• Fibers and Polymers
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• v.6 no.3
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• pp.181-185
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• 2005

Silk fibroin (SF) nanofibers were prepared by electrospinning and their application as an enzyme immobilization support was attempted. By varying the concentration of SF dope solution the diameter of SF nanofiber was controlled. The SF nanofiber web had high capacity of enzyme loading, which reached to $5.6\;wt\%$. The activity of immobilized a-chymotrypsin (CT) on SF nanofiber was 8 times higher than that on silk fiber and it increased as the fiber diameter decreased. Sample SF8 (ca. 205 nm fiber diameter) has excellent stability at $25^{\circ}C$ by retaining more than $90\%$ of initial activity after 24 hours, while sample SF11 (ca. 320 nm fiber diameter) shows higher stability in ethanol, retaining more than $45\%$ of initial activity. The formation of multipoint attachment between enzyme and support might increase the stability of enzyme. From these results, it is expected that the electrospun SF nanofibers can be used as an excellent support for enzyme immobilization.

• Journal of Korean Academy of Nursing
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• v.43 no.5
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• pp.587-594
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• 2013

Purpose: The purpose of this study is to provide researchers with a simplified approach to undertaking exploratory factor analysis for the assessment of construct validity. Methods: All articles published in 2010, 2011, and 2012 in Journal of Korean Academy of Nursing were reviewed and other relevant books and articles were chosen for the review. Results: In this paper, the following were discussed: preliminary analysis process of exploratory factor analysis to examine the sample size, distribution of measured variables, correlation coefficient, and results of KMO measure and Bartlett's test of sphericity. In addition, other areas to be considered in using factor analysis are discussed, including determination of the number of factors, the choice of rotation method or extraction method of the factor structure, and the interpretation of the factor loadings and explained variance. Conclusion: Content validity is the degree to which elements of an assessment instrument are relevant to and representative of the targeted construct for a particular assessment purpose. This measurement is difficult and challenging and takes a lot of time. Factor analysis is considered one of the strongest approaches to establishing construct validity and is the most commonly used method for establishing construct validity measured by an instrument.

• Journal of Korean Society for Atmospheric Environment
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• v.13 no.3
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• pp.207-214
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• 1997

We collected airborne complex mixtures in a industrial area of Yeochun, and examined whether these complex mixtures could affect gap junctional intercellular communication (GJIC) in a cultured WBF-344 rat liver epithelial cells (LEC). Since the reduction of GJIC plays an important role in chemical carcinogenesis, measurement of changes of GJIC is a meaningful method to screen carcinogenicity of these mixtures. High and low volume samples were dissolved in dimethyl sulfoxide (DMSO) and tested. Blank filter extractions were also examined for exclud-ing possible toxicity of filter itself, and TPA (12-O-tetradecanoylphorbol-13-acetate) and DMSO were used as positive and negative control, respectively. When the cells were exposed to samples at concentration below that required to maintain rather than 85% cell viability based on the result of neutral red uptake assay, maximal inhibition of GJIC was observed at 1hr after treatment with both high and low volume samples by scrape-loading dye transfer assay. In fluorescence recovery after photobleaching assay, recovery rates via gap junctions were 33%/min in high volume sample and 62%/min in low volume sample. In together, airborne samples collected in Yeochun inhibited GJIC in a cultured WBF-344 rat LEC. These results suggest airborne samples tested in this experiment may attribute to cause a certain type and degree of cancers in in vivo when exposured for some periods.

• Journal of The Korean Society of Agricultural Engineers
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• v.49 no.4
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• pp.63-70
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• 2007

The soil samples used in this study were prepared with the residual granite soils passed through the #4 sieve and obtained from Pocheon in Gyeonggi-Ki do. Specifically, five types of samples were prepared. Sample A which is a natural state soil was classified as SM according to the uses. Samples of B, C, D, and E were prepared by mixing Sample A with bentonite and were classified as SC according to the uses. The plasticity indexes of samples B, C, D, and E were 15, 25, 30, and 40, respectively. The specimens, whose dimensions are 100mm by 127.5mm, were prepared by A method of ASTM D 698 with the Proctor mold. In experiments, the loading with the 1%/min was applied. Four different disk diameters, 13mm, 26mm, 38mm, and 52mm were used in the Improved Unconfined Penetration (IUP) tests. To compare the tensile strengths, the split tensile tests were also conducted on the same specimens used for the IUP tests. To find out the effects of moisture contents, plasticity Index of specimens on the tensile strength, six different water contents were used for making a specimen.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.30 no.10 s.253
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• pp.1261-1268
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• 2006

This paper reports low-cost microreactor $(10{\mu}{\ell})$ biochip for the DNA PCR (polymerase chain reaction). The microbiochip $(20mm{\times}28mm)$ is a hybrid type which is composed of PDMS (polydimethylsiloxane) layer with serpentine micochannel $(360{\mu}m{\times}100{\mu}m)$ chamber and glass substrate integrated with microheater and thermal microsensor. Undesirable bubble is usually created during sample loading to PMDS-based microchip because of hydrophobic chip surface. Created bubbles interrupt stable biochemical reaction. We designed improved microreactor chamber using microfluidic simulation. The designed reactor has a coner-rounded serpentine channel architecture, which enables stable injection into hydrophobic surface using micropipette only. Reactor temperature needed to PCR reaction is controlled within ${\pm}0.5^{\circ}C$ by PID controller of LabVIEW software. It is experimentally confirmed that SRY gene PCR by the fabricated microreactor chip is performed for less than 54 min.

• Geomechanics and Engineering
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• v.11 no.5
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• pp.713-723
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• 2016

Shear wave velocities of three selected sandy soils subject to drained triaxial compression test were continuously measured using the bender elements. The shear wave velocity during isotropic compression, as widely recognized, increased as confining pressure increased and they were correlated well. However, during drained shearing, the mean effective stress could no further provide a suitable correlation. The shear wave velocity during this stage was almost constant with respect to the mean effective stress. The vertical stress was found to be more favorable at this stage (since confining stress was kept constant). When sample was attained its peak stress, the shear wave velocity reduced and deviated from the previously existed trend line. This was probably caused by the non-uniformity induced by the formation of shear band. Subsequently, void ratios computed based on external measurements could not provide reasonable fitting to the initial stage of post-peak shear wave velocity. At very large strain levels after shear band formation, the digital images revealed that sample may internally re-arrange itself to be in a more uniform loose stage. This final stage void ratio estimated based on the proposed correlation derived during pre-peak state was close to the value of the maximum void ratio.

• Journal of the Korean Geotechnical Society
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• v.19 no.2
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• pp.57-64
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• 2003

The liquefaction potential of saturated sands under seismic loading conditions has been carefully considered by many investigations. Typical of these investigations is the laboratory determination of cyclic strength of sands by means of cyclic triaxial tests. This study was conducted to investigate the effects of the method of sample preparation on the liquefaction characteristics of remolded samples of saturated uniform sands. Cyclic triaxial tests were performed on saturated uniform sand compacted to the same density by 3 different procedures of pluvial compaction through air, pluvial compaction through water and vibratory compaction. It was validated that the cyclic stress ratio of remolded saturated uniform sands by different compaction procedures at the same density was very different.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.8
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• pp.241-249
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• 2021

The aim of the research is to evaluate the impact of social media marketing (with special reference to Facebook) on the brand preference of customers with regard to smart mobile phones. Since Facebook has become very popular today and a trend has arisen to use social media as a marketing tool, the researcher intended to evaluate the impact of social media marketing on brand preference, as the findings would provide valuable insight for future businesses. Social media as measured social media visibility, social media engagement, and social media influencewas the independent variableand brand preference was the dependent variable. The convenience sampling method was used where the sample was taken from a group of people easy to contact or to reach. A sample of 186 young males and females was selected. Factor loading and factor analysis were used to analyze the data and find the most influencing factors on brand preference. Reliability analysis, validity analysis, and regression analysiswere performed to analyze the data. The R² value is 0.320 implying that 32.00% of the variance in brand preference is explained by social media influence, social media engagement, and social media visibility. The findings show thatsocial media visibility, social media engagement, and social media influencehave a positive impact on brand preference.

• Computers and Concrete
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• v.31 no.1
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• pp.71-84
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• 2023

In this paper, the effects of the thickness of cubic samples on the tensile strength of concrete blocks were studied using experimental tests in the laboratory and numerical simulation by the particle flow code in three dimensions (PFC3D). Firstly, the physical concrete blocks with dimensions of 150 mm×190 mm (width×height) were prepared. Then, three specimens for each of seven different samples with various thicknesses were built in the laboratory. Simultaneously with the experimental tests, their numerical simulations were performed with PFC3D models. The widths, heights, and thicknesses of the numerical models were the same as those of the experimental samples. These samples were tested with a new tensile testing apparatus. The loading rate was kept at 1 kg/sec during the testing operation. Based on these analyses, it is concluded that when the thickness was less than 5 cm, the tensile strength decreased by increasing the sample thickness. On the other hand, the tensile strength was nearly constant when the sample thickness was raised to more than 5 cm (which can be regarded as a threshold limit for the specimens' thickness). The numerical outputs were similar to the experimental results, demonstrating the validity of the present analyses.

• Journal of the Korean Wood Science and Technology
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• v.51 no.6
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• pp.526-541
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• 2023

Wooden structures are widely used, particularly in earthquake zones, owing to their light weight, ease of application, and resistance to the external environment. In this study, we aimed to improve the mechanical properties of laminated timber beams using novel hybrid systems [carbon-fiber-reinforced polymer (CFRP) and wire rope]. Within the scope of this study, it is expected that using wood, which is an environmentally friendly and sustainable building element, will be more economical and safe than the reinforced concrete and steel elements currently used to pass through wide openings. The structural behavior of the hybrid-reinforced laminated timber beams was determined under the loading system. The experimental findings showed that the highest increase in the values of laminated beams reinforced with steel ropes was obtained with the 2N reinforcement, with a maximum load of 38 kN and a displacement of 137 mm. Thus, a load increase of 168% and displacement increase of 275% compared with the reference sample were obtained. Compared with the reference sample, a load increase of 92% and a displacement increase of 14% were obtained. Carbon fabrics placed between the layers with fiber-reinforced polymer (FRP) prevented crack development and provided significant interlayer connections. Consequently, the fabrics placed between the laminated wooden beams with the innovative reinforcement system will not disrupt the aesthetics or reduce the effect of earthquake forces, and significant reductions can be achieved in these sections.