• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.584-592
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• 2004

A thermostable $\beta$-glycosidase gene, tfi $\beta$-gly, was cloned from the genomic library of Thermus filiformis Wai33 A1. ifi $\beta$-gly consists of 1,296 bp nucleotide sequence and encodes a polypeptide of 431 amino acids. It shares a strong amino acid sequence similarity with the $\beta$-glycosidases from other Thermus spp. belonging to the glycosyl hydrolase family 1. In the present study, the enzyme was overexpressed in Escherichia coli BL21 (DE3) using the pET21b(+) vector system. The recombinant enzyme was purified to homogeneity by heat treatment and a $Ni^{2+}$-affinity chromatography. Polyacrylamide gel electrophoresis (PAGE) showed that the recombinant Tfi $\beta$-glycosidase was a monomeric form with molecular mass of 49 kDa. The temperature and pH range for optimal activity of the purified enzyme were 80- $90^{\circ}C$ and 5.0-6.0, respectively. Ninety-three percent of the enzyme activity was remained at $70^{\circ}C$ after 12 h, and its half-life at $80^{\circ}C$ was 6 h, indicating that Tfi $\beta$-glycosidase is highly thermostable. Based on its K_m$, or $K_{cat}K_m$, ratio, Tfi $\beta$-glycosidase appeared to have higher affinity for $\beta$-D-glucoside than for $\beta$-D-galactoside, however, $K_{cat} for \beta$-D-galactoside was much higher than that for $\beta$-D-glucoside. The activity for lactose hydrolysis was proportionally increased at $70^{\circ}C$ and pH 7.0 without substrate inhibition until reaching 250 mM lactose concentration. The specific activity of Tfi TEX>$\beta$-glycosidase on 138 mM lactose at $70{^\circ}C$ and pH 7.0 was 134.9 U/mg. Consequently, this newly cloned enzyme appears to have a valuable advantage of conducting biotechnological processes at elevated temperature during milk pasteurization in the production of low-lactose milk.

• Therapeutic Science for Rehabilitation
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• v.12 no.2
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• pp.55-68
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• 2023

Objective : This study aims to analyze the effects of visual perception intervention by systematically reviewing the studies that applied visual perception intervention to children with cerebral palsy. Methods : The databases used were PubMed, EMbase, Science Direct, ProQuest, Koreanstudies Information Service System (KISS), Research Information Sharing Service (RISS), and the National Assembly Library. The keywords used were cerebral palsy, CP, and visual perception. According to the PRISMA flowchart, 10 studies were selected from among studies published from January 1, 2012 to March 30, 2022. The quality level of the selected studies, the demographic characteristics of study participants, the effectiveness of interventions, area and strategies of intervention, assessment tools to measure the effectiveness of interventions, and risk of bias were analyzed. Results : All selected studies confirmed that visual perception intervention was effective in improving visual perception function. In addition, positive results were shown in upper extremity function, activities of daily living, posture control, goal achievement, and psychosocial areas as well as visual perception function. The eye-hand coordination area was intervened in all studies. Conclusion : In visual perception intervention, It is necessary to evaluate the visual perception function by area, and apply systematically graded customized interventions for each individual.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.449-456
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• 2008

Phenotypic screening for bile salt hydrolase (BSH) activity was performed on Lactobacillus acidophilus PF01 isolated from piglet feces. A gene encoding BSH was identified and cloned from the genomic library of L. acidophilus PF01. The bsh gene and surrounding regions were characterized by nucleotide sequence analysis and were found to contain a single open reading frame (ORF) of 951 nucleotides encoding a 316 amino acid protein. The potential bsh promoter region was located upstream of the start codon. The protein deduced from the complete ORF had high similarity with other BSHs, and four amino acid motifs located around the active site, FGRNXD, AGLNF, VLTNXP, and GXGXGXXGXPGD, were highly conserved. The bsh gene was cloned into the pET21b expression vector and expressed in Escherichia coli BLR(DE3) by induction with 0.1mM of isopropylthiogalactopyranoside. The BSH enzyme was purified with apparent homogeneity using a $Ni^{2+}$-NTA agarose column and characterized. The overexpressed recombinant BSH enzyme of L. acidophilus PF01 exhibited hydrolase activity against tauroconjugated bile salts, but not glycoconjugated bile salts. It showed the highest activity against taurocholic acid. The maximum BSH activity occurred at approximately $40^{\circ}C$. The enzyme maintained approximately 70% of its maximum activity even at $60^{\circ}C$, whereas its activity rapidly decreased at below $37^{\circ}C$. The optimum pH was 6, and BSH activity was rapidly inactivated below pH 5 and above pH 7.

• Journal of Korean Academy of Nursing
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• v.45 no.6
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• pp.783-801
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• 2015

Purpose: The purpose of this study is to provide a comprehensive overview of the various measures available for assessment of oxaliplatin-induced peripheral neuropathy (OXLIPN) and to evaluate the measurement properties of each assessment tool. Methods: A systematic review was conducted to identify existing measures for OXLIPN found in the databases of PubMed, Cochrane Library, Embase, RISS and KoreaMed. The quality of the 24 identified tools was evaluated based on their properties of measurement including content validity, internal consistency, criterion validity, construct validity, reproducibility, responsiveness, floor-ceiling effects and interpretability. Results: Ten (41.7%) of the 24 tools were identified as specific measures for assessing OXLIPN and the most popular type of measures were clinical grading systems by clinicians (58.3%) and only 29.2% of measures were identified as patient reported outcomes. The most frequently used tool was National Cancer Institute-Common Toxicity Criteria (NCI-CTC), but the validity of NCI-CTC has not been reported appropriately. Overall, the Neuropathic Pain Symptom Inventory (NPSI) received the best psychometric scores, and the Chemotherapy-induced Peripheral Neuropathy Assessment Tool (CIPNAT) and Functional Assessment of Cancer Therapy/Gynaecologic Oncology Group-neurotoxicity-12 (FACT/GOG-Ntx-12) followed NPSI. Conclusion: To select appropriate measure, evidences should be accumulated through the clinical use of tools. Therefore, practitioner and researchers are urged to report relevant statistics required for the validation of the currently used measures for assessment of OXLIPN.

• The Journal of Korean Obstetrics and Gynecology
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• v.33 no.3
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• pp.60-79
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• 2020

Objectives: This review plans to assess the efficacy and effectiveness of oriental medicine for the treatment of Ovarian Hyperstimulation Syndrome (OHSS) through literature research and overview. Methods: Database searching was conducted to identify relevant randomized controlled trials (RCTs) on oriental medicine for the treatment of Ovarian Hyperstimulation Syndrome. Studies were searched from Journal of Korean Obstetrics and Gynecology, Korean Medical Database, Korean studies Information Service System, China National Knowledge Infrastructure, Cochrane library, PubMed and EmBase up to 21^st May, 2020. Results: Seventeen studies were finally selected. Fifteen studies intervened with oral Chinese herb medicine, two studies intervened with acupuncture and moxibustion. Nine studies concluded that intervention with oriental medicine significantly relieved OHSS symptoms. Three studies reporting ovary diameter, four studies reporting abdominal circumference and other four studies reporting pelvic effusion showed significant reduction compared to control groups. Six studies showed significantly shorter duration for hospitalization in intervention groups. Only one study showed significantly higher pregnancy rate. Factors related with vascular permeability and blood cell coagulation were significantly lowered in intervention groups in general. Conclusions: From seventeen studies, oriental medicine relieved OHSS symptoms and showed treatment effectiveness. Further strictly designed studies and long-term observed studies are needed to establish evidences.

• Journal of Microbiology and Biotechnology
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• v.17 no.6
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• pp.891-896
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• 2007

We screened the Arabidopsis cDNA library to identify functional suppressors of AtBI-1, a gene that suppresses cell death induced by Bax gene expression in yeast. Cdf 3 encodes a 118-amino-acid protein with a molecular mass of 25 kDa. This protein has two uncharacterized domains at amino acids residues 5-64 and 74-117. In the present study, CDF3 was found to induce growth defects in yeast and arrested yeast growth, although the cell-growth defect was somewhat less than that of Bax. Its localization in the inner mitochondria was essential for suppression of yeast-cell proliferation. The morphological abnormality of the intracellular network, which is a hallmark of AtBI-1, was attenuated by Cdf3 expression.

• Journal of the Korean Society for information Management
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• v.23 no.2
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• pp.207-227
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• 2006

This study aims to examine online students' informal information seeking behavior during their loaming process and online loaming environments to support their informal information needs. The participants of the study were 29 online students in the Professional Development Studies of Rutgers University. Data was collected by the questionnaire and was analyzed with content analysis and descriptive statistics. This study focuses on when and why online students need human interaction to solve their learning problems and how they communicate with others to meet their informal information needs. Moreover, how online students think about their personal communication opportunities and the functions of their online learning system to support their loaming problems is also examined. Finally, online students suggest the ways to effectively support personal communication needed during learning process in online teaming systems. Based on the findings of this study, a few considerations are suggested in conclusions.

• The Journal of Korean Medicine
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• v.35 no.1
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• pp.1-9
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• 2014

Objectives: This study was to propose a standardized method of retention enema by extracting elements associated with published experimental methods of retention enema. Methods: 1) Literature search (1) Data sources: Retrieval was made, using 'Enema' or 'Retention Enema' as search words in Medline, Cochrane library, KISS, RISS, and KDSL DB. (2) Study selection: Two authors excluded irrelevant papers and chose qualified abstracts from the rest of the papers. The chosen studies were reviewed fully by the authors so that they could screen the significant papers based on the exclusion criteria. (3) Data extraction: Data on the total number of subjects, the target disease, the animal species, the type, quality, length, and diameter of catheter, the insertion length of catheter, the sample, the sample volume, the process of retention enema and the number of retention enemas performed were extracted from the selected studies. 2) Performing preliminary experiments about the elements associated with experimental methods of retention enema. Results & Conclusions: The standardized experimental method is as follows: animal: 280-320g SD rat 1) inserting a rectal tube of 8 Fr that measures 8 cm into the animal's anus, 2) injecting herbal medicines at the volume of 6ml and 3) holding them for 10 minutes.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.45 no.10
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• pp.75-82
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• 2008

In this paper, we propose an efficient symbol detection algorithm for multiple-input multiple-output spatial multiplexing (MIMO-SM) systems and present its design and implementation results. By enhancing the performance of the first detected symbol which causes error propagation, the proposed algorithm achieves a considerable performance gain as compared to the conventional sorted QR decomposition (SQRD) based detection and the ordered successive detection (OSD) algorithms. The bit error rate (BER) performance of the proposed detection algorithm is evaluated by the simulation. In case of 16QAM MIMO-SM system with 4 transmit and 4 receive ($4{\times}4$) antennas, at $BER=10^{-3}$ the proposed algorithm obtains the gai improvement of about 2.5-13.5 dB over the conventional algorithms. The proposed detection algorithm was designed in a hardware description language (HDL) and synthesized to gate-level circuits using 0.18um 1.8V CMOS standard cell library. The results show that the proposed algorithm can be implemented without increasing the hardware costs significantly.

• Journal of Information Management
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• v.18 no.4
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• pp.98-117
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• 1985

At the present time, the role and importance of information retrieval has greatly increased for two main reasons: the coverage of the searchable collections is now extensive and collection size may exceed several million documents; further more, the search results can now be obtained more or less instantaneously using online procedures and computer terminal devices that provide interaction and communication between system and users. The large collection size make it plausible to the users that relevant information will in fact be retrieved as a result of a search operation, and the probability of obtaining the search output without delay creates a substantial user demand for the retrieval services.