• Korean Journal of Medicinal Crop Science
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• v.28 no.5
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• pp.354-359
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• 2020

Background: The recent, decline in Black-berry Magnolia Vine (Schisandra repanda; BMV) native to Jeju Island, Korea, has raised concerns about the causes of this decline. We investigated the native environment of S. repanda and evaluated its propagation through cuttings to provide preliminary data for its restoration in Jeju Island. Methods and Results: The native environment of the BMV in the Hallasan National Park was surveyed and the climatic variables (temperature and humidity) were analyzed. The effects of the sex of the donor plant (male/female) type of cutting (softwood/hardwood), and treatment with a plant growth regulator (indole-3-butylic acid/rootone) on the rooting of BMV were investigated. Additinallly, the rooting rate, root count, and root length, as well as temperature and humidity were measured in the vinyl moist chambers. BMV was observed in 63 plants distributed from 567 m to 1,364 m above sea level of the Hallasan National Park. In the cutting experiment, the rooting rate was 71.9%, and it was higer in female plants (75.0%) than in male plants (68.8%). Conclusions: The mass propagation of BMV through cuttings valuable for its restoration as without such safeguard measures, the population could face extinction within a few decades.

• Korean Journal of Medicinal Crop Science
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• v.21 no.3
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• pp.165-173
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• 2013

The fruits of Schisandra chinensis have been used as an edible ingredient and traditional medicine in Korea. Due to morphological similarities of dried mature fruits, the correct identification of S. chinensis from other closely related Schisandrae species is very difficult. Therefore, molecular biological tools based on genetic analysis are required to identify authentic Schisandrae Fructus. Random amplifed polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop an easy, reliable and reproducible method for the authentication of these four species. In this paper, we developed several RAPD-derived species specific SCAR markers and established a multiplex-PCR condition suitable to discriminate each species. These genetic markers will be useful to distinguish and authenticate Schisandrae Fructus and four medicinal plants, S. chinensis, S. sphenanthera, S. repanda and K. japonica, in species level.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.366-366
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• 2017

There are about 2 genera and 49 species of Schisandraceae all over the world which grow 2 genera and 5 species in korea. As'Schisandra'genus, S. chinensis, S. repanda, S. nigra and S. viridicarpa are distributed. As 'Kadsura' genus, there is K. japonica species. Although S. chinensis is often used for food and herbal medicine, 'Cheongsoon'is the only cultivar in korea. Therefore cultivar breeding is required for standardized production of Schisandra chinensis. To select superior lines, characteristics of collected varieties was investigated. These collections were from all the country including Mungyeong, Muju and Jangsu. In the case of 'Schisandra chinensis', analysis of chemical compound is very important. Because in 'Korean Pharmacopoeia', Schisandrin has to contain more than 0.7% of sum of Shizandrin, Gomisin A, Gomisin N as standard compound. According to the investigation, the weight of SC-154 was the highest one that was 99g of fresh fruit and 60g of dried fruit in the 100 granulas of it. As the control group, the weight of cultivar 'Cheongsoon'was 57g of fresh fruit and 40g of dried fruit. The chemical compounds such as Shizandrin A, Gomisin A, Gomisin N and Shizandrin C from the collections were analyzed by HPLC. As a result, all average of total content was 1.67% satisfied with the standard that is over 0.7%. Among them, SC-13, SC-22, SC-40 and SC-45 had more than 2.0% of compound. In this case, the content of Gomisin N was relatively higher than that of the other collections. It can be used for high content compound breeding. This above results indicate that these findings could be used for breeding superior Schisandra chinensis through selection of high quality resource.

• Korean Journal of Plant Taxonomy
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• v.46 no.3
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• pp.273-282
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• 2016

The establishment of a DNA barcode database at the regional scale and assessments of the utility of DNA barcodes are crucial for conservation biology and for the sustainable utilization of biological resources. Schisandraceae is a small family consisting of ca. 45 species. It contains many economically important species, such as Schisandra chinensis, which is widely used as a source in tonic beverages and in oriental medicine. In Korea, three species, S. chinensis, S. repanda, and Kadsura japonica, are distributed. We evaluated the level of variation of the DNA sequences of rbcL, matK, and the ITS regions from 13 accessions representing the distributional range of the three species. The three DNA barcode regions were easily amplified and sequenced. The minimum values of the interspecific genetic distances among S. chinensis, S. repanda, and K. japonica either separately or in combination are 4- to 23-fold higher than the maximum value of the intraspecific distance, showing that there is a clear DNA barcoding gap in the regions for Korean Schisandraceae. Phylogenetic analyses of the three DNA barcode regions, separately and simultaneously, indicate that all of the DNA barcode regions are useful for identifying a species of Schisandraceae in Korea. The distinctiveness of the three species of Schisandraceae was also supported at the species level when Chinese and Japanese populations were added. The results of this study indicate that three concatenated regions constitute the best option for DNA barcoding in Schisandraceae in Korea.