• Korean Journal of Veterinary Service
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• v.34 no.1
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• pp.1-4
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• 2011

A surface plasmon resonance imaging (SPRI) assay was developed for measuring porcine circovirus type 2 (PCV2) antibody using a recombinant capsid protein as an antigen. The diagnostic potential of SPRI for detecting antibodies to the PCV2 capsid protein was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 70 pig serum samples taken from 6 pig farms. There was a strong positive correlation between the SPRI and ELISA (n = 70, r = 0.911, P<0.01). Therefore, this recombinant capsid protein can be used as an antigen for serological studies, and the SPRI, a label-free and high-throughput method, is expected to be a valuable tool in the serodiagnosis of PCV2 infection.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.4 s.48
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• pp.479-483
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• 2004

An attempt was made to develop a proteinchip for screening of MITF (microphthalmia transcription factor) inhibitor. Binding of MITF to E-box causes transcription of several pigmenting genes including tyrosinase gene. We investigated binding of MITF and its DNA binding site (E-box) using a protein-DNA chip with various detection methods including flurorescence (Cyt3). SPR (surface plasmon resonance) and SPRi (surface plasmon resonance imaging). A fusion protein (MITF-Maltose Binding Protein) was attached on the glass plate by chemical modification. An inhibitory synthetic DNA oligomer, artificially designed based on the E-box sequence, inhibited the binding of MITF and E-box. These results showed the potentials of flurorescence-based MITF protein chip as a microarray for high throughput screening (HTS) system of depigmenting agents.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.3
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• pp.531-537
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• 2007

This paper presents a new method for determining L, Cs, and Cp in a series parallel resonant inverter. This inverter is suitable for driving high-intensity discharge (HID) lamps. The operating frequency is selected to avoid acoustic resonances which are governed by the size and shape of the arc tube. L, Cs and Cp, are chosen to satisfy the ignition and steady state requirements of the lamp. Equations are developed to analyze the starting condition and steady state operating condition of the lamp. For the prototype ballast for a 250W Metal Halide Lamp, experimental results are presented in order to validate the proposed method.

• Journal of Ginseng Research
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• v.46 no.1
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• pp.156-166
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• 2022

Background: Panax ginseng Meyer (P. ginseng), a herb distributed in Korea, China and Japan, exerts benefits on diverse inflammatory conditions. However, the underlying mechanism and active ingredients remains largely unclear. Herein, we aimed to explore the active ingredients of P. ginseng against inflammation and elucidate underlying mechanisms. Methods: Inflammation model was constructed by lipopolysaccharide (LPS) in C57BL/6 mice and RAW264.7 macrophages. Molecular docking, molecular dynamics, surface plasmon resonance imaging (SPRi) and immunofluorescence were utilized to predict active component. Results: P. ginseng significantly inhibited LPS-induced lung injury and the expression of proinflammatory factors, including TNF-α, IL-6 and IL-1β. Additionally, P. ginseng blocked fluorescencelabeled LPS (LPS488) binding to the membranes of RAW264.7 macrophages, the phosphorylation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs). Furthermore, molecular docking demonstrated that ginsenoside Ro (GRo) docked into the LPS binding site of toll like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD2) complex. Molecular dynamic simulations showed that the MD2-GRo binding conformation was stable. SPRi demonstrated an excellent interaction between TLR4/ MD2 complex and GRo (K_D value of 1.16 × 10^-9 M). GRo significantly inhibited LPS488 binding to cell membranes. Further studies showed that GRo markedly suppressed LPS-triggered lung injury, the transcription and secretion levels of TNF-α, IL-6 and IL-1β. Moreover, the phosphorylation of NF-κB and MAPKs as well as the p65 subunit nuclear translocation were inhibited by GRo dose-dependently. Conclusion: Our results suggest that GRo exerts anti-inflammation actions by direct inhibition of TLR4 signaling pathway.

• Journal of Ginseng Research
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• v.47 no.6
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• pp.743-754
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• 2023

Background: Myocardial fibrosis post-myocardial infarction (MI) can induce maladaptive cardiac remodeling as well as heart failure. Although 20(S)-ginsenoside Rg3 (Rg3) has been applied to cardiovascular diseases, its efficacy and specific molecular mechanism in myocardial fibrosis are largely unknown. Herein, we aimed to explore whether TGFBR1 signaling was involved in Rg3's anti-fibrotic effect post-MI. Methods: Left anterior descending (LAD) coronary artery ligation-induced MI mice and TGF-β1-stimulated primary cardiac fibroblasts (CFs) were adopted. Echocardiography, hematoxlin-eosin and Masson staining, Western-blot and immunohistochemistry, CCK8 and Edu were used to study the effects of Rg3 on myocardial fibrosis and TGFBR1 signaling. The combination mechanism of Rg3 and TGFBR1 was explored by surface plasmon resonance imaging (SPRi). Moreover, myocardial Tgfbr1-deficient mice and TGFBR1 adenovirus were adopted to confirm the pharmacological mechanism of Rg3. Results: In vivo experiments, Rg3 ameliorated myocardial fibrosis and hypertrophy and enhanced cardiac function. Rg3-TGFBR1 had the 1.78×10^-7 M equilibrium dissociation constant based on SPRi analysis, and Rg3 inhibited the activation of TGFBR1/Smads signaling dose-dependently. Cardiac-specific Tgfbr1 knockdown abolished Rg3's protection against myocardial fibrosis post-MI. In addition, Rg3 downregulated the TGF-β1-mediated CFs growth together with collagen production in vitro through TGFBR1 signaling. Moreover, TGFBR1 adenovirus partially blocked the inhibitory effect of Rg3. Conclusion: Rg3 improves myocardial fibrosis and cardiac function through suppressing CFs proliferation along with collagen deposition by inactivation of TGFBR1 pathway.

• Journal of Korea Multimedia Society
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• v.12 no.8
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• pp.1182-1189
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• 2009

A potential advantage of computer-based education is individualized instruction. In this regard, issues regarding how to evaluate individual learners fast and accurately in order to implement customized instruction program and to open education are raised. In this study, we suggest through a simulation study a way to reach an optimal decision by complementing the insufficient point of the way of the decision of the existing SPRT(Sequential Probability Ratio Testing) in which the number of questions in a test decreases while decision on pass or fail is relatively exact. For the purpose, the data of the examinees obtained from actual tests were used in this study and we present that simulation to verify the accuracy of decision and Multilevel SPRT decision standard for initial set point for optimal SPRT decision and evaluate the ground of the same through simulation.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.2
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• pp.143-146
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• 2004

A surface plasmon resonance (SPR) imaging system was constructed and used to detect the hexahistidine-ubiquitin-tagged human parathyroid hormone fragment (His$\sub$6/-Ub-hPTHF(1-34)) expressed in Escherichia coli. The hexahistidine-specific antibody was immobilized on a thin gold film coated with ProLinker$\^$TM/ B, a novel calixcrown derivative with a bifunctional coupling property that permits efficient immobilizaton of capture proteins on solid matrices. The soluble and insoluble fractions of an E. coli cell lysate were spotted onto the antibody-coated gold chip, which was then washed with buffer (pH 7.4) solution and dried. SPR imaging measurements were carried out to detect the expressed His$\sub$6/-Ub-hPTHF(1-34). There was no discernible protein image in the uninduced cell lysate, indicating that non-specific binding of contaminant proteins did not occur on the gold chip surface. It is expected that the approach used here to detect affinity-tagged recombinant proteins using an SPR imaging technique could be used as a powerful tool for the analyses of a number of proteins in a high-throughput mode.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.26 no.4
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• pp.1037-1055
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• 2016

After the enactment of the Personal Information Protection Act, policies and activities for the personal information protection have been actively promoted. However the people are showing negative attitudes about personal information, as the ongoing personal data leakages. Therefore, authors tried to empirical analysis of the effectiveness of national policy on the protection of personal information, using SERVQUAL model, focused on the people's perception, in order to identify that how the people recognized current policy. Authors find that the public has perceived the effectiveness of the policy positively, but the level of their awareness is low. And we identify that the people are highly aware of the policy's effectiveness for Immediacy, Convenience and Responsibility, while they have the lowest effectiveness for Efficiency. The policy's improvement focused on the public's low expectations/perceptions and effectiveness awareness, is required in order to develop people-oriented national privacy policy that are satisfied by the people.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.24 no.6
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• pp.1243-1261
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• 2014

With the methods of electronic financial frauds becoming advanced, economic losses have greatly increased. The Electronic Financial Fraud Prevention Service(hereafter EFFPS) has taken effect to prevent electronic financial frauds, but economic losses still occurring. This paper aimed to suggest a direction for improvement of the EFFPS, through the analysis of electronic financial fraud cases. As a result of analysis on the fraud cases before and after implementation of the EFFPS, 'Fraud using Smartphone App' and 'Fraud using Calls and SMS' were increased after implementation of the EFFPS, and also the damage cost of 'Fraud using Smartphone App' had increased. Also we revealed some limitations of the EFFPS. For complementing this limitations, authors considered direction for improvement of the EFFPS focus on application of current services/systems related prevention of electronic financial fraud and considered the ways that are make connection with several measurements related prevention currently being discussed and implemented in perspective of defense in depth.

• The Korean Journal of Food And Nutrition
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• v.13 no.6
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• pp.570-577
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• 2000

Korean duok has a many kinds of shape and cooking preparation and they were improved variably Also they were famous for special festival food and theirs demand are becoming popular. Since theirs retrogradation are degraded its taste and texture during the storage which should be resolved in most food industry. There is a possibility that storage temperaure and additives may involve in retrogradation. Recently Shon et al. reported that some sugar alcohols was effective for reducing it. We can search a kinds of additives, trehalose which can protect from shocks by freezing or heating. Furthermore they emphasized the trehalose can act as substitute for bound water in dehydration situation, and trehalose more effective than oligosaccharide or sugar alcohol. Therefore we tried to exam the changes of many characteristics of glutinous and rice duck with 2 levels trehalose in the storage.