• Title/Summary/Keyword: SSRs

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Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences

  • Liu, Zhigao;Korpelainen, Helena
    • Horticulture, Environment, and Biotechnology : HEB
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    • v.59 no.6
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    • pp.889-897
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    • 2018
  • Currently, there is a lack of genetic markers capable of effectively detecting polymorphisms in Clematis. Therefore, we developed new markers to investigate inter- and intraspecific diversity in Clematis. Based on the complete chloroplast genome of Clematis terniflora, simple sequence repeats were explored and primer pairs were designed for all ten adequate repeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS region was sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markers that can distinguish different species and be used in different genetic analyses, including cultivar identification to assist the breeding of new ornamental cultivars.

The Sympathetic Skin Responses after Thoracic Sympathicotomy for Patients with Palmar Hyperhidrosis (수장부 다한증환자의 흉부 교감신경절단술후 교감신경 피부반응)

  • 김오곤;홍종면;이석재;홍장수;이광래;김상규
    • Journal of Chest Surgery
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    • v.32 no.6
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    • pp.579-583
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    • 1999
  • Background: Thoracic sympathicotomy has been used safely and successfully to manage palmar hyperhidrosis. The preoperative and postoperative recording of Sympathetic Skin Responses(SSR) was performed for objective evaluation and follow-up of thoracic sympathicotomy in hyperhidrosis patients, and also for ascertaining the clinical usefullness of SSR. Material and Method: The recording of SSR was performed on 15 patients suffering from palmar hyperhidrosis with Medelec Sapphire Plus electromyogragh before and after thoracic sympathicotomy. Eletrical stimuli on the right median nerve was made in patients in supine position and results were recorded on right and left palms with soles at the same time by 4 channels. Skin temperatures were also monitored simultaneously. T2,3 sympathicotomy was performed with VATS in every patients. SSR was done in 2 patients one month later. Result: Clinically, all patients had symptomatic improvement with satisfaction. Postoperative complication was small amount of residual pneumothorax in 5 patients but it was absorbed sponteneously. There was no recurrence during follow-up period and ten patients(66%) complained compensatory hyperhidrosis. After operation, SSR change was shown in every 15 patients. Abolition of SSR on both palms was achieved in 12 patients(80%) and on both soles in 6 patients. In the other 3 patients, the latencies were significantly delayed and the amplitudes were significantly reduced at both palms and soles. In two patients who were examined at one month later after operation, similar results with postoperative SSRs were shown. The skin temperature on preoperative both palm and sole were lower than normal temperature, and those on postoperative both palm and sole were increased. Those had statistical significance(p<0.05), and the temperature on the palm was increased higher that than on the sole. Conclusion: After thoracic sympathicotomy was performed on palmar hyperhidrosis patients, an increment of skin temperatures and SSR changes were achieved at both palms and soles of all patients. Palmar SSRs were completely abolished in 12 patients(80%), and similar results of postoperative SSRs were achieved. The recording of SSR may be useful to easily and objectively assess the completeness of sympathicotomy and the follow-up of recurrence in hyperhidrosis patients.

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Development of SSR markers for classification of Flammulina velutipes strains (팽이버섯 (Flammulina velutipes) 계통의 분류를 위한 SSR 마커개발)

  • Woo, Sung-I;Seo, Kyoung-In;Jang, Kab yeul;Kong, Won-Sik
    • Journal of Mushroom
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    • v.15 no.2
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    • pp.78-83
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    • 2017
  • Microsatellite SSR markers were developed and utilized to reveal the genetic diversity of 32 strains of Flammulina velutipes collected in Korea, China, and Japan. From the SSR-enriched library, 490 white colonies were randomly selected and sequenced. Among the 490 sequenced clones, 85 (17.35%) were redundant. Among the remaining 405 unique clones, 201 (49.6%) contained microsatellite sequences. We used 12 primer pairs that produced reproducible polymorphic bands for four diverse strains, and these selected markers were further characterized in 32 Flammulina velutipes strains. A total of 34 alleles were detected using the 12 markers, with an average of 3.42 alleles, and the number of alleles ranged from two to seven per locus. The major allele frequency ranged from 0.42 (GB-FV-127) to 0.98 (GB-FV-166), and values for observed ($H_O$) and expected ($H_E$) heterozygosity ranged from 0.00 to 0.94 (mean = 0.18) and from 0.03 to 0.67 (mean = 0.32), respectively. SSR loci amplified with GB-FV-127 markers gave the highest polymorphism information content (PIC) of 0.61 and mean allele number of five, whereas for loci amplified with GB-FV-166 markers these values were the lowest, namely 0.03 and two. The mean PIC value (0.29) observed in the present study with average number of alleles (3.42). The genetic relationships among the 32 Flammulina velutipes strains on the basis of SSR data were investigated by UPGMA cluster analysis. In conclusion, we succeeded in developing 12 polymorphic SSRs markers from an SSR-enriched library of Flammulina velutipes. These SSRs are presently being used for phylogenetic analysis and evaluation of genetic variations. In future, these SSR markers will be used in clarifying taxonomic relationships among the Flammulina velutipes.

Group Music Therapy Involving Creation of a Musical Play to Improve Self-Esteem, Self-Expression, and Social Skills in Children (음악극 창작활동이 지역아동센터 아동의 자아존중감, 자기표현력, 사회성에 미치는 영향)

  • Bae, Sungjin;Kim, Kyungsuk
    • Journal of Music and Human Behavior
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    • v.17 no.1
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    • pp.51-70
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    • 2020
  • The purpose of this study was to examine the effects of group music therapy involving the creation of a musical play on the self-esteem, self-expression, and social skills of children. The participants were 14 first through third graders and 14 fourth through sixth graders from a children's center. Half of the children from each grade band (Grades 1-3 and 4-6) were assigned to the experimental group and the other half to the control group, for a total of 14 children in each group. The experimental group who participated in music therapy sessions focused on creating a musical play. The experimental group selected themes, made a script, composed song lyrics and music, and performed the completed musical play. The Self-Esteem Scale, Self-Expression Scale, and Social Skills Rating System (SSRS) were administered before and after the intervention. The experimental group exhibited significantly higher scores than the control group on all of the scales except subscales of the Self-Expression scale and the SSRS. The results of this study suggest that group music therapy can facilitate children's engagement in group work and that playing an important role in the group can positively impact their perceptions of themselves.

Improvement of Basic Design Process for Submarines by Integration of SE-Based Technical Review and Requirements Management Process (SE 기반 기술검토 및 요구사항 관리 프로세스의 통합을 통한 잠수함 기본설계 프로세스의 개선)

  • Shin, Sung-Chul;Park, Jin-Won;Lee, Jae-Chon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.96-104
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    • 2018
  • Military vessels are complex weapon systems consisting of various integrated onboard equipment. Since their acquisition of military equipment takes a long period of time and a prototype ship is deployed and tested, vessel development has relied on its proprietary process. However, due to the growing complexity of onboard equipment technology, application of systems engineering (SE) process has become indispensable. Nonetheless, an effective design process complementing the existing design with the SE has not yet been developed. As such, we have studied an improved basic design process for submarines based on SE. To do so, we analyzed the processes for the basic design, technical review, and requirement management. Included reviews and requirements are SRR, SFR and PDR, and SSRS, SSS, and SSDD. By combining the results in the SE framework, we built an improved basic design process that can be applied in parallel with the SE-based technical review and requirement management. To assess the process, we applied our proposed model to the submarine development undergoing the basic design phase. It is possible to effectively manage the requirements, design artifacts and improve traceability, and also utilize them as test and evaluation materials. The SE-based basic design process is expected to be useful in other kinds of vessel design.

A Case Study on the Improvement of Sociality in Convergence Theater Education : Focusing on a Theatrical Camp Milmi-ri Village School (융합연극교육의 사회성 발달 증진 효과사례 분석 : 연극캠프 밀미리 마을학교를 중심으로)

  • Kim, Jung-Sun;Bae, Jin-Sup
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.6
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    • pp.221-233
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    • 2019
  • The purpose of this study tries to show the effectiveness of integrated theater education for low-grade students who lack social skills with the direction of art education based on 'theatrical and educational theater' and 'theater therapy' in the revised curriculum in 2015. To do this, the researcher organized a theater camp organized by the social, cultural and arts education institute in Seoul for a total of 12 low-grade elementary school students for four weeks, and had a presentation session. In addition, the researcher observed the behavioral changes of the participating children to verify the social development of the theater education and after the conclusion, the final results were obtained through the in-depth interviews of the participants' parents and teachers. As a result, it was found that Social Skills Rating System(SSRS)' test, teachers' observation logs, interviews, and responses of participating children, which are the main subjects of this activity, had a great effect on improving their sociality and relationship. Therefore, the researcher tried to prove through this paper that theater education is effective in enhancing social skills as creative, personality and convergence education in preparation for the 4th industrial era and that it is important to solve communication problems such as the absence of human relationships.

Analysis of the Genetic Diversity and Population Structure of Amaranth Accessions from South America Using 14 SSR Markers

  • Oo, Win Htet;Park, Yong-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.4
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    • pp.336-346
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    • 2013
  • Amaranth (Amaranthus sp. L.) is an important group of plants that includes grain, vegetable, and ornamental types. Centers of diversity for Amaranths are Central and South America, India, and South East Asia, with secondary centers of diversity in West and East Africa. The present study was performed to determine the genetic diversity and population structure of 75 amaranth accessions: 65 from South America and 10 from South Asia as controls using 14 SSR markers. Ninety-nine alleles were detected at an average of seven alleles per SSR locus. Model-based structure analysis revealed the presence of two subpopulations and 3 admixtures, which was consistent with clustering based on the genetic distance. The average major allele frequency and polymorphic information content (PIC) were 0.42 and 0.39, respectively. According to the model-based structure analysis based on genetic distance, 75 accessions (96%) were classified into two clusters, and only three accessions (4%) were admixtures. Cluster 1 had a higher allele number and PIC values than Cluster 2. Model-based structure analysis revealed the presence of two subpopulations and three admixtures in the 75 accessions. The results of this study provide effective information for future germplasm conservation and improvement programs in Amaranthus.

Effects of Social Skills Training Program for Children with Tendency of Attention-Deficit Hyperactivity Disorder (ADHD 경향 아동의 사회기술훈련 프로그램의 효과)

  • Lim, Yoon-Hee;Kim, Mi-Han;Choi, Yeon-Hee
    • Journal of the Korean Society of School Health
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    • v.23 no.2
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    • pp.237-245
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    • 2010
  • Purpose: The purpose of this thesis was to examine the effects of social skills training program onto the children with tendency of attention-deficit hyperactivity disorder. Methods: This study used nonequivalent control group pre/post-test quasi-experimental research design. The subjects were 18 children with tendency of attention- deficit hyperactivity in D City. The subjects were divided into two groups, an experimental group of 8 children and a control group of 10. The program consisted of 20 sessions of 60 minutes per session, 5 days a weeks, for 4 weeks. The research tools included Conner's Teacher Rating Scales (CTRS) and Social Skills Rating System (SSRS). The collected data were analyzed using $x^2$ test, Mann-Whitney test on the SPSS 17.0 program. Results: a) the scores for cooperation, self-assertiveness, self-control and empathy increased significantly in the experimental group, compared to the control group. b) the scores for social skills increased significantly in the experimental group, compared to the control group. Conclusion: It appears that the social skills training program is a useful nursing intervention to improve the social skills for children with tendency of attention-deficit hyperactivity.

Seismic Qualification Test for SSDM Hydraulic System of Research Reactor (연구용 원자로 이차정지구동장치 수력시스템의 내진검증)

  • Kim, Sanghaun;Kim, Gyeong-Ho;Sun, Jong-Oh;Cho, Yeong-Garp;Jung, Taeck-Hyung;Kim, Jung-Hyun;Lee, Kwan-Hee
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.12 no.1
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    • pp.23-29
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    • 2016
  • The Second Shutdown Drive Mechanism (SSDM) provides an alternate and independent means of reactor shutdown. The Second Shutdown Rods (SSRs) of SSDMs are poised at the top of the core by the hydraulic force driven from a hydraulic system during normal operation. The rods drop by gravity when a trip is commended by a Reactor Protection System, Alternate Protection System, Automatic Seismic Trip System or operator by means of power off solenoid valves of hydraulic system. This paper describes the test results of seismic qualification of a prototype SSDM hydraulic system to demonstrate that its structural integrity and operability (functionality) are maintained during and after seismic excitations, that is, an adequacy of the SSDM design. From the results, this paper shows that the SSDM hydraulic system satisfies all its design requirements without any malfunctions during and after seismic excitations.

A Fast MSRCR Algorithm Using Hierarchical Discrete Correlation (HDC를 이용한 고속 MSRCR 알고리즘)

  • Han, Kyu-Phil
    • Journal of Korea Multimedia Society
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    • v.13 no.11
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    • pp.1621-1629
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    • 2010
  • This paper presents an improved fast MSRCR algorithm that MSRs are commonly adopted at tone mapping in color vision. Conventional MSRs consist of three SSRs, which use three Gaussian functions with different scales as those surround ones. This convolution processes require much computation load. Therefore, the proposed algorithm adopts a hierarchical discrete correlation which is equivalent to Gaussian function and the Retinex process is only applied to the luminance channel in order to get a fast processing. A simple color preservation scheme is applied to the Retinex output from the luminance channel in the proposed MSRCR algorithm. Experimental results show that the proposed algorithm required less number of oprations and computation time about 1/9.5 and 1/3.5 times, respectively, than those of the simplest MSR and was equivalent to conventional MSRs.