• Journal of Mushroom
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• v.10 no.1
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• pp.21-28
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• 2012

Studies were made to optimize the media composition and cultural condition for mycelial growth of Agrocybe cylindracea. Sawdust spawn of media composition for optimal growth was found to be pine sawdust combination of 30% wheat bran and poplar sawdust combination of 20% corn bran were the best of the optimal combination. The optimal concentration of white sugar was 1.0~1.5%. The nitrogen sources was found to be yeast extract and soybean powder. Also, optimal concentration were $0.7g/{\ell}$ and $0.1g/{\ell}$, respectively. The mineral sources of optimal medium compositions were $MgSO_4{\cdot}7H_2O\;0.3g/{\ell}$, $KH_2PO_4\;0.5g/{\ell}$ and $K_2HPO\;1.2g/{\ell}$. Optimal amount of inoculum for cultivation of A. cylindracea were $20{\sim}25g/850m{\ell}$ and $25m{\ell}/850m{\ell}$ in the sawdust spawn and liquid spawn, respectively.

• The Korean Journal of Mycology
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• v.51 no.2
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• pp.69-80
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• 2023

The cultivation conditions of shiitake mushrooms (Lentinula edodes) influence the production and quality of fruiting bodies. We conducted this study to improve the productivity and quality of shiitake mushrooms by modifying the cultivation conditions. Two types of spawns (sawdust and liquid spawn) were used, and corn flour was used as a nutritional source for the sawdust medium. A blue light-emitting diode (LED; 300 lux) was also used instead of a white LED during the incubation period. Sanbaekhyang was used as the experimental variety. When using corn flour, the mycelial growth rate increased 1.1 to 2.7 times the growth rate of the control up to 21 days of incubation, and the weight loss rate of the media was also higher. Mushroom productivity increased 1.2 times when the liquid spawn was used compared to when the sawdust spawn was used, and the blue LED also increased fruiting body production by 1.1 times compared to the white LED. Mushroom productivity increased when the liquid spawn was used, and the blue LED also increased fruiting body production. Fruiting body weight and the size of the cap were greater when sawdust spawn was used. The fruiting body weight and the stipe diameter were greater when the blue LED was used. Taste analysis showed that the saltiness increased when corn flour was used, and the sourness increased when the blue LED was used.

• Journal of Mushroom
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• v.12 no.3
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• pp.216-219
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• 2014

Studies were made to optimize the media composition in bag culture and conducted to determine the possibility of artificial cultivation of Auricularia auricula. Sawdust spawn of media composition for optimal growth were found to be oak-sawdust 80%combination of 20% popla-sawdust were the best of the optimal combination. And optimal substrate combination were found to be cotton-seed meal combination of 10% wheat bran 5% mixed were the best of combination. The duration of spawn run period and primordial formation period on bag(1.2 kg) were 50 days and 7 days, respectively. The weight of fruiting body and the yield(100ea, 1.2 kg) of fresh fruit-body were 24 g and 45,000 g, respectively.

• Journal of Mushroom
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• v.15 no.3
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• pp.104-110
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• 2017

In order to determine the possibility of artificial cultivation of Ganoderma lucidum, a study was carried out to optimize the medium composition used for bed culture. Sawdust spawn of medium composition for optimal growth was found to be the combination of 75% oak-sawdust, 10% cotton-seed meal, and 15% beet pulp. The optimal conditions for the formation of fruiting bodies were shown to be a temperature of $28-30^{\circ}C$, light intensity of 1,000-1,500 lux, and $CO_2$ concentration of 1%.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.6 no.3
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• pp.255-260
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• 2005

PAHs are aromatic hydrocarbon compunds with two or more benzene rings. Because they are mostly toxic to human life, they need to be converted to non-toxic compunds or to be degraded completely. This work aims to degrade PAHs (phenanthrene and pyrene) using Bacillus bacteria covered on cork or sawdust. The results show that media effect on phenanthrene was negligible whereas biodegradation ability of sawdust carrying the bacteria was better than that of biofilm-covered cork when pyrene was tested. PAH removal was also affected by soil moisture content with $45\~55\%$ of the optimal content.

• The Korean Journal of Mycology
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• v.46 no.4
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• pp.495-503
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• 2018

Oak mushroom is cultivated using logs and sawdust media as substrates. In this study, fungi were isolated during a monitoring of indoor air in the oak mushroom cultivation houses located in Cheongyang-gun of Chungnam, Geoje-gun of Gyeongnam, Gumi-si of Gyeongbuk, Jangheung-gun of Jeonnam and Yeoju-si of Gyeongggi-do. Identification of the fungi based on morphology and molecular analysis of the internal transcribed spacer region and 28S rDNA, translation elongation factor translation elongation factor 1 a gene, and ${\beta}-tubulin$ gene revealed that six fungi, Cenangium acuum, Neopestalotiopsis surinamensis, Metarhizium marquandii, Periconia macrospinosa, Trichoderma petersenii, and Trichoderma paratroviride that have not been recorded previously in Korea.

• Mycobiology
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• v.29 no.3
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• pp.160-163
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• 2001

Sikhae is a Korean traditional beverage of saccharified rice. Its factory waste(SFW) is usually thrown away instead of being used. We developed a cheap substrate of SFW for use in liquid spawn that is known for its higher fruit body yields than grain spawn in sawdust cultivation. Mycelia of Lentinula edodes ASI 3046, which is regarded as the most suitable strain for sawdust cultivation, were cultured on six kinds of previous known media and SFW. As the seven kinds of media were applied, a Sikhae Factory Waste(SFW) was most excellent in growth. The dried mycelial weight in SFW was almost four times as much as that in the other media. In the flask culture, optimum culture conditions for the mycelial growth were obtained after 13 days of cultivation at media volume of 100 ml, 100 rpm, initial pH 4.5, and $25^{\circ}C$. The best mycelial growth was observed when $MgSO_4{\cdot}7H_2O$ and D-sucrose were added as a supplement in SFW. SWM must be a remarkable medium for L. edodes because of its simple preparation and low cost.

• Journal of Mushroom
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• v.7 no.4
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• pp.156-162
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• 2009

These experiments were carried out to determine the optimal culture conditions for nine strains of collected Hatakeshimeji, Lyophyllum decastes (Fr.:Fr.) Sing. SPA 202 and SPA 205 strains were selected because mycelium grew fast and showed fine density. All strains showed fast mycelial growth and mycelial density on BC(Burke compost) media for 20 days of incubation. The optimal sawdust species for the mycelial growth were the fermented sawdusts of Quercus aliena and Populus deltoides. Spawn running period on the fermented sawdust substrate required 50 days at 20 to $25^{\circ}C$ and additional 7 days after soil casing. Cultivation period and temperature forprimordia formation and fruitbody development appeared from 10 to 11 days and from 7 to 8 days at 17 to $18^{\circ}C$ respectively. The length of pilei and stipes of SPA 202 harvested in optimal stage showed 60mm and 67mm, respectively. Yield of SPA 202 strain grown on fermented sawdust substrate was 130g per 1,100ml in bottle cultivation. The length of pilei and stipes of SPA 205harvested in optimal stage showed 51mm and 81mm, respectively. Yield of SPA 205 strain grown on fermented sawdust substrate was 129g per 1,100 ml in bottle cultivation. SPA 202 strain and SPA 205 strain in artificial bottle cultivation of Lyophyllum decastes used in fermented sawdust substrate were selected as themost appropriate strain in yield.

• Microbiology and Biotechnology Letters
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• v.7 no.1
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• pp.37-46
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• 1979

In order to investigate the compositional change of the lauan and pine sawdust media during the coltivation of oyster mushroom (Pleurotus ostreatus) chemical components of the two media and mushroom were analized periodically from spawning to ending. The results are summerized as follows: 1) The total yield of mushroom from the lauan sawdust media was 6.39 kg/$m^2$, and from the Pine saw-dust media 6.31 kg/$m^2$, 94％ of which was produced from the first and second cropping period. 2) In both media ash content increased gradually, while organic matter content decreased as the cultivation progressed. Moisture content, which was about 79％ at the time of spawning, decreased a little during the priod of mycelial propagation, after which no change was observed. 3) Total nitrogen content of the two media decreased gradually as the cultivation progressed, and total loss of insoluble nitrogen was greater than that of soluble nitrogen. Amino nitrogen content tended to increase continually. 4) In both media pentosan, $\alpha$-cellulose, lignin and C/N ratio decreased gradually, while the content of reduced sugar, trehalose and mannitol continued to increase. 5) In both media phosphorus, potassium, manganese and zinc decreased, and magnecium, calcium, iron and copper showed irregular changes. 6) CMC-saccharifying and liquefying activity gradually increased from after mycelial propagation to the second cropping, after which it decreased in both media. Xylanase activity rapidly and greatly increased during the second cropping period rather than the first period. At the start of the third cropping period the activity decreased slowly. Protease activity was highest after mycelial propagati, after which it gradually decreased. The pH of the two media decreased as the cultivation progressed. 7) In both media little change was observed in the content of the components of mushroom cropped from the first to the third period, but slight decrease was noticed at the fourth cropping.

• Journal of Bio-Environment Control
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• v.9 no.1
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• pp.47-59
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• 2000

In order to investigate the physical and chemical properties of artificial culture media, the specimens were substituted with 5~20％ clay, 10~30％(w) quick lime, 5~l5％(w) burnt plaster and 10％(w) sawdust. Fly ash-clay bodies were sintered at 1,050~1,20$0^{\circ}C$ and then their properties were determined. It was found that 90FA10JC(fly ash ＋clay(90：10, ％(w)) specimen sintered at 1,15$0^{\circ}C$ for 10 min. had good physical and chemical properties. When this composition was supplement with 10％(w) sawdust, bulk density water absorption, apparent porosity, compressive strength and pH after 240 hrs curing time were 1.14, 54.4％, 39.5％, 54 kgf.cm$^{-2}$ and 7.1 respectively. The physical properties of fly ash-quick lime-burnt plaster system specimens were superior to FAJC systems. However, this composition we not suitable as a artificial culture media because of its high pH. In this study, it was shown that 90FA10JC10SD(90FA10JC ＋10％(w) sawdust) system exhibited the best physical properties.