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mRNA Expression Differences of uPA, uPAR in Eutopic Endometrium of Advanced Stage Endometriosis Patients (중증 자궁내막증 환자의 자궁내막과 정상인 자궁내막에서 uPA, uPAR mRNA 발현의 차이에 관한 연구)

  • Hur, Sung Eun;Lee, Ji Young;Lee, Woon Jung;Chung, Hye-Sung;Chung, Hye Won
    • Clinical and Experimental Reproductive Medicine
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    • v.33 no.4
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    • pp.229-236
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    • 2006
  • Objective: We investigated the expression of uPA and uPAR in eutopic endometrium of advanced stage endometriosis and control patients. Methods: The 33 endometriosis patients and 32 controls were enrolled. Endometrial samples were obtained from 65 premenopausal women aged 29-44 years, undergoing laparoscopic surgery or hysterectomy for non-malignant lesions. Sufficient samples were collected from 33 patients with endometriosis stage Ill and IV and 32 controls without endometriosis confirmed by laparoscopic surgery. The mRNA expression of uPA and uPAR from eutopic endometrium were analyzed by RT-QC PCR. Results: The mRNAs of uPA and uPAR were expressed in eutopic endometrium from endometriosis and normal controls throughout the menstrual cycle. Uterine endometrium from women with endometriosis expresses significantly (p<0.05) higher levels of u-PA mRNA than endometrium from normal women without endometriosis in the proliferative phase. There were no significant differences in expression of uPAR in eutopic endometrium between controls and endometriosis patients. Conclusion: These results suggest that eutopic endometrium from endometriosis patients may be more invasive and prone to peritoneal implantation because of greater u-PA mRNA expression than endometrium from women without endometriosis. Thus, increased proteolytic activity may be one etiology for the invasive properties of the endometrium resulting in the development of endometriosis.

Mantle Ultrastructure of the Spiny Top Shell, Batillus cornutus (Gastropoda: Turbinidae) (소라(Batillus cornutus) 외투막의 미세구조)

  • Jung, Gui-Kwon;Park, Jung-Jun;Jin, Young-Guk;Ju, Sun-Mi;Lee, Jae-Woo;Jung, Ae-Jin;Lee, Jung-Sick
    • The Korean Journal of Malacology
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    • v.24 no.1
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    • pp.41-50
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    • 2008
  • The histochemical characteristics and ultrastructure of the mantle in the spiny top shell, Batillus cornutus were described using light and electron microscopy. The simple epidermal layer wrapped on the top and bottom of the centrally located connective tissue. And then the epidermal layer were divided into the outer epidermal layer near a shell and the inner epidermal layer closed to the visceral mass. The connective tissue layer was composed of the collagen fiber muscularfiber bundle and hemolymph sinus. Mucous cells in the apical mantle contained acid and neutral mucopolysaccaride, and acidic carboxylated mucopolysaccaride in the mid and marginal mantle. The mantle thickness, epidermal layer thickness and hemolymph sinus area displayed a trend of reduction from the marginal zone to the apical zone. From TEM observation, it was possible to distinguish epithelium, ciliated cell, absorptive cell and secretory cell in the epidermal layer. The epithelia were columnar and the nucleus was elliptical. The free surface were covered with microvilli. The lateral membranes of epithelium was con nected with neighboring cells by the zonular occludens, zonular adherens and membrane interdigitation. Ciliated cell on free surface had cilia and microvilli, and numerous mitochondria in the apical cytoplasm. In the epidermal layer, it observed 2 type cells having absorptive function. The absorptive cells were columnar in shape, and contained microvilli, pinocytotic vesicles, mitochondria and lysosomes of various electron density. Secretory cells can be divided into four types (A, B, C, D) depending on the cell shape and characteristics of secretory granules. These cells were unicellular glands and had similar characteristics to previously reported on the mantle of the gastropod and bivalves.

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A Study on the Characteristics Measurement of Main Engine Exhaust Emission in Training Ship HANBADA (실습선 한바다호 주기관 배기가스 배출물질 특성 고찰에 관한 연구)

  • Choi, Jung-Sik;Lee, Sang-Deuk;Kim, Seong-Yun;Lee, Kyoung-Woo;Chun, Kang-Woo;Nam, Youn-Woo;Jung, Kyun-Sik;Park, Sang-Kyun;Choi, Jae-Hyuk
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.19 no.6
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    • pp.658-665
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    • 2013
  • In this study, we measured particulate matter(PM) which emerged as the hot issue from the International Maritime Organization(IMO) and the exhaust emission using HANBADA, the training ship of Korea Maritime University. In particular, the PM was obtained with TEM grid. PM structure was observed by electron microscopy. And exhaust gases such as NOx, $CO_2$, and CO were measured using the combustion gas analyzer(PG-250A, HORIBA). The results of this study are as follows. 1) When the ship departed from the port, the maximum difference in PM emissions were up to 30 % due to the Bunker Change. 2) Under the steady navigation, emission of PM was $1.34mg/m^3$ when Bunker-A is changing L.R.F.O(3 %). And, at the fixed L.R.F.O (3 %), emission of PM was $1.19mg/m^3$. When the main engine RPM increased up to 20 % with fixed L.R.F.O(3 %), emission of PM was $1.40mg/m^3$. When we changed to low quality oil(L.R.F.O(3 %)), CO concentration from main engine increased about 16 %. On the other hand, when the main engine RPM is rising up to 20 %, CO concentration is increased more than 152 percent. These results imply that the changes of RPM is a dominant factor in exhaust emission although fuel oil type is an important factor. 3) The diameter of PM obtained with TEM grid is about $4{\sim}10{\mu}m$ and its structure shows porous aggregate.

A Study on the Nursing Needs and Satisfactions of Early Postpartum Women (초기 산욕부의 간호요구도와 만족도에 관한 연구)

  • Yoo, Youn-Ja;Lee, Eun-Ju
    • Women's Health Nursing
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    • v.5 no.3
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    • pp.389-409
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    • 1999
  • This study was carried out to identify the differences between nursing needs and levels of satisfaction of postpartum women during the early postpartum period. The goal of this study was to obtain needed to develope the nursing quality for postpartum women. The subjects were 87 postpartum women who had vaginal delivery at 2 general hospital and 2 maternity hospital in the Ulsan City. The period for the data collection was from June 1 to 29, 1999. The data was gathered using an 98 items questionnaire which was a modified version of a questionnaire the developed by Y. J. Chun. Results found are as follows : 1. The general characteristics of the subjects : The majority of subjects were 25-29 yrs. (57.5%), high school and college graduates(96.5%), unemployed(75.9%), middle ranged economics(93.1%), had no religion(43.7%), male baby(52.9%), birth weight 3-3.5Kg(49.4%), wanted pregnancy(96.6%), no abortion history(50.6%), had antenatal care(89.7%), had prenatal education(32.2%), postnatal education(42.5%), intended breast feeding (46.0%), had resonable self confidence about self(20.7%), infant care(36.8%) and nuclear family pattern(82.3%). 2. The level of nursing needs of overall nursing care(3.93) and the levels of satisfaction(3.86) was relatively high. The nursing needs by category of nursing care, the highest need was in the emotional and psychological care(4.09), and the lowest need was in physical need(3.73). The satisfactions by category of nursing care, the highest satisfaction was in emotional and psychological care(4.11) and the lowest satisfaction was in education in self care(3.64). The significant differences between nursing needs and satisfactions were found on education in infant care(p=.005), and education in self care(p=.020). 3. Among items of physical care, 'accurate medication and treatment(4.21)', 'control of postpartum hemorrhage(4.13)', 'pain control and care of episiotomy wound (4.12)' and 'regular observation of postpartum conditions(4.09)' showed high nursing needs. 'Accurate medication and treatment(4.31)', 'regular observation of postpartum condition(4.24)', 'control of postpartum hemorrhage(4.22)' and 'pain control and care of episiotomy wound(4.12)' showed high satisfaction levels. 4. Among items of emotional and psychological care, 'personal treatment(4.32)', kind and faithful care(4.30)', 'detailed explanation on the treatment or nursing care(4.25)', 'adequate draping during the care and treatment(4.23)' and detailed explanation on a doubt(4.13)', showed high nursing needs, 'personal treatment(4.52)', 'kind and faithful care(4.45)', 'detailed explanation on a doubt(4.24)', 'detailed explanation on the treatment or nursing care(4.21)' and 'adequate draping during the care and treatment(4.18)' showed high satisfaction of nursing care. Difference between the level of nursing needs and satisfaction was significant except item of 'early contacts with their baby and breast feeding'. 5. Among items of environmental care, the highest level of need and satisfaction were on the items of 'neat bedding and clothes(4.05, 4.21)' and 'room cleansing or care of room(4.01, 4.28)'. Differences between the level of nursing care and satisfaction were 'room cleansing of care of room'. 6. Among items of educational needs on self care, 'sitz bath method(4.22)', 'high risk symptoms to immediate clinic visits(4.13)', 'the timing of tub bath(4.05)' and 'good secretion of breast milk(4.03)', showed high nursing needs, 'sitz bath method(4.22)' showed high satisfaction of nursing care. Differences between the level of nursing care and satisfaction were 'the timing of hair shampoo', 'the timing of tub bath', ' the method of pad change', 'postpartum exercise', 'good secretion of breast milk', 'maintenance of breast figure', 'contraindicated drugs in postpartum women', 'kegel exercise' and 'breast self examination'. 7. Among items of educational needs on infant care, 'immunization of infants(4.36)', 'symptoms of sickness to immediate clinic visits(4.28)'. 'safety and emergency care(4.28)', 'umbilical care(4.26)', 'feeding times and intervals(4.24)', 'normal growth and development of infant(4.24)' and 'infection control(4.22)', showed high level of nursing care, 'immunization of infants(4.21)', 'feeding times and intervals(4.17)', were high satisfaction items showed significant differences between the level of nursing care and satisfaction. 8. Relationship between nursing needs and levels of satisfaction among postpartum women were as follows : 1) Physical area : There were no significant differences in the level of nursing needs, but satisfaction levels were significantly different among 'working mothers', 'baby's weights', 'baby's sex', 'planning of feeding' and 'routes of receiving postpartum informations'. 2) Emotional and psychological area : The level of nursing needs were significantly different in the area of 'confidence in self care'. Satisfaction levels were significantly different among 'baby's sex', 'baby's weights', 'the confidence of infant care' and 'working mother'. 3) Environment area : There were on significant differences in the level of nursing needs, but satisfaction levels were significantly different among 'maternal age' and 'baby's weights'. 4) Education in self care : The level of nursing needs were significantly different among 'parity history' and 'type of family formation'. Satisfaction levels were significantly different among 'working mother', 'baby's sex', 'antenatal care', 'postpartum education', 'planning of feeding', 'routes of receiving postpartum informations'. 5) Education in infant care area : The level of nursing needs were significantly different among 'parity history'. Satisfaction levels were significantly different among 'baby's sex', 'receiving postpartum education or not' and 'working mother'.

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Nutrient Recovery from Sludge Fermentation Effluent in Upflow Phosphate Crystallization Process (상향류 인 결정화공정을 이용한 슬러지 발효 유출수로 부터의 영양소 회수)

  • Ahn, Young-Ho
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.8
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    • pp.866-871
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    • 2006
  • The nutrient recovery in phosphate crystallization process was investigated by using laboratory scale uptlow reactors, adopting sequencing batch type configuration. The industrial waste lime was used as potential cation source with magnesium salt($MgCl_2$) as control. The research was focused on its successful application in a novel integrated sludge treatment process, which is comprised of a high performance fermenter followed by a crystallization reactor. In the struvite precipitation test using synthetic wastewater first, which has the similar characteristics with the real fermentation effluent, the considerable nutrient removal(about 60%) in both ammonia and phosphate was observed within $0.5{\sim}1$ hr of retention time. The results also revealed that a minor amount(<5%) of ammonia stripping naturally occurred due to the alkaline(pH 9) characteristic in feed substrate. Stripping of $CO_2$ by air did not increase the struvite precipitation rate but it led to increased ammonia removal. In the second experiment using the fermentation effluent, the optimal dosage of magnesium salt for struvite precipitation was 0.86 g Mg $g^{-1}$ P, similar to the mass ratio of the struvite. The optimal dosage of waste lime was 0.3 g $L^{-1}$, resulting in 80% of $NH_4-N$ and 41% of $PO_4-P$ removal, at about 3 hrs of retention time. In the microscopic analysis, amorphous crystals were mainly observed in the settled solids with waste lime but prism-like crystals were observed with magnesium salt. Based on mass balance analysis for an integrated sludge treatment process(fermenter followed by crystallization reactor) for full-scale application(treatment capacity Q=158,880 $m^3\;d^{-1}$), nutrient recycle loading from the crystallization reactor effluent to the main liquid stream would be significantly reduced(0.13 g N and 0.19 g P per $m^3$ of wastewater, respectively). The results of the experiment reveal therefore that the reuse of waste lime, already an industrial waste, in a nutrient recovery system has various advantages such as higher economical benefits and sustainable treatment of the industrial waste.

Assessment of the Health of Fish Species Collected in Agricultural Reservoirs, AsanHo and NamyangHo (농업용저수지 아산호와 남양호에 서식하는 어류의 건전성 평가)

  • Kim, Byung-Seok;Park, Kyung-Hun;Park, Yoen-Ki;Joeng, Mi-Hye;You, Are-Sun;Yang, Yu-Jung;Choi, Ju-Byun;Kwon, Ok-Kyung;Ahn, Young-Joon
    • The Korean Journal of Pesticide Science
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    • v.12 no.1
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    • pp.57-66
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    • 2008
  • The purpose of this study was to establish biomonitoring method for the assessment of the health of fish species in agricultural reservoirs polluted from various environmental contaminants. The ichthyofauna and fish community were investigated in the AsanHo and NamyangHo from September to November 2007. We measured abundance, size distribution, biomass, the number of fish with external and internal lesions, organosomatic index of the fishes collected in the AsanHo and NamyangHo. Dominant species were Lepomis macrochirus, Carassius auratus and the highest biomass were found in Cyprinus carpio, Carassius auratus in the AsanHo and NamyangHo, respectively. The composition of age in the population of Carassius auratus in the AsanHo and NamyangHo was stabled by size distribution analysis. The sex ratio of Carassius auratus were 1:0.36, 1:0.52 as female: male ratio in AsanHo and NamyangHo, respectively. The GSI of were 4.30, 1.69 of female and 1.45, 1.70 of male in AsanHo and NamyangHo, respectively. Most carp were 10-70 cm long in the AsanHo and 20-50 cm in the NamyangHo. The sex ratio of Cyprinus carpio were 1:0.42, 1:0.59 as female: male ratio in AsanHo and NamyangHo, respectively. The GSI of were 0.16, 3.46 of female and 0.93, 2.37 of male in AsanHo and NamyangHo, respectively. Of the 227 fish examined, 1.8% had some type of external lesion in AsanHo and 10% in NamyangHo. Overall, HAI scores ranged from 10-60 and HAI scores of AsanHo relatively lower than that of NamyangHo.

A Study on the Design of Case-based Reasoning Office Knowledge Recommender System for Office Professionals (사례기반추론을 이용한 사무지식 추천시스템)

  • Kim, Myong-Ok;Na, Jung-Ah
    • Journal of Intelligence and Information Systems
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    • v.17 no.3
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    • pp.131-146
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    • 2011
  • It is becoming more essential than ever for office professionals to become competent in information collection/gathering and problem solving in today's global business society. In particular, office professionals do not only assist simple chores but are also forced to make decisions as quickly and efficiently as possible in problematic situations that can end in either profit or loss to their company. Since office professionals rely heavily on their tacit knowledge to solve problems that arise in everyday business situations, it is truly helpful and efficient to refer to similar business cases from the past and share or reuse such previous business knowledge for better performance results. Case-based reasoning(CBR) is a problem-solving method which utilizes previous similar cases to solve problems. Through CBR, the closest case to the current business situation can be searched and retrieved from the case or knowledge base and can be referred to for a new solution. This reduces the time and resources needed and increase success probability. The main purpose of this study is to design a system called COKRS(Case-based reasoning Office Knowledge Recommender System) and develop a prototype for it. COKRS manages cases and their meta data, accepts key words from the user and searches the casebase for the most similar past case to the input keyword, and communicates with users to collect information about the quality of the case provided and continuously apply the information to update values on the similarity table. Core concepts like system architecture, definition of a case, meta database, similarity table have been introduced, and also an algorithm to retrieve all similar cases from past work history has also been proposed. In this research, a case is best defined as a work experience in office administration. However, defining a case in office administration was not an easy task in reality. We surveyed 10 office professionals in order to get an idea of how to define a case in office administration and found out that in most cases any type of office work is to be recorded digitally and/or non-digitally. Therefore, we have defined a record or document case as for COKRS. Similarity table was composed of items of the result of job analysis for office professionals conducted in a previous research. Values between items of the similarity table were initially set to those from researchers' experiences and literature review. The results of this study could also be utilized in other areas of business for knowledge sharing wherever it is necessary and beneficial to share and learn from past experiences. We expect this research to be a reference for researchers and developers who are in this area or interested in office knowledge recommendation system based on CBR. Focus group interview(FGI) was conducted with ten administrative assistants carefully selected from various areas of business. They were given a chance to try out COKRS in an actual work setting and make some suggestions for future improvement. FGI has identified the user-interface for saving and searching cases for keywords as the most positive aspect of COKRS, and has identified the most urgently needed improvement as transforming tacit knowledge and knowhow into recorded documents more efficiently. Also, the focus group has mentioned that it is essential to secure enough support, encouragement, and reward from the company and promote positive attitude and atmosphere for knowledge sharing for everybody's benefit in the company.

The Improvement Effect of Pinus densiflora Forest Disturbed by Human Trampling in the Solbat Neighborhood Park, Gangbuk-gu, Seoul (서울시 강북구 솔밭근린공원 소나무림 답압 피해 개선사업 효과 연구)

  • Kwon, Ki-Young;Han, Bong-Ho;Park, Seok-Cheol;Choi, Jin-Woo
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.5
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    • pp.148-159
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    • 2012
  • The purpose of this study is to validate the effect of improvement such measures as fence installation or planting of bush and herbaceous plants taken from Pinus densiflora forest in Solbat Neighborhood Park in Seoul, which was damaged by stamping. The study was conducted in 2005 and 2010 in order to analyze changes in planting types, planting structure of Pinus densiflora forest, soil hardness, cross-sectional structure of soil, and physicochemical characteristics of soil. It was also measured by the growth of the branches and the diameter of Pinus densiflora, comparing before and after the improvement to study the effect of restoring Pinus densiflora forest damaged by stamping. When it comes to a change in planting type, Pinus densiflora forest without underlay was reduced from 48.5% in 2005 to 6.8% in 2010. Pinus densiflora forest with bush and herbaceous plants was increased dramatically from 7.4% to 46.8%. Regarding planting structure, in most area of the subject site, Pinus densiflora forest without under layer was transformed into the one with bush and herbaceous plants including Rhododendron mucronulatum, Rhododendron schippenbachii, Hemerocallis fulva, Aceriphyllum rossii, Hosta plantaginea growing in a wide area. The soil in the Solbat Neighborhood Park was very stiff with soil hardness of $54.8kg/cm^2$ in average. After the improvement efforts made in the Park in 2010, the soil hardness was mostly less than $4kg/cm^2$, being in a good condition with little influence on the growth of plants. When it comes to the cross-sectional structure of soil, litter layer didn't exist in 2005 because of stamping and the organic matter layer was only 1.0cm thick, which provided an unfavorable condition for plant growth. However, after improvement, litter layer was formed up to 3.0cm and thickness of the organic matter layer also went up to 1.5~8.0cm in 2010 because the damage from stamping was reduced. Concerning the physicochemical characteristic of soil, in 2005 soil showed pH 5.76~6.70, organic matter content 7.15~10.55%, and available phosphorus 9.38~26.47mg/kg, having no big problems as a soil environment for growth of Pinus densiflora. 15 trees of Pinus densiflora were selected to see branch growth and it was found that the branches tended to grow better after improvement. 70 trees of Pinus densiflora from various grades of soil hardness also were selected to identify changes of diameter growth. In most cases, it was analyzed that Pinus densiflora grew better after improvement. After conducting this study, it was validated that such measures as fence installation or planting of bush and herbaceous plants to restore Pinus densiflora Forest damaged by stamping were effective in improving growth of Pinus densiflora.

The Effect of Supply Chain Dynamic Capabilities, Open Innovation and Supply Uncertainty on Supply Chain Performance (공급사슬 동적역량, 개방형 혁신, 공급 불확실성이 공급사슬 성과에 미치는 영향)

  • Lee, Sang-Yeol
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.4
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    • pp.481-491
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    • 2018
  • As the global business environment is dynamic, uncertain, and complex, supply chain management determines the performance of the supply chain in terms of the utilization of resources and capabilities of companies involved in the supply chain. Companies pursuing open innovation gain greater access to the external environment and accumulate knowledge flows and learning experiences, and may generate better business performance from dynamic capabilities. This study analyzed the effects of supply chain dynamic capabilities, open innovation, and supply uncertainty on supply chain performance. Through questionnaires on 178 companies listed on KOSDAQ, empirical results are as follows: First, integration and reactivity capabilities among supply chain dynamic capabilities have a positive effect on supply chain performance. Second, the moderating effect of open innovation showed a negative correlation in the case of information exchange, and a positive correlation in the cases of integration, cooperation and reactivity. Third, two of the 3-way interaction terms, "information exchange*open innovation*supply uncertainty" and "integration*open innovation*supply uncertainty" were statistically significant. The implications of this study are as follows: First, as the supply chain needs to achieve optimization of the whole process between supply chain components rather than individual companies, dynamic capabilities play an important role in improving performance. Second, for KOSDAQ companies featuring limited capital resources, open innovation that integrates external knowledge is valuable. In order to increase synergistic effects, it is necessary to develop dynamic capabilities accordingly. Third, since resources are constrained, managers must determine the type or level of capabilities and open innovation in accordance with supply uncertainty. Since this study has limitations in analyzing survey data, it is necessary to collect secondary data or longitudinal data. It is also necessary to further analyze the internal and external factors that have a significant impact on supply chain performance.

Effects of Acid Concentration and the Addition of Copper/Boron Salts on the Efficacy of Okara-based Wood Preservatives (두부(豆腐)비지 산(酸) 가수분해물(加水分解物)로 조제(調製)한 목재방부제(木材防腐劑)에서 산(酸) 농도(濃度)와 구리/붕소계(硼素系) 염(鹽) 첨가(添加)에 따른 방부능(防腐能)의 영향(影響))

  • Jeong, Han-Seob;Kim, Ho-Yong;Ahn, Sye-Hee;Choi, In-Gyu;Oh, Sei-Chang;Han, Gyu-Seong;Yang, In
    • Resources Recycling
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    • v.18 no.5
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    • pp.52-62
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    • 2009
  • This research was carried out to formulate environmentally friendly wood preservatives with okara and to investigate the effects of the acid concentration used for the hydrolysis of okara and salt type on the decay resistance of the preservatives. Okara-based preservatives were formulated with okara hydrolyzates, which were prepared with 0, 1%, and 2% sulfuric acid at $25^{\circ}C$ for 1 hr, and salts such as copper chloride and/or sodium borate. The preservatives were treated into wood blocks by vacuum-pressure method, and then the treated wood blocks were leached in $70^{\circ}C$ hot water for 72 hrs. The fungal treatments of the leached wood blocks were conducted by brown-rot fungus, Tyromyces palustris, and white-rot fungus, Trametes versicolor, to examine the decay resistance of the preservatives. As the acid concentration used for hydrolysis of okara increased, the treat-ability and decay resistance of the preservatives were improved, which the leachability was decreased. Wood blocks treated with the okara/copper or okara/copper/borax, showed very good decay resistance against T. palustris and T. versicolor. However, wood blocks treated with the okara/borax and okara-free preservative solutions, were observed the fungal decay by T. palustris. The optimal conditions for the preparation of okara-based wood preservatives were formulated with okara hydrolyzed with 1% sulfuric acid, copper chloride and borax.