• 한국철도학회:학술대회논문집
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• 한국철도학회 2008년도 춘계학술대회 논문집
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• pp.952-963
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• 2008

The collection of KTX trainset fault code is made by DEP software. We can improve software function and update data after understanding DEP software function and software structure. For the improvement of trainset function and its performance, the same improvement of performance and structure change as DEP software, which collects and analyzes train's fault information, are required. Accordingly, we made a research for functional strucfure of KTX fault code collection software, STR3F, variable declaration through Script.rxr file and code analysis method and proceeded research for realizing more improved user environment of DEL software and fault analysis method.

• Journal of Genetic Medicine
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• 제3권1호
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• pp.21-24
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• 1999

To study the X chromesome mosaicism in the cytogenetically pure 45,X Turner syndrome patients, we applied PCR technique using DNAs extracted from archived cytogenetic slides. We amplified the DNAs using nested primers targeted to a highly polymorphic short tandem repeat(STR) of the human androgen receptor gene(HUMARA) for the detection of X chromosome mosaicism. This assay is a very sensitive and useful method which can be applied to the DNAs extracted from archived cytogenetic slides to detect X mosaicism. We have tested 50 normal Korean females to determine whether the HUMARA locus is highly polymorphic among Koreans. 85% of Korean population showed heterozygosity in the HUMARA locus. We analysed the 24 DNAs extracted from archived slides of patients and abortuses with Turner syndrome in cytogenetic analysis. We observed the heterozygosities of 50% from pure 45,X patients, 83% from the patients with mosaic Turner syndrome and 8.3% from the abortuses of pure 45,X. Using the PCR technique of the HUMARA locus in the archived cytogenetic slides, we detected X chromosome mosaicism which could not be detected in cytogenetic analysis.

• BMB Reports
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• 제29권3호
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• pp.183-191
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• 1996

DNA fragments, homologous to the dTDP-D-glucose 4,6-dehydratase gene, obtained from the genomic DNA of Streptomyces antibioticus $T\ddot{u}99$, a producer of the unusual macrolide antibiotic chlorothricin, were cloned and sequenced. This dehydratase gene was designated as oxil. The coding region of the oxil gene is composed of 987 bp, and analysis of the DNA sequence data reveals sequences for the gene products of 329 amino acids (molecular weight of 36,037). The deduced amino acids are 59% identical to the StrE, dTDP-D-glucose 4,6-dehydratase from the streptomycin pathway. The oxil's function was examined by expressing it in E. coli using the T7 RNA polymerase/promoter system (pRSET) to produce an active fusion protein including a his tag. This enzyme shows specificity of substrate, specific only to dTDP-D-glucose.

• Mycobiology
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• 제46권3호
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• pp.192-204
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• 2018

The name Golovinomyces cynoglossi s. lat. is traditionally applied to a complex of morphologically similar powdery mildews on hosts of the plant family Boraginaceae. The current species-level taxonomy within this complex is ambiguous due to the lack of phylogenetic examinations. The present study applied phylogenetic methods to clarify the taxonomy of G. cynoglossi s. lat. Phylogenetic analysis of rDNA ITS sequences retrieved from Asian, European and North American specimens revealed that G. cynoglossi s. lat. collections from different hosts involved several species in five clearly separated lineages. Clade I consists primarily of Golovinomyces cynoglossi s. str. on Cynoglossum. Clade III consists of Golovinomyces sequences retrieved from the host genera Symphytum and Pulmonaria. The taxa within clade III are now assigned to G. asperifoliorum comb. nov. Clade V encompasses G. cynoglossi s. lat. on the host genera Bothriospermum, Buglossoides, Echium, Myosotis, and Trigonotis. The taxa within clade V are now assigned to G. asperifolii comb. nov. The species concerned in this study were lecto- and epitypified to stabilize their nomenclature.

• 분석과학
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• 제24권1호
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• pp.51-59
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• 2011

유전자 감식은 다양한 범죄현장에서 발견되는 생체시료의 분석을 통해 신원을 식별하는 중요한 법과학적 수사과정으로 자리 잡았다. 최근에는 범인이 사용했던 펜, 뺑소니 차량에서의 핸들, 기어, 각종 버튼스위치 등에 남겨져 있는 touch evidence-type sample로 알려져 있는 low copy number (LCN) DNA에서의 A-STR분석을 위해 의뢰되는 감정물들이 증가하는 추세에 있다. 본 연구에서는 총기의 뭉개진 지문 등에 남겨져 있는 touch evidence-type의 LCN DNA를 추출하고 유전자형의 분석 성공률을 확인하고 자 하였다. 4종류의 총기(M16, K1A, COLT 45 권총, M29 리볼버)를 각각 격발한 후 총기별로 4곳의 부위에서 시료를 채취한 다음 LCN DNA의 추출을 위해 Microkit과 $Prepfiler^{TM}$ 등 2종류의 시약을 이용하여 DNA 검출량과 유전자형 분석 성공률을 비교 분석하였다. 분석결과 $Prepfiler^{TM}$가 Microkit에 비해 평균 1.7배 DNA검출량이 많았으며, 유전자형 분석 성공률에 있어서도 Microkit은 0%인데 비해 $Prepfiler^{TM}$에서는 평균 24.9%의 성공률을 보였으며, K1A의 손잡이 부위에서 50.6%의 성공률을 나타냈다.

• 한국식품과학회지
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• 제21권6호
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• pp.795-799
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• 1989

효모 Kluyveromyces fragilis SKD-7001로부터 정제한 ${\beta}-galactosidase$를 이용하여 시유의 유당을 가수분해 시키고, 이 효소의 처리유로 요구르트를 제조하여 이들의 이화학적 성분의 변화를 검토하였다. 효소의 농도를 3.0units/ml 처리시 120분간 $40^{\circ}C$에서 반응시켰을 때 유당의 가수분해율이 75.0%를 나타내었고, 6.0units/ml 처리시 120분간 반응시켰을 때에는 94.6%이었다. ${\beta}-galactosidase$의 처리유로 요구르트를 제조하여 pH, 산도, 유산균의 생균수를 정토해 본 결과, $40^{\circ}C$에서 8시간 매양하였을 때 pH4.1, 산도 1.04% 그리고 유산균의 생균수는 각각 Str. thermophilus가 $6.5{\times}10^8/ml$, L. bulgaricus가 $8.9{\times}10^8/ml$를 나타내었다. 총 아미노산을 분석해 본 결과 총 함량은 대조 요구르트가 2.63%, LH 요구르트가 2.19%로써, LH 요구르트보다 대조 요구르트 함량이 더 높은 것으로 나타났다. 유리 아미노산을 분석해 본 결과 총 함량은 시유의 경우 대조 요구르트가 26.95mg%, LH 요구르트가 17.55mg%로써, LH 요구르트보다 대조 요구르트의 함량이 더 높은 것으로 나타났다. 지방산에 있어서는 LH 요구르트가 대조 요구르트보다 caprylic, capric, lauric acid 등의 저급 지방산 함량이 약간 높은 경향을 보였고, 고급 지방산 중에서는 palmitic acid의 함량이 가장 많은 것으로 나타났다.

• KIEAE Journal
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• 제6권2호
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• pp.11-18
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• 2006

In this research, Conducting an engineering properties experiment, this study examined basic properties of regional Hwangto. The results of experiments are as followings. 1) This study confirmed that a result of examining lime order for Hwangto and comparison of stimulants, this study confirmed that 28 day's strength promotion is found in case of calcium hydroxide(Ca(OH)2) and calcium chloride(CaCl2) stimulant. Finally, it is known the fact that lime highly improves the weak strength of Hwangtoh. 2) As XRD analysis for proving the strength manifestation principle of Hwangto by regions, CSH figure and CASH figure appeared in each regional Hwangto in all the strength areas. This result could be appeared through hydraulicity from reaction of alkali stimulant and water, and pozzolan reaction(CSH figure) and $Str{\ddot{a}}tlingite$ reaction(CASH figure) by silica (SiO2) ingredient and calcium hydroxide (Ca(OH)2) among ingredients of clay, and alumina(Al2O3). 3) In result of strength analysis, It is knowned that the Gyeongsangdo Hwangto is stronger than the Jeollado Hwangto in reactivity.

• Structural Engineering and Mechanics
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• 제8권2호
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• pp.207-231
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• 1999

A versatile 4-node shell element which is useful for the analysis of arbitrary shell structures is presented. The element is developed by flat shell approach, i.e., by combining a membrane element with a Mindlin plate element. The proposed element has six degrees of freedom per node and permits an easy connection to other types of finite elements. In the plate bending part, an improved Mindlin plate has been established by the combined use of the addition of non-conforming displacement modes (N) and the substitute shear strain fields (S). In the membrane part, the nonconforming displacement modes are also added to the displacement fields to improve the behavior of membrane element with drilling degrees of freedom and the modified numerical integration (M) is used to overcome the membrane locking problem. Thus the element is designated as NMS-4F. The rigid link correction technique is adopted to consider the effect of out-of-plane warping. The shell element proposed herein passes the patch tests, does not show any spurious mechanism and does not produce shear and membrane locking phenomena. It is shown that the element produces reliable solutions even for the distorted meshes through the analysis of benchmark problems.

• 한국미생물·생명공학회지
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• 제13권4호
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• pp.417-422
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• 1985

Streptococcus faecalis var. liquefaciens에서 plasmid DNA를 분석한 결과, 4개의 plasmid를 가지고 있었으며, 각각의 대략적인 분자량은 6.8M-dal, 5.2Mdal, 2.6Mdal, 2.1Mdal로 측정되었다. 이 균주를 novobiocin으로 처리하여, 각각 다른 2개의 plasmid가 소실된 2개의 변이주를 얻었다. 이들 균주는 당발효성, 온도감수성, gelatin 용해성, 단백질응고성은 wild type과 동일하였으나 이중 한 균주가 lincomycin과 erythromycin에 대한 감수성을 나타내었다. 따라서 본 실험에서 검출한 4개의 plasmid는 당발효성 등의 특성과는 관련이 없는 것으로 추정할 수 있으며, pSK₂와 pSK₄의 두 plasmid 중 하나는 항생물질 저항성에 관련된 것으로 보인다.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.51-59
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• 2004

Phylogenetic relationships were analyzed among bumblebees using a portion of mitochondrial (mt) 16S ribosomal RNA (16S rRNA). Eight species of true bumblebees and one species of cuckoo bumblebee (Bombini, Apidae), collected from Korea were included in the analysis. Also, one species of true bumblebee imported from several foreign countries for pollination was included. The length of mt 16S rRNA sequence ranged from 496 bp to 508 bp and sequence divergence ranged from 1.4％ (7 bp) to 15.49％ (77bp). As expected, a high A＋T content was observed (78.5％ on average). According to the phylogeny tree derived from parsimony and maximum likelihood analysis, a monphyletic Bombus species, excluding a single cuckoo bumblebee, Psithyrus coreanus, was obtained, but the bootstrap estimate at the node supporting the monophyletic group was very weak (40％ or 46％), suggesting a very close relationship of the cuckoo bumblebee to the true bumblebee. Within Bombus species belonging to identical subgenera subgeneric specific clustering was formed with high bootstrap values, implying validity of the subgeneric names of each species: Pyrobombus for B. ardens and B. modeatus; Megabombus for B. consobrinus wittenburgi and B. koreanus; and Bombus s. str. for B. ignitus, B. hypocrita sapporoensis, and B. terrestris.