• Title/Summary/Keyword: Sample Size

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Primary Culture of Human Hepatocytes from Small Size Sample

  • Oh, Goo-Taeg;Ahn, Chang-Joon;Ahn, Byung-Min;Hyun, Byung-Hwa;Choi, Jae-Yoon;Kim, Hwan-Mook
    • Toxicological Research
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    • v.8 no.2
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    • pp.285-302
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    • 1992
  • Human and rat hepatocytes were isolated by nonperfusion method and cultured for longer than 5 days. Human liver biopsy sample and rat liver were used as hepatocyte source. Several physical and chemical factors which were influencing on hepatocyte isolation procedure were examined and a batch isolation procedure was established for small size sample of rat liver. Isolated hepatocytes showed normal morphlologica characteristics in microscopy and electron microscopical examinations and a morphologica response to phalloidin. Isolated cells were cultured as a monolayer and proven to have intact morphological characteristics for longer than 15 days. Because human liver sample is harder and tighter compared with rat liver, a standard procedure for rat hepatocytes was slightly modified to reduce mechanical damage. Similarly with rat hepatocytes, isolated human hepatocytes showed a normal morphological characteristics and could be cultured for longer than 15days. Human and rat hepatocytes were examined on their functional integrities including cytochrome-P450 related enzyme activity and it's inducibility, hormonal inducibility of AIB uptake and TAT activity, albumin synthesis, DNA synthesis, cellular protein maintenance. In all parameters used in the present study, human and rat hepatocytes showed normal functional characteristics.

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Synthesis and Physico-Chemical Properties of Dicalcium Phosphate Dihydrate for Dental Abrasive (치아 연마용 인산일수소칼슘의 합성 및 물리화학적 성질)

  • 서성수;황성주;이기명;이계주
    • YAKHAK HOEJI
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    • v.37 no.1
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    • pp.66-75
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    • 1993
  • Dental abrasive, dicalcium phosphate dehydrate (DCPD) was prepared and the several important factors affecting on the quality of toothpaste were investigated by means of set test, glycerine absorption, Coulter counter test, color difference, BET adsorption, mercury porosimetery, and rheogram comparing with two foreign DCPDs, MFO4 and Dentphos K. Sample DCPD was prepared by reaction between 85% H$_{3}$PO$_{4}$ and 15% milk of lime at $39^{\circ}C$ (pH6.5), and stabilized with TSPP and TMP. The physicochemical properties of Sample DCPD were obtained as follows: whiteness (98.99), average particle size (15.5 $\mu\textrm{m}$), pH (7.9), remainder particle weight (0.49w/w%), glycerine absorption value (64 ml), and set test (passed). N$_{2}$ adsorption curves (BET) of three kinds of DCPD showed non-porous type III isotherm. BET adsorption parameters of sample DCPD showed that surface area was 24.9 m$^{2}$/g, total pore volume 0.09 cm$^{3}$/g and average pore radius 72.0 $\AA$. The rheogram of the toothpaste containing each DCPD showed bulged plastic flow with yield vlaue and thixotropic behavior. These results meet standard requirements as abrasive standard, and suggested that synthesized sample DCPD could be used a dental abrasive such as a high quality grade in practice as foreign DCPDs.

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Tree image comparison analysis using LBP method (LBP 방식을 이용한 나무 영상 비교 분석)

  • Kim, Ji-hong;Lee, Jonghyun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.25 no.4
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    • pp.530-536
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    • 2021
  • Since the LBP algorithm has the characteristic of local texture expression, it is possible to obtain completely different results depending on the extraction location and the size of the reference image and the sample image. In order to solve these shortcomings, in this paper, we first investigate the basic characteristics of LBP, make the size of the reference image (100×100) in order to include most of the characteristics in the image, and select a sample image (40×40) extracted from an arbitrary point. After finding the matching position in the LBP of the reference image by using the correlation test between the LBP of the reference image and the LBP of the sample image, a chi analysis method is used to find the reference image that most closely matches the sample image.

Influence of Dicyclopentadiene Resin on Abrasion Behavior of Silica-Filled SBR Compounds Using Different Abrasion Testers

  • Eunji Chae;Seong Ryong Yang;Seok Hyun Cho;Sung-Seen Choi
    • Elastomers and Composites
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    • v.58 no.3
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    • pp.103-111
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    • 2023
  • The abrasion resistances of silica-filled styrene-butadiene rubber (SBR) compounds prepared with and without dicyclopentadiene resin (SBR-R and SBR-0, respectively) were studied using four different abrasion testers, namely cut and chip (CC), Lambourn, DIN, and laboratory abrasion tester (LAT100). The effect of the resin on the abrasion behavior was elucidated by analyzing the morphologies and size distributions of wear particles. All the wear particles had rough surfaces, but those obtained in the Lambourn abrasion test exhibited relatively smooth surfaces. The size distributions of the wear particles showed different trends depending on the abrasion tester and the rubber compound; however, most of the wear particles were larger than 1000 ㎛. The SBR-R sample showed a wide range of particle sizes (from 63 ㎛) in the LAT100 abrasion test and majority of the wear particles were 500-1000 ㎛, whereas the SBR-0 sample had the most distribution of larger than 1000 ㎛. The abrasion rates of SBR-0 sample were lower than those of the SBR-R sample for the CC and LAT100 abrasion tests, but the Lambourn abrasion test result showed the opposite trend. Addition of the resin influenced the abrasion behavior, however the effect varied depending on the type of abrasion tests.

A Comparison of Analysis Methods for Work Environment Measurement Databases Including Left-censored Data (불검출 자료를 포함한 작업환경측정 자료의 분석 방법 비교)

  • Park, Ju-Hyun;Choi, Sangjun;Koh, Dong-Hee;Park, Donguk;Sung, Yeji
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.32 no.1
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    • pp.21-30
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    • 2022
  • Objectives: The purpose of this study is to suggest an optimal method by comparing the analysis methods of work environment measurement datasets including left-censored data where one or more measurements are below the limit of detection (LOD). Methods: A computer program was used to generate left-censored datasets for various combinations of censoring rate (1% to 90%) and sample size (30 to 300). For the analysis of the censored data, the simple substitution method (LOD/2), β-substitution method, maximum likelihood estimation (MLE) method, Bayesian method, and regression on order statistics (ROS)were all compared. Each method was used to estimate four parameters of the log-normal distribution: (1) geometric mean (GM), (2) geometric standard deviation (GSD), (3) 95th percentile (X95), and (4) arithmetic mean (AM) for the censored dataset. The performance of each method was evaluated using relative bias and relative root mean squared error (rMSE). Results: In the case of the largest sample size (n=300), when the censoring rate was less than 40%, the relative bias and rMSE were small for all five methods. When the censoring rate was large (70%, 90%), the simple substitution method was inappropriate because the relative bias was the largest, regardless of the sample size. When the sample size was small and the censoring rate was large, the Bayesian method, the β-substitution method, and the MLE method showed the smallest relative bias. Conclusions: The accuracy and precision of all methods tended to increase as the sample size was larger and the censoring rate was smaller. The simple substitution method was inappropriate when the censoring rate was high, and the β-substitution method, MLE method, and Bayesian method can be widely applied.

A study on sampling design for house price survey in city area (전국 도시 주택가격 동향조사를 위한 표본설계 연구)

  • 이기재;박진우;박홍래
    • The Korean Journal of Applied Statistics
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    • v.4 no.2
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    • pp.137-148
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    • 1991
  • This paper describes the design of sample of the survey on the trend of house prices in city areas. The purpose of this research is to increase the precision of house price index in 39 cities and to provide with an accurate house price indes. The sample is selected in the stratified two stage sampling. In chapter 2, review and discussions are given on the sample design now in use. In chapter 3, we describe the sample size and the stratification, the house price index and error, and the substitution of sample. Finally we consider on problems of the sample design and some alternatives to solve them.

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Selection of Grading Deviations to Develop Improved Baseball Leg Guards for Size Suitability and Efficiency (사이즈 적합성과 효율성이 향상된 야구 다리보호대 개발을 위한 그레이딩 편차 선정)

  • Lee, Hyojeong;Eom, Ran-i;Lee, Yejin
    • Journal of the Korean Society of Clothing and Textiles
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    • v.41 no.1
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    • pp.184-195
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    • 2017
  • Baseball is a life sport that gives vitality to people. However, currently available leg guards do not have a variety of sizes and it is very difficult to purchase a leg guard that is suitable for the size of a specific consumer. Therefore, this study investigated the size system of a leg guard sold in the market and suggested a grading deviation suitable for the legs of Korean males. The results of the study were as follows. First, most brands were sold only in three size systems as children's, teenagers, and adults. Adult size systems were not subdivided. Second, Korean male legs were classified into Group 1 (large girth and height and lower body muscle) and Group 2 (small girth and height and low underbody muscle). Third, the size system is based on vertical items such as height and mid-thigh circumference. Each group produced 10 sizes. The smallest height or the largest height was also found to be about 4.5 to 5.0cm smaller or larger than the sample size for each group. The total length of the leg guard was about 2.5cm when the height was increased or decreased by one size. It was confirmed that the deviation of the circumference of the mid-thigh should be about 2.0cm larger or smaller than the sample size even if the height is the same. The deviation of the knee circumference and ankle circumference was smaller than the other circumference. In conclusion, the dimension combinations of the leg guard must be set differently to provide a leg guard with high size suitability according to leg type.

Electrochemical Properties for the Corrosion of Zinc Anode with Different Particle Size and Shape in Zinc/air Batteries (입자의 크기가 다른 아연공기전지용 아연음극의 부식에 관한 전기화학적 특성 연구)

  • Yang, Won-Geun;Hong, Jung-Eui;Oh, Rye-Gyeong;Oh, Ji-Woo;Kong, Young-Min;Ryu, Kwang-Sun
    • Journal of Powder Materials
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    • v.20 no.3
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    • pp.186-190
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    • 2013
  • The electrochemical performance for the corrosion of zinc anodes according to particle size and shape as anode in Zn/air batteries was study. We prepared five samples of Zn powder with different particle size and morphology. For analysis the particle size of theme, we measured particle size analysis (PSA). As the result, sample (e) had smaller particle size with $10.334{\mu}m$ than others. For measuring the electrochemical performance of them, we measured the cyclic voltammetry and linear polarization in three electrode system (half-cell). For measuring the morphology change of them before and after cyclic voltammetry, we measured Field Emission Scanning Electron Microscope (FE-SEM). From the cyclic voltammetry, as the zinc powder had small size, we knew that it had large diffusion coefficient. From the linear polarization, as the zinc powder had small size, it was a good state with high polarization resistance as anode in Zn/air batteries. From the SEM images, the particle size had increased due to the dendrite formation after cyclic voltammetry. Therefore, the sample (e) with small size would have the best electrochemical performance between these samples.

Development of Asbestos Quality Control Sample for Proficiency Analytical Testing 1 - Development of Manufacturing Apparatus and Sample Preparing Procedure for Asbestos Quality Control Sample - (석면분석 정도관리용 표준시료 개발연구 I - 석면분석 정도관리용 표준시료 제조장치 개발 및 시료제조 방법 확립 -)

  • Yi, Gwang Yong;Lee, Jong- Han;Jung, Sijeong;Park, Doo Yong
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.19 no.2
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    • pp.81-87
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    • 2009
  • Final purpose of this study was designed to develop the quality control(QC) sample for proficiency analytical testing of asbestos. This study consisted of two parts; first, development of manufacturing apparatus and sample preparing procedure for asbestos quality control(QC) sample: second, validation of the QC samples made by our developed method as asbestos proficiency analytical testing sample. The main results of the first part research are as followed We developed the apparatus for manufacturing the asbestos QC sample, consisted of filter hold, filter holder manifolder, vacuum system, and vacuum pump. The most proper filter of making the QC samples was a cellulose ester membrane filter with 25 mm diameter, pore size 0.8 um. And we presented the optimal procedure for preparing the asbestos QC sample by using the developed apparatus. We will verify the manufactured asbestos QC samples by this method, and present the validation results to confirm the reliability as a asbestos QC sample in next paper.

A Study on the Estimation of Convenient Sample Size for Cocoon Reeling Test (조사검정에 있어서의 적정시료량의 추정에 관한 연구)

  • 이인전
    • Journal of Sericultural and Entomological Science
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    • v.15 no.1
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    • pp.27-30
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    • 1973
  • The present research has been carried out in an attempt to obtain fundamental data which contribute to enhansing the accuracy of cocoon reeling test and improving the method of it more effectively. The results obtained are as follows: 1. The standard deviation and coefficient of variation of percentage of reelability and of percentage of raw silk yield to the sample size tended to show a gradual decrease according to the increasing of the sample size. However, the significant differences in the standard deviation and coefficient of variation of percentage of reelability and of percentage of raw silk yield between the different sizes of the samples having more than 300 cocoons have not been observed according to their sizes. 2. The significant difference in the standard errors from percentage of reelability between the samples of 300 cocoons and 400 cocoons have not been observed. 3. The significant difference in the standad errors from percentage of raw silk yield between the samples of 300 cocoons, 500 cocoons and 600 cocoons have not been observed. 4. The significant difference in the standard deviation, the standard errors and coefficient of variation to the length of a bave between the different sizes of the samples have not been shown. 5. From the above result, it is estimated that the optimum sire of the sample for the cocoon reeling test is the 300 cocoons.

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