• Applied Chemistry for Engineering
• /
• v.23 no.3
• /
• pp.326-332
• /
• 2012

Among the samples prepared by various pre-treatment methods, the one pretreated by dilute sulfuric acid showed the highest glucose yield in the enzymatic hydrolysis. Statistical analysis of enzymatic hydrolysis revealed that the glucose yield was in proportion to the enzyme dosage, the ratio of the pre-treated sample to the buffer solution, and the reaction time and that the effect of enzyme dosage was predominant in the experiment range. In addition, the glucose yield was estimated to be 76.1% at an optimal enzymatic hydrolysis condition. In a separate hydrolysis and fermentation (SHF), Saccharomyces cerevisiae converted over 80% of glucose from the enzymatic hydrolysis of pre-treated wasted corn stalk by dilute sulfuric acid to bioethanol with 37% of ethanol yield and 0.42 $g/L{\cdot}hr$ of productivity. In the simultaneous saccharification and fermentation (SSF), 59.5% of conversion from glucan to ethanol and 0.20 $g/L{\cdot}hr$ of productivity were achieved. In both SHF and SSF, approximately 88 g of bioethanol could be obtained from 1 kg of wasted corn stalk. The possible amount of bioethanol in Gangwon province were estimated to be 1.9 kiloton with the assumption of the 50% of collection ratio.

• KSBB Journal
• /
• v.23 no.6
• /
• pp.499-503
• /
• 2008

The bioethanol for use as a liquid fuel by fermentation of renewable biomass as an alternative to petroleum is important from the viewpoint of global environmental protection. Recently, many scientists have attempted to increase the productivity of bioethanol process by developing specific microorganism as well as optimizing the process conditions. In the present study, which is based on our previous investigation on the pretreatment process, theproductivity of bioethanol obtained from simultaneous saccharification and fermentation (SSF) process was compared between various domestic materials including barley, brown rice, corn and sweet potato. Additionally, Solid glucoamylase (SGA; developed in Changhae Co.), from modified strain with UV, was used. The result was compared to commercial glucoamylase (GA). It was observed that the fermentation rate was increased together with the yield which can be derived from the final ethanol concentration. Especially, in the case of brown rice, compared to the experimental results using GA, the final ethanol concentration was 1.25 times higher and 18.4 g/L of the yield was increased. Also, the time required for reaching 95% of the maximum ethanol concentration is significantly reduced, which is approximately 36 hours, compared to 88 hours using GA. It means that SGA has excellent saccharogenic power.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.35 no.2
• /
• pp.131-140
• /
• 2022

Installing Continuous Welded Rail (CWR) is one of the economical ways to resolve the challenges of noise, vibration, and the open-deck steel railway bridge impact, and the SSF method using the interlocking sleeper fastener has recently been developed. In this study, the method employed for determining the optimum vertical stiffness of the sleeper pad installed under the bridge sleeper, which is utilized to adjust the rail height and absorb shock when the train passes when the interlocking sleeper fastener is applied, is presented. To determine the optimal vertical stiffness of the sleeper pad, related existing design codes are reviewed, and, running safety, ride comfort, track safety, and bridge vibration according to the change in the vertical stiffness of the sleeper pad are estimated via flexible multi-body dynamic analysis,. The flexible multi-body dynamic analysis is performed using commercial programs ABAQUS and VI-Rail. The numerical analysis is conducted using the bridge model for a 30m-long plate girder bridge, and the response is calculated when passing ITX Saemaeul and KTX vehicles and freight wagon when the vertical stiffness of the sleeper pad is altered from 7.5 kN/mm to 240 kN/mm. The optimum stiffness of the sleeper pad is calculated as 200 kN/mm under the conditions of the track components applied to the numerical analysis.

• KSBB Journal
• /
• v.11 no.3
• /
• pp.347-352
• /
• 1996

The enzyme deactivation in a membrane recycling system for the simultaneous saccharification and fermentation(SSF) was studied under various temperature and pressure. The optimum molecular weight cut off(MWCO) of the ultrafiltration membrane for recycling cellulase and ${\beta}$-glucosidase was 50,000. When the cellulase was recycled continuously through the membrane system, it was not deactivated. But the activity of ${\beta}$-glucosidase was decreased with an increase in operating temperature and transmembrane pressure. After 720 minutes at $42^{\circ}C$ and 24.8 psig , the activity of ${\beta}$-glucosidase was reduced by 35% of the initial activity. Such tendencies could be well explained by the results of highly induced shear at the fiber surface of membrane when temperature and transmembrane pressure became higher.

• Journal of environmental and Sanitary engineering
• /
• v.21 no.4 s.62
• /
• pp.61-76
• /
• 2006

A Line Density algorithm was developed to quantify the sea surface temperature distribution using NOAA Sea Surface Temperature(SST) data and Geographic Information Systems(GIS), In addition, a GIS based automation model was designed to extract the Line Density Indices were determined by applying K-means Cluster. SST data in terms of March to May obtained on the coastal area of the Uljin from 2001 to 2004 in spring were used to make two data sets of average sea water temperature map in terms of year as well as month. From the result it was formed that water temperature gradient in April was the strongest among the other months, In particular very strog formation of oceanic front as well as temperature gradients were observed in front of the coastal area around Wonduk and Jukbyeon countries. Because those coastal area is a confront zone of two cold and a warm. It is expected that the development of a Line Density Algorithm would contribute to quantify of the SST for the research of Sea Surface Front(SSF) related to marine life management and the sea environmental conservation.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
• /
• 2004.10a
• /
• pp.292-297
• /
• 2004

The conventional computerized numerical controller (CNC) of machine tools has been increasingly replaced by a PC-based open architecture CNC (OAC) which is independent of the CNC vendor. The OAC and machine tools with OAC led the convenient environment where it is possible to implement user-defined application programs efficiently within CNC. Tis paper proposes a method of operational fault cause diagnosis which is based on the status of programmable logic controller (PLC) in machine tools with OAC. The operational fault is defined as a disability state occurring during normal operation of machine tools. The faults are occupied by over 70% of all faults and are also unpredictable as most of them occur without any warning. Two diagnosis models, the switching function (SF) and the step switching function (SSF), are propose in order to diagnose the fault cause quickly and exactly. The cause of an occurring fault is logically diagnosed through a fault diagnosis system (FDS) using the diagnosis models. A suitable interface environment between CNC and develope application modules is constructed in order to implement the diagnostic functions in the CNC domain. The diagnosed results were displayed on a CNC monitor for machine operators and provided to a remote site through a web browser. The result of his research could be a model of the fault cause diagnosis and the remote monitoring for machine tools with OAC.

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2005.06a
• /
• pp.204-211
• /
• 2005

The technology of Solid-State-Fermentation (SSF) with corn straw by Pichia ohmeri T021 was studied in this article. After being crushed, the corn straw powder was added into vitriol solutions to hydrolysis, which the quality proportion of corn straw: water: vitriol (98%) is 20:80:1. The mixtures was incubated at 120$^{\circ}C$ for 1 hour, and the hydrolysis rate reached 19%. Following, the mixture was adjusted to pH 4.5 by sodium carbonate and added cellulase (25IFPU/g). The hydrolysis rate reached 15% after the mixture was incubated at 50$^{\circ}C$ for 25h. The mixture which hydrolysed by vitriol was inoculated by Pichia ohmeri T021 (5${\times}10^7$cell/g) and added cellulase (25 IFPU/g) at the same time. The ethanol yield reached 2.99g per 100 gram substrate after the fermenting grains was incubated at 33$^{\circ}C$, pH 4.5 for 5 days.

• KSBB Journal
• /
• v.5 no.4
• /
• pp.335-339
• /
• 1990

In order to develop economic processes for ethanol production from starch, a simultaneous saccharification and fermentation(SSF) process using Zymomonas mobilis and amyloglucosidase (AMG) was studied in semibatch modes using cell recycle. The cell recycle was carried out by adopting two different methods; microfiltration and settling. The cell recycle using microfiltration revealed higher productivity(5.4 g/l/h) than that using a settler(4.3 g/l/h). Taking the large-scale ethanol fermentation into account, the semibatch process using microfiltration system appeared most promising among others with respect to ethanol productivity, feasibility of scale-up and simplification of operation.

• International Journal of Control, Automation, and Systems
• /
• v.4 no.5
• /
• pp.637-644
• /
• 2006

Biomedical image is often complex. An applied image analysis system should deal with the images which are of quite low quality and are challenging to segment. This paper presents a framework for color cell image segmentation by learning and classification online. It is a robust two-stage scheme using kernel method and watershed transform. In first stage, a two-class SVM is employed to discriminate the pixels of object from background; where the SVM is trained on the data which has been analyzed using the mean shift procedure. A real-time training strategy is also developed for SVM. In second stage, as the post-processing, local watershed transform is used to separate clustering cells. Comparison with the SSF (Scale space filter) and classical watershed-based algorithm (those are often employed for cell image segmentation) is given. Experimental results demonstrate that the new method is more accurate and robust than compared methods.

• Communications for Statistical Applications and Methods
• /
• v.17 no.2
• /
• pp.263-273
• /
• 2010

Missing values in time series can be treated as unknown parameters and estimated by maximum likelihood or as random variables and predicted by the conditional expectation of the unknown values given the data. The purpose of this study is to impute missing values which are regarded as the maximum likelihood estimator and random variable in incomplete data and to compare with two methods using ARMA and STAR model. For illustration, the Mumps data reported from the national capital region monthly over the years 2001~2009 are used, and estimate precision of missing values and forecast precision of future data are compared with two methods.