• 한국미생물·생명공학회지
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• 제11권3호
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• pp.181-185
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• 1983

증자하지 않은 Cassava의 생전분의 ethanol 발효생산을 위하여 Aspergilius shirousami 가생산하는 당화효소를 이용하여 단순당화 및 동시당화 발효를 하였다. Cassava 생전분의 당화률은 증자한 것에 비해 30% 정도였으며 10배의 효소를 가하여도 60%밖에 안되었으나. 동시당화-발효에서는 당화율이 크게 증가하여 ethanol 생성량이 증자한 전분에 비해 90% 이상이 되었다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2009년도 춘계학술대회 논문집
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• pp.418-421
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• 2009

Effects of aqueous ammonia soaking to three annual plants (hemp woody core, tobacco stalk and corn stover) awere investigated to focus on the enzymatic saccharification characteristics change by this treatment. At two different levels of treatment ($90^{\circ}C$-16 h and $45^{\circ}C$-6 days), higher temperature treatment led to more enzymatic saccharification of cellulose to glucose by commercial cellulase mixtures (Celluclast 1.5L and Novozym 342 from Novozyme Korea). Difference among annual plants were significant. corn stover was the best response to enzymatic saccharification of cellulose and xylan by comercial enzymes all treatment conditions but tobacco stalk was the worst response to all of them. chemical composition or physical structure difference may brought this difference.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.297-302
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• 1999

Simultaneous saccharification and fermentation (SSF) experiments were carried out with a lignocellulosic biomass. The effects of temperature on enzymatic saccharification and the ethanol fermentation were also investigated. The batch SSF process gave a final ethanol concentration of 10.44 g/l and equivalent glucose yield of 0.55 g/g, which was increased by 67％ or higher over the saccharification at 42℃. The optimal operating condition was found to vary in several parameters, such as the transmembrane pressure, permeation rate, and separation coefficient, related to the SSF combined with membrane system (semi-batch system). When the fermentation was operated in a semi-batch mode, the efficiency of the enzymes and yeast lasted three times longer than in a batch mode.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1355-1361
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• 2006

The simultaneous saccharification and fermentation (SSF) process consists of concurrent enzymatic saccharification and fermentation. In the present cybernetic model, the saccharification process, which is based on the modified Michaelis-Menten kinetics and enzyme inhibition kinetics, was combined with the fermentation process, which is based on the Monod equation. The cybernetic modeling approach postulates that cells adapt to utilize the limited resources available to them in an optimal way. The cybernetic modeling was suitable for describing sequential growth on multiple substrates by Brettanomyces custersii, which is a glucose- and cellobiose-fermenting yeast. The proposed model was able to elucidate the SSF process in a systematic manner, and the performance was verified by previously published data.

• 동아시아식생활학회지
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• 제11권1호
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• pp.11-18
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• 2001

The saccharification and sensory characteristics of Sikhe, Korean traditional beverage of saccharified rice, made from thirteen glutinous rice varieties and one of non-glutinous rice named I1pum were examined. During saccharification, Shinsunchalbyeo, Whasunchalbyeo, Yukdononhrimna 1 and Hangangchalbyeo showed the highest sweetness determined by refractometer. Yukdononhrimna 1 was good for the morphology and texture of cooked rice kernel during saccharification. There was interrelationship between contents of released reducing sugar and sweetness determined by refractometer during preparation of Sikhe. The overall acceptability determined by sensory evaluation of Sikhe made from Yukdonongrimna 1, was slightly higher than that of Sikhe made from Ilpum, the non-glutionous rice variety. Yukdononhrimna 1 was considered to the most suitable varieties of Sikhe.

• 한국자원식물학회지
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• 제21권3호
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• pp.222-225
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• 2008

Chemical composition and enzymatic saccharification characteristics of hemp woody core were investigated by their chemical composition analysis and enzymatic saccharification with commercially available cellulases (Celluclast 1.5L and Novozym 342). Hemp woody core have higher xylan and lower lignin contents than its bast fiber. Based on hemicelluloses and lignin composition, hemp woody core is similar with hardwood biomass. However, cellulose was more easily converted to glucose than xylan to xylose and this trend was confirmed both hemp woody core and yellow poplar. Hemp woody core biomass shows higher saccharification than yellow poplar (hardwood biomass) based on cellulose and xylan hydrolysis. With easier enzymatic saccharification in cellulose and xylan, and similar chemical composition, hemp woody core have better biorefinery feedstock characteristics than hardwood biomass.

• 한국식생활문화학회지
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• 제15권1호
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• pp.1-8
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• 2000

This study was to investigate the effects of concentration of malt extract in Sikhye manufacture on saccharification time, shapes of saccharified rice(the cooked rice) and sensory evaluation score of Sikhye. The optimum concentration of malt extract to reduce saccharification time and to keep desirable shapes of saccharified rice was 4 times (rice 24g, malt 28g and D.W. 240ml) as suitable as base composition formula(rice 6g, malt 7g and D.W. 240ml). The shapes of saccharified rice were influenced by the concentration of malt extract, the saccharification time and the shapes of steamed rice before saccharification. A good taste(softness) of Sikhye rice and the desirable shapes of saccharified rice were more suitable in the case of a small amount of unsaccharified starch than in the case of finishing saccharification. The optimum saccharification time to keep the desirable shapes of saccharified rice was 240min and also was desirable between 210 and 270min. To keep a good taste(softness) and the desirable shapes of saccharified rice, and to reduce the manufacturing time, it is desirable to in mass production of Sikhye add 3 times more water after making Sikhye in the ratio of rice 24g, malt 28g and water 240ml. In this case the whole amount will become 4 times as much as the original one.

• 펄프종이기술
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• 제41권5호
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• pp.15-19
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• 2009

Ammonia soaking treatment was introduced for hemp woody core pretreatment to increase enzymatic saccharification of polysaccharides. Portions of the xylan, cellulose, and lignin were removed by aqueous ammonia soaking, which improved the enzymatic saccharification of cellulose and xylan. Following ammonia soaking, 37% ($50^{\circ}C$-6 day treatment) to 61% ($90^{\circ}C$-16 h treatment) of the cellulose was converted to glucose and 33% ($50^{\circ}C$-6 day treatment) to 48% ($90^{\circ}C$-16 h treatment) of the xylan to xylose. Cellulose responded better to enzymatic saccharification than did xylan after the ammonia soaking treatment. Aqueous ammonia soaking pretreatment was more effective than electron beam irradiation for increasing enzymatic saccharification of xylan and cellulose in hemp woody core.

• 한국미생물·생명공학회지
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• 제19권5호
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• pp.497-503
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• 1991

Packed bed column과 continuous stirred tank reactor에서 고정화된 glucoamylase의 catalytic activity를 비교하였다. Settling chamber를 이용한 연속애화, 당화 공정을 사용하여 액화된 전분으로부터 포도당을 연속적으로 생산하였다. . Chitin에 고정화된 glucoamylase에 의한 당화실험에 있어서는 dextrin의 농도가 100g/l일 때, 체류시간 20분 동안 20의 당화수율을 나타냈다.

• 한국식품저장유통학회지
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• 제24권2호
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• pp.196-205
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• 2017

전통 음료인 식혜의 기능성 강화를 위한 연구의 일환으로 섬애약쑥 추출물을 0. 5, 10, 15 및 20% 첨가하여 식혜를 제조하고, 5시간 동안 당화 시키면서 1시간 간격으로 품질특성을 분석하였다. 섬애약쑥 추출물을 첨가한 식혜의 탁도는 당화 2시간에 최고치를 보인 후 점차 감소하는 경향을 나타내었으며, 당화 5시간에는 섬애약쑥 추출물 5-15% 첨가군 간에는 유의적인 차이가 없었고, 20% 첨가군은 유의적으로 높았다. 쑥 식혜의 명도값은 당화 완료 후 모든 실험군에서 유의적인 차이가 없었다. 적색도와 황색도는 당화시간이 경과하고 섬애약쑥 추출물의 첨가량이 많을수록 더 높은 경향이었다. 당화 전 pH는 섬애약쑥 추출물의 첨가 비율에 따른 유의차는 없었고, 당화 완료 후 5.68-5.73의 범위로 증가하였다. 가용성 고형분 및 환원당의 함량은 당화 시간의 경과와 섬애약쑥 추출물의 첨가 비율이 많을수록 높아지는 경향이었다. 총 폴리페놀 화합물의 함량과 DPPH 라디칼 소거활성도 당화 시간과 섬애약쑥 추출물의 첨가와 정의 상관관계를 나타내며 높아졌다. 관능평가 결과에서는 섬애약쑥 추출물 5% 첨가군은 대조군과 차이가 없었으나 20% 첨가군의 경우 강한 색과 쑥 향으로 인해 기호도가 낮았고 전체적인 기호도도 유의적으로 낮았다. 이상의 결과를 종합하여 볼 때 섬애약쑥 추출물을 첨가한 식혜의 경우 쑥 추출액의 첨가량이 높을수록 항산화활성이나 이화학적 특성은 향상되었지만 관능평가에서는 오히려 기호도가 낮아지므로 적정 첨가비율은 15% 이내가 적합한 것으로 판단된다.