• Title/Summary/Keyword: Science laboratory

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Produce a Novel Breast Cancer Disease Model with Test-off System

  • Park, Jun-Hong;Lee, Eun-Ju;Kim, Myoung-Ok;Kim, Sung-Hyun;Park, Jung-Ok;Cho, Kyong-In;Park, Hum-Dai;Ryoo, Zae-Young
    • Proceedings of the KSAR Conference
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    • 2002.06a
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    • pp.93-93
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    • 2002
  • The utility of transgenic animal for studying the function of a particular gene in the breast system has been limited because transgenic typically occurs constitutively throughout development and in most tissue. So we use the inducible gene expression system. Several inducible gene expression system have been developed in vitro in recent years to overcome limitation with transgenic mice. (omitted)

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Evidance that Two Mouse Deafness Mutation, Cir and Sr, are Allelic

  • Cho, Kyung-In;Lee, Eun-Ju;Kim, Myoung-Ok;Kim, Sung-Hyun;Park, Jun-Hong;Park, Jung-Ok;Ryoo, Zae-Young
    • Proceedings of the KSAR Conference
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    • 2002.06a
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    • pp.68-68
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    • 2002
  • Positional cloning of hereditary deafness genes is a direct approach to identify molecules and mechanisms underlying auditory function. Nowadays many deafness genes are newly identified by finding the locus for the causative genes. Mutations at many different loci in humans and mice are known to cause hearing impairment. Mouse mutants exhibiting deafness may be useful in identifying some of genes involved. (omitted)

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Development of an Escherichia coli Biofilm Model on Transwell®

  • Kim, Bok Yung;Thyiam, General;Kang, Ji-Eun;Lee, Seung-Hwan;Park, Sang-Hee;Kim, Jung-Sun;Abraham, Marion
    • Korean Journal of Clinical Laboratory Science
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    • v.44 no.3
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    • pp.112-117
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    • 2012
  • Escherichia coli biofilm, reported to be produced in the human intestine causing a significant health risk, was successfully grown on transwell$^{(R)}$. This biofilm layer was identified by crystal violet staining and prepared for the in vitro E. coli biofilm system which can be used to screen for inhibitors. The biofilm formation did not show a change in transepithelial electrical resistance values. Furthermore, rhodamine 123 staining showed that the dye did not pass through the membrane once biofilm was formed.

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An Analysis of Laboratory Instructions in Elementary School Science (초등학교 과학 실험 수업 분석)

  • Yang, Il-Ho;Jeong, Jin-Woo;Hur, Myung;Kim, Young-Shin;Kim, Jin-Soo;Cho, Hyun-Jun;Oh, Chang-Ho
    • Journal of Korean Elementary Science Education
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    • v.25 no.3
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    • pp.281-295
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    • 2006
  • The purpose of this study was to analyze laboratory instructions used in elementary school science lessons by utilizing an analysis instrument for science laboratory instruction(AISLI). This analysis instrument was comprised of 3 elements; the aim of the laboratory activity, the interaction generated, and the inquiry process. There were also a total of 20 sub-categories and its validity was identified at 0.89 by four science educators. For the purposes of this study, 90 laboratory instructions were video-recorded, then transcribed. Laboratory instructions were analyzed by three analyst teams, and the inter-rater reliability within teams was checked through Pearson correlation, with a score of 0.91. The results of this study were as follows: the two principle aims of laboratory activity, namely, to acquire declarative knowledge and to increase attitudes toward science, were observed to be in evidence in 98.9% and 92.2% in laboratory instructions, and the levels of categories of the interaction and inquiry processes were situated at level-1. The implications of these results are that laboratory instructions currently in use do not place sufficient demands upon students' scientific thinking and are not at all conducive to student-centered activities. Therefore in order to remedy this situation, the usefulness of current science laboratory instructions need to be re-evaluated.

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Development of 80cm Telescope for Muju County Public Astronomical Observatory

  • Min, S.W.;Kim, S.J.;Jang, M.;Mun, B.S.;Lee, D.H.;Lee, T.H.;Moon, K.S.;Seol, K.H.
    • Bulletin of the Korean Space Science Society
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    • 2008.10a
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    • pp.37.1-37.1
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    • 2008
  • Space Science and Technology Laboratory at Kyung Hee University developed an 80cm Nasmyth telescope with the joint work of Space & Astronomy, Inc. It was set up at Muju county public astronomical observatory in Jeonlabuk-Do. Nasmyth focus system was selected for the telescope to use two focal points by a rotatable tertiary mirror. Focal ratios of the telescope are f10, f5 respectively. Support of the main mirror is made with Lasalle-system. This system uses 24-points in the back side of the mirror that are all resting on small counter-weights and side support is 10-points Boll link Flexible type with 2 Lasalle type. The mount is wheel & disk type Alt-Azimuth design using DC-servo motors. External high accuracy encoder has 47,600 sine-waves/rot. These encoders are used to make real-time corrections on all gearing errors.

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Effect of different concentrations of hypotaurine on melanosis and quality of Pacific white shrimp (Penaeus vannamei) during refrigeration

  • Zhou, Jiaying;Ying, Yubin;Zhou, Yaqi;Li, Gaoshang;Hu, Yaqin
    • Fisheries and Aquatic Sciences
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    • v.25 no.4
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    • pp.231-242
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    • 2022
  • Effect of different concentrations of hypotaurine (HTU) on melanosis and quality of shrimps during 10 d storage in ice were studied. During refrigeration, the total plate count and total volatile basic nitrogen of shrimps treated with 20 g/L HTU were the lowest, and the hardness and microstructure were the best. Moreover, the score of melanoses, pH and total bile acid of shrimps treated with 20 g/L HTU were also low. Sensory evaluation showed that HTU treatment could make the shelf life of shrimps 3-4 days longer compared with the control. Based on the above physical and chemical indexes, 20 g/L HTU showed great potential as a safe inhibitor in the treatment of shrimps' melanosis.