• Title/Summary/Keyword: Salmonella paratyphi

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Antimicrobial Drug Resistance and R-plasmid of Salmonella species (Salmonella 균속의 항균제 내성 및 R-plasmid)

  • Lee Myung-Won;Chung Tae-Wha;Lee Yun-Tai;Kang Jeung-bok
    • Journal of environmental and Sanitary engineering
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    • v.3 no.2 s.5
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    • pp.23-41
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    • 1988
  • Two hundred and eighty-six strains of Salmonella species were isolated from the twelve provincial institutes of health and 19 general hospitals of urban and rural areas in Korea from January to December in 1986. The antimicrobial susceptibility test of these cultures was done by the method of agar diluton. The resistance frequency of Salmonella cultures was $29.7\%$. Among these resistant cultures, the most provalent resistance pattern of Salmonella was ampicillin, carbenicillin, chloramphenicol, tetracycline, streptomycin, and its resistance frequency was $15\%$. In plasmid profile of resistance strains, average number of plasmid harboring in Salmonella was 1-4 and molecular weight of plasmid ranged 1.6 to 70 megadalton (Md.). Plasmid pattern of strains isolated from Seoul and Kang-won showed the same or similar profiles. Plasmid pattern was identical in the same resistance pattern.

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A Study on the Insecticidal and Antibacterial Activity of the Carbamate derivatives (Carbamate 화합물의 殺蟲效果 및 抗菌力에 관한 연구)

  • Kang, Hoe-Yang;Jung, Chan
    • Journal of Environmental Health Sciences
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    • v.23 no.1
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    • pp.62-65
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    • 1997
  • The insecticidal and antibacterial activity of new synthesized carbamate derivatives(5,7-dibromo-8-hydroxyquinolinyl-N-methylcarbamate(I), 5,7-dibromo-8-hydroxyquinolinyl-N-ethylcarbamate(II)) was examined using 0.2w/v% acetone solutions and 50 $\mu$g/ml-1000 $\mu$g/ml N,N'-dimethylformamide H$_2$O(2:3) solutions of each compounds, respectively. 1. Two carbamates exerted insectiddal effects on Sogata furcifera HORVATH, Delphacodes Striatella FAUEN and Nilaparvate lugens STAL, Whereas no significant effects were observed on the Inazuma dorasalis MOISCHIULSKY Nephateffix apicalis Cincticeps UHLER. 2. These compounds exhibited growth-inhibitory activity against Staphyloccus aureus, Salmonella paratyphi A, Shigella dysenteriae 1a, Escherichia coli NL 1401, at the concentration range of 100-500 $\mu$g/ml in general.

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A Study on the Insecticidal and Antibacterial Activity of the 8-Hydroxy-5-chloroquinolyl Carbamates (8-Hydroxy-5-chloroquinolyl Carbamates의 살충효과 및 항균력에 관한 연구)

  • 강회양
    • Journal of Environmental Health Sciences
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    • v.21 no.1
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    • pp.74-77
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    • 1995
  • The insecticidal and antibacterial activity of new synthesized carbamate derivatives(8-Hydroxy-5-chloroquinolyl-N-methylcarbamate(I), 8-Hydroxy-5-chloroquinolyl-N-ethylcarbamate(II)) was examined using 0.2 w/v% acetone solutiolas and 50 r/ml~1000 r/m N,N'-dimethylformamide-$H_2O$(2:3) solutions of each compounds, respectively. 1) Two carbamates exerted insecticidal effects on Sogata furcifera HORVATH, Delphacodes Striatella FAUEN, Whereas no significant effects were observed on the Nilaparvate lugens STAL, Inazuma dorasails MOISCHIULSKY and Nephateffix apicalis Cincticeps UHLER. 2) These compounds exhibited growth-inhibitory activity against Staphylococcus aureus, Salmonella paratyphi A, Shigella dysenteriae 1a, Escherichia coli NL 1401,at the concentration range of 100~500 r/ml in general.

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Study on Enteric Pathogenic Bacteria from the Environments and Human (자연환경내에 오염된 식중독 원인균의 분포)

  • Lee, Yun-Tai;Lee, Chong-Hoon
    • The Journal of the Korean Society for Microbiology
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    • v.16 no.1
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    • pp.13-18
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    • 1981
  • Total 984 specimens were collected from sweages(130 specimens) and human rectal swabs(854 specimens) during period from October 1979 to November 1980 in Korea. Sixteen strains of salmonella were isolated from the human rectal swabs at the St. Mary's Hospital in Dae Jeon. They were confirmed into fifteen strains of S. typhi, one strain of S. paratyphi A. In other hand, fourty three strains of Shigella had been isolated from the stool specimens. They were typed as thirty four strains of Shigella flexneri, five strains of Shigella sonnei and one strain of Shigella dysenteriae. However, 16 strains of Vibrio eltor had been isolated from patients and carriers in the first epidemic areas in Chunla Namdo in September 1980. The serotype of isolated strains was determined as a Inaba which was resistant to the Mukerjee phage group IV but was susceptible to El Tor phage groupe I and II. Authors had been concluded that the strains were certain to be identified with Vibrio cholerae biotype El Tor.

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The Pathogenic Role of Enterobacteria(Salmonella, Shigella and E. coli spp) Isolated in Korea (한국에서 분리된 장내세균(Salmonella, Shigella, E. coli 균속)의 병원적 역할에 관한 연구)

  • Jung, Tae-Hwoa;Lee, Yun-Tai;Lee, Myung-Won;Lee, Bok-Kwon;Kim, Ki-Sang
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.1
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    • pp.73-95
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    • 1986
  • A total of 5,462 isolates suspicious of Salmonella, Shigella and E. coli which were isolated during 1983 to 1985 by 12 City Hygine Laboratories and General Hospital Laboratories were received and identified at the National Salmonella Center, Seoul, Korea. The result of identification of these strains were summarized as follows: 1. It was confirmed that the total organisms broke down into 2,014 strains of Salmonella 1,294 of which were S. typhi, 887 strains of Shigella and 2,561 strains of E. coli. 2. For seasonal distribution of enteric pathogens, July was the month with the highest out breaks of salmonellosis, May was the month of Shigellosis, and April was of the highest month it in the case of E. coli. 3. Salmonella typhi with the highest incidence of isolation was shown to belong to various phage types, especially with the strains detected in Seoul. M1 type was widely distributed all over the country, but the majority was E1 type in 1983. 4. For age distribution of patients, the 20-29 age group had the highest incidence of salmonellosis whileas the 1 to 9 age group had the highest incidence of Shigellosis. 5. For sexuly distribution of Salmonella and Shigella infections seemed to be relatively higher in the female than in the male. However, E. coli. had no relationship to both sex. 6. The antibiotic sensitivity patterns of S. typhi cultures showed a tendancy to be resistant to colistin, gentamycin, neomycin, tetracycline and streptomycin. 7. The isolates of S. paratyphi-A, S. typhimurium and S. enteritidis seemed to have a tendency of multiple drug resistance. 8. 93.9 percent of 1,568 E. coli strains showed negative reactions to the antisera of enteropathogenic E. coli and 15.6 percent of them produced a heat-labile enterotoxin, but positive reaction to the antisera was 6.1 percent and 11.6 percent of them producled the enterotoxin.

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An analysis of the enteropathogenic bacteria isolation during the last five years (최근 5년간 분리된 enteropathogenic bacteria)

  • Chong, Yun-Sop;Song, Kyung-Soon;Yi, Kui-Nyung;Lee, Sam-Uel Y.
    • The Journal of the Korean Society for Microbiology
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    • v.14 no.1
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    • pp.17-25
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    • 1979
  • Bacteriologic diagnosis of enteric infection remains to be an important role of clinical laboratory because of the prevalence of the infection. Often the determination of etiologic agent and its susceptibility to antibiotics are of vital importance for a proper management of the infection. In our previous paper, an analysis of the isolation of enteric pathogens for the years 1969-73 was reported to clarify the status of those years. The present analysis was made based on the data obtained during the years 1974-78, to see if any change of the status was rendered. 1. During the 5-year period, from the cultures of 7,308 stool or rectal specimens 833 patients yielded enteric pathogens: 468 Shigella, 295 Salmonella, 30 Vibrio parahaemolyticus and 40 enteropathogenic Escherichia coli(EPEC). 2. Of the 295 Salmonella, 271 were S. typhi Isolation of 12 S.paratyphi-A, 1 Salmonella group B, 4 group C, 5 group D and 2 group E meant a definite increase of these sero-groups, S. typhi was most frequently isolated in August and in December, and from 30- to 39-year-old patients. 3. Of the 468 Shigella, 10 were subgroup A, 338 subgroup B, 3 subgroup C and 117 subgroup D. Most of the subgroup B belonged to type 1,2, or 3. The proportion of S. sonnei decreased from 31.3% in 1974 to 18.2% in 1978. In foreign patients, S. sonnei remained to be the frequntly isolated species. Shigella isolation was frequent in August and in 2- to 5-year-old patients. 4. V. parahaemolyticus was isolated from 30 and EPEC from 40 patients. 5. Ninty-nine per cent and 99.5% of the S. typhi isolates were susceptible to chloramphenicol and to ampicillin respectively. 92.8% of S sonnei were susceptible to ampicillin. S. flexneri type 2 was notable for their markedely decreased proportion being susceptible to ampicillin: 84.4% in 1974 and 25.6% in 1978.

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Studies on the Distribution of Salmonella Group Organisms in Animals (1st. Report) Distribution of Salmonella Group Organisms in Dogs of Taegu Area (동물(動物)의 Salmonella속균(屬菌) 분포(分布)에 관(關)한 연구(硏究) 제(第)1보(報) 대구지역(大邱地域)의 견(犬)에 있어서의 Salmonella 속균(屬菌)의 분포(分布))

  • Jo, Han-Cheol
    • Korean Journal of Veterinary Research
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    • v.1 no.1
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    • pp.29-29
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    • 1961
  • 동물(動物)의 Paratyphoid의 원인균(原因菌)으로서 각종동물(各種動物)에서 여러학자(學者)들에 의(依)하여 많은 Salmonella속균(屬菌)이 분리보고(分離報告)되고 있으나 우리나라에서는 아직 동물(動物)에 있어서 Salmonella속균(屬菌)에 대(對)한 관심(關心)이 극(極)히 희박(稀薄)하여 돈(豚)의 Paratyphoid와 추백리(雛白痢)를 제외(除外)한 그 외(外)의 가축(家畜)의 Salmonella증(症)은 거이 간과(看過)되고 있는 상태(狀態)에 있으며 인간(人間)의 Paratyphoid 및 식중독등(食中毒等)에 밀접(密接) 관계(關係)가 있음을 생각할 때 가축(家畜)의 Salmonella증(症)은 단지 수의학(獸醫學) 뿐만 아니라 공중위생상(公衆衛生上) 경시(輕視)못할 문제(問題)이다. 특(特)히 견(犬)은 애완용(愛玩用), 수렵용(狩獵用), 또는 가정(家庭) 경비용등(警備用等)으로 인간생활(人間生活)에 있어 접촉(接觸)하는 기회(機會)가 가장 많은 동물(動物)이므로 이로 인(因)한 인간(人間)의 폐해(幣害)을 생각하지 않을수 없다. 필자(筆者)는 견(犬)에 있어서의 Salmonella속균(屬菌)의 분포상태(分布狀態)를 조사(調査)할 목적(目的)으로 1958년(年) 하계(夏季) 대구(大邱) 시내(市內) 광견병(狂犬病) 예방접종시(豫防接種時) 가정상양견(家庭飼養犬) 100두(頭)의 분변(奮便)을 검사(檢査)하여 6두(頭)에서 Salmonella속균(屬菌) 각(各) 1주(株)씩을 분리(分離)하였으며 다음과 같이 균형(菌型)을 결정(決定)하였다. Sal. typhi-murium 1주(株) Sal. cholerae-suis 1주(株) Sal. paratyphi A 1주(株) Sal. bartord 1주(株) 균형미결정(菌型未決定) 2주(株) (경북대학교(慶北大學校) 논문집(論文集) 제3집(第三輯)에 발표(發表)하였음).

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Effects of Plant-origin Biological Active Materials on the Activities of Pathogenic Microbes and Rumen Microbes (식물유래 생리활성물질의 병원성 미생물 및 반추위 미생물 활성에 대한 영향)

  • 옥지운;이상민;임정화;이신자;문여황;이성실
    • Journal of Animal Science and Technology
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    • v.48 no.5
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    • pp.709-718
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    • 2006
  • In order to know the effects of Garlic, Scallion, Flavonoid, Urushiol, Anthocyanidin and Bio-MOS?? on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro rumen microbial growth, gas production, ammonia concentration, carboxymethylcellulase(CMCase) activity, and microbial populations were investigated.The growth of pathogens was inhibited by supplementation of 0.1% Flavonoid, Scallion or Bio-MOS?? as biological active materials. And Scallion and Flavonoid had powerful antimicrobial properties on the pathogens applied in paper disc method.Although few effects by biological active materials disappeared in rumen fermentation in vitro, CMCase activity removed with supplementation of 1% of Flavonoid which had antimicrobial property in paper disc method. Scallion, having powerful antimicrobial property on pathogens and no inhibiting on rumen fermentation, might be a source in development of natural antimicrobial agent for ruminants.

Effects of Biologically Active Materials Prepared for Several Minerals and Plants on the Growth of Rumen Microbes (무기물성 및 식물성 생리활성 물질이 반추위 미생물의 성장에 미치는 영향)

  • Shin, Sung-Whan;Lee, Shin-Ja;Ok, Ji-Un;Lee, Sang-Min;Lim, Jung-Hwa;Kim, Kyoung-Hoon;Moon, Yea-Hwang;Lee, Sung-Sill
    • Journal of Life Science
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    • v.17 no.11
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    • pp.1555-1561
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    • 2007
  • In order to know the effects of scoria, germanium, charcoal, ginger, stevia, and CLA(Conjugated Linoleic Acid) as biologically active materials on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro lumen microbial growth, gas production, ammonia concentration, carboxymethyl-cellulase (CMCase) activity, and microbial populations were investigated. The growth of pathogenic microbes was inhibited by the supplement of 0.10% ginger. Ginger had powerful antimicrobial properties on all the pathogens used in this experiments. Additionally in the antibacterial assay by paper disc method, we could observe the clear zone of similar area with the positive control(antibiotics) for E. coli as applied with the 10% stevia or the 10% CLA only. The supplements of ginger, stevia and CLA in vitro rumen fermentation inhibited populations of rumen bacteria and protozoa. Particularly supplement of ginger resulted in remarkable reduction of the protozoa population, which means it might serve as a source inhibiting material of methane creation in the rumen.

Antibacterial, Anti-Diarrhoeal, Analgesic, Cytotoxic Activities, and GC-MS Profiling of Sonneratia apetala (Buch.-Ham.) Seed

  • Hossain, Sheikh Julfikar;Islam, M Rabiul;Pervin, Tahmina;Iftekharuzzaman, M;Hamdi, Omer AA;Mubassara, Sanzida;Saifuzzaman, M;Shilpi, Jamil Ahmad
    • Preventive Nutrition and Food Science
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    • v.22 no.3
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    • pp.157-165
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    • 2017
  • Fruits of Sonneratia apetala (Buch.-Ham.), (English: mangrove apple, Bengali: keora) both seeds and pericarps, are largely consumed as food besides their enormous medicinal application. The fruit seeds have high content of nutrients and bioactive components. The seeds powder of S. apetala was successively fractionated using n-hexane, diethyl ether, chloroform, ethyl acetate, and methanol. The fractions were used to evaluate antibacterial, anti-diarrhoeal, analgesic, and cytotoxic activities. Methanol fraction of seeds (MeS) stronly inhibited Escherichia coli strains, Salmonella Paratyphi A, Salmonella Typhi, Shigella dysenteriae, and Staphylococcus aureus except Vibrio cholerae at $500{\mu}g/disc$. All the fractions strongly inhibited castor oil induced diarrhoeal episodes and onset time in mice at 500 mg extract/kg body weight (P<0.001). At the same concentration, MeS had the strongest inhibitory activity on diarrhoeal episodes, whereas the n-hexane fraction (HS) significantly (P<0.05) prolonged diarrhoeal onset time as compared to positive control. Similarly, HS (P<0.005) inhibited acetic acid induced writhing in mice at 500 mg extract/kg, more than any other fraction. HS and diethyl ether fractions of seed strongly increased reaction time of mice in hot plate test at 500 mg extract/kg. All the fractions showed strong cytotoxic effects in brine shrimp lethality tests. Gas chromatography-mass spectrometry analysis of HS led to the identification of 23 compounds. Linoleic acid (29.9%), palmitic acid (23.2%), ascorbyl palmitate (21.2%), and stearic acid (10.5%) were the major compounds in HS. These results suggest that seeds of S. apetala could be of great use as nutraceuticals.