• Design & Manufacturing
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• v.15 no.2
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• pp.11-16
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• 2021

Recently, three-dimensional (3D) cell culture systems, which are superior to conventional two-dimensional (2D) vascular systems that mimic the in vivo environment, are being actively studied to reproduce drug responses and cell differentiation in organisms. Conventional two-dimensional cell culture methods (scaffold-based and non-scaffold-based) have a limited cell growth rate because the culture cannot supply the culture medium as consistently as microvessels. To solve this problem, we would like to propose a 3D culture system with an environment similar to living cells by continuously supplying the culture medium to the bottom of the 3D cell support. The 3D culture system is a structure in which microvascular structures are combined under a scaffold (agar, collagen, etc.) where cells can settle and grow. First, we have manufactured molds for the formation of four types of microvessel-mimicking chips: width / height ①100 ㎛ / 100 ㎛, ②100 ㎛ / 50 ㎛, ③ 150 ㎛ / 100 ㎛, and ④ 200 ㎛ / 100 ㎛. By injection molding, four types of microfluidic chips were made with GPPS (general purpose polystyrene), and a 100㎛-thick PDMS (polydimethylsiloxane) film was attached to the top of each microfluidic chip. As a result of observing the flow of the culture medium in the microchannel, it was confirmed that when the aspect ratio (height/width) of the microchannel is 1.5 or more, the fluid flows from the inlet to the outlet without a backflow phenomenon. In addition, the culture efficiency experiments of colorectal cancer cells (SW490) were performed in a 3D culture system in which PDMS films with different pore diameters (1/25/45 ㎛) were combined on a microfluidic chip. As a result, it was found that the cell growth rate increased up to 1.3 times and the cell death rate decreased by 71% as a result of the 3D culture system having a hole membrane with a diameter of 10 ㎛ or more compared to the conventional commercial. Based on the results of this study, it is possible to expand and build various 3D cell culture systems that can maximize cell culture efficiency by cell type by adjusting the shape of the microchannel, the size of the film hole, and the flow rate of the inlet.

• The Korean Journal of Pesticide Science
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• v.11 no.4
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• pp.222-229
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• 2007

It is well known the functional foods are very useful to prevent serious diseases and promote health. Therefore, they are often called as nutraceuticals, designer foods, and pharmafoods, etc. Most of foods have diverse functions because they provide nutrients, energies and fibrinoid materials. When foods are taken in the body, they promote the biological defense system against diseases through the supply of the essential or healthy materials to human being's organs. The mode of action and functional foods in human body have not been clarified yet. Antioxidant is known as one of the therapeutic aids which can be reduced pesticide poisoning. Onion has a strong antioxidant effect. This study was carried out to elucidate an antioxidant function of onion by determination of superoxide dismutase in liver, lung, serum of male rat administered by intraperitonial injection of 0, 2, $4\;mg\;kg^{-1}$ cblorpyrifos after administrated orally with onion for 6 weeks. Damage of liver and kidney was also investigated by biochemical analysis of serum(AST, ALT, BUN/Creatine ratio). SOD(superoxide dismutase) activity of onion-administrated group is higher than control group. In liver and lung, SOD activity of onion+cblorpyrifos administrated group is higher than only chlorpyrifos administrated group. BUN/Creatinine ratio of onion+chlorpyrifos administrated group was decreased compared with only chlorpyrifos-administrated group.

• Fire Science and Engineering
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• v.27 no.2
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• pp.6-10
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• 2013

This study aims to construct the performance data for smoke-control zone and realize the safety of injection and pressurization room which is composed of supply air pressure zone, vestibule, smoke-control zone and stairwell. To obtain this, smoke-control system and the device of the opening-closing force of fire door are manufactured. This subject is the analysis of the closing force, angular velocity and fire door size in the case of fixed volume flow rate. Based on the results, closing force increased as fire door size and closing angular velocity increases. Also, it is remark that there exists a critical angular velocity, which maintains constant maximum closing force even though the angular velocity increases more.

• Nuclear Engineering and Technology
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• v.27 no.4
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• pp.470-482
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• 1995

The Ulchin 3&4, which are 2825 MWt PWRs, adopted Safety Depressurization System (SDS) to mitigate the beyond design basis event of Total Less of Feedwater(TLOFW). In this study the results and methodology of the analyses for the determination of SDS bleed capacity are discussed. The SDS design bleed capacity has been determined from the CEFLASH-4AS/REM simulation according to the following design criteria : 1) Each SDS flow path, in conjunction with one of two High Pressure Safety Injection (HPSI) pumps, is designed to have a sufficient capacity to prevent core uncovery if one SDS path is opened simultaneously with the opening of the Pressurizer Safety Valves (PSVs). 2) Both SDS bleed paths are designed to have sufficient total capacity with both HPSI pumps operating to prevent core uncovery if the Feed and Bleed (F&B) initiation is delayed up to thirty minutes from the time of the PSVs lift. To verify the results of CEFLASH-4AS/REM simulation a comparative analysis kas also been per-formed by more sophisticated computer code, RELAP5/MOD3. The TLOFW event without operator recovery and TLOFW event with F&B are analyzed. The predictions by the CEFLASH-4AS/REM of the transient too phase system behavior are in good qualitative and quantitative agreement with those by the RELAP5/MOD3 simulation. Both of the results of analyses by CEFLASH-4AS/REM and RELAP5/MOD3 have demonstrated that decay heat removal and core inventory make-up can be successfully accomplished by F&B operation during now event for the Ulchin 3&4.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.11 no.3
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• pp.119-126
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• 2017

In the clinical setting, the pressure bio-feedback device is used for the spinal rehabilitation of patients with back pain, but it has several disadvantages. The purpose of this study was to develop a digitalized pressure biofeedback system that provides precise exercise method and posture in real time during the spinal rehabilitation exercise by sensing and monitoring body movements and balance of users and providing biofeedback to users. After that, the usability testing for a digitalized pressure biofeedback system will be conducted to identify problems such as safety, performance, operability, and satisfaction, and suggest improvement directions. A total of 33 subjects were participated in the usability testing. The experts group and the users group evaluated the developed digitalized pressure biofeedback system on a scale of 5 points after using the equipment. In the user group, safety was 3.59, operability was 4.38, satisfaction was 4.49. In the expert group, safety was 2.86, operability was 3.91, and performance was 4.28. Based on the usability evaluation, if the problems of stability of the cradle for tablet PC, air injection, screen display, etc. are solved, it becomes a exercise device capable of accurately exercising and evaluating the function of the spine by checking its own motion state while the spinal stabilization exercise.

• Journal of IKEEE
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• v.25 no.4
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• pp.782-785
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• 2021

In this paper, we propose a personalized cooling management system with thermal imaging camera. The proposed equipment uses a thermal imaging camera to control the amount of cold air and the system according to the difference between the user's skin temperature before and after the procedure. When the skin temperature is abnormally low, the cold air supply is cut off to prevent the possibility of a safety accident. It is economical by replacing the skin temperature sensor with a thermal imaging camera temperature measurement, and it can be visualized because the temperature can be checked with the thermal image. In addition, the proposed equipment improves the sensitivity of the sensor that measures the distance to the skin by calculating the focal length by using a dual laser pointer for the safety of a personalized cooling management system to which a thermal imaging camera is applied. In order to evaluate the performance of the proposed equipment, it was tested in an externally accredited testing institute. The first measured temperature range was -100℃~-160℃, indicating a wider temperature range than -150~-160℃(cryo generation/USA), which is the highest level currently used in the field. In addition, the error was measured to be ±3.2%~±3.5%, which showed better results than ±5%(CRYOTOP/China), which is the highest level currently used in the field. The second measured distance accuracy was measured as below ±4.0%, which was superior to ±5%(CRYOTOP/China), which is the highest level currently used in the field. Third, the nitrogen consumption was confirmed to be less than 0.15 L/min at the maximum, which was superior to the highest level of 6 L/min(POLAR BEAR/USA) currently used in the field. Therefore, it was determined that the performance of the personalized cooling management system applied with the thermal imaging camera proposed in this paper was excellent.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.2
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• pp.203-214
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• 2018

Ballast water treated by sodium dichloroisocyanurate (NaDCC) injection method in ballast water management system (BWMS) contains reactive bromine, chlorine species and disinfection by-products (DBPs). In this study, we conducted whole effluent toxicity (WET) testing and ecological risk assessment (ERA) to investigate its ecotoxicological effects on the marine environment. WET testing was carried out for eight marine and fresh water organisms, i.e. diatom, Skeletonema costatum, Navicula pelliculosa, green algae, Dunaliella tertiolecta, Pseudokirchneriella subcapitata, rotifer, Brachionus plicatilis, Brachionus calyciflorus and fish, Cyprinodon variegatus, Pimephales promelas. The WET test revealed that diatom and green algae were the only organisms that showed apparent toxicity to the effluent; it showed no observed effect concentration (NOEC), lowest observable effect concentration (LOEC) and effect concentration of 50 % (EC50) values of 25.0 %, 50.0 % and over 100.0 %, respectively, in seawater conditions. In contrast, rotifer and fish showed no toxicities to the effluent in the all salinity conditions. Meanwhile, chemical analysis revealed that the BWMS effluent contained total of 25 DBPs such as bromate, isocyanuric acid, formaldehyde, chloropicrin, trihalomethanes (THMs), halogenated acetonitriles (HANs) and halogenated acetic acids (HAAs). Based on ERA, the 25 DBPs were not considered to have persistency, bioaccumulation and toxicity (PBT) properties. The ratio of predicted environmental concentration (PEC) to predicted no effect concentration (PNEC) of the all DBPs did not exceed 1.0 for general harbour environments, but isocyanuric acid, tribromomethane, chloropicrin and monochloroacetic acid exceed 1.0 for near ship environments. However, when NOEC (25.0%) of the WET test results where actual effluent was applied, it was concluded that the NaDCC injection method did not have unacceptable ecological risks to the general harbor including near ship environments.

• Journal of Pharmaceutical Investigation
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• v.40 no.spc
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• pp.33-43
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• 2010

Hyaluronic acid (HA) is a biodegradable, biocompatible, non-toxic, non-immunogenic and non-inflammatory linear polysaccharide, which has been used for various medical applications including arthritis treatment, wound healing, ocular surgery, and tissue augmentation. Because of its mucoadhesive property and safety, HA has received much attention as a tool for drug delivery system development. It has been used as a drug delivery carrier in both nonparenteral and parenteral routes. The nonparenteral application includes the ocular and nasal delivery systems. On the other hand, its use in parenteral systems has been considered important as in the case of sustained release formulation of protein drugs through subcutaneous injection. Particles and hydrogels by various methods using HA and HA derivatives as well as by conjugation with other polymer have been the focus of many studies. Furthermore, the affinity of HA to the CD44 receptor which is overexpressed in various tumor cells makes HA an important means of cancer targeted drug delivery. Current trends and development of HA as a tool for drug delivery will be outlined in this review.

• 한국신재생에너지학회:학술대회논문집
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• 2011.05a
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• pp.220.1-220.1
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• 2011

In recent years, the importance of Carbon Capture and Storage (hereafter CCS) is growing bigger and bigger. The development and commercialization of CCS technology are concerned for reducing carbon dioxide($CO_2$) emissions. For the most studies, the technology of $CO_2$ storage is known as the geological storage, ocean sequestration, mineral carbonation, industrial utilization, and so on. The geological storage is adjudged the most reasonable technology from economic and environmental aspects. Generally, the $CO_2$ geological storage is comprised of compression - transportation - drilling/injection - storage/management process. The critical problem is a leakage of $CO_2$ in all process. For resolving a leakage problem, it is necessary to predict and build a monitoring system. Those systems are proved safety of a leakage and received positive social perceptions of $CO_2$ geological storage. For those reasons, a risk assessment of $CO_2$ geological storage is required. A risk assessment is an estimated process of the possible effects when spilling $CO_2$. Although numerous studies of risk assessment have studied, it is incomplete to evaluate a risk and disaster quantitatively. The risk assessment will be developed for domestic application and safe $CO_2$ geological storage considering characteristics of Korea.

• 한국가스학회:학술대회논문집
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• 2007.04a
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• pp.180-185
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• 2007

Likelihood analysis was used for the revision of release probability/frequency in chlorine Injection facilities used in chlorine process. Typically these facilities consist of pressure cylinder, vaporizer, pipeline, measuring equipment and safety equipment. This paper described the incident scenarios considered, likelihood analysis procedure and the selection and application of basic events and for failure rates of mechanical components. Human errors were also considered. The major objective of this paper is to estimate the likelihood of each determined incident scenarios. We estimated failure rates of mechanical components based on likelihood analysis procedure. Human errors were also considered. It was estimated to have $5.73{\times}10^{-5}\;Cl_2$ leak per year during the major $Cl_2$ handling process. The probability of failure in scrubber system was $4.11{\times}10^{-2}/demand$.