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Influence of Cooling Rate, Developmental Stage and Addition of Sugar on Cryopreservation of Pearl Oyster (Pinctada Fucata Martensii) Larvae

  • Park, Youn-Hee;Chang, Young-Jin
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.103-103
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    • 2002
  • This study was conducted to investigate cryopreservation of pearl oyster, Pinctada fucata martensii larvae. Four cooling rates (-0.25, -0.5, -0.75 and -1.0$^{\circ}C$/min.) were used to examine a proper cooling rate during cryopreservation of trochophores before seeding temperature (-12$^{\circ}C$). Seven developmental stages (early and late trochophores, early and late D-shaped larvae and early, middle and late umbo stage larvae) and different sugars (fructose, glucose and sucrose) were used to investigate optimal larval stage and effective sugar in cryopreservation of larvae. The survival rates of frozen-thawed trochophores increased at cooling rate of -1.0$^{\circ}C$/min. As larval developing, survival rate of frozen-thawed larvae increased, except umbo stage larvae, and especially late D-shaped larvae highly survived as 91%. Addition of sugar revealed positive effect on cryopreservation in this experiment and 0.2 M glucose and sucrose mixed with 2.0 M dimethyl sulfoxide significantly enhanced survival rate of larvae (P<0.05). The results of our study indicate that desirable cooling rate, developmental stages of larvae and effective sugar far cryopreservation of pearl oyster, P. fucata martensii larvae are -1$^{\circ}C$/min, late D-shaped larvae and 0.2 M glucose and sucrose, respectively.

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Optimal Mixing Conditions of Smoothie Added Small Black Soybean Using Response Surface Methodology (쥐눈이콩 첨가 스무디의 제조조건 최적화)

  • Joo, Na-Mi;Park, So-Yeon
    • Korean journal of food and cookery science
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    • v.25 no.3
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    • pp.337-344
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    • 2009
  • The principal objective of this study was to determine the optimal mixing conditions of three different amounts of small black soybeans, sugar, and plain yogurt, for the preparation of a small black soybean smoothie. The experimental design utilized herein was based on the Central composite design methodology of response surface, which included 16 experimental points, including 2 replicates for the small black soybeans, sugar, and plain yogurt. The physiochemical and sensory properties of the test were measured, and these values were applied to the mathematical models. A canonical form and perturbation plot showed the influence of each ingredient on the mixed final product. The L value and a value decreased with increasing quantities of small black soybeans, but the b values increased with greater additions of small black soybeans. Sweetness increased with increasing amount of added sugar. The results of sensory evaluation showed very significant values for color (p<0.01), taste (p<0.05) and overall quality (p<0.05) in the predicted model. As a result, the optimum formulations by numerical and graphical methods were calculated as follows: small black soybean 79.46 g, plain yogurt 275.07 g, sugar 21.20 g.

Process Development of Alcohol Production by Extractive Fermentation(III) -An Optimum Composition of PEG/Dx for Extractive Alcohol Fermentation- (추출발효에 의한 알코올.제조공정 개발(III) -추출 알코올 발효에 최적인 PEG/Dx의 조성-)

  • 허병기;김진한목영일
    • KSBB Journal
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    • v.8 no.2
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    • pp.178-184
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    • 1993
  • Extractive fermentations with the extract of Jerusalem artichoke in an aqueous-two-phase-system of polyethyleneglycol(PEG) and dextran(Dx) were investigated to obtain the effects of composition of PEG and Dx on both fermentation ,characteristics and partition ratio of alcohol. The specific growth rate of K. Fraglis CBS 1555 increased with a decrease of concentration of PEG and Dx. It augmented along with concentration of initial sugar up to 80g/l but decreased thereafter. The specific production rate of alcohol showed a rising tendency up to 100g/lof initial sugar, whereafter represented a decreasing trend. The partition ratio of alcohol between two phases augmented according to decrease of Dx comic. and increase of PEG cone. regardless of initial sugar concentrations. The ratio, however, decreased with Increment of initial sugar concentration at constant composition of PEG and Dx. The partition coefficient of alcohol had an ascending effect to the increase of PEG cone, but it had little effect on the changes of concentrations of Dx and initial sugar. The present study suggests that the optimum composition of PEG and Dx in the aqueous-two-phase-system by extractive fermentation were around 6.5%(w/v) of PEG and 3%(w/v) of Dx in considerations of emulsion state, sedimentation and separation of two phases, alcohol partition ratio, and specific growth rate.

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Changes of Quality in the Osmotic Dehydration of Cherry-Tomatoes and optimization for the Process (방울토마토의 삼투건조시 품질의 변화와 공정의 최적화)

  • 윤경영;윤광섭;이광희;신승렬;김광수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.5
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    • pp.866-871
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    • 1997
  • This study was carried out to determine the effect of osmotic dehydration as pretreatment on the qualities of dried cherry-tomatoes. The weight reduction and solid gain in osmosed cherry-tomato were increased by increasing sugar concentration, immersion temperature and time; among three parameters, the immersion temperature affected more than sugar concentration and immersion time did. The moisture content was decreased as increasing sugar concentration, immersion temperature and time, and it was the lowest at the osmotic conditions of 7$0^{\circ}C$, 60$^{\circ}$Brix and 11hr. To determine the optimum processing condition by RSm, the polynomial optimum models were established. The regression models was significant (p<0.05). It was used contour plots to optimize osmotic dehydration. The optimum condition for osmotic dehydration as pretreatments for drying of cherry-tomatoes were immersion temperature of 47~53$^{\circ}C$, sugar concentration of 39~43$^{\circ}$Brix, and immersion time of 7hr, in which process conditions were 78~86% moisture content, 8.5~10$^{\circ}$Brix sugar content and 80~86% weight reduction.

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Substitution Effect of Sorbitol for Sugar on the Quality Stability of Yu Ja Cheong(Citron product) (솔비톨의 당대체효과에 의한 유자청의 품질안정성에 관한연구)

  • 차용준;이상민;안병주;송능숙;전수진
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.19 no.1
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    • pp.13-20
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    • 1990
  • Yu Ja cheong(Citron product) one of the traditional drinks was processed by a modified method to improve the quality,. Sugar(38%) and sorbitol(12%) were added as partially substi-tuted sugar and experimented about quality stability during storage at 20$\pm$2$^{\circ}C$ comparing with conventional 50% sugar added product. Chemical compositions of the acidity 40. 46mg% total Vitamin and 28.5mg% amino-N and pH was 3.7 During the storage pH reducing sugar and amino-N contents increased slightly while total acididty decreased slightly and reduced vitamin C occupied most part of total vitamin C after proessing was converted to oxidized vitamin C greatly at 70 days of storage. While L a and b values deceased in between modified one and conventional product. e value increased continually during storage {{{{ {C }_{16 { }:_{ } 0 } }}}} {{{{ {C }_{18 { }:_{ }0 } }}}} {{{{ {C}_{18 { }:_{ }1 } }}}} and {{{{ {C }_{18 { }:_{ }2 } }}}} contnts in fatty acid and citric acid itaconic acid malic acid and succinic acid in organic acid were the major componets in both products. Judging from the results of experiments during storage the quality of the modified product was compared quite well with that of vonventional one during storage.

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Glycemic Control Effects of Sanyak (Dioscoreae rhizoma) extract in Prediabetic Stage Patients (산약 추출물이 당뇨병 전단계 피험자의 혈당조절에 미치는 영향)

  • Cho, Suck-In;Son, Mi-Won;Hong, Kwon-Eui
    • The Journal of Korean Medicine
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    • v.31 no.5
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    • pp.146-166
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    • 2010
  • Objective: Few data were available on glycemic control at the early prediabetic stage, though it is the important stage to prevent diabetes mellitus. The purpose of this research was to examine the glycemic control effects of herb medicine treatment (DA-9802) in prediabetic stage patients Methods: In this randomized, single blind, placebo-controlled study, we compared Sanyak extract (DA-9802) with placebo for the treatment of glycemic control effects in prediabetic stage patients. 45 volunteers who satisfied the requirements were enrolled in the study. They took Sanyak extract (DA-9802) or placebo treatment three times a day for 8 weeks. Blood sugar, Total Symptom Score (TSS) and blood serum were measured at weeks 0, 4, and 8. Results: In the DA-9802 group, fasting blood sugar changes between before 8 weeks significantly decreased. Fasting blood sugar, fasting blood sugar gaps between before and after treatment for 8 weeks, insulin changes and C-peptide significantly decreased at 8 weeks compared with those of the placebo group. Conclusion: Herb medicine treatment (DA-9802) is effective in glycemic control on prediabetic stage.

Study on Tissue Distribution of Recombinant Human Alpha-Interferon (유전자 조작 알파 인터페론의 조직분포에 관한 연구)

  • Kim, Je-Hak;Lee, Hye-Sun;Kim, Dal-Hyun;Cho, Nam-Jin;Kwack, Kyu-Bum
    • Journal of Pharmaceutical Investigation
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    • v.17 no.4
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    • pp.213-216
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    • 1987
  • The distribution features of recombinant human $alpha-interferon(rHuIFN-{\alpha}A)$ and $^{14}C-radiolabeled\;rHuIFN-{\alpha}A\;(^{14}C-rHuIFN-{\alpha}A)$ were investigated in ICR mice after i.v. injection. The level of $rHuIFN-{\alpha}A$ in the kidney was significantly higher than those in lung and liver at 10min after the injection. But the level was reduced significantly at 60min. The level of radioactivity in the kidney was also significantly higher than those in other organs after i.v. injection of $^{14}C-rHuIFN-{\alpha}A$, but it was reduced at much slower speed than was $rHuIFN-{\alpha}A$. These results show that interferon is distributed repidly and the kidney is the main site of distribution and metabolism of $rHuIFN-{\alpha}A$.

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Changes in Oil, Tannin, Total Sugar Contents and Yield after Flowering in Peanut

  • Lee, Sung-Woo;Park, Chang-Hwan;Kang, Chul-Whan;Kim, Sok-Dong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.2
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    • pp.159-162
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    • 1999
  • This study was carried out to determine the optimum harvest date of unshelled immature peanuts and dried kernels from 60 to 120 days after flowering. Fresh pod yield of spanish-type variety, 'Shaedl-tangkong(SD)' reached a peak of 9,140kg/ ha at 70 (days after flowering(Aug. 13) while that of virginia-type variety, 'Daepoongtangkong(DP)' reached a peak of 8,820kg/ha at 90 days(Sept. 11) after flowering. SD and DP showed maximum fresh kernel yield of 6,090 and 6,470kg/ha at 90 days after flowering (Sept. 11), respectively, while dry kernel yield reached a peak of 3,300 and 3,720kg/ha at 110 days(Oct. 1), respectively. Oil content of SD and DP were the highest at 90 days and 100 days after flowering, respectively and the oil content of two varieties increased rapidly from 60 to 90 days. Tannin content of the seed hull of SD increased continuously until 110 days after flowering while that of DP maximized at 100 days. The tannin content of the two varieties increased rapidly from 60 to 100 days. Total sugar of SD and DP showed highest content at 60 days and 70 days after flowering, respectively and suger content decreased very rapidly until 80 days and after that sugar content kept nearly constant. Oil, tannin and total sugar content of spanish-type SD were higher than those of virginia-type DP.

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The Positive Effect of LuoHanGuo as Sugar Substitute on Blood Glucose and Metabolism in Streptozotocin-Induced Diabetic Mice

  • Lee, Yoon Jeong;Jeong, Jain;Kim, Myoung Ok;Nam, Ju-Ock
    • Applied Microscopy
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    • v.46 no.3
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    • pp.140-149
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    • 2016
  • This study intended to compare the effects of the sugar-Omija extract tea (SO) and LuoHanGuo-Omija extract tea (LO) to blood glucose levels and metabolism among streptozotocin (STZ)-induced diabetic mice and to prove the positive effects of LuoHanGuo extract as a sugar substitute. According to the results, the rate of blood glucose increase and the blood glucose level was reduced, and the weight was increased. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride, low density lipoprotein cholesterol, and total cholesterol was decreased, and albumin, total protein, and high density lipoprotein cholesterol (HDL-C) was increased in the STZ/LO group as compared to the STZ group. Compared to the STZ/SO group, the rate of blood glucose increase and glucose tolerance was decreased. AST, ALT, and total cholesterol was decreased, and HDL-C level was high. In conclusion, the LO displayed hypoglycemic effect, prevented weight loss caused by diabetes, recovered liver function, and improved lipid metabolism. In addition, such positive effects were obviously shown during comparison with the experimental group treated with the SO. Therefore, the LO were considered as favorable food supplements that can be safely taken by persons with diabetes, obesity, hypertension, and heart disease; they have to control their sugar intake as well as their diet.

Quality Characteristics of Sorbitol Added Walnut-sulgi

  • Choi, Jung-Hee;Lee, Jung-Hee;Choi, Young-Hee;Lee, Yae-Ja;Lee, Seung-Min
    • Food Quality and Culture
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    • v.3 no.2
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    • pp.59-63
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    • 2009
  • As one of study efforts to develop a food product that can satisfy the taste of modern people and increase the usage of the popular local product of walnut in the region of Chunan, the current study developed a no sugar added functional walnut-sulgi. The walnut-sulgi was manufactured by adding walnut powder into typical sulgi rice cake. While manufacturing the walnut-sulgi rice cake, the health beneficial sugar alcohol ingredient of sorbitol was added instead of commonly used sugar to specialize the walnut-sulgi as a functional rice cake. As the result of such effort, a soft and moist walnut-sulgi was produced. The color of the newly developed walnut-sulgi is white and has a pleasing taste, and its consumer acceptability was higher than the sugar added walnut-sulgi by showing much soft sweetness and textural properties. Considering the facts that sorbitol has a lower glycemic index (GI) than sugar and the content of unsaturated fatty acids that are insufficient in rice cake could be increased, the newly developed sorbitol added walnut-sulgi is thought to be an appropriate functional rice cake that can attractively appeal to obesity and diabetes concerning modern people.