014
  • This study was carried out to investigate the effect of feeding fermented earthworm cast additives (FEC) on egg production, egg yolk fatty acids, blood lipid, cecal microorganism and fecal odor. The completely randomized experiment was applied, and the treatments were designed as 2 feeding regimens (control vs. FEC feeding), and FEC contained 3.5% top dressing to common diet. Egg production and egg weight of FEC group were significantly higher than that of control (p<0.05). Ratios of n-6/n-3 fatty acids in the egg yolk of FEC group were significantly higher than those in control group (p<0.05). Concentrations of HDL-C in FEC group was significantly higher than control group (p<0.05), but not triglyceride and total cholesterol. The caecal Lactobacillus of FEC group was significantly higher in FEC group than control group (p<0.05). $NH_3$ concentrations from poultry house were significantly lower in FEC group than control (p<0.05). In conclusion, these study results showed that the addition of FEC at 3.5% to the diet has reduced odor of poultry house and improved the n-6/n-3 fatty acid of egg yolk and egg production in laying hens.

Clinical performance of FractionLab in patient-specific quality assurance for intensity-modulated radiotherapy : a retrospective study

  • Oh, Se An;Kim, Sung Yeop;Park, Jaehyeon;Park, Jae Won;Yea, Ji Woon
    • Journal of Yeungnam Medical Science
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    • v.39 no.2
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    • pp.108-115
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    • 2022
  • Background: This study was aimed at comparing and analyzing the results of FractionLab (Varian/Mobius Medical System) with those of portal dosimetry that uses an electronic portal imaging device. Portal dosimetry is extensively used for patient-specific quality assurance (QA) in intensity-modulated radiotherapy (IMRT). Methods: The study includes 29 patients who underwent IMRT on a Novalis-Tx linear accelerator (Varian Medical System and Brain-LAB) between June 2019 and March 2021. We analyzed the multileaf collimator DynaLog files generated after portal dosimetry to evaluate the same condition using FractionLab. The results of the recently launched FractionLab at various gamma indices (0.1%/0.1 mm-1%/1 mm) are analyzed and compared with those of portal dosimetry (3%/3 mm). Results: The average gamma passing rates of portal dosimetry (3%/3 mm) and FractionLab are 98.1% (95.5%-100%) and 97.5% (92.3%-99.7%) at 0.6%/0.6 mm, respectively. The results of portal dosimetry (3%/3 mm) are statistically comparable with the QA results of FractionLab (0.6%/0.6 mm-0.9%/0.9 mm). Conclusion: This paper presents the clinical performance of FractionLab by the comparison of the QA results of FractionLab using portal dosimetry with various gamma indexes when performing patient-specific QA in IMRT treatment. Further, the appropriate gamma index when performing patient-specific QA with FractionLab is provided.

Characterization of Metal/Cobalt Ferrite Magnetic Thin Films

  • Park, C.H.;Na, J.G.;Heo, N.H.;Lee, S.R.;Kim, J.;Park, K.
    • Journal of Magnetics
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    • v.3 no.1
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    • pp.31-35
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    • 1998
  • Metal/cobalt ferrite composite thin films with the saturation magnetization (M_s$)of~580 emu/cm3 and the coercivity(Hc) of 1700 Oe were prepared by the reactive sputtering. With increasing substrate temperature, Ms of the thin films increased, while Hc of the thin films decrease. This sttributed to the precipitation of $Co_xFe_{1-x}(x {\appro}x0.62)$ metal phase in the thin films. The metal phase showed the BCC structure ($a_0$=2.89 $\AA$) and Im3m space group. Also, the cobalt ferrite phase was identified as$ CoFe_2O_4$ with a cubic structure ($a_0=8.39 $\AA$$) and a space group of Fd3m. For the higher cobalt content than the stoichiometric composition,$ Co_{37.8}Fe_{62.2}$, the thin films with high Ms and Hc could be obtained in the wide substrate temperature range (200-40$0^{\circ}C$).

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QUANTITATIVE DATA TO SHOW EFFECTS OF GEOMETRIC ERRORS AND DOSE GRADIENTS ON DOSE DIFFERENCE FOR IMRT DOSE QUALITY ASSURANCE MEASUREMENTS

  • Park, So-Yeon;Park, Jong-Min;Ye, Sung-Joon
    • Journal of Radiation Protection and Research
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    • v.36 no.4
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    • pp.183-189
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    • 2011
  • To quantitatively evaluate how setup errors in conjunction with dose gradients contribute to the error in IMRT dose quality assurance (DQA) measurements. The control group consisted of 5 DQA plans of which all individual field dose differences were less than ${\pm}5%$. On the contrary, the examination group was composed of 16 DQA plans where any individual field dose difference was larger than ${\pm}10%$ even though their total dose differences were less than ${\pm}5%$. The difference in 3D dose gradients between the two groups was estimated in a cube of $6{\times}6{\times}6\;mm^3$ centered at the verification point. Under the assumption that setup errors existed during the DQA measurements of the examination group, a three dimensional offset point inside the cube was sought out, where the individual field dose difference was minimized. The average dose gradients of the control group along the x, y, and z axes were 0.21, 0.20, and 0.15 $cGy{\cdot}mm^{-1}$, respectively, while those of the examination group were 0.64, 0.48, and 0.28 $cGy{\cdot}mm^{-1}$, respectively. All 16 plans of the examination group had their own 3D offset points in the cube. The individual field dose differences recalculated at the offset points were mostly diminished and thus the average values of total and individual field dose differences were reduced from 3.1% to 2.2% and 15.4% to 2.2%, respectively. The offset distribution turned out to be random in the 3D coordinate. This study provided the quantitative data that support the large individual field dose difference mainly stems from possible geometric errors (e.g., random setup errors) under the influence of steep dose gradients of IMRT field.

Antioxidant Effect of Mulberry Leaves and Yacon Tuber Extracts in High-fat Diet-fed Rats

  • Kim, Kwangjin;Lim, Yong;Oh, Ji Hye;Park, Un Kyu;Huh, Man Kyu;Hwang, Seock-Yeon
    • Biomedical Science Letters
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    • v.26 no.3
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    • pp.201-209
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    • 2020
  • The effect of mulberry leaves and yacon tuber extracts (MYE) on antioxidant was tested in this study. The present study investigated the in vivo effects of the anti-oxidative effect of MYE on catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione peroxidase (GSH-Px), and thiobarbituric acid reactive substances (TBARS). The seven-day acclimation of the mice was divided into six groups: Normal diet group (NOR), high fat diet group (HFD), high fat diet with 0.5% hydroxycitric acid group diet group for positive group (HHCA), high fat diet with 1% mulberry leaf and 1% yacon diet group (MYE-1), high fat diet with 3% mulberry leaf and 3% yacon group (MYE-3) and high fat diet with 5% mulberry leaf and 5% yacon group (MYE-5). The effect of serum antioxidant in the catalase of MYE-1, MYE-3, and HHCA comparing to HFD by 31.0%, 27.7% and 45.2%, respectively (P<0.05~0.01). The effect on hepatic antioxidant in the catalase of HFD was significantly increased 3.7 (77.3%) times than that of NOR (P<0.01). But, the activities of catalase were decreased significantly in MYE-1, MYE-3, MYE-5 and HHCA by 21.7%, 24.2%, 24.9%, and 28.8% compared to HFD, respectively. GSH-Px was significantly decreased in MYE-1, MYE-3, MYE-5 and HHCA by 15.5%, 37.1%, 23.4%, and 23.7% compared to HFD, respectively (P<0.05). The activities of CAT, SOD, GST, GSH-Px, and TBARS were more significantly decreased in MYE-1 and MYE-3 than those of HFD and HHCA. MYE have shown significant effects on anti-oxidative function against high fat diet.

Elimination of Harmful Heavy Metals from Sea Lettuce Enteromorpha sp. with Acid Treatment (산 처리에 의한 파래(Enteromorpha sp.)의 유해 중금속 제거)

  • Mok, Jong Soo;Son, Kwang Tae;Lee, Tae Seek;Kwon, Ji Young;Park, Kunbawui;Kim, Ji Hoe
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.1
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    • pp.1-7
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    • 2017
  • The elimination of harmful heavy metals (Cd, Cr, and Pb) from sea lettuce Enteromorpha sp. was evaluated in filtered seawater over a pH range of 2.0-4.0 using citric, hydrochloric, and nitric acids. We also evaluated the quality of sea lettuce samples after release of their internal constituents into seawater solutions containing acids. The heavy metals that accumulated in raw sea lettuce after incubation for 3 days in seawater containing Cd, Cr, and Pb were, in descending order, Pb ($120.45{\mu}g/g$), Cr ($86.04{\mu}g/g$), and Cd ($18.35{\mu}g/g$). The rate of elimination of heavy metals from sea lettuce was higher at lower pH for all of the acids used. However, the color of the sea lettuce changed adversely at below pH 2.5. The heavy metals in sea lettuce samples after 10 min in seawater at pH 3.0 containing the three acids were eliminated in the order Pb (42.2-78.0%), Cd (51.8-55.3%), and Cr (14.0-32.8%). The quality of the sea lettuce was not affected when it was incubated for 30 min at pH above 3.0. The maximum elimination of heavy metals from sea lettuce occurred when it was soaked for 10 min in seawater at pH 3.0 containing citric acid.

Analysis on Enzymatic Browning in Pine Needles (솔잎에서 발생하는 효소학적 갈변작용에 관한 분석)

  • Kong, Kwang-Hoon;Park, Hee-Joong;Choi, Sang-Sook;Cho, Sung-Hye;Kim, Yong-Tae
    • Analytical Science and Technology
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    • v.12 no.3
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    • pp.256-259
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    • 1999
  • Tyrosinases are related to the enzymatic browning of plants and attract the major scientific interest for the prevention of it. Three tyrosinase isozymes ($P_1$, $P_2$ and $P_3$) from pine needles were purified to homogeneity and characterized the factors that affect their activities. The L-ascorbic acid and ${\beta}$-mercaptoethanol notably inhibited the enzymatic activities of the three isozymes. The sodium diethyldithiocarbamate was a competitive inhibitor of isozymes with the $K_i$ values of $P_1$(0.030 mM), $P_2$(0.015 mM) and $P_3$(0.019 mM), respectively. Their enzyme activities were however, increased by the addition of most metal ions. The optimum pH for the three isozymes was 9.0~9.5 and the optimum temperatures ranged from 55 to $60^{\circ}C$ using L-DOPA as substrate.

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SENSITIVITY CALCULATIONS FOR THE COSMIC IR BACKGROUND OBSERVATIONS BY MIRIS (과학기술위성 3호 다목적 적외선 영상시스템 적외선 우주배경복사 관측 감도 계산)

  • Lee, Dae-Hui;Lee, Seong-Ho;Han, Won-Yong;Park, Jang-Hyeon;Nam, Uk-Won;Jin, Ho;Yuk, In-Su;Park, Yeong-Sik;Park, Seong-Jun;Lee, Hyeong-Mok;Park, Su-Jong;Matsumoto, Toshio;Cooray, Asantha
    • Publications of The Korean Astronomical Society
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    • v.22 no.4
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    • pp.177-181
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    • 2007
  • We present the sensitivity calculation results for observing the Cosmic Infrared Background (CIRB) by the Multi-purpose IR Imaging System (MIRIS), which will be launched in 2010 as a main payload of the Science and Technology Satellite 3 (STSAT-3). MIRIS will observe in I ($0.9{\sim}1.2um$) and H ($1.2{\sim}2.0um$) band with a $4{\times}4$ degree field of view to obtain the large scale structure (${\sim}3$ degree) of the CIRB. With the given specifications of the MIRIS, our sensitivity calculation results show that the MIRIS has a detection limit of ${\sim}9\;nW\;m^{-2}\;sr^{-1}$ (I band) and ${\sim}6\;nW\;m^{-2}\;sr^{-1}$ (H band), which is appropriate to observe the large scale structure of CIRB.