• Title/Summary/Keyword: Salivary gland

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IMMUNOHISTOCHEMICAL ASSAYS FOR THE EXPRESSION OF EPIDERMAL GROWTH FACTOR-SIGNALING PROTEINS IN ADENOID CYSTIC CARCINOMAS OF HUMAN SALIVARY GLANDS (타액선 선양낭성암종에서 상피성장인자 신호전달 단백의 발현에 관한 면역조직화학적 연구)

  • Park, Young-Wook;Kim, Jung-Hwan
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.28 no.6
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    • pp.499-510
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    • 2006
  • Malignant tumors of the human salivary glands may arise from major or minor salivary glands. Adenoid cystic carcinoma (ACC) is the second most common malignant neoplasm in the salivary glands. ACC is occasionally highly aggressive tumor that readily invades adjacent tissues and metastasize to distant organs at early stages of the disease. Although ACC tends to grow slowly, treatment outcome may be poor due to wide local infiltration, perineural or intraneural spread and a propensity for hematogenous metastasis. Therefore, knowledge of cellular and molecular characteristics that influence the growth, survival and metastasis of tumor cells, is important for new treatment strategies of salivary ACC. I determined expressions of epiderma growth factor (EGF)-signaling molecules using surgical specimens of human ACCs. Protein expressions of EGF, transforming growth $factor(TGF)-{\alpha}$, EGF receptor (EGFR), phosphorylated EGFR (pEGFR), and human EGF receptor (HER)-2 were assessed in 18 cases of salivary ACC by immunohistochemical staining. Adjacent normal salivary tissues and mucosal tissues, uninvolved by the malignant tumor, served as internal controls. Most of the tumors, especially ACC with a tubulocribriform pattern, were positive for EGF signaling molecules. The overall percentages of the 18 specimens expressing EGF, $TGF-{\alpha}$, EGFR, pEGFR, and HER2 were 50, 89, 61, 61 and 83% respectively. Moreover, tumor-associated endothelial cells and infiltrating immune-related cells in the stroma of ACC, also expressed these biomarkers. Taken together, EGF-signaling molecules are actively expressed in salivary ACC. Therefore, we suggest that these biomarkers can be molecular targets for new treatment strategies of salivary tumors.

AN EXPERIMENTAL STUDY OF THE IRRADIATION EFFECTS ON RAT PAROTID GLAND (방사선조사가 백서 이하선에 미치는 영향에 관한 실험적 연구)

  • Lee Kyu Chan;Lee Sang Rae
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.16 no.1
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    • pp.49-58
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    • 1986
  • This study was designed to investigate the irradiation effects on the rat parotid gland, applied to the head and neck region. For this experiment, twenty-four rats, feeded under the even condition, were used as experimental animals. Twenty rats were used for experimental group and the rest were assigned to the control group. The experimental group was singly irradiated with 10Gray through Cobalt-60 radiotherapy device, Picker model 4M 60 (Field size; l2×5㎝, SSD; 50㎝, Depth; 1㎝). The experimental animals of both group were sacrificed each four animals in 2 days, 1week, 2weeks, 3weeks and 4weeks after irradiation. The specimens were examined through the light microscope using the H-E stain and H stain by routin procedure. The other specimens were observed under the fluorescence microscope using the B-O dichroic mirror and Y455 barrier filter after PA-ACH stain. 1. The results of this study were obtained as follows, The parotid acini were severely degenerated and the intraacinar spaces were widened. Within the acini, retained secretory granules and increased fibrosis were observed. Also the shape and the size of the acini showed very irregular atrophic degenerations. 2. The nuclei showed severe pyknosis, displacement and irregular aggregated appearance. 3. The tissue changes of the parotid acini were initiated after 2 days of irradiation and most severely appeared at the second week of irradiation, but almost returned to normal. 4. The salivary ducts of the parotid gland were severely atrophied, discontinued but initiated to regenerated after 3 weeks of irradiation.

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Primary Adenoid Cystic Carcinoma of Salivary Gland with Metastatic to the Lung (악하선 종괴를 동반한 고립성 폐결절)

  • Cho, Hai-Jeong;Kim, Jin-Hee;Kim, Ju-Ock;Song, Kju-Sang;Nam, Boo-Hyun;Lim, Seung-Pyung;Kim, Sun-Young
    • Tuberculosis and Respiratory Diseases
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    • v.41 no.5
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    • pp.579-583
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    • 1994
  • A 22-year old female visited CNUH due to palpable neck mass. Cytologic examination of a fine needle aspiration was performed and the result was Pap class II. Routine chest x-ray shows solitary pulmonary nodule. For rule-out malignancy, FNA at neck mass was repeated and pathologic finding was dysplasia. She was admitted to MI department for evaluation of solitary pulmonary nodule and percutaneous needle aspiration was done. Pathologic diagnosis was adenoid cystic carcinoma. Thereafter, the lesions were treated by excisional biopsy of submandibular gland mass with left supraomohyoid neck dissection and wedge resection of right lower lobe at ENT department and thoracic and cardiovascular surgery department, respectively. Final diagnosis was adenoid cystic carcinoma arising in submandibular gland with solitary lung metastasis. According to TMN staging system, surgical staging is stage IV of T2N0M1. Clinical follow-up to postoperative 13 months in this case showed that she is alive and well without evidence of recurrence.

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A Case of Bilateral Pneumoparotid Improved with Conservative Treatment (보존적 치료로 호전된 양측 이하선 기종 1예)

  • Oh, Yun Seok;Kim, Jeong Marn;Jung, Hahn Jin;Shim, Woo Sub
    • Korean Journal of Head & Neck Oncology
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    • v.33 no.1
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    • pp.43-45
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    • 2017
  • Pneumoparotid is defined as the presence of air within the parotid gland or duct. Pneumoparotid is an extremely rare cause of parotid gland swelling. Pneumoparotid can be induced by air insufflation from oral cavity into the intraparotid salivary ducts, in condition like nose blowing, blowing up balloons, during extubation after general anesthesia while receiving positive pressure, rapid decompression while scuba diving, chronic attempts to suppress cough, and glassblowers. It can also be self-induced, and the literature contains an increasing number of reports involving pediatric and adolescent patients who induce parotid insufflation to avoid school or gain attention. Here, we report a case of pneumoparotid caused by habitual cheek inflating and improved with conservative treatment.

The Effects of Diltiazem and Pentoxifylline on Apoptosis of Irradiated Rat Salivary Gland (흰쥐 침샘의 방사선조사시 Apoptosis에 대한 Diltiazem과 Pentoxifylline의 효과)

  • Yang, Kwang-Mo;Suh, Hyun-Suk
    • Radiation Oncology Journal
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    • v.16 no.4
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    • pp.367-375
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    • 1998
  • Purpose : Xerostomia is a complication met by almost all patients who have radiotherapy for cancers of head and neck. Many studies for prevention of xerostomia will be necessary. Radiation-induced acute response of salivary glands has been defined as interphase death or apoptosis. Increased intracellular calcium level have an important role in radiation-induced apoptosis. Calcium channel blocker may prevent radiation-induced apoptosis of salivary glands. This study was designed to evaluate the effectiveness of diltiazem known as calcium channel blocker and pentoxifylline with inhibition of inflammatory response on the apoptosis as an acute response of radiation in rat salivary glands. Materials and Methods : Sprague-Dawley rats with about body weight 200-250 g were divided into 5 study groups : control, radiation alone, diltiazem with radiation, pentoxifylline with radiation, and diltiazem and pentoxifylline with radiation. The diltiazen and pentoxifylline were injected intraperitoneally 20 mg/kg and 50 mg/kg, 30 and 20 mimute before irradiation. respectively. Irradiation was given with a 4 MV linear accelerator. The 1600 cGy of radiation was delivered in a single fraction through a single anterior portal encompassing the entire neck. After 24 h of irradiation, rats were sacrificed and parotid and submandibular glands were removed and stained with hematoxylin and eosin. The quantification of apoptosis was performed by microscopic examination of stained tissue sections at a magnification of 200X and the percentage of apoptotic cell was calculated. Results : On parotid glands, the percentage of apoptosis by radiation alone, diltiazem with radiation, pentoxifylline with radiation, and diltiazem and pentoxifylline with radiation were 1.72$\%$ (8.35/486), 0.64$\%$ (2.9/453), 0.23$\%$ (1.2/516), and 0.28$\%$ (1.1/399), respectively. The apoptosis was markedly reduced in the groups receiving drugs compared with groups receivinge, radiation alone (p<0.05). In serous cell of submandibular glands, the percentages of apoptosis of radiation alone, diltiazem with radiation, pentoxifylline with radiation, and diltiazem and pentoxifylline with radiation were 1.94$\%$ (l1/567), 0.34$\%$ (1.9/554), 0.28$\%$ (1.8/637), and 0.22$\%$ (1.3/601), respectively. In the mucus cell of submandibular glands, the percentages of apoptosis were 0.92$\%$ (5.1/552), 0.41$\%$ (2.5/612), 0.29$\%$ (1.3/455), and 0.18$\%$ (1.0/562), respectively. The apoptosis was markedly reduced in the serous glands (p<0.05), but there was no difference in development of apoptosis in each group of mucus gland. Conclusion : These results suggest that radiation-induced apoptosis of serous cells of salivary glands may be decreased by diltiazem and pentoxifylline administration.

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Ultrastructure of the Submandibular Gland in the Big White-Toothed Shrew, Crocidura lasiura (우수리땃쥐 Crocidura lasiura 악하선의 미세구조)

  • Jeong, Soon-Jeong;Lim, Do-Seun;Park, Joo-Cheol;Kim, Heung-Joong;Jeong, Je-O;Choi, Baik-Dong;Yoon, Myung-Hee;Jeong, Moon-Jin
    • Applied Microscopy
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    • v.35 no.2
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    • pp.57-64
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    • 2005
  • The ultrastructure and histochemical characteristics of the submandibular gland was examined in the big white-toothed shrew, Crocidura lasiura. A submandibular gland of Crocidura lasiura was a mixed gland composed of serous and mucous acinar cells. Secretory granules from the acini were discharged through the intercalated duct, the granular duct and the striated duct into the oral cavity. Serous and mucous acinar cells and granular duct cells had large amount of rough endoplasmic reticulum, free ribosome and prominent Golgi apparatus at the basal cytoplasm of the cell, and many granules at the apical cytoplasm. Oval type serous granules had a homogeneously pale round shape of bead at the center. Mucous granules were distinct from those of the other mammalian species having variety patterns with several dense bands into homogeneous pale matrix. A serous-like secretory granules and myelin-like body were observed in the cytoplasm and the lumen of granular duct cells. The myelin-like body is a characteristic structure only reported in the salivary glands of two shrews, Suncus murinus and C. dsinezumi. Striated duct cell had numerous well-developed mitochondria but secretory granule was not shown at all.

방사선조사가 백서타액선조직에 미치는 영향에 관한 실험적 연구

  • Lee, Eui-Wung
    • The Journal of the Korean dental association
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    • v.18 no.8 s.137
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    • pp.657-667
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    • 1980
  • This study was undertaken to observe the salivary gland of the white rat when exposed to single and fractionated doses of Cobalt-60 irradiation. One hundred fifty white rats of the experiment were devided into control and 3 experimental groups. In experimental groups, group I receivcd 1200 rads everyweek untill 4800 rads reached, group II received 1500 rads and group III received 2000 rads with single dose. irradiation was carried out using a RAC-120 Cobalt-60 Teletherapy Unit with a dose rate 84.3 r/min, field size 4×5 cm measured at 80 from source. Rats were serially sacrificed at the following postirradiation time intervals: 1, 3, 5, 7, 10, 14, 21, 28, 35, and 42days. At sacrifice, the parotid and submandibular glands were dissected out in toto, and stained with: 1) hematoxylin and eosin; 2) periodic acid Shiff; 3) toludine blue.

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A Repetitive Secretory Protein Gene of A Novel Type in Hydropsyche sp. Is Specially Expressed in the Silk Gland

  • Eun, Jai-Hoon;Goo, Tae-Won;Park, Kwang-Ho;Yun, Eun-Young;Hwang, Jae-Sam;Kang, Seok-Woo;Han, Sung-Sik
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.153-154
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    • 2003
  • Trichoptera, or caddisflies, comprise one of the major aquatic insect orders. Like Lepidoptera, caddisflies are capable of spinning silk from specially modified salivary glands, and the diversity of ways this silk is used probably accounts for the success of the order as a whole. These utilize silk to construct both larval and pupal shelters, often incorporating materials from the environmental among the silk thread. In this study, we try to find and characterize novel type genes that should be translated to major component protein of aquatic silk. (omitted)

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A CASE REPORT OF PLEOMORPHIC ADENOMA OF BUCCAL MUSOCA (협점막에 발생한 혼합선종의 치험예)

  • Heo, Cheol;Kim, Jin-Ho;Lee, Chung-Guk;Sin, Jong-Ho
    • The Journal of the Korean dental association
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    • v.14 no.8
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    • pp.679-682
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    • 1976
  • This 32 year-old male army officer was admitted with complaints of thumb size mass on buccal musoca which onterfere with mastication. Routine laboratory test revealed W.N.L. and the results of the excisional biopsy was Plemorphic Adenoma of salivary gland origin which was on the left buccal musoca( of 21 year-old male army officer). The size of tumor mass was 1.0 x 1.2 x 0.8cm, hard, encapsulated and seperated completely from surrounding tissue when enucleated surgically. Wound was healed unventfully and the result was good.

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Imaging Diagnosis in Salivary Gland Tumors (타액선 종양의 영상진단)

  • Han Moon-Hee
    • Korean Journal of Head & Neck Oncology
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    • v.9 no.1
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    • pp.88-90
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    • 1993
  • 병변의 양상에 따른 감별진단 재발성 이하선 종대를 보이는 경우 타액선조영술이 영상진단방법중 1차적인 선택이 된다. Stensen 씨관이나 중심선관(central glandular duct)의 확장을 보이는 경우 만성 타액선염으로 진단할 수 있다. 소아에서의 단일성 양성종괴의 감별진단은 임파절, 혈관종, 양성혼합종양, 저급점액상피암, 임파관종 등이다. 성인에서의 단일성, 양성으로 관찰되는 종괴는 양성혼합종양, Warthin씨 종양, 저급점액상피암, 선상낭성암, 소포상세포암 등이다. 여러개의 종괴를 보이는 경우 Warthin씨 종양, 소포상세포암, 임파종, 육아종, 전이암 등이며 단일성 낭성종괴의 경우는 branchial cleft cyst,, Warthin씨 종양, 상피낭포 등이다.

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