• KSBB Journal
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• v.21 no.4
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• pp.267-272
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• 2006

Five strains producing ethanol were isolated from soil near traditional alcohol production factory in Gwangju, Korea. One of the isolated strains maintained relatively stable ethanol production in shaking culture. The isolated strain KJ was proved to be Saccharomyces italicus, based on several biochemical and morphological tests containing assimilation of carbon compounds. In investment of the most suitable carbon for ethanol production, ethanol concentration of 5.46 g/L and yield of 0.53 g-ethanol/g-glucose were obtained in condition of glucose 10 g/L in YM medium. Experimental optimal conditions for ethanol fermentation by S. italicus KJ were as follows; temperature $30^{\circ}C$, initial pH 5.0, initial concentration 10% of glucose, anaerobic condition in the liquid cultivation. When enzymatically saccharified food wastes(SFW) were used as the production medium, ethanol production yield was 0.57 g-ethanol/g-reducing sugar. Therefore, SFW will contribute to lower the production cost of ethanol for industrial application.

• Korean Journal of Microbiology
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• v.16 no.1
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• pp.1-10
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• 1978

In order to isolate and identify of the yeasts in Chinese cabbage Kimchi, was propared and fermented by traditional methods. 40 strains of the yeast were isolated from the Kimchi juice. As the results of the serial experiemnts on morphological, cultural and physiological properties, according to the methods of Lodder, Irzuka, Barnett and Hasegocwa, 13 species of S genera were identified as the followings ; Brettanomyces claussenii, Candida bogoriensis, C.cacaoi, C.guilliermondii, Citeromyces matritensis, Kluyveromyces vaeronae, Pichia membranaefacience, Rhodtorula glutinis, Saccharomyces bayanus, S, cerevisiae, S. pretoriensis, S. italicus, and Torulopsis salmanticensis.

• KSBB Journal
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• v.21 no.6 s.101
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• pp.474-478
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• 2006

For the economically feasible production of ethanol, utilization of SFW (saccharified food wastes) as substrate for synchronous saccharification and fermentation (SSF) process was developed in this study. When 200 g of food wastes and 40 mL of enzyme ($amylase activity,\;3.0\;U/m{\ell}$) were reacted, production rate of reducing sugar was $5.84\;g/{\ell}{\cdot}h$, and consumption rate was $-3.88\;g/{\ell}{\cdot}h\;at\;35^{\circ}C$ So suitable condition of SSF was concluded at temperature of $35^{\circ}C$. Also, optimal enzyme concentration of SSF was concluded in $2.0\;U/m{\ell}$, at this condition, the production rate of reducing sugar was $4.80\;g/{\ell}{\cdot}h$ At SSF process, when 50 g of food wastes was supplied in 12 h interval, $64\;g/{\ell}$ of ethanol and 0.45 g-ethanol/g-reducing sugar in yield were obtained in 120 h fermentation. Thus, the technology of high yield of ethanol production using food wastes was confirmed. And semi-continuos SSF system for cutting off cost of enzymatic saccharification was developed in this study.