• Title/Summary/Keyword: SSC13

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A Whole Genome Association Study on Meat Quality Traits Using High Density SNP Chips in a Cross between Korean Native Pig and Landrace

  • Lee, K.T.;Lee, Y.M.;Alam, M.;Choi, B.H.;Park, M.R.;Kim, K.S.;Kim, T.H.;Kim, Jong-Joo
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.11
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    • pp.1529-1539
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    • 2012
  • A whole genome association (WGA) study was performed to detect significant polymorphisms for meat quality traits in an $F_2$ cross population (N = 478) that were generated with Korean native pig sires and Landrace dams in National Livestock Research Institute, Songwhan, Korea. The animals were genotyped using Illumina porcine 60k SNP beadchips, in which a set of 46,865 SNPs were available for the WGA analyses on ten carcass quality traits; live weight, crude protein, crude lipids, crude ash, water holding capacity, drip loss, shear force, CIE L, CIE a and CIE b. Phenotypes were regressed on additive and dominance effects for each SNP using a simple linear regression model, after adjusting for sex, sire and slaughter stage as fixed effects. With the significant SNPs for each trait (p<0.001), a stepwise regression procedure was applied to determine the best set of SNPs with the additive and/or dominance effects. A total of 106 SNPs, or quantitative trait loci (QTL) were detected, and about 32 to 66% of the total phenotypic variation was explained by the significant SNPs for each trait. The QTL were identified in most porcine chromosomes (SSCs), in which majority of the QTL were detected in SSCs 1, 2, 12, 13, 14 and 16. Several QTL clusters were identified on SSCs 12, 16 and 17, and a cluster of QTL influencing crude protein, crude lipid, drip loss, shear force, CIE a and CIE b were located between 20 and 29 Mb of SSC12. A pleiotropic QTL for drip loss, CIE L and CIE b was also detected on SSC16. These QTL need to be validated in commercial pig populations for genetic improvement in meat quality via marker-assisted selection.

Effects on Quality of Strawberry Fruit by Dipping of Calcium Solution and MA Packaging (칼슘처리와 MA포장이 딸기의 품질에 미치는 영향)

  • Guan Junfeng;Park Hyung-Woo;Kim Yoon-Ho;Kim Sang-Hee;Park Hye-Ran
    • Food Science and Preservation
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    • v.13 no.1
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    • pp.13-17
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    • 2006
  • The strawberry was dipped into $1\%\;CaCl_2$ solution at $20^{\circ}C\;and\;45^{\circ}C$ for 15min. respectively, and then packaged with or without PE film (thickness: 0.011mm) before putting into the cold storage at $5^{\circ}C$. The results showed the lowest rate of rot incidence and the highest firmness with higher Vitamin C content and the best superficial appearance in the treatment of $CaCl_2$ dipping at $45^{\circ}C$ with packaging. It also showed lower pH and higher titratable acidity in the treatment However, no significant difference of SSC was found among various treatments. The results suggested that the combination of postharvest calcium dipping and packaging could result in more effective preservation for strawberry compared with the individual treatment.

Measurement of Volatile Organic Sulfur Compounds in Ambient Air by Canister and Tedlar-bag Sampling Method (캐니스터와 Tedlar-bag 시료채집법을 이용한 대기 중의 휘발성유기황화합물의 측정)

  • 전선주;허귀석
    • Journal of Korean Society for Atmospheric Environment
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    • v.15 no.4
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    • pp.417-428
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    • 1999
  • The measurement of VOSCs(volatile organic sulfur compounds) in the air is nowadays a very important environmental research field. It is, however, very difficult because the concentration of the VOSC in ambient air is usually very low and the high reactivity makes it difficult to keep in container without loss of recovery. In this study, sampling method with cryogenic preconcentration is evaluated for analysis of atmospheric VOSC such as $CH_3SH,\;CH_3CH_2SH,\;CH_3SCH_3,\;CS_2,\;CH_3SSCH_3,\;CH_3SCH_2SCHA_3,\;and\;C_2H_5SSC_2H_5$ analyzed by GC-MS or GC-FID. Repeatabilities of measurement accompanied with preconcentration for 3-successive runs were in the range of 0.2~1.0% as a relative standard deviation. Stabilities up to 13 days were measured in 6 L canister and 10 L tedlar bag filled with VOSCs in ppb level. Higher stability was observed in tedlar bag as compared to canister with glass coated inner walls, and thiol compounds show dramatic losses in canister within 2~3 days. It is found that recovery over 70% was obtained in a week for all tested VOSCs when the compounds from ambient air matrix were stored in tedlar bag. It is also found that the stabilities of VOSCs are depending on humidity and coexisting compounds in matrix gas due to sample adsorption onto inner surface and reactivity. The results indicate the possibility and limitations of VOSC analysis in ambient air using container sampling method with cryogenic preconcentration.

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Molecular Characterization, Chromosomal Localizations, Expression Profile, and Association Analysis of the Porcine PECI Gene with Carcass Traits

  • Gao, H.;Fan, B.;Zhu, M.J.;Liu, Bang
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.1
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    • pp.7-12
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    • 2010
  • The full-length cDNA of the porcine peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase (PECI) gene encodes a monofunctional peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase. Cloning and sequencing of the porcine PECI cDNA revealed the presence of an 1185-base pair open reading frame predicted to encode a 394-amino acid protein by the 5'rapid amplification of cDNA ends (5'RACE) and EST sequences. The porcine PECI gene was expressed in seven tissues (heart, liver, spleen, lung, kidney, skeletal muscle, fat) which was revealed by reverse transcriptase-polymerase chain reaction (RT-PCR). The porcine PECI was mapped to SSC71/2 p11-13 using the somatic cell hybrid panel (SCHP) and the radiation hybrid panel (RH) (LOD score 12.84). The data showed that PECI was closely linked to marker S0383. A C/T single nucleotide polymorphism in PECI exon 10 (3'UTR) was detected as a PvuII PCR-RFLP. Association analysis in our experimental pig population showed that different genotypes of PECI gene were significantly associated with the Average Backfat thickness (ABF) (p<0.05) and Buttock backfat thickness (p<0.01).

Effect of Quality Change of 'Fuji' Apple by Pressure Cooling (차압예냉 처리가 '후지' 사과의 품질 변화에 미치는 영향)

  • Park Hyung-Woo;Kim Sang-Hee;Cha Hwan-Soo;Kim Yoon-Ho;Choi Jee-Young
    • Food Science and Preservation
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    • v.13 no.4
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    • pp.427-431
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    • 2006
  • The research was conducted to measure the effect of pressure cooling of 'Fuji' apple during of 24 weeks. Weight loss in pressure cooling was lower than control at 24th week, and firmness in pressure cooling was higher than control. Titratable acidity in control and pressure cooling was 0.0823% 0.1103% in 24 weeks, respectively. Soluble solid content (SSC) in pressure cooling were higher than control. Decayed rate in control and pressure cooling was 46.4% 34.5% in 24 weeks, respectively. Total ascorbic acid contents were decreased in control and pressure cooling during 24 weeks. Therefore, it could be suggested that pressure cooling is more effective than control in storage of 'Fuji' apple.

Turbidity One-System for River and Reservoir (하천-저수지 탁수 일원화 모델 )

  • Seokil Jeong;Eun Ji Kim;Cheol Seo Ki;;EJae Kwon;Hwa Young Kim
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.510-510
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    • 2023
  • 집중호우의 증가는 저수지 내 탁수의 증가로 이어지며, 저수지에서의 탁수 배제는 하천의 탁수 문제로 연결될 수 있다. 탁수의 발생은 정수처리 비용 증가와 하천 생태계 문제 등을 발생시키므로, 이에 대한 해결책이 지속적으로 요구되고 있다. 특히 낙동강 유역의 임하호는 태풍 및 집중호우 발생 시 고탁수의 유입 빈도가 높다. 탁수가 임하호에 유입되면 일반적으로 저수지 탁수 예측 수치해석을 수행하여 탁수의 저수지 내 탁수 거동과 방류량에 따른 탁수 저감 정도를 파악하고, 탁수 배제를 위한 저수지 운영계획을 수립한다. 그러나 방류된 고탁수의 하천 거동에 대한 수치해석은 국내에서는 수행하고 있지 않은 실정이다. 임하댐 하류에서 내성천합류부까지는 약 0.325 m3/s의 물을 취수하고 있으므로(2022년 기준), 하천의 탁수도 저수지 탁수와 동시에 고려되어야 할 필요가 있다. 저수지와 하천의 유사 거동 분석에 대해 격자 구성 등의 이유로 일반적으로 각각 다른 tool을 사용하고 있으나, 모델구축 및 입□출력 data의 변환 등 구축, 구동, 분석 과정 모두 효율적이지 못하다. 이에 본 연구에서는 하천과 저수지에서의 탁수 거동을 동시에 예측할 수 있는 일원화된 수치해석 모델을 구축(임하호~낙본C)하였다. 준3차원 모델인 EFDC를 이용하였으며, 격자는 저수지는 직사각격자, 하천은 Curvlinear를 적용하였다. 연직방향으로는 저수지와 하천의 수심 차이가 크므로 EFDC 내에 탑재되어 있는 𝜎-z(uniform)레이어 옵션을 선택하여, 저수지 연직 레이어는 40개, 하천은 2개로 구축되었다. 하천에서의 탁수 거동 예측은 지류의 유량, 탁도 등의 경계조건 data를 얻기가 쉽지 않기 때문에, 유량은 임하호 유입유량에 유역면적비를 적용하였으며, 탁도는 TSS(Total Suspended Solid)-SSC(Suspended Sediment Concentration)-유량의 관계를 각각 회귀분석 등을 통해 도출하여 연구 범위 내 7개 지류하천에 적용하였다. 모의 시기는 2개의 태풍으로 연속적인 탁수가 발생한 2020년 08~10월로 결정하였다. 모의 결과 저수지와 하천의 PBIAS는 각각 13.6과 8.4로(Very Good 등급) 하천과 저수지를 동시에 모의하는 탁수 예측의 적용 가능성은 충분한 것으로 확인되었다. 그러나 이벤트별 지류의 유량과 탁도는 일정한 관계로 계산될 수 없으므로, 향후 유역 모델과 연계하여 지류 유입 유량 및 탁도의 정확도를 개선할 것이다.

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Effects of Elevated Spring Temperatures on the Growth and Fruit Quality of the Mandarin Hybrid 'Shiranuhi' (봄철 가온처리가 부지화의 생장과 과실품질에 미치는 영향)

  • Moon, Young-Eel;Kang, Seok-Beom;Han, Seung-Gab;Kim, Yong-Ho;Choi, Young-Hun;Koh, Seok Chan;Oh, Soonja
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.459-469
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    • 2015
  • The effects of elevated spring temperatures on the growth and fruit quality of the mandarin hybrid 'Shiranuhi' [(Citrus unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulata] were investigated in plastic greenhouses, to develop a cropping system to improve the quality of the fruit and increase the income of growers on Jeju Island, South Korea. Under conditions of elevated temperature I ($25/15^{\circ}C$, day/night) and elevated temperature II ($28/18^{\circ}C$, day/night) during early spring, budburst was advanced by 11 and 15 d, and full bloom by 22 and 45 d, respectively, compared to those of the plants grown at ambient air temperature in a plastic greenhouse. Elevated temperatures decreased the number of spring shoots but increased mean spring shoot length and leaf area. Growing 'Shiranuhi' trees at elevated temperatures resulted in increases in mean fruit weight and fruit L/D ratio (> 1.0). In addition, fruit color development was significantly advanced in trees grown under elevated temperatures during early spring, which allowed the fruit to be harvested 1-2 months earlier than trees grown under ambient air temperature. Fruit soluble solids content (SSC) and titratable acidity (TA) at harvest were similar between elevated temperature I and ambient air temperature, but were significantly higher than at elevated temperature II. Considering fruit quality, harvest time, and yield, the elevated temperature treatment regime of $25/15^{\circ}C$ (day/night) during early spring could be useful for cultivation of the mandarin hybrid 'Shiranuhi' to increase the income of growers.

Postharvest Quality Changes of Kimchi Cabbage 'Choongwang' Cultivar as Influenced by Postharvest Treatments (저장 전처리 방법에 따른 배추 '춘광' 품종의 품질변화)

  • Eum, Hyang Lan;Bae, Sang Jun;Kim, Byung-Sup;Yoon, Jungro;Kim, Jongkee;Hong, Sae Jin
    • Horticultural Science & Technology
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    • v.31 no.4
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    • pp.429-436
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    • 2013
  • Kimchi cabbage 'Choongwang' cultivar is mainly cultivated during summer in Gangneung area. 'Choongwang' cultivar was harvested in late July, applied with predrying, room cooling, and forced air cooling, and then packaged with/without 0.02 mm HDPE film to estimate the effect of postharvest treatment on quality characteristics (weight loss, trimming loss, firmness, SSC, color index, sensory evaluation) during 8 week storage at $2^{\circ}C$. Kimchi cabbage without 0.02 mm HDPE film showed high weight loss up to 13-20% while those of with liner were significantly lower. Also forced air cooling among the postharvest treatments was effective to reduce both weight loss and trimming loss. Appearance and freshness in sensory evaluation were the important factors in estimating good quality during storage. Liner treatment with forced air cooling showed highly significant for maintaining appearance and freshness ($P{\leq}0.01$). Color index was no differences between with/without 0.02 mm HDPE film and postharvest treatments. After 6 weeks storage in without 0.02 mm HDPE film with room cooling or control appearance was severely damaged and also internal browning was found. While in with 0.02 mm HDPE film internal browning was found after 8 weeks storage, just in room cooling or predrying treatment.

The Comparison of Quality Characteristics of Dried Persimmons Manufactured by Solar Drying Method in Yecheon (예천지역 천일건조 곶감의 품종별 품질특성 비교)

  • Park, Hyo-Won;Oh, Sung-Il;Cho, Mun-Gyeong;Kim, Chul-Woo;Lee, Uk
    • Journal of Korean Society of Forest Science
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    • v.107 no.4
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    • pp.422-427
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    • 2018
  • This study was performed to determine quality characteristics of solar-dried persimmon for each cultivar. The physical and sensory characteristics of 4 cultivars of solar-dried persimmons (Gojongsi, Godongsi, Sangjudungsi, Koshuhyakume) were investigated. As a result, yield rate and firmness was highest in Koshuhyakume (29.5%, 9.62 N). Meanwhile, the soluble solid content (SSC) was the highest in Gojongsi ($27.3^{\circ}Brix$) and it has a significant difference between 4 cultivars (p<0.05). The water activity was range from 0.784 to 0.819 and Gojongsi (0.784) was significantly lower than others. The water contents value was the highest in Koshuhyakume (37.51%), while Sangjudungsi (35.32%) was the lowest. In case of Hunter's value, 'L', 'a' and 'b' were the highest in Sangjudungsi (34.56, 9.24, 17.07), whereas Koshuhyakume (29.17, 5.68, 13.59) has generally low value. In sensory evaluation, overall acceptability was the highest in Gojongsi and Godongsi (5.16), while Koshuhyakume (3.95) was the lowest. The results showed that the quality characteristics of dried persimmon manufactured by solar drying method have distinct difference according to cultivars.

Sequencing, Genomic Structure, Chromosomal Mapping and Association Study of the Porcine ADAMTS1 Gene with Litter Size

  • Yue, K.;Peng, J.;Zheng, R.;Li, J.L.;Chen, J.F.;Li, F.E.;Dai, L.H.;Ding, SH.H.;Guo, W.H.;Xu, N.Y.;Xiong, Y.ZH.;Jiang, S.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.7
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    • pp.917-922
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    • 2008
  • A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTS1 gene was obtained. Analysis of the ADAMTS1 gene using the porcine radiation hybrid panel indicated that pig ADAMTS1 is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTS1 as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).