• Korean Journal of Veterinary Research
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• v.41 no.3
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• pp.319-325
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• 2001

Several recent studies demonstrate that cAMP accumulation evokes marked changes in magnesium ($Mg^{2+}$) homeostasis. The goal of this study was to investigate the effect of melatonin, the principal hormone of the vertebral pineal gland, on $Mg^{2+}$ regulation in perfused guinea pig hearts. We hypothesized that melationin would regulate $Mg^{2+}$ efflux induced by adrenergic drugs and cAMP analogues because melatonin inhibites adneylate cyclase (AC) and phospholipase C(PLC) in the hearts. The $Mg^{2+}$ content in the perfusate was significantly higher in the presence than in the absence of melatonin. The addition of forskolin, isoproterenol or dimaprit to perfused hearts induced a marked $Mg^{2+}$ efflux. These effluxes were not inhibited by melatonin. The $Mg^{2+}$ efflux could also be induced by phenylephrine, a ${\alpha}_1$-adrenoceptor agonist. This phenylephrine-induced $Mg^{2+}$ efflux was inhibited by melatonin. In addition, the phenylephrine-induced $Mg^{2+}$ efflux was potentiated by PMA, a protein kinase C(PKC) activator. This $Mg^{2+}$ efflux was inhibited by melatonin. In conclusion, these data suggest that melatonin regulates $Mg^{2+}$ homeostasis and the inhibitory effect of melatonin on ${\alpha}_1$-adrenoceptor-stimulated $Mg^{2+}$ efflux may occur through an inhibition of PLC pathway in perfused guinea pig hearts.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.19 no.1
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• pp.203-209
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• 2019

In this work, a new land-specific resistance measuring device (GM) and a measuring probe (Grounding Rod) are connected to the WENNER quadrant as power-line communication (PLC). In groups of two (P1,P2) probes, five to ten probes are installed in series on the ground at intervals of 1m, 2m, 4m, 8m, and 16m, respectively. If the PLC signal from the GMD is detected by the receiver of the Probe 1 (P1) for measurement, the minute voltage and current for measurement flow from the PSD (power supply) attached to the probe to the ground, and then, through the soil between P1 and P2, enters the Probe 1 (P2). The resistance value is then measured by the principle of voltage drop due to ground resistance. Measure the earth resistance every T seconds up to 1 trillion and store the measured data on the Arduino Server mounted on the main equipment. Stored measurement data can be derived from formulas by Ohm's Law and from inherent resistance (here,). Data obtained in real time will be linked to CDGES programs installed on Main PC, enabling data analysis and real-time monitoring of the ground environment on land. In addition, a three-dimensional display is possible with 3D graph support by identifying seasonal characteristics such as temperature and humidity of land (soils). The limitations of the study will require specific application measures of Test Bed for commercial access to a model that has been developed and operated experimentally.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 1997.11a
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• pp.244-251
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• 1997

Since 1970s, The Petrochemical Industry in Korea has grown rapidly by the successful economic growth. While the process became larger and more complex, hazardous chemicals have been used in large quantity, Therefore, the risk of a major accident such as fire, explosion and toxic material release has been increased. Korea has been ranked the fifth in petrochemical product capacity worldwide, also required to meet international standards on process safety management. Fire Protection System integral part of safely management in Petrochemical Plants, will be reviewed.

• The Journal of the Korea institute of electronic communication sciences
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• v.13 no.6
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• pp.1229-1234
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• 2018

In this paper, a fire prevention system based on a broadband powerline communication (PLC) system is implemented and a demonstration experiment is carried out to prevent from or promptly dealing with possible fires within the nacelle of a wind turbine generator system (WTGS). For this purpose, an inductive coupler having satisfactory attenuation characteristic in the frequency region for high-speed PLC is also manufactured. It is confirmed that the implemented system can monitor the environmental change inside the nacelle in real time by transmitting various information obtained by the sensors such as temperature, flame, and smoke sensor installed in the nacelle and thermal image recorded by a thermal camera to the ground control center through the PLC system. Therefore, it is, considered that the implemented system will significantly improve the reliability of the fire monitoring and prevention system of the WTGS in conjunction with the existing safety system.

• Journal of Food Hygiene and Safety
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• v.23 no.3
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• pp.248-256
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• 2008

To investigate the epidemiological characteristics of 68 Listeria monocytogenes isolates, including 11 reference strains and 57 isolates from imported US beef, domestic meats(beef, pork, chicken meat), raw milk, and milk plants. L. monocytogenes was to evaluate the production of virulence proteins, such as hemolysin(LLO) and lecithinase(LCP), the adsorption of Congo red(CRA), and to detect virulence genes using the polymerase chain reaction(PCR). In the study of virulence protein production, 68(100%), 62(91.2%), and 54(79.4%) of the 68 L. monocytogenes strains were positive for LLO production, the LCP test, and the CRA test, respectively, while strains of other species, such as L. innocua, L. gray, L. murrayi, and L. welshimeri, were not. There were no significant differences between L. monocytogenes serotypes and the ability to produce LLO or LCP. L. monocytogenesstrains had very high hemolytic titers(2 to 16 fold), while the other Listeria species, other than L. ivanovii and L. seeligeri, did not. The hemolysin activities of L. monocytogenes, L. ivanovii, and L. seeligeri usually exceeded 1.0 HU/mg, while those of other Listeria spp. were less than 0.04 HU/mg. In the PCR assay, all of the L. monocytogenes strains contained the hlyA, plcA, plcB, inlA, and inlB virulence genes and produced a product of the expected size. In the PCR of the actA gene, the expected 385-bp product was seen in 39(57.4%) L. monocytogenesstrains, while an unexpected 268-bp product was seen in 29(42.6%) strains. Most L. monocytogenes strains isolated from Hanwoo beef produced the 385-bp actA gene product, while strains of imported US beef usually produced the 268-bp actA gene product. By contrast, no virulence gene products were amplified in the other Listeria spp.

• Nuclear Engineering and Technology
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• v.51 no.7
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• pp.1791-1798
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• 2019

Instrumentation and Control (I&C) systems of nuclear power plants (NPPs) have been continuously digitalized. These systems have a critical role in the operation of nuclear facilities by functioning as the brain of NPPs. In recent years, as cyber security threats to NPP systems have increased, regulatory and policy-related organizations around the world, including the International Atomic Energy Agency (IAEA), Nuclear Regulatory Commission (NRC) and Korea Institute of Nuclear Nonproliferation and Control (KINAC), have emphasized the importance of nuclear cyber security by publishing cyber security guidelines and recommending cyber security requirements for NPP facilities. As described in NRC Regulatory Guide (Reg) 5.71 and KINAC RS015, challenge response authentication should be applied to the critical digital I&C system of NPPs to satisfy the cyber security requirements. There have been no cases in which the most robust response authentication technology like challenge response has been developed and applied to nuclear I&C systems. This paper presents a challenge response authentication mechanism for a Programmable Logic Controller (PLC) system used as a control system in the safety system of the Advanced Power Reactor (APR) 1400 NPP.

• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.872-879
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• 2015

Many strains of Bacillus cereus cause gastrointestinal diseases, and the closely related insect pathogen Bacillus thuringiensis has also been involved in outbreaks of diarrhea. The diarrheal diseases are attributed to enterotoxins. Sixteen reference strains of B. cereus and nine commercial and 12 reference strains of B. thuringiensis were screened by PCR for the presence of 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, bceT, entFM, and entS), one emetogenic gene (ces), seven hemolytic genes (hlyA, hlyII, hlyIII, plcA, cerA, cerB, and cerO), and a pleiotropic transcriptional activator gene (plcR). These genes encode various enterotoxins and other virulence factors thought to play a role in infections of mammals. Amplicons were successfully generated from the strains of B. cereus and B. thuringiensis for each of these sequences, except the ces gene. Intriguingly, the majority of these B. cereus enterotoxin genes and other virulence factor genes appeared to be widespread among B. thuringiensis strains as well as B. cereus strains.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.11a
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• pp.229-232
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• 2000

This research aims at developing a low-cost PC-NC system based on one-CPU and investigating the feasibility of its application to a simple-function lathe. Its hardware consists a two axes motion control board including a 24bit counter, 8253 timer, a 12bit DA converter, DIO board for PLC operation and a PC with Intel Pentium 466MHz. The fundamental real-time MC functions such as G-code interpretation, interpolation, position and velocity control of axes are performed. User programming interface with functions of icon manipulation, tool-path simulation and NC-code generation was implemented. In order to achieve real-time control and safety, axis control, NC interpretation, interpolation and user communication are completely executed during every interrupt interval of I msec.

• Proceedings of the Korean Information Science Society Conference
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• 2006.06c
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• pp.220-222
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• 2006

본 논문은 안전등급 제어기기(Safety-Grade Programmable Logic Controller)에서 사용하는 프로세서모듈 운영체제에 대한 컴포넌트 시험에 대해 기술한다. 디지털 소프트웨어에 대한 NRC(Nuclear Regulatory Commission)의 지침에 따라 운영체제는 소프트웨어 생명주기에 따라 개발되고 있으며 요구사항과 설계명세, 그리고 구현코드를 가지고 다양한 시험을 수행하고 있다. 컴포넌트 시험은 구현된 코드가 테스트 커버리지를 만족하는 지 파악하는 시험이다. 이를 위해 설계명세서를 참조하여 시험대상을 구분하고 각각의 시험대상에 대한 시험항목을 세분화한 이후 시험방법과 절차, 그리고 시험환경을 구축한 후 컴포넌트 시험을 수행한다.

• Proceedings of the Safety Management and Science Conference
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• 2002.11a
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• pp.157-163
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• 2002

Automated manufacturing systems are applied to shop floors as a tool for the increase of productivity and quality and the decrease of manufacturing lead times and industry accidents. One of the most important issue of the present day is the application of Internet. The development of Internet technologies makes manufacturing enterprises break spacial barriers between users and shop floors, and collect various field data in remote sites. In this research, an Internet-based remote control system for a small-sized automated storage and retrieval system is developed for the purpose of real-time monitoring and control of automatic production equipment. The developed system has a client-server architecture and transmits real-time images of the automated storage and retrieval system to a client by ar CCD camera connected to a server. Based on the transmitted images, the client sends commands to PLC of the server, and part storage and retrieval tasks are executed.