• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.2
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• pp.154-162
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• 2006

Cheese analogs using lipoxygenase-defected soymilk and ${\alpha}$-chymotrypsin modified soy protein isolate (SPI) were prepared. Color, textural properties, sensory attributes and melting spreadability of cheese analogs were evaluated and compared with mozzarella cheese, and relationships between textural properties, sensory attributes and melting spreadability of cheese analogs were analyzed. Off-flavors were not mostly discriminated. Cheese analogs containing 10% SPI untreated and containing 6% and 8% SPI treated by ${\alpha}$-chymotrypsin in ${\Delta}E$ value of color were the most similar to mozzarella cheese. Quality characteristics and melting spreadability of cheese analogs were highly affected and improved by ${\alpha}$-chymotrypsin modification. Sensory attributes and melting spreadability of cheese analogs containing 6% SPI treated by ${\alpha}$-chymotrypsin were the most similar to mozzarella cheese, while in textural properties, cheese analogs containing 10% SPI were the most similar with mozzarella cheese. Hardness in sensory attributes was highly positively correlated with hardness (r>0.65), adhesiveness (r>0.56), chewiness (r>0.77) and gumminess (r>0.76) in textural properties, while it was highly negatively correlated with melting spreadability (r>-0.68).

• Preventive Nutrition and Food Science
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• v.8 no.3
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• pp.278-283
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• 2003

Textural properties of tofu manufactured with micronized full-fat soyflour (MFS) were enhanced by the addition of soy protein isolate, whey protein concentrate, chitosan oligosaccharide and mushroom powder. The MFS solution (14.2% solid content) was converted to semi-solid tofu by a two-stage heat treatment with the addition of 4% coagulant mix. The MFS tofu was evaluated by a compression test as well as sensory evaluation. To produce the semi-solid gel (MFS tofu) with reasonably high strength and toughness, the MFS solution with 14.2% solid content and 7.0% protein had to be heat treated at 121$^{\circ}C$ for 3min. The relative toughness of MFS tofu was increased by the addition of SPI, showing a 144% increase. The toughness of MFS tofu prepared with the MFS/SPI mixture was greatly increased by the addition of WPC at the level of 0.7% and the water separation from MFS tofu was greatly reduced. Furthermore, the toughness and strength of MFS/SPI tofu was enhanced by the addition of 0.1% chitosan oligosaccharide and 0.2% mushroom powder. The sensory evaluation of the tofu fortified with SPI, chitosan oligosaccharide and mushroom powder was superior to that of MFS tofu, with a higher score for overall preference.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.349-349
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• 2017

표준강수지수(Standardized precipitation index, SPI)는 가장 널리 사용되고 있는 가뭄지수로, 우리나라 뿐만 아니라 세계기상기구(World Meteorological Organization)에서도 추천하고 있는 대표적인 기상학적 가뭄 지수라고 할 수 있다. 현재 표준강수지수는 2변수 gamma 분포를 적용하여 강수 부족 상황을 지수화하여 나타내고 있는데, 일부 연구에서는 다른 확률분포형의 적용하기도 하였다(Guttman, 1999; Lloyd-Hughes and Saunders, 2002; Stagge et al., 2015). 우리나라에서는 유원희(2000)에 의해 Pearson type 3, 2변수 gamma, generalized logistic, GEV, 3변수 log-normal 분포에 따른 SPI 산정 결과를 비교한 연구가 수행되었는데, SPI 산정에는 분포형별 차이가 뚜렷하지 않다는 결론을 얻었다. 그러나 이때 금강유역 내 지점에 국한하여 적용하였고, 분포형별 적합도 검정을 수행하지 않고 SPI 산정결과만을 비교하여 우리나라에 일반적으로 적용하기에는 어려움이 있다. 따라서 본 연구에서는 우리나라의 강우특성을 반영할 수 있도록 다양한 확률분포형을 고려하여 표준강수지수를 분석하고자 한다. 이를 위해 관측기간이 30년 이상인 기상관측소의 월단위 강우자료를 구축하고, 월단위 강우자료에 다양한 확률분포형을 적용하고자 한다. 이때 적용하는 확률분포형은 2변수 gamma, Gumbel, normal 분포이다. 적정 확률분포형 선정을 위해 적합도 검정을 수행하고자 한다. 또한 각 분포형별로 산정된 표준강수지수를 기존 표준강수지수와 비교검토하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.8
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• pp.1135-1142
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• 2017

Objective: The objective of this study was to evaluate effects of heat treatment and soybean oil inclusion on protein oxidation of soy protein isolate (SPI) and of oxidized protein on redox status of broilers at an early age. Methods: SPI mixed with soybean oil (SPIO) heated at $100^{\circ}C$ for 8 h was used to evaluate protein oxidation of SPI. A total of two hundred and sixteen 1-day-old Arbor Acres chicks were divided into 3 groups with 6 replicates of 12 birds, receiving basal diet (CON), heat-oxidized SPI diet (HSPI) or mixture of SPI and 2% soybean oil diet (HSPIO) for 21 d, respectively. Results: Increased protein carbonyl, decreased protein sulfhydryl of SPI were observed as heating time increased in all treatments (p<0.05). Addition of 2% soybean oil increased protein carbonyl of SPI at 8 h heating (p<0.05). Dietary HSPI and HSPIO decreased the average daily gain of broilers as compared with the CON (p<0.05). Broilers fed HSPI and HSPIO exhibited decreased glutathione (GSH) in serum, catalase activity and total sulfhydryl in liver and increased malondialdehyde (MDA) and protein carbonyl in serum, advanced oxidation protein products (AOPPs) in liver and protein carbonyl in jejunal mucosa as compared with that of the CON (p<0.05). Additionally, broilers receiving HSPIO showed decreased glutathione peroxidase activity (GSH-Px) in serum, GSH and hydroxyl radical scavenging capacity in liver, GSH-Px activity in duodenal mucosa, GSH-Px activity and superoxide anion radical scavenging capacity in jejunal mucosa and increased AOPPs in serum, MDA and protein carbonyl in liver, MDA and AOPPs in jejunal mucosa (p<0.05). Conclusion: Protein oxidation of SPI can be induced by heat and soybean oil and oxidized protein resulted in redox imbalance in broilers at an early age.

• Journal of Korea Water Resources Association
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• v.47 no.2
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• pp.171-182
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• 2014

The objective of this study is to evaluate the utilization of bivariate joint drought index in South Korea. In order to develop the bivariate joint drought index, in this study, Clayton copula was used to estimate the joint distribution function and the calibration method was employed for parameter estimation. Precipitation and soil moisture data were selected as input data of bivariate joint drought index for period of 1977~2012. The time series analysis, ROC (Receiver Operating Characteristic) analysis, spatial analysis were used to evaluate the bivariate joint drought index with SPI (Standardized Precipitation Index) and SSI (Standardized Soil moisture Index). As a result, SPI performed better for drought onset and SSI for drought demise. On the other hand the bivariate joint drought index captured both drought onset and demise very well. The ROC score of bivariate joint drought index was higher than that of SPI and SSI, and it also reflected the local drought situations. The bivariate joint drought index overcomes the limitations of existing drought indices and is useful for drought analysis.

• Applied Biological Chemistry
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• v.46 no.2
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• pp.113-116
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• 2003

Color value, total color difference $({\triangle}E)$, puncture strength (PS), tensile strength (TS), percent elongation(%E) at break values, and water vapor permeability (WVP) were determined for water-soluble chitosan films added with soy protein isolate (SPI) and $CaCl_2$. Mechanical and water vapor barrier properties of the chitosan films were affected by SPI and $CaCl_2$ concentrations. PS value $(174.5-448.8\;g_f)$ of the chitosan films increased with increasing concentration of $CaCl_2$ TS value of 0.05% $CaCl_2$-added chitosan films significantly increased to 1.58 MPa compared to 0.06 MPa of the control. %E values ranged from 12.25 to 44.80% and increased with increasing concentration of $CaCl_2$. WVP of $CaCl_2$-added films was lower than that of SPI-added films.

• Korean Journal of Food Science and Technology
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• v.25 no.1
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• pp.39-45
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• 1993

The effects of enzymatic modification with pepsin and actinidin was studied on molecular weight distributions and functional properties of hydrolysates from soy protein isolate (SPI) differing in degree of hydrolysis. The hydrolyzed SPI by pepsin showed 41.5% degree of hydrolysis after 5 min, and maximum hydrolysis was obtained after 2 hours. Actinidin hydrolyzed SPI 26.71% degree after 1 hour. On SDS-PAGE, native SPI showed 9 distinguishable bands on SDS-PAGE gel. Pepsin treated SPI showed one broad band in the lower part of gel. This band was shifted further to the bottom of the gel and became faint as hydrolysis time increased. While actinidin treated SPI showed different SDS-PAGE pattern from pepsin. However PAGE patterns were similar with pepsin and actinidin treated groups. With pepsin treatment, solubility of SPI distinctively increased around isoelectric point(pI). Emulsifying activity (EA) and emulsifying stability (ES) showed marked increase over pH range of $3.0{\sim}8.0$. 5 min modified group had most excellent foam expansion (FE). Foam stability (FS) was increased as pepsin treatment time increased at pI. With actinidin treatment, solubility was increased. 60 min modified SPI had the most effective EA at pH 4.5. However ES was not effected by actinidin treatment. 5 min modified group was most effect in FE. FS was higher at alkaline pH.

• Korean Journal of Food Science and Technology
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• v.19 no.6
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• pp.499-505
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• 1987

The functional properties of lupin seed protein concentrate (LPC) were examined and compared to those of soybean protein isolate (SPI) and Na-caseinate. LPC-50, of which protein level was 50%, was prepared by a two phase solvent (hexane: alcohol: water= 10:7:3) extraction method. LPC-70 was made from LPC-50 by removing the fractions solubilized by carbohydrate decomposing enzymes. The solubilities of LPC-50 and LPC-70 were similar to that of of SPI but slightly higher at pH 4-5, and less susceptible to the added salt. The apparent viscosity of LPC increased exponentially as the concentration increased over 6% level, and the change was similar to that of Na-caseinate. LPC showed strong pseudoplastic non-Newtonean flow behavior, which was similar to that of SPI The emulsifying capasity of LPC-70 was similar to that of SPI when salt was added. The foaming capacity of LPC was comparable to that of SPI. LPC showed high oil and water absorption capacities, which increased as the protein level was elevated. LPC-70 showed the highest oil absorption capacity of all the samples tested.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.330-330
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• 2021

본 연구에서는 새롭게 개발 중인 SSP 시나리오의 일단위 강수량과 온도 자료를 활용하여 청미천 유역의 미래 가뭄의 예측 및 분석을 실시하였다. SSP1-2.6, SSP2-4.5, SSP3-7.0, SSP5-8.5에 따른 새롭게 개발 중인 CMIP6 (Coupled Model Intercomparison Project) GCM (General Circulation Models) 중 ACCESS-ESM1.5(Australian Community Climate and Earth System Simulator model)를 이용하였다. GCM 자료는 Quantile Mapping 방법을 사용하여 편이보정 되었고, 유출분석은 SWAT(Soil and Water Assessment Tool) 모형을 사용하여 청미천 유역에 대해 수행하였다. 청미천 유역의 가뭄분석을 위해 기상학적 가뭄지수인 SPI(Standardized Precipitation Index)와 SPEI(Standardized Precipitation Evapotranspiration Index), 수문학적 가뭄지수인 SDI(Standardized Streamflow Index)를 산정하였다. 그 후, 시간에 따른 가뭄의 특성을 분석하기 위해 가까운 미래 (2025-2064)와 먼 미래 (2065-2100) 로 구분하여 분석을 진행하였다. 그 결과, 청미천 유역의 가뭄 발생은 SSP시나리오, 가뭄지수에 따라 차이점을 확인할 수 있었다. SSP 시나리오의 경우 SSP5-8.5에서 가장 심각한 가뭄이 발생하였다. 가뭄지수의 경우 강수만을 고려한 SPI는 먼 미래에 비해 가까운 미래에서 더욱 심각한 가뭄이 발생하였다. SDI의 경우 강수량의 변동이 일반적으로 하천의 흐름에 영향을 미치기에 SPI와 비슷한 양상을 나타내었다. SPEI의 경우 시간에 따른 기온상승으로 먼 미래에 심각한 가뭄이 발생하였다.

• Journal of Microbiology and Biotechnology
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• v.23 no.12
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• pp.1664-1672
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• 2013

In Salmonella enterica serovar Typhimurium, many genes encoded within Salmonella pathogenicity island 1 (SPI1) are required to induce intestinal/diarrheal disease. In this study, we compared the expression of four SPI1 genes (hilA, invF, prgH, and sipC) under shaking and standing culture conditions and found that the expression of these genes was highest during the transition from the exponential to stationary phase under shaking conditions. To identify regulators associated with the stationary phase-dependent activation of SPI1, the effects of selected regulatory genes, including relA/spoT (ppGpp), luxS, ihfB, hfq, and arcA, on the expression of hilA and invF were compared under shaking conditions. Mutations in the hfq and arcA genes caused a reduction in hilA and invF expression (more than 2-fold) in the early stationary phase only, whereas the lack of ppGpp and IHF decreased hilA and invF gene expression during the entire stationary phase. We also found that hfq and arcA mutations caused a reduction of hilD expression upon entry into the stationary phase under shaking culture conditions. Taken together, these results suggest that Hfq and ArcA regulate the hilD promoter, causing an accumulation of HilD, which can trigger a stationary phase-dependent activation of SPI1 genes under shaking culture conditions.