• 미생물학회지
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• 제31권1호
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• pp.48-53
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• 1993

Saccharomyces distaticus 의 glucoamylase 생성능을 증진시킬 목적으로 STA1 유전자를 YIp vector 를 이용하여 염색체에 도입해 주고자 하였다. STA1 유전자 5.8-Kb 를 YIp vector 에 재조합하여 YIp-STA 를 재작하고, S. diastaticus GMT-11 (a, ura 3, STA1) 을 숙주균주로 하여 염색체의 STA1 유전자 부위에 homologous recombination 되어 삽입하도록 형질전환을 실시하였다. 이렇게 하여 glucoamylase 생성능이 모균주에 비해 최대 6배까지 증대된 다양한 형질전환체들을 얻을 수 있었다. 그리고 glycoamylase 생성능이 증대된 형질전환체들의 염색체 DNA 를 분리하여 Southern hybridization 을 실시한 결과 YIp-STA 가 multi-copy integration 되었음을 확인하였고, 또한 도입해 준 YIp-STA 는 세포분열인 30세대기간 동안 계속되었어도 안정하게 유지되었음을 알았다.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 연합학술대회
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• pp.125.1-125.1
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• 2003

• 한국농공학회논문집
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• 제49권5호
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• pp.11-20
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• 2007

In this study, we reviewed the optimal space for the fishway and fish storage establishment in the section between the downstream of the Hwacheon dam and the confluence of the Pungsan river. The hydraulic stability of these facilities was also examined. In the section between the downstream of Hwacheon dam and the confluence of Pungsan river, the release flow of Hwacheon dam was set at $5,495m^3/s,\;6,110m^3/s$ and $7,537m^3/s$, and numerical simulations were executed for each instance using the HEC-RAS model and RMA-2. In the analysis of the flow velocity distributions in the subject section by release flows of Hwacheon dam, it was shown that there was over 5.0m/s of high flow velocity at Sta. $#1{\sim}Sta.$ #3, Sta. $#4{\sim}Sta.$ #6, and Sta. $#12{\sim}Sta.$ #13 of the sections analyzed in this study. The Sta. $#0{\sim}Sta.$ #1 was considered appropriate for the fishway and fish storage establishment. As seen in the results of the numerical stability review by release flow conditions of Hwacheon dam subject to the selected available areas fur fishway and fish storage, the topographically available section for the establishment of fishway and fish storage exists at Sta. $#1{\sim}Sta.$ #3 when the release flow of Hwacheon dam is $5,495m^3/s$, whereas Sta. $#0{\sim}Sta.$ #1 should be reviewed for the subject section for the establishment of fishway at release flows of $6,110m^3/s$ and $7,537m^3/s$, in addition to the figures gathered at the $5,495m^3/s$ release flow. Finally, the available section for the establishment of fishway and fish storage was determined to be Sta. $#0{\sim}Sta.$ #3. Further, if fishway and fish storage are established in the outer bank within this section, the high flow velocity section should be avoided. An alternative would be to establish for the fishway in the inner band on the section of Sta. #1 or Sta. #3.

• 미생물학회지
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• 제30권5호
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• pp.403-409
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• 1992

STA1 유전자의 promoter 와 분비신호서열을 이용하여 B. subtilis 의 CMSase 를 분비하는 재조합 효모균주를 육성하였다. STA1A 유전자의 promoter, 분비신호서열, TS region 및 mature glucoamylase N-말단부위의 아미노산 98개와 B. subtilis 의 CMCase 구조유전자가 차례로 연결된 재조합플라스미드 pYESC24 를 제작한후 효모에 형질전환하였으나 CMCase 가 세포외로 분비되지 않았다. 반면에 STA1 의 TS region 및 mature glucoamylase N-말단 아미노산 98 개를 제거하여 CMMase 구조유전자갸 STA1 의 분비신호서열에 바로 연결된 재조합 플라스미드 pYESC11 에 의한 효모형질전환 균주는 CMCase 분비능이 아주 우수하였다. 이 형질전환 균주를 YPD 배지에서 4 일간 배양한 후 세포부위 별 CMCase 역가를 측정한 결과 배양액 1 m/당 총역가 44.7 unit 존재하였으며 이중 93% 이상이 배양상등액에서 관찰되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2000년도 춘계 학술대회지
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• pp.416-417
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• 2000

• 한국환경과학회지
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• 제4권5호
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• pp.103-103
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• 1995

In the Chinhae Bay, Korea, sedimentation rates and sedimentary record of anthropogenic metal loads were determined by $^{210}Pb$ dating and heavy metal analysis of four sediment cores . The sedimentation rates varied from 0.16g/$cm^2$/yr(3.1mm/yr) at Sta. Ct, located within narrow waterway to 0.24g/$cm^2$/yr(4.8mm/yr) at Sta. Cl, located in Haengam Bay. Maximum contents of Mn, Zn, Cu and Cr were observed at Sta. C2 located near the mouth of Masan Bay, while minimum contents were observed at Sta. CB. Mn/Fe ratios at Sta. C2 and Sta. C4 showed gradually increasing and decreasing downward, respectively, in the upper layer of sediment cores. This suggests that Mn may be diagenetically redistributed in highly reduced environment. At Sta. C2, the concentrations of Zn and Cu began to increase from 1920s by anthropogenic input and have been remarkablely increasing since mid 1960s. At Sta. C3, located near Sungpo, anthropogenic input of these two elements has also slightly increased after 1970s. However, pollution of these two elements was not significant in Haengam Bay(Sta. Cl) and Chiljun watenway(Sta. C4). The pollution of Co, Ni and Cr was not remarkable in all core samples except surface sediment of Sta. C2. The total input of anthropogenic Zn and Cu since 1920s was estimated to be 28∼792 ㎍/cm2 and 0∼168㎍/cm2, respectively. Sta. C2 showed remarkablely higher values relative to other stations: anthropogenic loads of Zn and Cu constituted 27% and 29% of the total sedimentary inventories at the present day, respectively. Fe, Ni, Cr and Co contents showed good correlation(r>0.8) with each other. Anthropogenic Zn and Cu also showed a very good positive correlation(>0.9). However, correlation between these two group of element was quite scattered, indicating different sources and geochemical behaviors.

• 한국환경과학회지
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• 제4권5호
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• pp.489-500
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• 1995

In the Chinhae Bay, Korea, sedimentation rates and sedimentary record of anthropogenic metal loads were determined by $^{210}Pb$ dating and heavy metal analysis of four sediment cores . The sedimentation rates varied from 0.16g/$cm^2$/yr(3.1mm/yr) at Sta. Ct, located within narrow waterway to 0.24g/$cm^2$/yr(4.8mm/yr) at Sta. Cl, located in Haengam Bay. Maximum contents of Mn, Zn, Cu and Cr were observed at Sta. C2 located near the mouth of Masan Bay, while minimum contents were observed at Sta. CB. Mn/Fe ratios at Sta. C2 and Sta. C4 showed gradually increasing and decreasing downward, respectively, in the upper layer of sediment cores. This suggests that Mn may be diagenetically redistributed in highly reduced environment. At Sta. C2, the concentrations of Zn and Cu began to increase from 1920s by anthropogenic input and have been remarkablely increasing since mid 1960s. At Sta. C3, located near Sungpo, anthropogenic input of these two elements has also slightly increased after 1970s. However, pollution of these two elements was not significant in Haengam Bay(Sta. Cl) and Chiljun watenway(Sta. C4). The pollution of Co, Ni and Cr was not remarkable in all core samples except surface sediment of Sta. C2. The total input of anthropogenic Zn and Cu since 1920s was estimated to be 28~792 $\mu\textrm{g}$/cm2 and 0~168$\mu\textrm{g}$/cm2, respectively. Sta. C2 showed remarkablely higher values relative to other stations: anthropogenic loads of Zn and Cu constituted 27% and 29% of the total sedimentary inventories at the present day, respectively. Fe, Ni, Cr and Co contents showed good correlation(r>0.8) with each other. Anthropogenic Zn and Cu also showed a very good positive correlation(>0.9). However, correlation between these two group of element was quite scattered, indicating different sources and geochemical behaviors.

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 1993년도 가을 학술논문발표요지
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• pp.7-8
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• 1993

1970년대의 오일쇼크는 시설재배 경영자로 하여금 성에너지를 효율적으로 이용케하는 연구에 박차를 가했다. 따라서 1970년대 후반에 남부지방에서는 지하수열을 이용하는 방법으로서 소위 Water curtain의 방법을 이용한 시설의 난방 방법이 넓게 확산되어갔다. Water curtain의 난방 방법은 통상 15$^{\circ}C$ 전후 수원의 지하수를 이용해 curtain 위에 살수함으로써 동기에 1$0^{\circ}C$ 정도의 온실의 유지가 가능하여 비교적 저염성 채소(토마토, 딸기 등)의 재배에 이용되고 있다. (중략)

• 시스템엔지니어링학술지
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• 제19권1호
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• pp.29-38
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• 2023

Independent Safety Assessment (ISA) is a third-party assessment that is to confirm that the system satisfies the safety requirements in the defined operational context. The process of this assessment often brings about many complex arguments that should be supported by evidence and justification. The communication between arguments and evidence is of the most importance in the context of safety case. This study illustrates how NOR-STA can be used for ISA process, showing the effective structure of safety compliance. The study outlines the steps to breaks down the top goal into many elements such as arguments, sub-goals, justification, context and assumptions. It concludes that the evidence-based safety conformance process utilizing NOR-STA provides a more effective and systematic representation of the independent safety assessment process in conformance cases.

• 융합보안논문지
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• 제11권1호
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• pp.33-38
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• 2011

현재 IEEE 802.11표준은 AP와 STA사이의 인증 및 보안 메커니즘이 취약하다고 많이 알려져 있다. 따라서, IEEE는 RSN(Robust Security Network)을 802.11에 대한 보안 아키텍처를 제안했다. RSN은 접근제어와, 인증, 그리고 키 관리 기반으로 IEEE 802.1X 표준을 사용한다. 본 논문에서는 IEEE 802.1X 또는, 802.11이 결합된 몇 가지 모델에 대한 취약점을 제시하고, 세션가로채기 또는 MiM(Man-in the-Middle)공격에 대응 할 수 있는 STA와 AP간의 접근제어, 인증 메커니즘을 설계하였다.