• Title/Summary/Keyword: SRA

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Identification and functional prediction of long non-coding RNAs related to oxidative stress in the jejunum of piglets

  • Jinbao Li;Jianmin Zhang;Xinlin Jin;Shiyin Li;Yingbin Du;Yongqing Zeng;Jin Wang;Wei Chen
    • Animal Bioscience
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    • v.37 no.2
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    • pp.193-202
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    • 2024
  • Objective: Oxidative stress (OS) is a pathological process arising from the excessive production of free radicals in the body. It has the potential to alter animal gene expression and cause damage to the jejunum. However, there have been few reports of changes in the expression of long noncoding RNAs (lncRNAs) in the jejunum in piglets under OS. The purpose of this research was to examine how lncRNAs in piglet jejunum change under OS. Methods: The abdominal cavities of piglets were injected with diquat (DQ) to produce OS. Raw reads were downloaded from the SRA database. RNA-seq was utilized to study the expression of lncRNAs in piglets under OS. Additionally, six randomly selected lncRNAs were verified using quantitative real-time polymerase chain reaction (qRT-PCR) to examine the mechanism of oxidative damage. Results: A total of 79 lncRNAs were differentially expressed (DE) in the treatment group compared to the negative control group. The target genes of DE lncRNAs were enriched in gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes (KEGG) signaling pathways. Chemical carcinogenesis-reactive oxygen species, the Foxo signaling pathway, colorectal cancer, and the AMPK signaling pathway were all linked to OS. Conclusion: Our results demonstrated that DQ-induced OS causes differential expression of lncRNAs, laying the groundwork for future research into the processes involved in the jejunum's response to OS.

Tolerance of Rice, Soybean, and Hot Pepper to Simulated Acid Rain at Different Growth Stages (벼, 콩, 고추의 생육시간별 인공 산성비에 대한 내성)

  • 이석순;김태주;김복진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.6
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    • pp.548-555
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    • 1994
  • To compare the tolerance of crops to acid rain at different growth stages a simulated acid rain (SAR) of pH 2.7 was applied to rice, soybean, and hot pepper from vegetative growth stage to harvest (Veget.-Harvest) and from reproductive growth stage to harvest (Reprod.-Harvest). Visual damages of crops by SAR were greater in the order of rice < hot pepper < soybean and greater at Veget.-Harvest than at Reprod.-Harvest treatment. Chlorophyll content of all crops was greater in the order of Veget.-Harvest < Reprod.-Harvest treatment < control, but photosynthetic activity was not affected by SAR treatments. Nitrogen concentration and uptake of rice plants at harvest were similar among SAR treatments, but those of soybean and hot pepper were greater at Veget.-Harvest treatment than at Reprod.-Harvest treatment or control. Sulfur concentration of all crops was not affected by SAR treatments, but total sulfur uptake of soybean was greater in SAR treatments than untreated control. Grain yield of rice and soybean was not affected by SAR although grain fertility, percent ripened grains, and 1,000-grain weight of rice at Veget.-Harvest treatment were lower compared with Reprod.-Harvest treatment or control. Fruit dry weight of hot pepper was greater in the order of Veget.-Harvest < Reprod.-Harvest < control due to decreased fruit number per plant and average fruit weight. At one time application of SAR at flowering stage, brown spots were observed on the spikelets of rice at below pH 2.3. Petals of soybean and hot pepper were wilted at pH below 1. 7 and 2.0, respectively, but fruit setting was not affected by the pH of the SRA.

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The Situation and the Tasks of UK Rail Privatization, Focusing on after the Hatfield Accident (영국 철도 민영화의 현황 및 과제 (Hatfield사고 이후의 변화를 중심으로))

  • Lee, Yong-Sang
    • Journal of Korean Society of Transportation
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    • v.24 no.2 s.88
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    • pp.91-100
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    • 2006
  • This paper examines the situation and tasks of UK rail privatization, especially focusing on after the Hatfield rail accident. Earlier research which focused on the UK's Privatization had little knowledge of the explanations for recent changes. Moreover they had difficulty making a direct comparison between national rail and the privatized rail. Therefore we aye left without a good explanation which has a comprehensive perspective. I attempt to show the change in the rail privatization Process and its outcome, focusing on after the Hatfield rail accident. This Paper argues that the UK's vail privatization process has a regulatory framework which is too complicated with overlapping responsibilities that brought about inefficiency, increasing costs and a superficial safety regime. Especially the planning of rail and infrastructure maintenance did not come to play an appropriate role. However after 2000, the government took charge of setting the strategy for railways, and the Office of Rail Regulation covered safety performance and cost. explain that these changes present a good opportunity to solve the problem of passing the buck for poor performance. Through the analysis, I find that the passenger rail network is well-suited to deliver long distance business and commuters and that the subsidy from the government is decreasing. However, performance, for example punctuality and reliability. should be improved. Especially the Hatfield rail accident caused a reduction in the satisfaction of passengers. In future. the problems of rising costs and monopoly franchise system should be addressed.

A study on the level of codependency among social work related professionals and its effect on their burnout: Focusing on moderating effects of Sex-role attitude (사회복지종사자의 공동의존성과 소진에 관한 연구 : 성역할태도의 조절효과를 중심으로)

  • Jang, Gyeongho;Yoon, Kyeonga;Shim, Woochan
    • Korean Journal of Social Welfare Studies
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    • v.47 no.1
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    • pp.29-56
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    • 2016
  • The purpose of this study is first, to explore the level of codependency among social work related professionals since codependency of professionals in the field has not been explored much in Korea. Second purpose is to examine whether codependency affects the level of burnout. Last purpose is to examine moderating effects of the Sex-role attitude on the relationship between codependency and burnout. Data were collected from the 11th of December in 2014 until the 28th of February in the following year. Self-administered questionnaires were distributed to care professionals practicing in social work institutions in Daejeon City and Choongcheong province, via email and mail. In addition, surveys were administered at several continuing education training venues. Instruments used for this study include CODAT(Codependency Assessment Tool) developed by Hughes-Hammer and colleagues in 1998, MBI(Maslach Burnout Inventory) developed by Maslach and Jackson in 1981, and SRA(Sex Role Attitude) developed by Osmond and Maryin in 1975. In total, 368 surveys were used for final analysis. The results include that gender did not have statistically significant relationship with the overall codependency, however, male showed statistically higher score than female in one of subscales of codependency called hiding oneself. Workers in the residential settings showed higher level of codependency than those in non-residential settings, especially in the subscale of Other focus or Self-neglect. Codependency showed a strong relationship with Burnout. Among the five-factor structure of the CODAT, Medical problem, Low Self-worth and Family of Origin showed strong relationship with the Burnout score. And the moderating effects of Sex-role Attitude showed a statistically significant relationship between codependency and burnout. Conservative sex-role attitude amplified the level of burnout on the relationship between codependency and burnout. Based on the result, educational and policy implications were made in order to prevent burnout among social work related professionals.