• Title/Summary/Keyword: Saccharomyces cerevisiae JUL3

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Optimization of Medium Components for Cell Mass Production of Saccharomyces cerevisiae JUL3 using Response Surface Methodology (반응표면분석법을 이용하여 Sacharomyces cerevisaeJUL3의 균체량 생산을 위한 배지조성 최적화)

  • Kim, Young-Hwan;Kang, Seong-Woo;Lee, Jong-Ho;Chang, Hyo-Ihl;Yun, Cheol-Won;Paik, Hyun-Dong;Kang, Chang-Won;Kim, Seung-Wook
    • KSBB Journal
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    • v.21 no.6 s.101
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    • pp.479-483
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    • 2006
  • [ ${\beta}-Glucan$ ], one of the cell wall components, is most plentiful polysaccharides in cell wall and has several advantages in immune system. In yeast ${\beta}-glucan$ is mainly contained in the yeast cell wall, and thus it is important to produce high levels of cell mass for the mass production of yeast ${\beta}-glucan$. The best carbon and nitrogen sources on cell mass production were high fructose syrup and yeast extract. Response surface methodology (RSM) was very potential tool for the optimization of process factor and medium component. It was applied to estimate the effects of medium components on the production of cell mass. Optimal concentrations of high fructose syrup and yeast extract by response surface methodology were 8.0% (v/v) and 5.2% (w/v), respectively and the cell mass predicted was $17.0\;g/{\ell}$ at 20 h of cultivation.