• Title/Summary/Keyword: Sample preparation effect.

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Quality Characteristics of Apricot Sulgidduk by the Saccharides Assortment (당 종류별 살구설기떡의 품질특성)

  • Shin, Young-Ja;Park, Geum-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.2
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    • pp.233-240
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    • 2007
  • The purpose of this study was to evaluate the effect of different saccharides on the quality of apricot sulgidduk. Appearance color of honey-added group as compared to other samples in apricot sulgidduk were preferable and scanning electron microscopy exhibited smaller pores. pH and acidity of white-added group immediately after preparation were higher than those of other groups. Higher moisture contents of honey-added groups during storage were found but total bacterial counts immediately before storage were 1 log cycle lower than that of other samples. The highest 'L' values were found for white sugar added group but the highest 'a' and 'b' were for yellow sugar-added sample. In mechanical characteristics of apricot sulgidduk for 48 hrs during storage at $20^{\circ}C$, hardness, gumminess and brittleness were higher in oligosaccharide-added sample as compared to other samples, while cohesiveness and springiness were the highest in honey-added group. In sensory evaluation of the sulgidduk, honey-added group obtained the highest score in color, sweet taste, moisture, after swallowing and overall quality (p<0.05) These results suggest that apricot sulgidduk with honey can be used as commercially successful rice cakes.

Method for Screening and Confirming Meldonium in Human Urine by High-Resolution Mass Spectrometry and Identification of Endogenous Interferences for Anti-Doping Testing

  • Kim, Yongseok;Jeong, Dawon;Min, Hophil;Sung, Changmin;Park, Ju-hyung;Son, Junghyun;Lee, Kang Mi;Kim, Ho Jun;Lee, Jaeick;Kwon, Oh-Seung;Kim, Ki Hun
    • Mass Spectrometry Letters
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    • v.8 no.2
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    • pp.39-43
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    • 2017
  • Meldonium is a drug for treating ischemia by expanding the arteries but it can also enhance the performance of sports players. The World Anti-Doping Agency (WADA) has included it in the list of prohibited substances since 2016. Meldonium is one of the challenging substances for anti-doping testing because it is difficult to recover by general liquid-liquid or solid phase extraction due to its permanent charge and high polarity. Therefore, high-performance liquid chromatography (HPLC) is currently used by injecting a diluted urine sample (known as the "dilute-and-shoot" strategy). There is no loss of target compounds in the extraction/cleanup procedure but its high matrix effect could interfere in their separation or detection from the endogenous urinary compounds. We report a single method using high-resolution mass spectrometry that can be used for both screening and confirmation, which follows the "dilute-and-shoot" strategy. In this method, the endogenous compounds' interfering peaks in the mass spectrum are separated at a high resolution of FWHM 140,000, and the results are suitable for substance detection following the WADA guidelines. The interferences in the obtained mass spectrum of the urine matrix are identified as acetylcholine, lysine, and glutamine by further analysis and database searching. Validation of the method is performed in routine anti-doping testing, and the limit of detection is 50 ng/mL. This method uses simple sample preparation and a general reverse phase HPLC column, and it can be easily applied to other substances.

Properties of Quercus variabilis bio-oil prepared by sample preparation (시료 조건에 따른 굴참나무 바이오오일의 특성)

  • Chea, Kwang-Seok;Jo, Tae-Su;Choi, Seok-Hwan;Lee, Soo-Min;Hwang, Hye-Won;Choi, Joon-Weon
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.1
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    • pp.148-156
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    • 2015
  • In this study the differences in the sample size and sample input changes as characteristics of bio-oil oak(Quercus variabilis), the oak 0.5~2.0 mm of the oak weighing 300~900g was processed into bio-oil via fast pyrolysis for 1.64 seconds. In this study, the physico-chemical properties of biooil using oak were investigated. Fast pyrolysis was adopted to increase the bio-oil yield from raw material. Although the differences in sample size and sample input changes in the yield of pyrolysis products were not significantly noticeable, increases in the yield of bio-oil accounted for approximately 60.3 to 62.1%, in the order of non-condensed gas, and biochar. When the primary bio-oil obtained by the condensation of the cooling tube and the seconary bio-oil obtained from the electric dust collector were measured separately, the yield of primary bio-oil was twice as higher than that of the secondary bio-oil. However, HHV (Higher Heating Value) of the secondary bio-oil was approximately twice as higher than that of the primary bio-oil by up to 5,602 kcal/kg. The water content of the primary bio-oil was more than 20% of the moisture content of the secondary bio-oil, which was 10% or less. In addition, the result of the elemental analysis regarding the secondary bio-oil, its primary carbon content was higher than that of the primary bio-oil, and since the oxygen content is low, the water content as well as elemental composition are believed to have an effect on the calorific value. The higher the storage temperature or the longer the storage period, the degree of the viscosity of the secondary bio-oil was higher than that of the primary bio-oil. This can be the attributed to the chemical bond between the polymeric bio-oil that forms during the storage period.

Compensation of Surface Temperature Effect in Determination of Sugar Content of Shingo Pears using NIR (근적외선을 이용한 신고 배 당도판정에 있어 표면 온도영향의 보정)

  • 이강진;최규홍;김기영;최동수
    • Journal of Biosystems Engineering
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    • v.27 no.2
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    • pp.117-124
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    • 2002
  • This research was conducted to develop a method to remove the effect of surface temperature of Shingo pears for sugar content measurement. Sugar content was measured by a near-infrared spectrum analysis technique. Reflected spectrum and sugar content of a pear were used for developing regression models. For the model development, reflected spectrums having wavelengths in the range of 654 to 1,052nm were used. To remove the effect of surface temperature, special sample preparation techniques and partial least square (PLS) regression models were proposed and tested. 71 Shingo pears stored in a cold storage, which had 2$^{\circ}C$ inside temperature, were taken out and left in a room temperature for a while. Temperature and reflected spectrum of each pear was measured. To increase the temperature distribution of samples, temperature and reflected spectrum of each pear was measured four times with one hour twenty minutes interval. During the experiment, temperature of pears increased up to 17 $^{\circ}C$. The total number of measured spectrum was 284. Three groups of spectrum data were formed according to temperature distribution. First group had surface temperature of 14$^{\circ}C$ and total number of 51. Second group consisted of the first and the fourth experiment data which contained the minimum and the maximum temperatures. Third group consisted of 155 data with normal temperature-distribution. The rest data set were used for model evaluation. Results shelved that PLS model I, which was developed by using the first data group, was inadequate for measuring sugar content of pears which had different surface temperatures from 14$^{\circ}C$. After temperature compensation, sugar content predictions became close to the measured values. Since using many data which had wide range of surface temperatures, PLS model II and III were able to predict sugar content of pears without additional temperature compensation. PLS model IV, which included the surface temperatures as an independent variable. showed slightly improved performance(R$^2$=0.73). Performance of the model could be enhanced by using samples with more wide range of temperatures and sugar contents.

The Impact of Meaning in Work on Happiness in Mid-aged: Mediating effect of Meaning of Life (중년 성인의 일의 의미가 행복에 미치는 영향: 삶의 의미의 매개 효과)

  • Jo, Seolae;Jeong, Youngsook;Lee, Jaesik;Joo, Mijung
    • Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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    • v.8 no.10
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    • pp.871-882
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    • 2018
  • This study investigated meaning of life as mediator of the relationships of meaning of work and happiness in Koran middle aged. A sample of 171 middle aged were participated. in survey about meaning of work, meaning of work and happiness. The data were analyzed using correlation test and path analysis. The results was followed. First, every factors was positively correlated. The meaning of work was positively correlated with meaning in life and happiness. And meaning in life was also positively correlated with happiness. Second, the meaning in life had partial mediation effect between meaning of work and happiness. The middle aged's higher score of meaning of work not only directly increased happiness, but also induced higher level of meaning in life, which, in turn, increased happiness. The results of this study suggested that meaning of work is a predictor meaning in life and happness, and meaning in life can be important factor of the relationship between meaning of work and happiness.

A Study on the effect of Friend-praise activity program of Elementary students for Self-esteem and Personal Relationship (초등학생의 또래 칭찬활동 프로그램이 자아존중감 및 대인관계에 미치는 효과)

  • Oh, Eun-Ju
    • The Korean Journal of Elementary Counseling
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    • v.5 no.1
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    • pp.159-184
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    • 2006
  • Lately, the rapid social and family system change, the entrance examination-centered circumstance in education have a negative influence not only on making up the affirmative self-esteem but also on building up the personal relationship through mutual communication with friends of the same age. Making children adapt well to school life and develop a good relationship in the class is an important subject in educational field. Thus, various school activities should offer lots of opportunities to increase their affirmative self-esteem and more specified performance-centered programs are required toform a harmonious relationship among parties of students. The purpose of this study is to improve an affirmative self-esteern and a desirable personal relationship of children through developing friend-praise program and adapting it to children. Based on the above purpose, the following subjects for this study 'are suggested. Firstly, is the friend-praise program effective in forming an affirmative self-esteem of elementary students? Secondly, is the friend-praise program successful in improving a desirable personal relationship in an elementary school? In addition, two hypotheses of study are decided on the basis of theoretical background and the former study. Hypothesis 1. The friend-praise program will have a meaningful effect on improving an affirmative self-esteem. Hypothesis 2. The friend-praise program will have a meaningful effect on improving a desirable personal relationship. In order to improve the above hypotheses, the experiment was carried out with two classes on the fourth grade in M elementary school in Wonju with being divided into one experimental group and the other comparative group. The friend-praise program which was adapted to the experimental group was composed with four steps as 'introduction', 'preparation', 'operation' and 'closing'. This program which has twelve categories was executed once or twice in a week and each class was continued for forty or sixty minutes. Contrastively, comparative group had no application of the main program. For the purpose of proving the hypotheses after adapting the program to students, I have executed both self-esteem test and personal relationship test. The tests were performed with using SPSS/Windows V10.0 and the statistic was conducted through independant sample t-test for the difference between groups and contrastive sample t-test for the individual difference in each group. The results of this study can be summarized like the followings: Firstly, from the result of previous and after tests, there was no meaningful change in the comparative group, but the experimental group showed meaningful difference in all categories of self-esteem(t=-4.496, p=.000). Especially, the general self-esteem(t=-3.216, p=.003), social self-esteem(t=-2.680, p=.012), homely self-esteem(t=-3.732, p=.001), and school self-esteem(t=-3.902, p=.000) showed a meaningful difference. At the same time, the experimental group also showed meaningful difference with the comparative group in self-esteem(t=-4.758, p=.000). Especially, the general self-esteem(t=2.581, p=.017), social self-esteem(t=3.160, p=.003), homely self-esteem(t=4.283, p=.000), and school self-esteem(t=4.110, p=.000) showed a meaningful difference. As a result of this experiment, the friend-praise program will have a meaningful effect on improving an affirmative self-esteem was proved. Secondly, there was no meaningful change in the comparative group, but the experimental group showed meaningful difference in all categories of personal relationship(t=-4.131, p=.000). Specifically, satisfaction(t=-2.113, p=.045), communication(t=-3.381, p=.002), confidence(t=-3.517, p=.001), intimacy(t=-3.958, p=.000), sensibility(t=-2.955, p=.006), openness(t=-4.318, p=.000) and interest(t=-2.941, p=.000) showed a meaningful difference. In the same instant, the experimental group also showed meaningful difference with the comparative group in all categories of personal relationship(t=3.897, p=.000). Especially, satisfaction(t=2.257, p=.003), communication(t=3.527, p=.001), confidence(t=3.704, p=.001), intimacy(t=3.904, p=.000), sensibility (t=4.382, p=.000), openness(t=2.648, p=.013) and interest(t=2.944, p=.006) showed a meaningful difference. Accordingly, the friend-praise program will have a meaningful effect on improving a desirable personal relationship was proved. Judging from all these results, we should provide primary students with various opportunities to take part in active programs which help them to set up their righteous sense of value, to solve their own problems and to develop their potentials. Consequently, many kinds of practice-centered program like the friend-praise program should be developed more systematically and teachers should apply all those programs to students according to their individual level and developmental stage.

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Hydrophobic Coating on Fish Feed Using Dielectric Barrier Discharge Plasma Polymerization (유전체장벽방전 플라즈마 중합을 이용한 양어 사료의 소수성 코팅)

  • Lee, Sang Baek;Hung, Trinhquang;Jo, Jin Oh;Jung, Jun Bum;Im, Tae Heon;Mok, Young Sun
    • Applied Chemistry for Engineering
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    • v.25 no.2
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    • pp.174-180
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    • 2014
  • A plasma hydrophobic coating on commercial fish feed was conducted to prolong the floating time of feed, thereby enhancing the feed consumption rate and reducing the contamination of water in fish farms. The hydrophobic coating on the fish feed was prepared using an atmospheric-pressure dielectric barrier discharge (DBD) plasma with hexamethyldisiloxane (HMDSO), toluene and n-hexane as the precursors. The effect of the parameters such as input power, precursor type and coating time on the coating performance were examined. The physicochemical properties of the coating layer were analyzed using a Fourier transform infrared (FTIR) spectrometer and a contact angle (CA) analyzer. The water CA increased after the coating preparation, indicating that the surface changed from hydrophilic to hydrophobic. The FTIR characterization revealed that the hydrophobic layer was comprised of functional groups such as $CH_3$, Si-O-Si and Si-C. As a result of the hydrophobic coating, the floating time of the fish feed increased from several seconds to 3 minutes, which suggested that the plasma coating method could be a viable means for practical applications. Compared to the water CA measured as soon as the coating layer was prepared, the 6-day aged sample exhibited a substantial CA increase, confirming the aging effect on the improvement of the hydrophobicity.

Effect of Selected Environmental Factors on the Production of Geosmin in Phormidium Sp. (Phormidium sp.의 Geosmin 생산에 미치는 환경요인의 영향)

  • 박대균;오희목;안치용;맹주선
    • Korean Journal of Microbiology
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    • v.36 no.1
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    • pp.52-57
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    • 2000
  • A method for quantitative and qualitative analysis of geosmin, odorant produced by several actinomycetes and cyanobacteria, was established and optimized. The effects of environmental conditions on the growth of Phormidium sp. NIVA-CYA7 were examined and the production and release of geosmin by the species was analyzed by using the purge and trap-gas chromatographic technique. One of the major advantages of the technique established in the present study is that the preparation of sample is simpler and purge time is shorter. Under the culture conditions (pH 7.9, $20^{\circ}C$, 120-140 $\mu$E/$m^2$/s and Z8 medium), Phormidium showed growth characteristics with a lag phase for 8 days and an exponential phase for 14 days followed by a stationary phase. Reduction of inorganic nitrogen concentrations in the culture medium from 250 to 100 or 25 $\mu$M brought no significant effect on the cell growth. However, the cell growth was significantly inhibited with decreasing concentrations of inorganic phosphorus from 25 to 10 or 2.5 $\mu$M. When the inorganic phosphorus concentration in the medium was lowered from 25 to 10 $\mu$M, the levels of geosmin in the organism expressed as percentages per unit TOC and chlorophyII-$\alpha$ increased by 35% and 68%, respectively. When the initial pH of the medium was 9.4, geosmin content was 0.0824 $\mu$g/mg C, which was 2-fold higher than that at pH 7.9 Consequently, the level of geosmin in Phormidium was found to vary with growth phases of the culture, external inorganic phosphorus concentration and external pH, while the release of geosmin was not significantly affected by the factors.

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Effect on NCOs and students of self-leadershiployment career (부사관과 학생들의 셀프리더십이 취업진로에 미치는 영향)

  • Kwon, Jung-Min;Lee, Han-Kyu
    • Convergence Security Journal
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    • v.17 no.2
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    • pp.109-118
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    • 2017
  • The study examines whether there is support for undergraduate students of Department of NCOs leadership needs and self-perceived any casualty, the purpose being placed to identify the cause-and effect relationship between student's behavior and these self-appointed leadership needs parameters. To study this end, the men and women college students Military major in Busan district using the convenience of the student sample extraction to extract the 362 students. Setting the model to achieve the object of the study, and then through a structural equation model (SEM) were studies a causal relationship among variables. Result on the basis of the research study model verification method as described above what is derived from this study were as follows. First, self-leadership is confirmed in the career planning of clarity on the impact of career beliefs centered strategies(+) target-oriented strategy(+), and independent self-reliance, check-centered strategies(+), constructive thinking strategies(+), ERA=centric strategy(+), in the natural course flexibility, compensation strategies(+), constructive thinking strategies(+) improve professional skills appeared to affect the check-centered strategies(+), ERA-centered strategies(+). Second, self-leadership is general satisfaction at the impact of major satisfaction natural reward strategies(+), the curriculum meets the natural reward strategies(+) target-oriented strategy(+) recognition satisfy the natural reward strategies(+) target-oriented strategy(+) appeared to affect this. Third, career beliefs Major General satisfaction in the impact on satisfaction Career Planning Clarity(+), an independent self-reliance(+), career flexibility(+)improve professional skills(+), the curriculum satisfies independent self-reliance(+), career flexibility(+) improve professional skills(+), the self-satisfied recognized independent trust(+), career flexibility(+), career planning clarity(+) it appeared to influence this.

Inhibitory Effect on the Growth of Intestinal Pathogenic Bacteria by Kimchi Fermentation (김치 발효에 의한 장내병원균의 생육저해효과)

  • Kang, Chang-Hoon;Chung, Kyung-Oan;Ha, Duk-Mo
    • Korean Journal of Food Science and Technology
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    • v.34 no.3
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    • pp.480-486
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    • 2002
  • Six strains of intestinal pathogenic bacteria were inoculated into kimchi at the preparation time, and the influence of kimchi fermentation on the growth of these pathogenic bacteria was investigated. The population of coliform bacteria in the kimchi raw materials, and its changes in the kimchi sample during fermentation were also determined. Among the raw materials, highest populations of coliform bacteria were detected in ginger and green onion, followed by Chinese cabbage, red pepper, and garlic. Populations of pathogenic bacteria (inoculated strains) and coliform bacteria in kimchi decreased as pH decreased with fermentation. Coliform bacteria disappeared at pH 3.9 in Chinese cabbage kimchi samples. Clostridium perfringens ATCC 13124, Listeria monocytogenes ATCC 19111, Salmonella typhimurium KCTC 1625, Staphylococcus aureus KCTC 1621, Vibrio parahamolyticus ATCC 27519, and Escherichia coli O157 H:7 ATCC 43894 were not detected at pH values less than 4.1, 3.7, 3.8, 3.8, 3.7, and 3.7 in Chinese cabbage kimchi, and at pH values less than 4.5, 4.0, 4.2, 4.2, 4.2 and 4.1 in mustard leaf kimchi, respectively. The juice of mustard leaf and allyl isothiocyanate exhibited high antimicrobial activities on the pathogenic bacteria, whereas the lowest on lactic acid bacteria. These results indicated that fermentation is useful to improve the safety of kimchi, and the antimicrobial effect of mustard leaf kimchi is mainly due to the major pungent compound of mustard leaf, allyl isothiocyanate.