• Korean Journal of Microbiology
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• v.47 no.2
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• pp.167-169
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• 2011

The Saccharomyces cerevisiae S288c strain does not show haploid and diploid filamentous growth, and biofilm formation, because it has a flo8 nonsense mutation unlike ${\Sigma}1278b$ strain which has a FLO8 gene. During the heat stress experiments to investigate the role of ScKns1, LAMMER kinase in S. cerevisiae, we found that ${\Sigma}1278b$ strain revealed heat sensitivity at $37^{\circ}C$, a mild heat stress in contrast to S288c strain. We also found that the disruption of ScKns1 and the addition of sorbitol suppress heat sensitivity of ${\Sigma}1278b$ strain. These results suggest the possibility that Flo8 and ScKns1 may interact to transducer a signal for regulating heat stress through a novel signaling pathway.

• Korean Journal of Microbiology
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• v.47 no.2
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• pp.110-116
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• 2011

In the previous study with the Saccharomyces cerevisiae S288c strains, no known function of the dual specificity kinase, ScKns1, was reported because its gene deletion did not show any noticeable phenotypic changes. Recent study with fission yeast, however, revealed the involvement of the LAMMER kinase in flocculation, filamentous growth, oxidative stress, and so on. Therefore we made Sckns1-deletion mutants with the ${\Sigma}1278b$-background, with which one can induce filamentous and adhesive growth in contrast to those of the S288c-background. The $Sckns1{\Delta}$ strains of both haploid and diploid showed defect in filamentous growth under conditions for inducing the filamentous growth such as nitrogen starvation and butanol treatment. Both kinds of the deletion mutants also showed decrease in adhesive growth on agar surface. Interestingly enough the defects of the $Sckns1{\Delta}$ strains were suppressed by the over-expression of each gene for the components of the MAPK signaling pathway such as STE11, STE12, and TEC1, respectively, but not by the upstream components, RAS2 and STE20, respectively. Although further investigations are required, these results indicate that the ScKns1 may act in place between the Ste20 and the Ste11 of the S. cerevisiae MAPK cascade.