• 대한한방내과학회지
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• 제33권4호
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• pp.367-386
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• 2012

Objectives : The object of this study was to observe the effects of Gamioryung-san (GOS), which consists of 22 types of herbs, on streptozotocin (STZ)-induced diabetic nephropathy rats. Methods : Three different dosages of GOS were orally administered once a day for 28 days from 3 weeks after STZ treatment. Six groups, each of 8 rats per group were used. Changes on the body weights, blood glucose levels, serum BUN and creatinine levels, urine volumes, and UAER were observed with changes on the kidney malondialdehyde contents and glutathione, dismutase and catalase contents. In addition, histopathology of kidney, pancreas, thymus and spleen were observed. The results were compared with antioxidant silymarin 100 mg/kg, of which the effects on STZ -induced diabetes and related complications are already confirmed. Results : As a result of treatment of GOS 800, 400 or 200 mg/kg for 28 days, STZ-induced decreases of body weights, hyperglycemia, atrophic changes of pancreatic islets with decreases of insulin-immunoreactive cells and decreases of glucagon -immunoreactive cells were inhibited dose-dependently. Increases in kidney weight, serum BUN and creatinine levels, urine volumes, UAER, vasodilated atrophic glomerulus and abnormal tubules were inhibited dose-dependently. Also increases of kidney MDA contents and decreases of GSH contents, SOD and CAT activities, decreases of thymus and spleen weights, and atrophic changes at histopathological observation were also inhibited. The effects of GOS 400 mg/kg showed similar effects to silymarin 100 mg/kg. Conclusions : These results suggest that 400 mg/kg of GOS retarded the STZ-induced diabetic nephropathies as similarly to silymarin 100 mg/kg, through modulations of oxidative stress and immune systems.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.261.2-261.2
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• 2002

Hypoglycemic effect of GRA was examined in multiple low dose(MLD) streptozotocin(STZ)-induced diabetic rats with regard to time of administration. Experimental methods: 20 mg/kg of STZ in 100 mM citrate buffer(pH 4.5) was injected intraperitoneally for 5 consecutive days. In co-treatment groups, GRA was administered intraperitoneally for 3 weeks at dose of 150 or 300 kg/kg. (omitted)

• 생약학회지
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• 제37권2호통권145호
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• pp.103-109
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• 2006

Advanced glycation end products (AGEs) formation plays an important role in the progression of diabetic complications. To develop effective herbal formulations with suppression of diabetic nephropathy, a common complication in diabetic patients, we evaluated inhibitory activities of KIOM-79, a new herbal prescription, on the formation of AGEs using in vitro and in vivo model systems. Effects of KIOM-79 on the expression of AGEs, RAGEs (receptor for Advanced glycation end products), type IV collagen and renal $TGF-{\beta}1$ mRNA was also examined in streptozotocon (STZ)-induced diabetic rats. STZ-induced diabetic rats were treated orally with KIOM-79 (250 and $500\;kg^{-1}$ once a day for 13 weeks). In vitro system KIOM-79 suppressed the formation of AGEs $(18.12\;{\mu}g/ml)$. In STZ-induced diabetic rats showing accumulation of AGE and RAGE, pathological examination revealed that KIOM-79 prevented AGE and RAGE deposition in the kidney. In STZ induced diabetic rats, the expansion of mesangial matrix and the glomerular tufts seemed to be larger than those in normal rats. Howεver, after administration with KIOM-79, mesangial metrix and glomerular volume were decreased, and overexpression of type IV collagen was also decreased. Overexpression of renal $TGF-{\beta}1$ mRNA was inhibited significantly. These results suggest that the KIOM-79 might be an effective herbal prescription to prevent or alleviate the progression of diabetic nephropathy.

• 한국식품과학회지
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• 제44권3호
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• pp.367-372
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• 2012

5종의 약용식물을 첨가한 발효홍삼(FRGM) 추출물의 항산화활성 및 ${\alpha}$-glucosidase 저해 활성을 in vitro에서 검토하였고, 또한 STZ로 당뇨를 유발시킨 흰쥐에서 FRGM 추출물의 효능을 살펴보았다. 그 결과, FRGM 추출물은 총 폴리페놀 및 총 플라보노이드 함량이 각각 $22.41{\pm}3.51{\mu}g/mg$, $16.80{\pm}4.22{\mu}g/mg$으로 존재하였고, DPPH 소거 활성을 가지고($RC_{50}=95.57{\pm}7.40{\mu}g/mL$), 또한 높은 ${\alpha}$-glucosidase 저해 활성을 나타내었다. STZ 투여에 의해 증가된 혈청 내 ALT와 AST의 활성이 FRGM 추출물의 투여로 감소하였으며, 간 조직중의 글루타치온 함량은 STZ 투여군에서는 감소되었다가 FRGM 투여시 유의적인 증가를 보였고, 지질과산화물 함량은 STZ 투여시 증가되었다가 FRGM 추출물의 투여시 유의적으로 감소되었다. 또한 간 조직중의 SOD, CAT, GP 그리고 GR의 활성도 STZ 투여로 유의적으로 감소되었다가, FRGM 추출물의 투여로 이들 항산화효소 활성이 유의적으로 증가하였다.

• 생명과학회지
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• 제15권4호
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• pp.619-625
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• 2005

함초 추출물 분말을 첨가하여 유산 발효시킨 함초 요구르트를 streptozotocin-유발 당뇨쥐에 투여하여 항당뇨효과 및 콜레스테롤 저하 효과를 규명하기 위하여 3주간 실험하였다. 당뇨 대조군에 비해 함초 요구르트 투여군에서 혈당치 농도 및 식이 섭취량이 현저히 감소하였다. 경구당부하 실험에서도 당뇨 대조군에 비해 함초 요구르트 투여군에서 현저한 혈당상승 억제작용을 나타내었다. 당뇨쥐에 함초 요구르트와 일반 요구르트 섭취에 의해 혈중 총 콜레스테롤 농도 감소와 더불어 동맥경화지수도 감소하였다. 따라서, 함초 요구르트에 의한 혈당치 및 콜레스테롤 농도 감소효과는 고혈당증 및 고 콜레스테롤 혈중에도 유익한 작용을 할 수 있는 기능성식품 개발 가능성이 있음을 시사하였다.

• 한국식품과학회지
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• 제36권3호
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• pp.465-471
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• 2004

본 실험은 당뇨병 치료에 대한 연구로 우리나라에서 민간요법에 이용되어 오던 식물인 택사에서 혈당강하에 영향을 미친 butanol분획물과 항산화영양소인 비타민 E를 21일간 경구투여 하여 글리코겐, 지질함량 및 지질과산화에 미치는 영향을 검토하였다. 간, 신장 및 폐의 상대적 무게는 당뇨대조군이 정상군에 비해 유의적으로 높은 경향을 보였고 특히 신장은 현저한 비대현상을 나타냈다. 간장 글리코겐 함량은 당뇨대조군에 비해 Ac-VE투여군과 VE투여군에서, 근육의 글리코겐 함량은 VE투여군에서 유의적으로 증가하였다. 간장 콜레스테롤 함량은 당뇨대조군에 비해 Ac투여군과 VE투여군에서 감소하였다. 간장 중성지방 함량은 정상군에 비해 당뇨대조군에서 유의적으로 감소하였으며 당뇨대조군에 비해 모든 당뇨실험군에서 증가하였으며 VE투여군에서 유의적으로 증가하였다. 간장의 MDA 함량은 당뇨대조군에 비해 당뇨실험관에서 감소하였으며 Ac투여군과 Ac-VE투여군에서는 유의적으로 감소하였다. 폐의 경우는 당뇨대조군에 비해 모든 당뇨실험군에서 감소하였으나 유의적인 차이는 없었다. 이상의 결과 streptozotocin으로 당뇨를 유발시킨 흰쥐에서 택사의 투여가 간의 산화적 손상을 감소시키므로써 산화적 스트레스 억제효과를 관찰할 수 있었으며, 비타민 E의 투여가 간장 및 근육의 글리코겐 함량을 증가시키고 중성지방함량을 증가시켰다. 택사와 VE의 상관관계는 보이지 않았고 각자 독자적인 기전에 의해서 발생하는 것으로 사료된다. 그리므로 앞으로 각 조직의 지방대사 및 약물대사효소활성에 비치는 영향에 대해 더욱 연구되어져야 할 것이다.

• Journal of Nutrition and Health
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• 제38권10호
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• pp.801-806
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• 2005

본 연구는 streptozotocin으로 유발된 당뇨 흰쥐에게 동과에서 추출한 각 용매분획물을 14일간 경구투여한 후 혈당과 지질함량을 분석하였으며 다음과 같은 결론을 얻었다. 당뇨대조군과 비교시 실험 14일 후 당뇨실험군에서 체중감소가 낮아졌으며, 실험 10일째부터는 당뇨실험군에서 혈당 수준이 당뇨대조군보다 감소하는 경향을 보이었다. 특히 $CHCl_3$분획물 투여군과 $H_2O$분획물 투여군은 14일째에 유의적으로 낮은 수준을 나타내었다. 당뇨실험군은 혈장 유리지방산 수준에서도 당뇨대조군에 비해 낮은 수준이었으며 $CHCl_{3}$분획물 투여군은 혈장 중성지방 수준에서도 낮게 나타났으나 유의적인 차이는 보이지 않았다. ALT와 AST 활성도는 당뇨대조군에 비해 $H_2O$분획물 투여군에서 유의적으로 낮게 나타났다. 결론적으로 당뇨 유발 흰쥐에게 $CHCl_3$분획물의 투여가 실험적 당뇨에 대하여 체중의 감소를 둔화시키며 특히 혈장 중 포도당의 함량을 유의성있게 감소시키는 것으로 보아 당뇨병에 대한 개선효과가 있다고 사료된다.

• The Korean Journal of Pain
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• 제34권2호
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• pp.176-184
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• 2021

Background: Diabetes-related neuropathic pain frequently occurs, and the underpinning mechanism remains elusive. The periaqueductal gray (PAG) exhibits descending inhibitory effects on central pain transmission. The current work aimed to examine whether inflammatory cytokines regulate mechanical allodynia and thermal hyperalgesia induced by diabetes through the phosphoinositide 3-kinase (PI3K)-mammalian target of rapamycin (mTOR) pathway in the PAG. Methods: Streptozotocin (STZ) was administered intraperitoneally to mimic allodynia and hyperalgesia evoked by diabetes in rats. Behavioral assays were carried out for determining mechanical pain and thermal hypersensitivity. Immunoblot and ELISA were performed to examine PAG protein amounts of interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), as well as their corresponding receptors in STZ rats, and the expression of PI3K/protein kinase B (Akt)/mTOR signaling effectors. Results: Increased PAG p-PI3K/p-Akt/p-mTOR protein amounts were observed in STZ-induced animals, a PI3K-mTOR pathway inhibition in the PAG attenuated neuropathic pain responses. Moreover, the PAG concentrations of IL-1β, IL-6, and TNF-α and their receptors (namely, IL-1R, IL-6R, and tumor necrosis factor receptor [TNFR] subtype TNFR1, respectively) were increased in the STZ rats. Additionally, inhibiting IL-1R, IL-6R, and TNFR1 ameliorated mechanical allodynia and thermal hyperalgesia in STZ rats, alongside the downregulation of PI3K-mTOR signaling. Conclusions: Overall, the current study suggests that upregulated proinflammatory cytokines and their receptors in the PAG activate PI3K-mTOR signaling, thereby producing a de-inhibition effect on descending pathways in modulating pain transmission, and eventually contributing to neuropathic pain.

• Food Science and Biotechnology
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• 제17권2호
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• pp.287-294
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• 2008

Protective effects of cheonggukjang fermented with 20% red ginseng (RC) were observed in streptozotocin (STZ)-induced diabetic rats by measuring levels of blood glucose, serum lipid profiles, and hepatic reactive oxygen species generating and scavenging enzymc activities. RC diet was prepared by mixing with AIN-76 diet at the final concentration 2%, and it was fed to STZ-induced diabetic rats for 3 weeks. The RC dict was significantly improved body weight, feed efficiency ratio, levels of serum glucose, and serum and hepatic lipids in diabetes. The significantly elevated O type activity of xanthine oxidase in diabetes was also greatly decreased by the RC diet. The treatment of RC showed the improved hepatic glutathione s-transferase activities in the diabetic animals. The present study indicates that cheonggukjang fermented with red ginseng could ameliorate STZ-induccd diabetic symptoms such as aggravated blood glucose levels, serum lipid profiles, and even the conditions of oxidative stress.

• 응용통계연구
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• 제22권1호
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• pp.107-114
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• 2009

The present study was carried out to summarize the effect of ginseng in the experimental diabetic rats by meta-analysis related studies. The association measure to test effect of ginseng was the mean difference(MD) between group of rats induced streptozotocin(STZ) and group of rats induced STZ treated with ginseng about the considered effect factors. The level of FI, glucose and TG were significantly reduced(< 0.01), and the level of glycogen was significantly increased by treatment with ginseng (< 0.01) After checking the indication of publication bias for the combined MDs by using the funnel plots, the anti-diabetic effects of ginseng is clearly presented in FI, glucose, TG and glycogen (< 0.05).