• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2733-2737
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• 2014

Objective: Molecular pathology tests are often carried for clinicopathological diagnosis and pathologists have established large collections of formalin-fixed, paraffin-embedded tissue (FFPE) banks. However, extraction of DNA from FFPE is a laborious and challenging for researchers in clinical laboratories. The aim of this study was to compare two widely used DNA extraction methods: using a QIAamp DNA FFPE kit from Qiagen and a Cobas Sample Preparation Kit from Roche, and evaluated the effect of the DNA quality on molecular diagnostics. Methods: DNA from FFPE non-small cell lung carcinoma tissues including biopsy and surgical specimens was extracted with both QIAamp DNA FFPE and Cobas Sample Preparation Kits and EGFR mutations of non-small cell lung carcinomas were detected by real-time quantitative PCR using the extracted DNA. Results and Conclusion: Our results showed that DNA extracted by QIAamp and Cobas methods were both suitable to detect downstream EGFR mutation in surgical specimens. Howover, Cobas method could yield more DNA from biopsy specimens, and gain much better EGFR mutation results.

• 생약학회지
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• 제37권3호
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• pp.143-150
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• 2006

Decoction of oriental medicinal preparation is prepared in various manners, and changes of chemical constituents might be occurred depending on the processing techniques. The present study was undertaken to investigate the phγsio-chemical and pharmacological equivalence between two extraction methods of Nokyong-sagunja-Tang. Samples were Prepared as follows ; Sample-I was prepared by simultaneously extracting Sagunja-Tang and velvet antler in one vessel. Sample-lI was prepared by adding velvet antler exact to the water extract of Sagunja-Tang. Both sanples showed similar results of physiochemical parameters such as pH, yield, TLC and HPLC chromatogram, and contents of ginsenoside $Rb_1$ and glycyrrhizin. Also, there were little different between two samples in pharmacological effects such as DPPH free radical scavenging effect, and inhibitory effects on xanthine oxidase, hyaluronidase, trypsin, TBA-Rs formation and hemolysis in vitro. And both samples showed no significant difference in antifatigue activities in mice. These results suggest that there might be little difference between two extraction process when velvet antler added to Sagunia-Tang.

• 한국콘텐츠학회논문지
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• 제16권4호
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• pp.757-769
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• 2016

본 연구는 중고령자의 노후준비가 삶의 만족도에 미치는 영향이 성별에 따라 차이가 있는지를 살펴보는 목적으로 분석하였다. 이를 위한 연구 자료는 국민노후보장패널 3차년도 부가조사, 4차년도 자료를 이용한 연구로서 3차년도 기준 50세 이상 64세 미만으로 남녀 중고령자 2,970명을 대상으로 분석하였다. 분석 결과 첫째, 성별에 따른 노후준비의 차이에서는 남녀 모두 통계적으로 유의미하게 차이가 있는 것으로 나타났다. 둘째, 성별에 따른 삶의 만족도 차이에서 남녀 모두 통계적으로 유의미한 차이가 있는 것으로 나타났다. 셋째, 노후준비가 삶의 만족도에 미치는 영향에 대하여 성별 차이를 비교한 결과 남녀 모두 유의미하게 차이가 있는 것으로 확인되었다. 위의 연구결과를 바탕으로 하여 중고령자의 노후준비에 따른 삶의 만족도 영향에 대한 성별 비교를 통하여 성별에 따른 노후준비와 삶의 만족도를 높이기 위한 방안과 후속연구를 위한 제언을 하였다.

• Journal of Korean Biological Nursing Science
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• 제22권1호
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• pp.53-60
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• 2020

Purpose: This study used raw data from the fifth (2010-2012) Korea National Health and Nutrition Examination Survey (KNHANES) to assess the relationship between vitamin D level and glycemic control of diabetes, and to provide basic data about the use of vitamin D for preparation of a treatment plan for diabetes in South Korea. Methods: For this study, data of 1,713 diabetes from KNHANES (2010-2012) were used. The collected data were analyzed using SPSS 18.0 program, and complex sample frequency analysis, descriptive statistics, complex sample cross analysis, complex sample general linear regression, and complex sample logistic regression analysis were performed. Results: It was found that the poor glycemic control group among the diabetes subjects had significantly lower level of blood vitamin D than the good glycemic control group. Factors affecting glycemic control included drinking, vitamin D levels, hypertriglyceridemia, duration of diabetes, and treatment of diabetes. Also, diabetics with vitamin D deficiency or shortage showed 3.55- and 2.61-times higher odds ratios, respectively, to be diagnosed as the poor glycemic control group than diabetics without vitamin D deficiency or shortage. Conclusion: This study is significant because it provides rationale and basic data about the use of vitamin D for preparation of a treatment plan for diabetes in South Korea by assessing the dependence of glycemic control on the vitamin D level of diabetics. Additionally, future studies are necessary to determine the appropriate concentration of vitamin D for diabetes prevention and treatment to prevent the side effects of excessive supplementation.

• Journal of Pharmaceutical Investigation
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• 제40권3호
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• pp.193-200
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• 2010

The objective of this study was to investigate the effects of sample preparation, HPLC conditions and peak measurement methods upon determining progesterone content of poly-d,l-lactide-co-glycolide microspheres. A series of the microspheres with different formulations was first prepared. To determine their actual drug contents, the microspheres were dissolved in tetrahydrofuran and diluted with various amounts of methanol to precipitate the polymer. After removal of polymeric precipitates, the filtrates were subject to HPLC analysis under versatile experimental conditions. Interestingly, the composition of a sample solution (e.g., the ratio of methanol to tetrahydrofuran) affected the magnitudes of both peak fronting and peak broadening of progesterone. Its peak became broader and more asymmetrical at lower methanol:tetrahydrofuran ratios. Furthermore, its peak height was influenced by the proportion of tetrahydrofuran in a sample solution. Such problems encountered with tetrahydrofuran were exacerbated when a larger volume of the sample solution was injected onto an analytical column. Under our experimental conditions a peak area measurement provided more accurate and reliable determination of progesterone content in various microspheres than a peak height determination. Optimizing the composition of a sample solution, HPLC chromatographic conditions and peak analysis methods was a prerequisite to an accurate determination of progesterone encapsulated within microspheres.

• Near Infrared Analysis
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• 제1권2호
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• pp.35-39
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• 2000

The purpose of this research was to find out suitable soil sample preparation and sample holding tools for NIR reflection radiation for estimating soil components. NIR reflectance was scanned at 2nm intervals from 1,100 to 2,500nm with an InfraAlyzer 500(Bran+Luebbe Co.). Coarse(2.0mm) and fine(0.5mm) soil sample and various sample holding tools were used to obtain mean diffuse reflection of the soil for the calibration and validation of the calibration set in estimating moisture, organic matter and total nitrogen of the soils. Multiple linear regression was used to obtain the best correlation of NIR spectroscopy method. Correlation of NIR spectroscopy method. Correlation of NIR spectra for finely and coarsely sized soil did not show much difference. The standard errors of prediction(SE) using different types of sample holding tools for organic matter, total nitrogen and soil moisture were better than 0.765, 0.041 and 0.63% respectively. From the results it can be concluded that NIR spectroscopy with flow type cell could be used as a fast routine testing method in quantitative determination of organic matter, total nitrogen and soil moisture.

• Geomechanics and Engineering
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• 제27권6호
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• pp.615-626
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• 2021

The main objective of this study is to present a fast and reliable approach to predict the swelling potential of clay-bearing rocks. Investigations showed that there is a good correlation between the swelling potential of a rock and its desire to absorb water due to its clay content which could be measured using the "Contact Angle" test as one of the most common ways to determine the wettability. In this test, the angle between a water drop and the flat rock surface on which it rests is measured. The present method is very fast and returns repeatable results and requires minimal sample preparation. Only having a saw-cut surface of a sample with any shape is all one needs to perform this test. The logic behind this approach is that the swelling potential of a rock is a function of its mineral content and molecular structure, which are not only distributed in the bulk of the sample but also reflected on its surface. Therefore, to evaluate swelling behavior, it is not necessary to wait for a sample to get wet all the way to its "internal structure" (which, due to the low permeability of clay-bearing rocks, is very slow and time-consuming). Instead, one can have a good sense of swelling potential by studying its surface. Parametric studies on the effect of moisture content, porosity, and surface roughness on the contact angle measurements showed that using a saw-cut oven-dried sample is a convenient way to evaluate the swelling potential by this method.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1994년도 추계학술대회 논문집
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• pp.65-66
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• 1994

This paper describes study on the preparation of ferrite powder for bonded mallets. The specimen which has the basic composition of SrO$.$nF$_2$O$_3$ with n=5.9 is in nonstoichiomatric region. Calcination is performed under N$_2$ atmosphere 1175$^{\circ}C$, 1200$^{\circ}C$, 1225$^{\circ}C$, 1250$^{\circ}C$ and 1275$^{\circ}C$ respectively. Then, Cooling is carried out in the furnace. In order to increase coecivity and obtain uniform grain size, we add to the specimen 0.7wt%CaCO$_3$, 0.3wt % SiO$_2$, 0.5wt%Na$_2$SiO$_3$ and 0.5wr% Al$_2$O$_3$. Also, in order to increase milling effect, carbon coating on sample particles is tried. As the result, single magnetic domain partic1e with Size of 1$\mu\textrm{m}$ in obtained and magnetic properties are improved.

• 한국융합학회논문지
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• 제9권1호
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• pp.473-483
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• 2018

본 연구목적은 대장내시경 전처치 융합관리프로그램의 효과를 확인하기 위함이다. 비동등성 대조군 시차설계에 따라 대장내시경 검진예정자 75명을 연구대상으로 하였다. 실험군에게는 동영상교육, 걷기운동 및 전화상담모니터링으로 구성한 대장내시경 전처치 융합관리프로그램을 적용하였다. 자료는 구조화된 질문지와 대장내시경 관찰을 통해 수집하였으며 SPSS 21.0 program을 이용하여 분석하였다. 연구결과 실험군의 대장정결제 복용이행도와 검사만족도는 대조군보다 높았고(p=.002; p=.001), 검사난이도와 검사불편감은 유의하게 낮았다(p=.002; p=.001). 대장정결도와 검진소요시간에는 두 집단 간 유의한 차이가 있었으나 식이요법이행도는 차이가 없었다((p<.001; p=.001; p=.108). 따라서 대장내시경 전처치 융합관리프로그램은 대장내시경검사를 위한 효과적인 간호중재방안으로 활용될 수 있으며 향후 임상실무에서 다양한 진단적 검사를 위한 융합중재개발이 요구된다.

• 한방안이비인후피부과학회지
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• 제19권1호
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• pp.43-54
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• 2006

Objective : In order to investigate anti-inflammatory effect of Alum objectively which is traditional remedy applicated by external preparation frequently, especially in Rhinologic field. Materials and Method : We studied the histopathologic and hematologic features, serum transaminase activities in the experimentally induced maxillary sinusitis in rabbits. 9 rabbits was divided into normal, control anti sample group(each 3 rabbits). We inoculated $10^{10}$ P. aeruginosa for experimental maxillary sinusitis to control group and sample group. Sample group was treated with aim solution(l0g/100cc) lcc via both nasal cavity (each 0.5cc) after 24hrs everyday for 14days. Results and Conclusion : 1. We confirmed that maxillary sinusitis was well induced by P. aeruginosa without occlusion of maxillary ostium. 2. There was no abnormal findings in serum transaminase(AST/ALT) activities even though application of Alum solution on nasal mucosa for 14days continuously. 3. Alum has evident anti-inflammatory effect of recovering mucosal surface injury, reduction of goblet cell, lymphocyte infiltration, edema and expansion of glandular tissue, dilatation and congestion of blood vessels and so on. 4. Alum has the effect that recover glandular tissue injury and decrease goblet cell increase by the result of dermal PAS staining increase and epidermal AB staining decrease in the qualitative analysis of epidermal and dermal mucopolysaccharide