• Korean Journal of Microbiology
• /
• v.13 no.3
• /
• pp.109-115
• /
• 1975

DNA's were extracted from Saccharomyces cerevisiae and Mycobacterium phlei and the damaging cell walls of these microoragnisms were examined under an electron microscope in the extraction process in which a number of physico-chemical tratments of cells was involved. While the DNA was easily extracted from S. cerevisiae using conventional meylelded very little DNA, of M. phlei was extremely difficult to isolate and yielded very little DNA, applying various methods of isolation published earlier. When the cell walls of S. cerevisiae were examined with the electron microscope, they were not yet damaged even after the cells were treated with sodium lauryl sulfate(SLS) and ethylene diamine tetracetic acid(EDTA), but they were completely destroyed by the treatment of sodium perchlorate followed by the addition of chloroform and a vigorous agitation. Oozing cytoplasm through the broken cell walls was also observed. In the extraction of DNA from M.phlei, the pronase was not effective at the aerobic environment of the sample. When phenol was applied at the last step of DNA isolation, an extreme damage mass yielding little DNA into the solution. Unlike the cells of S.cerevisiae.M.phlei cells showed a tendency of aggregation, thus the destruction of cell walls by sodium hydroxide was seen only on the walls of peripheral cells in the aggregated mass, leaving the walls of the inner cells undamaged.

• The Korean Journal of Mycology
• /
• v.15 no.4
• /
• pp.260-267
• /
• 1987

The effect of Ganoderma lucidum extract on Saccharomyces cerevisiae growth and physiology has been investigated. S. cerevisiae was inoculated in Henneberg solution medium into which 0, 0.1, 0.5 or 1.0% extracts of G. lucidum were added respectively and it was fermented at $30^{\circ}C$ for 5 days, respectively. Cell number of S. cerevisiae has increased according to the concentration as in order of distilled water(Dw) extracts 1.0% added>ethanol(Et) extracts 1.0% added>Dw extracts 0.5% added>Et extracts 0.5% added>Dw extracts 0.1% added>Et extracts 0.1% added group compared to control group(extracts 0% added) and in Dw extracts 1.0% added group the number has increased than those of control group after the fermentation of 72 hours. Weights of dried yeast cell have increased in each treated group than those of control group and it increased about 1.7 times in each Dw 1.0%, Et 1.0% group than those of control group after fermentation of 120 hours. The more the extracts of G. lucidum was added, the more alcohol levels increased during fermentation. The rate of carbon dioxide production per G. lucidum extract medium was faster than those of control group as G. lucidum extract was increasingly added.