• 한국자원식물학회지
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• 제34권6호
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• pp.510-516
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• 2021

Whitening agents derived from natural sources which do not have side effects are sought after. Schizophragma hydrangeoides is an edible plant that grows wild on Jeju Island. We aimed to determine whether S. hydrangeoides extracts show anti-melanogenic activity. Here, we found that 70% ethanol extracts of S. hydrangeoides leaf suppressed α-melanocyte-stimulating hormone-induced melanogenesis in B16F10 mouse melanoma cells. This activity of anti-melanogenesis in B16F10 cells were investigated by determining melanin content and tyrosinase activity, and by performing western blotting. The 70% ethanol extract downregulated tyrosinase and tyrosinase-related protein 1. In addition, the n-hexane fraction of S. hydrangeoides leaf (HFSH) exhibited significant anti-melanogenic activity among the various solvent fractions tested without reducing the viability of B16F10 cells. Taken together, these results indicate that extracts from S. hydrangeoides leaf can influence cellular processes via modulation of tyrosinase activity. Hence, S. hydrangeoides can be used as a whitening agent in the cosmetic industry and as a therapeutic agent for treating hyperpigmentation disorders in the clinic.

• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.277-286
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• 2022

Schizophragma hydrangeoides (S. hydrangeoides) is a vine endogenous to Jeju Island and Ulleungdo, where it grows attached to the foothills and rock surfaces. Previous research has mostly focused on the whitening effect of S. hydrangeoides leaf extract. In this study, we investigated S. hydrangeoides leaf extract further, and detected four phytochemicals in the extract: chlorogenic acid, quercetin-3-O-glucosyl-(1-2)-rhamnoside, quercetin-3-O-xylosyl-(1-2)-rhamnoside, and quercitrin. We pretreated human dermal fibroblast (HDFn) cells with previously established concentrations of the four compounds for 1 h before ultraviolet A (UVA) irradiation. Among the four compounds, quercetin-3-O-glucosyl-(1-2)-rhamnoside (Q-3-GR) best inhibited matrix metalloproteinase-1 (MMP-1) levels. Thus, we investigated the protective effects of Q-3-GR on photoaging and its underlying mechanisms. Q-3-GR significantly reduced MMP-1 production and inhibited MMP-1 protein expression in UVA-irradiated HDFn cells. Furthermore, Q-3-GR increased procollagen type I production and protein expression. Q-3-GR exerted its anti-photoaging effects by downregulating the mitogen-activated protein kinase/ activator protein-1 signaling pathway, and upregulating the transforming growth factor-β/Smad signaling pathway. Thus, S. hydrangeoides leaf-derived Q-3-GR is a potential potent cosmetic ingredient for UV-induced skin aging.

• 한국환경생태학회지
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• 제26권1호
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• pp.1-10
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• 2012

본 연구는 희귀식물로 지정되어 있는 울릉도 미역고사리의 자생지 환경을 조사하여 보전 및 복원 시 기초자료를 제공하고자 하였다. 조사결과 미역고사리의 자생지는 해발고도 410~748m 범위와 경사 $12{\sim}80^{\circ}$의 암석지에 주로 생육하는 것으로 조사되었다. 식생분석결과 4개 지역의 10개 방형구내에서 조사된 관속식물은 총 66분류군이었다. 자생지 상층수목 중 교목층의 중요치는 고로쇠나무(49.52%)가 아교목층은 당마가목(28.99%)이 가장 높았으며, 관목층은 바위수국(51.99%), 섬쥐똥나무(8.82%), 너도밤나무(7.25%)가 높은 값을 보였다. 초본층은 미역고사리가 23.23%로 가장 높았으며, 다음으로는 큰두루미꽃(9.65%), 파리풀(9.23%), 관중(8.40%), 산꼬리사초(6.75%), 섬바디(5.42%) 등이 높은 값을 보여 이 종류들이 친화도가 높은 것으로 판단된다. 종다양도는 1.18로 산출되었으며, 우점도와 균등도는 각각 0.11와 0.84로 확인되었다. 토양분석 결과 토성은 사양토로 나타났으며, 포장용수량은 30.42%, 유기물함량은 17.95%, pH는 4.70으로 측정되었다. 환경특성과 식생 및 토양분석 결과에 기초한 상관분석에서는 종다양도와 종풍부도는 정의 상관관계를 보였고, 종다양도와 우점도 그리고 미역고사리의 피도와 종풍부도는 부의상관관계를 형성하였다.