• Title/Summary/Keyword: Scavenging effect

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Antioxidative Activities of the Ethyl Acetate Fraction from Heated Onion (Allium cepa)

  • Lee, Youn-Ri;Hwang, In-Guk;Woo, Koan-Sik;Kim, Dae-Joong;Hong, Jin-Tae;Jeong, Heon-Sang
    • Food Science and Biotechnology
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    • v.16 no.6
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    • pp.1041-1045
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    • 2007
  • Heated onion juice was partitioned using the solvents hexane, chloroform, ethyl acetate, and butanol. The ethyl acetate fraction showed the strongest scavenging effect on the ABTS radical. The antioxidant activities of the ethyl acetate fraction from raw and heated onion (120, 130, and $140^{\circ}C$) were evaluated using radical scavenging assays. Radical and nitrite scavenging activities were higher in heated onion than raw onion, and the higher the temperature of heat treatment, the greater the radical and nitrite scavenging activities. Heated onion ($140^{\circ}C$, 2 hr) was more effective than raw onion, having higher DPPH radical scavenging (5.7-fold), hydroxyl radical scavenging (6.4-fold), superoxide radical scavenging (2.3-fold), hydrogen peroxide scavenging (11.8-fold), and nitrite scavenging (4.3-fold) activities. Onion increased its physiologically active materials after heating, and in this regard, heated onion can be used as biological material for the manufacture of health foods and supplements.

The Scavenging Effect on Nitric Oxide of Calculus Bovis.Fel Ursi.Moschus Extract Solution for Herbal-acupuncture (웅담(熊膽).우황(牛黃).사향(麝香) 약침액(藥鍼液)이 Nitric Oxide 소거(消去)에 미치는 영향(影響))

  • Youh, Eun-Joo;Kim, Jong-In;Ko, Hyung-Kyun
    • Journal of Acupuncture Research
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    • v.23 no.4
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    • pp.115-121
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    • 2006
  • Objectives : This study was designed to investigate the scavenging effect on Nitric Oxide of BUM. Methods : The scavenging effect on NO concentrations 2, 6, 12 and 24 hours after treatment with Vit C. and BUM at 1, 10, $100{\mu}g/ml$ were estimated. And those was compared with control group. Results : 1. The concentration of NO 2 hours after treatment with BUM at 1, 10, 1$100{\mu}g/ml$ were decreased compared with control group. 2. The conecntration of NO 6 hours after treatment with BUM at 1, 10, 1$100{\mu}g/ml$ were decreased compared with control group. 3. The concentration of NO 12 hours after treatment with BUM at 1, 10, $100{\mu}g/ml$ were decreased compared with control group. 4. The concentration of NO 24 hours after treatment with BUM at 1, $100{\mu}g/ml$ were decreased compared with control group. Conclusion : The scavenging effect of BUM on NO was dose-dependent.

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Experimental Study on Effects of Cucurbita moschata Duch. on Antioxidation (호박잎의 항산화 효과 연구)

  • Cha, Yun-Yeop
    • Journal of Korean Medicine for Obesity Research
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    • v.9 no.2
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    • pp.57-63
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    • 2009
  • In recent year, We are concerned about anti-aging, disease-protection, long-life, many methods are used in solving this problem. And Those are related with antioxidative ability. Recently, We heard that Cucurbita moschata Duch. has anti-hypertensive effect and good for health. So I let made a experiment for this result of the anti-oxidant effect of Cucurbita moschata Duch. used for 3 methods, those are DPPH radical scavenging activity, Nitric oxide(NO) radical scavenging activity and Bovine serum albumin(BSA). The results of this study were as follows: We measured level of DPPH radical scavenging activity. And we obtained results that the ability of DPPH radical's elimination was increased when concentration of Cucurbita moschata Duch. was to $20\;mg/m{\ell}$. We measured level of Nitric oxide(NO) radical scavenging activity. And we founded that the ability of NO radical's elimination was increased when concentration of Cucurbita moschata Duch. was to $10\;mg/m{\ell}$. When we inspected antioxidation with Bovine serum albumin(BSA). And we obtained that antioxidative ability was increased after$2.5\;mg/m{\ell}$ of Cucurbita moschata Duch.. So I guess that Cucurbita moschata Duch. has effect of antioxidative ability. Hereafter we need differential experimental methods for evidence of antioxidative effect on Cucurbita moschata Duch.

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Constituents of Antioxidative Activity and Free Radical Scavenging Effect from Galla Rhois (Rhus javanica Linne) (오배자의 항산화 활성성분 및 자유라디칼 소거효과)

  • Lee, Sung-Bae;Rhim, Tae-Jin;Lee, Kwang-Hoe;Cha, Bae-Cheon
    • Korean Journal of Pharmacognosy
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    • v.31 no.2
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    • pp.185-189
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    • 2000
  • This study was carried out to investigate the structure of antioxidative constituents and the free radical scavenging effect of the main ingredients from Galla Rhois(Rhus javanica Linne). Antioxidative activities of n-hexane, EtOAc and BuOH extracts of Galla Rhois were similar or even higher than that of natural (tocopherol) or synthetic antioxidant (BHA). It is suggested that major fractions for the antioxidative activity of Galla Rhois were the n-hexane, EtOAc and BuOH extract compartments. In the subsequently experiment, one active compound from n-hexane extract, three active compounds from EtOAc extract and one active compound from BuOH extract were isolated. Their chemical structures were identified as syringic acid, protocatechuic acid, gallic acid methylester, gallic acid and $1,2,3,4,6- penta-O-galloyl-{\beta}-D-glucose$ on the basis of the speculation of spectral data and chemical reaction. Among the compounds, protocatechuic acid, gallic acid methylester and $1,2,3,4,6- penta-O-galloyl-{\beta}-D-glucose$ showed most potent radical scavenging effect using DPPH method.

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The scavenging effect on nitric oxide (NO) in juglandis semen herbal acupunture solution (호도약침액의 Nitric Oxide (NO)에 대한 소거효과)

  • Son, Chang-Wan;Lee, Kyung-Min;Lee, Bong-Hyo;Lim, Seong-Chul;Jung, Tae-Young;Seo, Jung-Chul
    • Korean Journal of Acupuncture
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    • v.24 no.1
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    • pp.189-197
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    • 2007
  • Objectives : Free radical metabolism seems to occupy a remarkably common position in the mechanisms of aging and aging related disease. This study was designed to find out whether Juglandis Semen herbal acupuncture solution (JSHAS) can scavenge Nitric Oxide (NO) or not. Methods : SNAP was used as NO generator. NO concentration was estimated after 2, 6, 12 and 24 hrs in no treatment group, after treatment with Vitamin C or 1, 10, 100 ${\mu}g/ml$ of JSHAS. Results : There was no significant scavenging effect of JSHAS on NO after 2, 6 hrs but significant effect on NO in 10, 100 ${\mu}g/ml$ group. Conclusions : These results suggest that JSHAS has scavenging effect on NO. This study shows that JSHAS can be used for aging related disease and further studies are required to investigate the antioxidative effects of it.

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Antioxidative and Nitrite-scavenging Effects of Solvent Extracts from Gyrophora esculenta (석이버섯 용매 추출물의 항산화 및 아질산염 소거작용)

  • 정은재
    • The Korean Journal of Food And Nutrition
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    • v.11 no.4
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    • pp.426-430
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    • 1998
  • This study was conducted to investigate electron donating ability, antioxdative activity and nitrite-scavenging effect of solvent extracts from Gyrophora esculenta. The phenolic compounds of diethylether, butanol, petroleum ether and hexane extracts were 194.20$\mu\textrm{g}$/ml, 98.80$\mu\textrm{g}$/ml, 1.60$\mu\textrm{g}$/ml and 0.20$\mu\textrm{g}$/ml, respectively. Electron donating abilities of diethyleter and butanol extracts from Gyrophora esculaenta were 80.79% and 72.81%, respectively. Absorption of DPPH by diethyleter and butanol extracts sharply increaed at initial stage. Antioxidative activities of fiethylether and butanol extracts by peroxide values are rather high as compared to those of control, but low s compared to those of BHA. Nitrite-scavenging abilities of diethylether, butanol and petroleum ether from Gyrophora esculenta were 96.07%, 77.39% and 15.07%, respectively.

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Free radical Scavenging Activities and Inhibitory Effects on Xanthine oxidase of Buckwheat (Suwon No. 5) (수원 5호 메밀의 유리라디칼 소거 및 Xanthine oxidase 활성 저해)

  • 서형주;정수현;김영순;이성동
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.3
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    • pp.411-416
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    • 1997
  • To examine the free radical scavenging activities from buckwheat, Suwon No. 5 was extracted with various solvents. The seed of Suwon No. 5 had a high contents of carbohydrate, but the vegetable of that had a 31.4% of protein, 28.6% of ash and 25.7% carbohydrate as moisture free basis. Phenols contents of vegetable extracts had higher than those of seed extracts. Acetone extract of vegetable showed the greatest electron donating ability. Butanol and acetone extracts of vegetable showed high inhibitory effect on lipid peroxidation. Acetone extract of vegetable has also excellent activity in the superoxide radical scavenging activity by xanthine/xanthine oxidase-cytochrome c reduction system. The inhibitory effects of extracts on xanthine oxidase were measured. Acetone extract had the strongest inhibitory effect on xanthine oxidase and $IC_{50}$ was 2.2$\mu\textrm{g}$.

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Physiological Activity of Acai Berry (Euterpe oleracea Mart.) Extracted with Different Solvents (추출용매에 따른 아사이 베리의 생리활성)

  • Chung, Hai-Jung
    • Journal of the Korean Society of Food Culture
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    • v.27 no.1
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    • pp.75-81
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    • 2012
  • This study was conducted to investigate the physiological activity of acai berry (Euterpe oleracea Mart.) extracts from three different solvents (water, methanol, and ethanol). We measured total polyphenol and total flavonoid content, DPPH radical scavenging activity, nitrite scavenging activity, metal chelating effect, and reducing power. The extraction yield from water, methanol, and ethanol was 17.10, 9.50, and 37.51%, respectively. The highest total polyphenol content (10.54 mg/100 g) and total flavonoid contents (1.88 mg/100 g) was observed in water extract. DPPH radical scavenging activity was the highest in both water extract (72.03%) and methanol extract (74.79%) at levels of 5 mg/mL, which was similar to that of BHT (78.90%). Water extract yielded the highest metal chelating effect (92.54%) and reducing power (1.09) at levels of 5 mg/mL. Taken together, these findings suggest that extracts of acai berry can be used as functional food materials with antioxidative and nitrite scavenging activities.

Assessment of RNG Turbulence Model and the Effect of Inlet Pressure on Scavenging Flow (유니프로우 소기식 두상밸브형엔진에서 RNG 난류모델의 평가와 과급압력변화가 소기유동에 미치는 영향)

  • 조상무;허선철;박권하
    • Proceedings of the Korean Society of Marine Engineers Conference
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    • 2000.11a
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    • pp.68-81
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    • 2000
  • Many studies for improving a scavenging efficiency have been executed by using simulative manner. This paper addresses to a scavenging process in a uniflow two stroke cycle engine. The Processes are simulated for scavenging pressure variation, after the assessment of turbulent models. The results have shown that employing RNG k-$\varepsilon$ model gave more accurate velocity profiles near the cylinder wall compared with the standard k-$\varepsilon$ model and the charged fresh air has increased with the scavenging pressure increase.

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Anti-microbial Effect on Streptococcus mutans and Anti-oxidant Effect of the Butanol Fractions of Sanguisorbae Radix (지유(地楡) 부탄올 분획물의 Streptococcus mutans 항균활성 및 항산화 효과)

  • Park, Geun-Hye;Lee, Jin-Tae;An, Bong-Jeun
    • The Korea Journal of Herbology
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    • v.27 no.3
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    • pp.23-29
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    • 2012
  • Objective : Sanguisorbae Radix(SO). that belong to Rosaceae is widely distributed in Asia including Korea, Japan and China. It has been used as traditional medicine from old times as a treatment for anti-inflammatory drugs. This study was designed to investigate the effects of n-BuOH fractions of SO on anti-oxidant effect and anti-microbial effect on $Streptococcus$ $mutans$ ($S.$ $mutans$). Methods : The anti-microbial effect of $n$-BuOH fractions of SO. was assessed by the paper disk diffusion method and anti-oxidant effect was assessed by the DPPH radical scavenging effect, Superoxide anion radical scavenging effect and SOD like ability. Results : DPPH radical scavenging of the $n$-BuOH fraction from SO in 50 ug/mL was shown to be Fr-2 (89.9%), Fr-3 (88.7%), Fr-4 (76.3%), Fr-1 (59.4%), Fr-5 (56.2%). Superoxside anion radical scavenging activity of the n-BuOH fraction from SO. in 50 ug/mL for Fr-3 was shown to be 78%. SOD-like activity of n-BuOH fraction from the SO in 1000 ug/mL for Fr-3 were shown to be 76.1% respectively. The $n$-BuOH fraction from the SO had high anti-microbial effect on $S.$ $mutans$. Conclusion : As a result, the $n$-BuOH fraction from SO. has good anti-microbial and anti-oxidant effects in a concentration-dependent manner.