• Title/Summary/Keyword: Scale validation

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Optimization of an Industrial Medium and Culture Conditions for Probiotic Weissella cibaria JW15 Biomass Using the Plackett-Burman Design and Response Surface Methodology

  • Yu, Hyung-Seok;Lee, Na-Kyoung;Kim, Won-Ju;Lee, Do-Un;Kim, Jong-Ha;Paik, Hyun-Dong
    • Journal of Microbiology and Biotechnology
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    • v.32 no.5
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    • pp.630-637
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    • 2022
  • The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach. Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K2HPO4, potassium citrate, ⳑ-cysteine phosphate, MgSO4, and MnSO4) were screened by using the Plackett-Burman design. Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM). The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K2HPO4, 5.0 g/l sodium acetate, 0.1 g/l MgSO4·7H2O, 0.05 g/l MnSO4·H2O, and 1.0 g/l Tween 80. The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS). After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l). Correspondingly, JW15 showed a rapid and increased production of lactic and acetic acid in the OM. To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37℃ with or without a pH control at 6.0 ± 0.1. The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium. In conclusion, OM may be utilized for mass producing JW15 for industrial use.

A modified U-net for crack segmentation by Self-Attention-Self-Adaption neuron and random elastic deformation

  • Zhao, Jin;Hu, Fangqiao;Qiao, Weidong;Zhai, Weida;Xu, Yang;Bao, Yuequan;Li, Hui
    • Smart Structures and Systems
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    • v.29 no.1
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    • pp.1-16
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    • 2022
  • Despite recent breakthroughs in deep learning and computer vision fields, the pixel-wise identification of tiny objects in high-resolution images with complex disturbances remains challenging. This study proposes a modified U-net for tiny crack segmentation in real-world steel-box-girder bridges. The modified U-net adopts the common U-net framework and a novel Self-Attention-Self-Adaption (SASA) neuron as the fundamental computing element. The Self-Attention module applies softmax and gate operations to obtain the attention vector. It enables the neuron to focus on the most significant receptive fields when processing large-scale feature maps. The Self-Adaption module consists of a multiplayer perceptron subnet and achieves deeper feature extraction inside a single neuron. For data augmentation, a grid-based crack random elastic deformation (CRED) algorithm is designed to enrich the diversities and irregular shapes of distributed cracks. Grid-based uniform control nodes are first set on both input images and binary labels, random offsets are then employed on these control nodes, and bilinear interpolation is performed for the rest pixels. The proposed SASA neuron and CRED algorithm are simultaneously deployed to train the modified U-net. 200 raw images with a high resolution of 4928 × 3264 are collected, 160 for training and the rest 40 for the test. 512 × 512 patches are generated from the original images by a sliding window with an overlap of 256 as inputs. Results show that the average IoU between the recognized and ground-truth cracks reaches 0.409, which is 29.8% higher than the regular U-net. A five-fold cross-validation study is performed to verify that the proposed method is robust to different training and test images. Ablation experiments further demonstrate the effectiveness of the proposed SASA neuron and CRED algorithm. Promotions of the average IoU individually utilizing the SASA and CRED module add up to the final promotion of the full model, indicating that the SASA and CRED modules contribute to the different stages of model and data in the training process.

The Validity Study of the CAPP-SR in Korean Correctional Offenders (자기보고식 사이코패시 성격 종합평가 도구(CAPP-SR) 타당화 연구: 한국 수형자 집단을 중심으로)

  • Sea, Jonghan
    • Korean Journal of Forensic Psychology
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    • v.12 no.3
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    • pp.301-321
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    • 2021
  • The Comprehensive Assessment of Psychopathic Personality(CAPP) is an emerging comprehensive approach that makes use of 99 items in 33 symptoms to well-represented psychopathic personality disorder, but cross-cultural generalizability of this potential model have not endured extensive validation to date. The current study sought to validate the recently published CAPP-Self-Report form(CAPP-SR). 311 participants were derived from eleven prisons by the systematic sampling. They were administered the CAPP-SR, Triarchic Psychopathy Measure(TriPM), Levenson's SRP(LSRP), Short Dark Tetrad(SD-4), and Korean Offender Risk Assessment-General(KORAS-G designed for predicting Korean offenders'recidivism. The results showed that the CAPP-SR total, domain, and symptom scores were generally associated with TriPM, LSRP, SD-4, and KORAS-G scores in a manner consistent with conceptual expectations. Also. the findings cross-culturally provide support for construct validity and incremental validity of the CAPP-SR in Korea as well as have clinical implications for the CAPP model more broadly, which are discussed.

Study on Radionuclide Migration Modelling for a Single Fracture in Geologic Medium : Characteristics of Hydrodynamic Dispersion Diffusion Model and Channeling Dispersion Diffusion Model (단일균열 핵종이동모델에 관한 연구 -수리분산확산모델과 국부통로확산모델의 특성-)

  • Keum, D.K.;Cho, W.J.;Hahn, P.S.;Park, H.H.
    • Nuclear Engineering and Technology
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    • v.26 no.3
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    • pp.401-410
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    • 1994
  • Validation study of two radionuclide migration models for single fracture developed in geologic medium the hydrodynamic dispersion diffusion model(HDDM) and the channeling dispersion diffusion model(CDDM), was studied by migration experiment of tracers through an artificial granite fracture on the labolatory scale. The tracers used were Uranine and Sodium lignosulfonate know as nonsorbing material. The flow rate ranged 0.4 to 1.5 cc/min. Related parameters for the models were estimated by optimization technique. Theoretical breakthrough curves with experimental data were compared. In the experiment, it was deduced that the surface sorption for both tracers did not play an important role while the diffusion of Uranine into the rock matrix turned out to be an important mass transfer mechanism. The parameter characterizing the rock matrix diffusion of each model agreed well The simulated result showed that the amount of flow rate could not tell the CDDM from the HDDM quantitatively. On the other hand, the variation of fracture length gave influence on the two models in a different degree. The dispersivity of breakthrough curve of the CDDM was more amplified than that of the CDDM when the fracture length was increased. A good agreement between the models and experimental data gave a confirmation that both models were very useful in predicting the migration system through a single fracture.

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A Delphi Study to Validate the Patient-Centered Doctor's Competency Framework in Korea (한국의 환자 중심 의사 역량 프레임 타당화를 위한 델파이 연구 )

  • Sunju Im;Young-Jon Kim;Chanwoong Kim;Geon-Ho Lee;Sun-Woo Lee;Woo-Taek Jeon;Hanna Jung;Sojung Yune
    • Korean Medical Education Review
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    • v.25 no.2
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    • pp.139-158
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    • 2023
  • Defining a competent doctor is important for educating and training doctors. However, competency frameworks have rarely been validated during the process of their development in Korea. The purpose of this study was to validate the patient-centered doctor's competency framework, which had been developed by our expert working group (EWG). Two rounds of Delphi questionnaire surveys were conducted among a panel of experts on medicine and medical education. The panel members were provided with six core competencies, 17 sub-competencies, and 53 enabling competencies, and were asked to rate the importance of these competencies on a 5-point Likert scale. Between April and July 2021, a total of 28 experts completed both rounds. The data of the Delphi study were analyzed for the mean, standard deviation, median, inter-rater agreement (IRA), and content validity ratio (CVR). A CVR >0.36 and IRA ≥0.75 were deemed to indicate validity and agreement. This study found that five enabling competencies were not valid, and agreement was not reached for three sub-competencies and two enabling competencies. In consideration of CVR and the individual opinions of panel members at each session, the final competencies were extracted through consensus meetings of the EWG. The competencies were modified into six core competencies, 16 sub-competencies, and 47 enabling competencies. This study is meaningful in that it proposes patient-centered doctor's competencies enabling the development of residents' milestone competencies, an assessment system, and educational programs.

The Effects of Franchise Firm's Reputation on Trust and Loyalty (외식프랜차이즈 기업의 평판이 신뢰와 충성도에 미치는 영향)

  • Kim, Hye-Rim;Han, Young-Wee;Cho, Hye-Duck
    • The Korean Journal of Franchise Management
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    • v.8 no.2
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    • pp.37-47
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    • 2017
  • Purpose - Recently, the food service franchise market is experiencing rapid growth and competition is intensifying. Therefore, consumer choice has expanded, and reputation management has become important as a strategy for survival of corporations. Based on previous studies, this research proposed the theoretical framework about the structural relationships among reputation, trust(cognitive trust, affective trust), and loyalty. Research design, data, and methodology - This study examined the structural relationship between reputation, trust, and loyalty from the customer's perspective. Based on comprehensive validation procedures across nine food service Franchise firm types, This study found support for a five-dimensional scale with the following dimensions: Customer Orientation, Employer Brand, Reliable and Financially Strong Company, Product and Service Quality, and Social and Environmental Responsibility. In order to verify the research purposes, research model and hypotheses were developed. The data were collected from 227 food service franchise consumers through online survey. The data was analyzed with SPSS 24.0 and Amos 23.0 statistical program. Result - The results of the study are as follows. First, customer orientation, reliable·financially strong company and product·service quality have significant impact on corporate cognitive trust. And employer brand, product/service quality and social·environmental responsibility have significant impact on corporate affective trust. Second, cognitive trust and affective trust have significant impacts on consumer loyalty. Conclusions - The implications of this study are following as: From the theoretical perspective, this study considers trust as two dimensions such as cognitive and affective, not a single dimension, and identify what dimensions of franchise firms affect consumers' reputation perception and in turn lead cognitive and affective trust, and loyalty. This study also provides several managerial implications. In the franchise market where competition is intensifying, it is very important to analyze the attitudes of consumers in order to gain an advantage in competition with other competitors. In this study, it is meaningful that the study was conducted on consumers who have experience using a restaurant franchise company. Also, reputation is necessary to pay attention to the company because it is an important variable that strengthens with customer through confidence in food service franchise business, and leads loyalty and consumer consumption. Therefore, marketers should develop marketing strategies considering various reputation factors.

Development and Application of Practical Ability Test for Pre-service Science Teacher (Female) (여성예비과학교사에 대한 교직수행능력검사도구의 개발과 적용)

  • Jang, Jyung-Eun;Kim, Sung-Won
    • Journal of The Korean Association For Science Education
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    • v.29 no.1
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    • pp.43-53
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    • 2009
  • The teacher's role in education is important. Science education majors must be able to solve problems effectively and pertinently when facing new ones in various situations and complicated human relations in order to become successful science teacher. The purpose of this research is to develop a test that measures the Practical Ability of pre-service science teachers and to apply this to them. The Practical Efficacy Scale for Science Education Majors was also developed in order to be used for validation. In this research, Practical Ability of Science Education Majors consisted of four sub-domains: subject education, business administration, relations and self-development. The result of the correlations between the scores of four sub-domains and the composite score of Practical Ability Test for Preservice Science Teacher(PATPST) is relevant. Subject education and administration business is the highest correlation with PATPSP score specially, and correlation between two areas appeared high. The result of applying PATPSP scores differed according to the grade of science education majors, but not according to their majors. This study's limitation is that the subjects consisted only of female students. However, PATPSP could be a new method that will help science education majors be successful science teachers.

Evaluation of the CNESTEN's TRIGA Mark II research reactor physical parameters with TRIPOLI-4® and MCNP

  • H. Ghninou;A. Gruel;A. Lyoussi;C. Reynard-Carette;C. El Younoussi;B. El Bakkari;Y. Boulaich
    • Nuclear Engineering and Technology
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    • v.55 no.12
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    • pp.4447-4464
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    • 2023
  • This paper focuses on the development of a new computational model of the CNESTEN's TRIGA Mark II research reactor using the 3D continuous energy Monte-Carlo code TRIPOLI-4 (T4). This new model was developed to assess neutronic simulations and determine quantities of interest such as kinetic parameters of the reactor, control rods worth, power peaking factors and neutron flux distributions. This model is also a key tool used to accurately design new experiments in the TRIGA reactor, to analyze these experiments and to carry out sensitivity and uncertainty studies. The geometry and materials data, as part of the MCNP reference model, were used to build the T4 model. In this regard, the differences between the two models are mainly due to mathematical approaches of both codes. Indeed, the study presented in this article is divided into two parts: the first part deals with the development and the validation of the T4 model. The results obtained with the T4 model were compared to the existing MCNP reference model and to the experimental results from the Final Safety Analysis Report (FSAR). Different core configurations were investigated via simulations to test the computational model reliability in predicting the physical parameters of the reactor. As a fairly good agreement among the results was deduced, it seems reasonable to assume that the T4 model can accurately reproduce the MCNP calculated values. The second part of this study is devoted to the sensitivity and uncertainty (S/U) studies that were carried out to quantify the nuclear data uncertainty in the multiplication factor keff. For that purpose, the T4 model was used to calculate the sensitivity profiles of the keff to the nuclear data. The integrated-sensitivities were compared to the results obtained from the previous works that were carried out with MCNP and SCALE-6.2 simulation tools and differences of less than 5% were obtained for most of these quantities except for the C-graphite sensitivities. Moreover, the nuclear data uncertainties in the keff were derived using the COMAC-V2.1 covariance matrices library and the calculated sensitivities. The results have shown that the total nuclear data uncertainty in the keff is around 585 pcm using the COMAC-V2.1. This study also demonstrates that the contribution of zirconium isotopes to the nuclear data uncertainty in the keff is not negligible and should be taken into account when performing S/U analysis.

Characteristics of Sleep Patterns in Korean Women Golfers (여자 골프선수들의 수면양상조사)

  • Park, Soo Yeon;Shin, Won-Chul
    • Sleep Medicine and Psychophysiology
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    • v.21 no.2
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    • pp.80-84
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    • 2014
  • Introduction: Sleep has numerous important physiological and cognitive functions that may be particularly important to elite athletes. Sleep deprivation can have significant effects on athletic performance. However, there are few published data related to the amount of sleep obtained by elite athletes. We investigated sleep patterns of Korean women golfers using sleep-related questionnaires. Methods: For this study, 98 Korean university women golfers and 46 age- and sex-matched controls were recruited. All subjects were asked to complete the self-administered sleep questionnaire consisting of questions about habitual sleep patterns (sleep onset time, sleep latency, awakening time in the morning, day time napping time), exercise habits, Epworth Sleepiness Scale (ESS), Insomnia Severity Index (ISS), Pittsburgh Sleep Quality Index (PSQI), validation of the Perceived Stress Scale (PSS), and Beck Anxiety Inventory (BAI). Results: The sleep onset time was significantly earlier (pm 23 : $05{\pm}00$ : 52 and 00 : $14{\pm}00$ : 51 ; t = 5.287, p < 0.001), the waking time was later (am 07 : $21{\pm}01$ : 09 and 6 : $35{\pm}00$ : 32; t = -2.715, p = 0.008), the weekday total sleep time was greater ($417.77{\pm}78.18$ minute and $351.52{\pm}77.83$ minute ; t = 4.406, p = 0.001), and the daytime nap time was greater ($77.73{\pm}41.28$ minute and $20.22{\pm}33.03$ minute ; t = 7.623, p < 0.001) in the golf athletes compared to the controls. The PSQI scores were significantly lower, but estimated sleep latency and ESS, ISS, PSS, and BAI scores were not different among the two groups. Conclusion: This study suggests that Korean university women golfers have good sleep patterns resulting in no difference in sleep-related stress compared to age- and sex-matched control students.

Comparison of Multi-Satellite Sea Surface Temperatures and In-situ Temperatures from Ieodo Ocean Research Station (이어도 해양과학기지 관측 수온과 위성 해수면온도 합성장 자료와의 비교)

  • Woo, Hye-Jin;Park, Kyung-Ae;Choi, Do-Young;Byun, Do-Seung;Jeong, Kwang-Yeong;Lee, Eun-Il
    • Journal of the Korean earth science society
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    • v.40 no.6
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    • pp.613-623
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    • 2019
  • Over the past decades, daily sea surface temperature (SST) composite data have been produced using periodically and extensively observed satellite SST data, and have been used for a variety of purposes, including climate change monitoring and oceanic and atmospheric forecasting. In this study, we evaluated the accuracy and analyzed the error characteristic of the SST composite data in the sea around the Korean Peninsula for optimal utilization in the regional seas. We evaluated the four types of multi-satellite SST composite data including OSTIA (Operational Sea Surface Temperature and Sea Ice Analysis), OISST (Optimum Interpolation Sea Surface Temperature), CMC (Canadian Meteorological Centre) SST, and MURSST (Multi-scale Ultra-high Resolution Sea Surface Temperature) collected from January 2016 to December 2016 by using in-situ temperature data measured from the Ieodo Ocean Research Station (IORS). Each SST composite data showed biases of the minimum of 0.12℃ (OISST) and the maximum of 0.55℃ (MURSST) and root mean square errors (RMSE) of the minimum of 0.77℃ (CMC SST) and the maximum of 0.96℃ (MURSST) for the in-situ temperature measurements from the IORS. Inter-comparison between the SST composite fields exhibited biases of -0.38-0.38℃ and RMSE of 0.55-0.82℃. The OSTIA and CMC SST data showed the smallest error while the OISST and MURSST data showed the most obvious error. The results of comparing time series by extracting the SST data at the closest point to the IORS showed that there was an apparent seasonal variation not only in the in-situ temperature from the IORS but also in all the SST composite data. In spring, however, SST composite data tended to be overestimated compared to the in-situ temperature observed from the IORS.