• Molecules and Cells
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• v.24 no.1
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• pp.125-131
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• 2007

von Willebrand factor (vWF) is a multimeric glycoprotein which functions within the coagulation system. It colocalizes with factor VIII (FVIII) by non-covalent interaction and alters its intracellular trafficking. vWF is also instrumental in maintaining the stability of secreted FVIII. The principal objective of this study was to generate a lentivirus-based vWF expression vector for gene therapy of hemophilia A. We inserted a vWF of 8.8 Kb into a lentiviral vector thereby producing VSV-G-pseudotyped vEx52. However, its titer was quite low, presumably because the length of vWF gene exceeds the size limit of the lentiviral vector. In order to overcome the low-titer, we concentrated the vEx52 and thus increased the efficiency of transduction approximately 6-fold with $1/100^{th}$ of the volume. However, as concentration requires an additional laborious step, we attempted to enhance the transduction efficiency by deleting exons 24-46 and 29-46 in pRex52 to construct pRex23 and pRex28, and in pvEx52, yielding pvEx23 and pvEx28, respectively. The transfected pRex52 had a profound effect on the activity of secreted FVIII, and this activity declined as domains of vWF were deleted. However, when the domain-deleted vWF-lentiviruses were transduced into K562 cells, the vEx28 increased the activity of the secreted FVIII compared to what was observed with vEx52. This result is probably due to higher efficiencies of transduction and expression while retaining the essential domains required for proper interaction with FVIII.

• Journal of the Institute of Electronics Engineers of Korea TE
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• v.37 no.5
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• pp.7-16
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• 2000

This paper described a design and implementation of the division/square-root for a redundant floating point binary number using high-speed quotient selector. This division/square-root used the method of a redundant binary addition with 25MHz clock speed. The addition of two numbers can be performed in a constant time independent of the word length since carry propagation can be eliminated. We have developed a 16-bit VLSI circuit for division and square-root operations used extensively in each iterative step. It performed the division and square-toot by a redundant binary addition to the shifted binary number every 16 cycles. Also the circuit uses the nonrestoring method to obtain a quotient. The quotient selection logic used a leading three digits of partial remainders in order to be implemented in a simple circuit. As a result, the performance of the proposed scheme is further enhanced in the speed of operation process by applying new quotient selection addition logic which can be parallelly process the quotient decision field. It showed the speed-up of 13% faster than previously presented schemes used the same algorithms.

• Journal of Fashion Business
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• v.10 no.4
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• pp.29-44
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• 2006

This study was aimed at giving help to the people intending to change hair fashion feeling for making their own hair style design and also providing the academic guide line to the cosmetic circles for developing new hair design and promoting sales by producing hair styles following the results from the statistical analysis based on the theoretical study on the face contour groups & hair fashion feeling groups. The researching methods were composed of following 3 steps ; prior theoretical research, statistical analysis, and hair style production. At first, the prior theoretical research was accomplished by analysis of literatures, magazines and internet sites about face contour, total & hair fashion feelings, hair style productions. Second, the prior statistical analysis were done about hairstyle images & their charateristics based on fashion feelings, and characteristics of fashion feeling group. And the third, hair style productions were done coordinated by face contours(oval, circle, long, square, reverse triangle) and hair fashion feelings(natural, sexy, sophisticate, ethnic, romantic pretty, elegance, sporty, avant garde) following the statistical results. But owing to the limitations to change hair length and color, these changes are modified by wigs and photoshop 7.0 program. So we could know there was no confirmed hair fashion feeling of one's best, but one could change one's hair fashion feeling and express one's beauty if one could adjust one's hair styles properly to one's face contour. This study would be very helpful to the people trying to change their own hair fashion feeling and be useful to the cosmetic circles for developing new design and promoting sales by comprehending hair design market and also be valuable to develop the methodology of 3 step research.

• Journal of Korean Society of Steel Construction
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• v.14 no.1
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• pp.95-104
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• 2002

In this paper, research for dynamic wheel loads of 3-D vehicle model considering tire enveloping model is carried out. Heavy trucks with 2-axles and 3-axles are modeled by 7-d.o.f. and 8-d.o.f., in which contact length of tire and pitching of tandem spring axles is considered. Dynamic equations of vehicle are derived by using the Lagrange's equation and solution of the equation is calculated by 5th Runge-Kutter method. The validity of the developed 3-D vehicle model is demonstrated by comparing the results obtained by the present method and experimental data by Whittemore. The maximum impact factors of tire force are calculated when vehicle models of 8ton and 15ton dump truck are running on the different class roads with 1.0km and on the various step bump.

• Journal of computational fluids engineering
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• v.17 no.3
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• pp.75-86
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• 2012

We developed a preliminary CFD analysis methodology to predict a pressure build up due to hydrogen flame acceleration in the APR1400 IRWST on the basis of CFD analysis results for test data of hydrogen flame acceleration in a scaled-down test facility performed by Korea Atomic Energy Research Institute. We found out that ANSYS CFX-13 with a combustion model of the so-called turbulent flame closure and a model constant of A = 5.0, a grid model with a hexahedral cell length of 5.0 mm, and a time step size of $1.0{\times}10^{-5}$ s can be a useful tool to predict the pressure build up due to the hydrogen flame acceleration in the test results. Through the comparison of the simulated results with the test results, we found out that the proposed CFD analysis methodology enables us to predict the peak pressure within an error range of about ${\pm}29%$ for the hydrogen concentration of 19.5%. However, the error ranges of the peak pressure for the hydrogen concentration of 15.4% and 18.6% were about 66% and 51%, respectively. To reduce the error ranges in case of the hydrogen concentration of 15.4% and 18.6%, some uncertainties of the test conditions should be clarified. In addition, an investigation for a possibility of flame extinction in the test results should be performed.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.5B
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• pp.313-320
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• 2012

A hybrid floating structure system combined with pontoon and semi-submersible type modules is proposed. This new system can reduce tensile forces of bottom slabs which could cause fatal damage of concrete floating structures. We performed a parametric study on the dimensions of this new system and investigate the sensitivity of the parameters to the behavior. In order to investigate various cases efficiently, we developed a simple two-step static analysis method for the fluid-structure interaction. An optimum system is derived from the investigation of the analysis results, weights and drafts of the hybrid structure. This study shows that introducing this new system to concrete floating structures is an effective way to reduce the tensile force of the bottm slab of such a floating structure. Also, it was found that when the length of the semi-submersible module is about 15%, the behavior would be optimal in the considered case.

• Journal of the Korea institute for structural maintenance and inspection
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• v.8 no.4
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• pp.175-183
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• 2004

The most important element is a rise ratio when regarding beauty and economics of arch bridges. Only the effect of dead load has been considered to decide the rise ratio. In this study, when going over the rise ratio of arch bridges, examined the problems, that the determination of the rise ratio by the dead load has, by adding the factor of a determination of optimum rise ratio, which is not only the effect of the dead load that has been currently considered but also the problem with respect to dynamic stability that is now taken seriously. Synthetically, when deciding rise ratio that is investigated in basic step of design, it is necessary to consider the evaluation dynamic stability.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.467-473
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• 2010

To improve the expression efficiency of recombinant endo-$\beta$-1,4-glucanase in P. pastoris, the endo-$\beta$-1,4-glucanase (egI) gene from Aspergillus niger was synthesized using optimized codons. Fourteen pairs of oligonucleotides with 15 bp overlap were designed and the full-length syn-egI gene was generated by two-step PCR-based DNA synthesis. In the synthesized endo-$\beta$-1,4-glucanase gene syn-egI, 193 nucleotides were changed, and the G+C content was decreased from 54% to 44.2%. The syn-egI gene was inserted into pPIC9K and transformed into P. pastoris GS115 by electroporation. The enzyme activity of recombinant P. pastoris stain 2-7# reached 20.3 U/ml with 1% barley $\beta$-glucan and 3.3 U/ml with 1% carboxymethylcellulose (CMC) as substrates in shake flasks versus 1,270.3 U/ml and 220.7 U/ml for the same substrates in 50-1 fermentors. The molecular mass of the recombinant protein was approximately 40 kDa as determined by SDS-PAGE analysis, the optimal temperature for recombinant enzyme activity was $70^{\circ}C$, and the optimal pH was 5.0 when CMC was used as the substrate.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.34 no.7
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• pp.79-88
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• 2006

In the present paper, the swirl flow structure and flame characteristics of turbulent premixed combustion in a model gas turbine combustor are investigated using large eddy simulation(LES). A G-equation flamelet model is employed to simulate the unsteady flame behavior. When inlet swirl number is increased, the distinct flow structures, such as the shapes of corner recirculation and center toroidal recirculation zone, are observed and the flame length is shorted gradually. Also, the phenomena of flashback are identified at strong swirl intensity. In order to get the accurate description of unsteady flame behavior, the predictive ability of the acoustic wave in a combustor is primarily evaluated. It is found that the vortex generated near the edge of step plays an important role in the flame fluctuation. Finally it is examined systematically that the flame and heat release fluctuation are coupled strongly to the vortex shedding generated by swirl flow and acoustic wave propagation from the analysis of flame-vortex interaction.

• Journal of Biomedical Engineering Research
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• v.41 no.3
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• pp.121-127
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• 2020

The purpose of this paper is to review the validation on the application of low frequency IMU(Inertial Measurement Unit) sensors by replacing high frequency motion analysis systems. Using an infrared-based 3D motion analysis system and IMU sensors (22 Hz) simultaneously, the gait cycle and knee flexion angle were measured. And the accuracy of each gait parameter was compared according to the statistical analysis method. The Bland-Altman plot analysis method was used to verify whether proper accuracy can be obtained when extracting gait parameters with low frequency sensors. As a result of the study, the use of the new gait assessment system was able to identify adequate accuracy in the measurement of cadence and stance phase. In addition, if the number of gait cycles is increased and the results of body anthropometric measurements are reflected in the gait analysis algorithm, is expected to improve accuracy in step length, walking speed, and range of motion measurements. The suggested gait assessment system is expected to make gait analysis more convenient. Furthermore, it will provide patients more accurate assessment and customized rehabilitation program through the quantitative data driven results.