• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.19-19
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• 2023

제비꽃속 34분류군의 61개체를 대상으로 엽록체 DNA 게놈 특이 유전자의 특징을 알아보고자 하였다. 61개체의 엽록체 게놈 전체 길이는 155,535~158,940 bp 로 모두 전형적인 사분할 구조였다. 지역별로는 LSC 지역이 84,826~87,250 bp, SSC 지역이 16,338~18,654 bp, 그리고 IR 지역이 26,029~27,192 bp 였다. 유전자 개수는 131개로 84개 protein coding-gene, 37개 tRNA 유전자, 8개 rRNA유전자, 그리고 2개의 유사유전자인 𝜓rps19, 𝜓ycf1으로 구성되어 있었다. LSC/IRa 경계에 위치한 rps19 유전자 길이는 279 bp로 모든 분류군에서 동일하였으며, 𝜓rps19의 길이는 다양했으나 유전자 개수에는 영향을 미치지 않았다. SSC/IRb 경계에 위치한 ycf1 유전자 길이는 약 5,600 bp 였으나, V. japonica (MZ151699) 1개체에서는 다른 종에 비해 약 1,000 bp 위치에서 발생한 점돌연변이로 인해 종결 코돈이 나타나는 특징을 보였다. 한편 13분류군의 23개체에서는 𝜓ycf1의 길이가 650 bp 정도 짧은 것을 확인하였는데, 이 종류들은 원예종인 V. tricolor (ON262802) 이외에는 모두 줄기가 없는 분류군들로 IR 지역의 확장과 SSC 지역의 수축에 의한 것으로 판단된다. ndhF는 대체로 SSC 지역에 위치하나, V. inconspicua (MZ065354), V. mongolica (MW802534, ON548135), V. yunnanfuensis (MW802541) 등 4개체에서는 IRa/SSC 경계에 위치하면서 유사유전자가 발생하였고, 그 결과 다른 제비꽃 종류에 비해 유전자 개수가 132개로 차이를 보였다. 또한, V. collina (OP271831), V. mirabilis (MH256000), V. tricolor (ON262802) 등 3분류군에서는 SSC 지역이 inversion 되어 엽록체 이성질체가 존재함을 확인하였다. 이상의 결과를 종합하면, 제비 꽃속 엽록체 게놈 61개체의 ycf1, 𝜓ycf1, ndhF, 𝜓ndhF 등은 유전자 길이와 개수 등에 차이를 보이는 것으로 나타났으며, 제비꽃속에서도 엽록체 이성질체가 존재함을 확인할 수 있었다.

• Food Science and Preservation
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• v.23 no.7
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• pp.1058-1064
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• 2016

This study investigated the correlation between physicochemical (color, soluble solids content (SSC), pH, titratable acidity (TA), and firmness) and sensory (appearance, taste, odor, and texture) characteristics of environment-friendly 'Campbell early' grapes to identify quality indices. For analysis, samples of similar-sized grapes were collected from five orchards. The results showed that the physicochemical characteristics of CIE $L^*$, CIE $a^*/b^*$, SSC, pH, TA, and firmness and the sensorial characteristics of color intensity, freshness of stem, odor, sourness, sweetness, and elasticity were different among groups. Correlation analysis results showed that an increase in sweetness and firmness and a decrease in sourness were associated with an increase in overall acceptance. Sourness and sweetness were positively correlated with CIE $L^*$ (r=0.88) and firmness (r=0.95), individually. In the principal component analysis results, component F1 and F2 explained 44.35% and 33.77%, respectively, of the total variance (78.12%). F1 represented firmness, sweetness, elasticity, hardness, grape odor, color intensity, sweet odor, sourness, and damage degree. F2 represented CIE $L^*$, TA, CIE $a^*$, CIE $a^*/b^*$, SSC/TA, SSC, and peel thickness. The results showed that consumer acceptance of 'Campbell early' grapes can be determined by assessing physicochemical attributes of firmness, CIE $L^*$, TA, CIE $a^*$, CIE $a^*/b^*$, SSC/TA, and SSC and various sensorial attributes including sweetness, fruit elasticity, fruit hardness, grape odor, and color intensity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.256-261
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• 2017

The effects of temperature and length of cold storage on the quality maintenance of fresh "Gold" kiwifruit were investigated. Physio-chemical properties were analyzed in kiwifruit held at $2^{\circ}C$ and $6^{\circ}C$ temperatures compared to fruit at room temperature ($20{\sim}28^{\circ}C$) during 8 weeks of storage. Low temperatures ($2^{\circ}C$ and $6^{\circ}C$) significantly delayed softening and soluble solids content (SSC) accumulation compared to higher temperature ($20{\sim}28^{\circ}C$). Physico-chemical properties of fruits, including weight losses, firmness, SSC, titratable acidity (TA), SSC/TA ratio, and flesh color properties were monitored during storage. Fast firmness loss was detected in fruit stored at higher temperatures compared to low temperature ($2^{\circ}C$). Similar results were observed for acidity according to storage temperature and length of cold storage, whereas SSC increased to the limited values (%Brix) during storage. The soluble solids content (SSC) increased markedly during the first 60 days of storage and remained almost constant thereafter for all treatments. SSC accumulation rates decreased from 5 weeks after storage probably due to differences between initial and ripe kiwifruits, and SSC decreased with each passing week due to natural starch conversion over time. The SSC/acid ratio increased from 18 to 27 until 5 weeks after storage and then slowly declined in all kiwifruit stored at different low temperatures. Sensory evaluation results showed no differences in kiwifruit flesh color stored at two storage temperatures of $2^{\circ}C$ and $6^{\circ}C$.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.4
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• pp.473-479
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• 2018

Objective: The study was designed to perform a genome-wide association (GWA) and partitioning of genome using Illumina's PorcineSNP60 Beadchip in order to identify variants and determine the explained heritability for the total number of teats in Yorkshire pig. Methods: After screening with the following criteria: minor allele frequency, $MAF{\leq}0.01$; Hardy-Weinberg equilibrium, $HWE{\leq}0.000001$, a pair-wise genomic relationship matrix was produced using 42,953 single nucleotide polymorphisms (SNPs). A genome-wide mixed linear model-based association analysis (MLMA) was conducted. And for estimating the explained heritability with genome- or chromosome-wide SNPs the genetic relatedness estimation through maximum likelihood approach was used in our study. Results: The MLMA analysis and false discovery rate p-values identified three significant SNPs on two different chromosomes (rs81476910 and rs81405825 on SSC8; rs81332615 on SSC13) for total number of teats. Besides, we estimated that 30% of variance could be explained by all of the common SNPs on the autosomal chromosomes for the trait. The maximum amount of heritability obtained by partitioning the genome were $0.22{\pm}0.05$, $0.16{\pm}0.05$, $0.10{\pm}0.03$ and $0.08{\pm}0.03$ on SSC7, SSC13, SSC1, and SSC8, respectively. Of them, SSC7 explained the amount of estimated heritability along with a SNP (rs80805264) identified by genome-wide association studies at the empirical p value significance level of 2.35E-05 in our study. Interestingly, rs80805264 was found in a nearby quantitative trait loci (QTL) on SSC7 for the teat number trait as identified in a recent study. Moreover, all other significant SNPs were found within and/or close to some QTLs related to ovary weight, total number of born alive and age at puberty in pigs. Conclusion: The SNPs we identified unquestionably represent some of the important QTL regions as well as genes of interest in the genome for various physiological functions responsible for reproduction in pigs.

• Development and Reproduction
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• v.13 no.4
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• pp.305-312
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• 2009

Multipotent spermatogonial stem cells (mSSCs), derived from uni-potent SSC, are a type of reprogrammed cells with similar characteristics to embryonic stem cells (ESCs). The aim of this study was to evaluate the potential for transgenesis of mSSC derived from outbred mice and the production of transgenic animal by the mSSC-insertion into embryo. mSSCs, established from outbred mice (ICR strain) in the previous study, were maintained and then transfected with a lenti-viral vector expressing green fluorescent protein (GFP), CS-CDF-CG-PRE. Embryonic stem cells (ESCs) were derived from inbred transgenic mice (C57BL/6-Tg (CAG-EGFP)) and were used as an experimental control. Transfected mSSCs were well proliferated in vitro and maintained their characteristics and normal karyotype. Ten to twelve mSSCs and ESCs were collected and inserted into perivitelline space of 8-cell mouse embryos, and then transferred them into uteri of poster mothers after an additional 2-days of culture. Percentage of mSSC-derived offsprings was 4.8% (47/980) and which was lower than those (11.7% (67/572)) of ESC-derived ones (P<0.05). However, even though different genetic background of mSSC and ESC origin, the production efficiency of coat-colored chimeric offspring in mSSC group was not different when compared it with ESC (6.4% (3/47) vs. 7.5% (5/67)). From these results, we confirmed that mSSC derived from outbred mice has a pluripotency and a potential to produce chimeric embryos or mice when reaggregatation with mSSC is performed.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11b
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• pp.210-218
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• 2000

Since the SSC(soluble solid contents) and titratable acidity of fruit are highly concerned to the taste, the need for measuring them by non-destructive technology such as NIR(Visual and Near-infrared) spectroscopy is increasing. Specially, in order to grade the quality of each fruit with a sorter at sorting and packing facilities, technologies for online measurement satisfying the tolerance in terms of accuracy and speed should be developed. Many researches have been done to develop devices to measure the internal qualities of fruit such as SSC, titratable acidity, firmness, etc. with the VIS(Visual)/NIR(Near Infrared) reflectance spectra. The distributions of the SSC, titratable acidity, firmness, etc. are different with respect to the position and depth of fruit, and generally the VIS/NIR light can interact with fruit in a few millimeters of pathlength, and it is very difficult to measure the qualities of inner flesh of fruit. Therefore, to measure the average concentrations of each quality factor such as SSC and titratable acidity with the reflectance-type NIR devices, the spectra of fruit at several positions should be measured. Recently, the interest about the transmittance-type VIS/NIR devices is increasing. NIR light can penetrate through the fruit about 1/10-1/1,000,000 %. Therefore, very intensive light source and very sensitive sensor should be adopted to measure the transmitted light spectra of intact fruit. The ultimate purpose of this study was to develop a device to measure the transmitted light spectra of intact fruit such as apple, pear, peach, etc. With the transmittance-type VIS/NIR device, the feasibility of measurement of the SSC and titratable acidity in intact fruit cultivated in Korea was tested. The results are summarized as follows; A simple measurement device which can measure the transmitted light spectra of intact fruit was constructed with sample holder, two 500W-tungsten halogen lamps, a real-time spectrometer having a very sensitive CCD array sensor and optical fiber probe. With the device, it was possible to measure the transmitted light spectra of intact fruit such as apple, pear and peach. Main factors affecting the intensity of transmitted light spectra were the size of sample, the radiation intensity of light source and the integration time of the detector. Sample holder should be designed so that direct light leakage to the probe could be protected. Preprocessing method to the raw spectrum data significantly influenced the performance of the nondestructive measurement of SSC and titratable acidity of intact fruit. Representative results of PLS models in predicting the SSC of peach were SEP of 0.558 Brix% and R2 of 0.819, and those in predicting titratable acidity were SEP of 0.056% and R2 of 0.655.

• Journal of Korea Water Resources Association
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• v.55 no.1
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• pp.43-57
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• 2022

Advances in measurement techniques have reduced measurement costs and enhanced safety resulting in less uncertainty. For example, an acoustic doppler current profiler (ADCP) based suspended sediment concentration (SSC) measurement technique is being accepted as an alternative to the conventional data collection method. In Korean rivers, horizontal ADCPs (H-ADCPs) are mounted on the automatic discharge monitoring stations, where SSC can be measured using the backscatter of ADCPs. However, automatic discharge monitoring stations and sediment monitoring stations do not always coincide which hinders the application of the new techniques that are not feasible to some stations. This work presents and analyzes H-ADCP-SSC models for 9 discharge monitoring stations in Korean rivers. In application of the Gaussian mixture model (GMM) to sediment-related variables (catchment area, particle size distributions of suspended sediment and bed material, water discharge-sediment discharge curves) from 44 sediment monitoring stations, it is revealed that those characteristics can distinguish sediment monitoring stations regionally. Linking the two results, we propose a protocol determining the H-ADCP-SSC model where no H-ADCP-SSC model is available.

• Journal of Bio-Environment Control
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• v.29 no.4
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• pp.406-413
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• 2020

This study was conducted to find out optimum fruiting node order, pinching node order, and harvesting time in hydroponics using coir substrates to produce high quality melon (Cucumis melo L.) fruit. Three plants per coir slab (100 × 20 × 10 cm) were planted for each treatment. Yamazaki standard nutrient solutions for melon were supplied with 1.8, 2.0, and 2.3 dS·m^-1 at the early, middle (fruit enlargement step), and late growth stages, respectively. Two cultivars of 'PMR Dalgona' and 'Earl's Aibi' were used for fruiting node order and pinching node order experiments. Fruiting node treatments were conducted three replications (8-10 ^th, 11-13 ^th, and 14-15 ^th nodes) and pinching node treatments treated with three replications (18 ^th, 21 ^th, and 24 ^th nodes). Two cultivars of 'PMR Dalgona' and 'Earl's Crown' were used for fruit harvesting time experiment and treated with in four replications (45, 50, 55, and 60 days after fruiting). In growth characteristics, the leaf width and leaf area of 'PMR Dalgona' were the greatest 28.2 cm and 10,845 ㎠. Respectively, 11-13 ^th fruiting nodes or more. The node length of 'Earl's Aibi' was the longest by 147.6 cm at 11-13 ^th fruiting nodes. For fruit quality characteristics, the fruit weight of 'Earl's Aibi' at 11-13 ^th fruiting node fruiting was the greatest by 2.0 kg. The soluble solids content (SSC) of 'PMR Dalgona' was the highest by 14.5 °Brix at 8-10 ^th nodes in fruiting node orders and 14.5 °Brix at the 24 ^th pinching node order, respectively with significant difference. The SSC tends to increase in the same for both cultivars of 'PMR Dalgona' and 'Earl's Aibi' as the position of fruiting node was lower. The SSC and fruit weight of melon harvested at 55-60 days after fruiting was the best. From the results of this study, most of SSC tends to increase in the lower position of fruiting node order and the higher pinching node order, whereas the fruit weight shows a tendency of increasing with higher fruiting node. In addition, the SSC of fruit increased as the number of days after fruiting increased, and further research is needed for more various cultivars. In melon hydroponics using coir substrates, it is needed to figure out the characteristics of each cultivar to determine optimum fruiting node order, pinching node order, and fruit harvest time.

• Proceedings of the Korean Nuclear Society Conference
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• 2002.05a
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• pp.197.2-197
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• 2002

• Informatization Policy
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• v.21 no.3
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• pp.33-55
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• 2014

Public Shared Service Centers (PSSC) consolidate functions such as human resources, information technology, financial management or accounting into one office to serve an organization. A prominent example from the United States is the Federal Information Technology Shared Services Strategy. It supports mission, administrative, and infrastructure-related IT functions through providing organizations in the Executive Branch of the Federal Government (Federal Agencies) with policy guidance on the full range and lifecycle of intra- and inter-agency information technology (IT) shared services. This study looks at the Federal Information Technology Shared Services Strategy in order to draw lessons for fostering collaborative government through the use of PSSC. It finds the following factors are critical for success when implementing IT shared services: (1) agency leadership must be solidly behind their IT shared service plan, or the needed changes will not happen at the business unit, program, or system levels; (2) there must be a move away from internally-centered, program-specific thinking, and a move toward a paradigm of consuming and providing IT shared services with multiple groups whenever possible; and (3) successfully managing "loss of control" issues is central, and optimizing business processes is essential to move from stove-piped workflows to processes that work across the agency enterprise and beyond. Therefore, the study suggests that a Korean model of PSSC implementation be introduced, and that good IT Governance is a crucial component of PSSC strategies.