• Journal of Microbiology
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• v.35 no.3
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• pp.213-221
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• 1997

The secretion of the alkaliphilic Bacillus sp. S-1 extracellular pullulanase involves translocation across the cytoplasmic membrane of the Gram-positive bacterial cell envelope. Translocation of the intracellular pullulanase PUL-I, was traced to elucidate the mechanism and pathway of protein secretion from an alkaliphilic Bacillus sp. S-1. Pullulanase could be slowly bue quantitatively released into the medium during growth of the cells in medium contianing proteinase K. The released pullulanase lacked the N-terminal domain. The N-terminus is the sole membrane anchor in the pullulanase protein and was not affected by proteases, confirming that it is not exposed on the cell surface. Processing of a 180,000M$\_$r/ pullulanase to a 140,000M$\_$r/ polypeptide has been demonstrated in cell extracts using antibodies raised against 140,000M$\_$r/ extracellular form. Processing of the 180,000 M$\_$r/ protein occured during the preparation of extracts in an alkaline pH condition. A modified rapid extraction procedure suggested that the processing event also occured in vivo. Processing apparently increased the activity of pullulanase. The western blotting analysis with mouse anti-serum against 140-kDa extracellular pullulanase PUL-E showed that PUL-I is processed into PUL-X via intermediate form of PUL-E. Possible explanationa for the translocation are discussed.

• ALGAE
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• v.35 no.4
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• pp.325-335
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• 2020

A new diatom species, Mayamaea vietnamica sp. nov., is described from Cát Tiên National Park in Vietnam. This species was discovered and described from soil samples. Algae from soil ecosystems in Vietnam are almost unknown. The new species is described on the basis of an integrated approach with molecular and morphological data, and comparison with similar species. In terms of molecular data, 18S rDNA (including V4 domain), and partial rbcL plastid genes show M. vietnamica sp. nov. is most closely related to M. terrestris N. Abarca and R. Jahn, and together they form a monophyletic group relative to other members of the genus. M. vietnamica sp. nov. differs from other species in the genus by the number of striae and areolae in 10 ㎛, number of areolae per stria, as well as shape and presence or absence of axial and central areas.

• Journal of Broadcast Engineering
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• v.18 no.3
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• pp.455-462
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• 2013

This paper introduces the Spatially Preprocessed Speech Distortion Weighted Multi-channel Wiener Filter (SP-SDW-MWF) for multi-microphone noise reduction and proposes a method to determine the speech distortion weights. The SP-SDW-MWF is known as a robust noise reduction algorithm against the error caused by the mismatch in microphones. The SP-SDW-MWF adopts weights which determine the amount of noise reduction at the expense of introducing speech distortion in the noise-suppressed speech. In this paper, we use the error of power spectral density between the estimated signal and the desired signal as the evaluation measure. Thus the a priori SNR is used to control the speech distortion weights in the frequency domain. In the experimental results, the proposed method yields better result in terms of MFCC distortion compared to the conventional method.

• Nuclear Engineering and Technology
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• v.52 no.2
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• pp.230-237
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• 2020

In this part, an implicit time dependent solution is presented for the Boltzmann transport equation discretized by the analytic coarse mesh finite difference method (ACMFD) over the spatial domain as well as the simplified P₃ (SP₃) for the angular variable. In the first part of this work we proposed a SP₃-ACMFD approach to solve the static eigenvalue equations which provide the initial conditions for temp dependent equations. Having solved the 3D multi-group SP₃-ACMFD static equations, an implicit approach is resorted to ensure stability of time steps. An exponential behavior is assumed in transverse integrated equations to establish a relationship between flux moments and currents. Also, analytic integration is benefited for the time-dependent solution of precursor concentration equations. Finally, a multi-channel one-phase thermal hydraulic model is coupled to the proposed methodology. Transient equations are then solved at each step using the GMRES technique. To show the sufficiency of proposed transient SP₃-ACMFD approximation for a full core analysis, a comparison is made using transport peers as the reference. To further demonstrate superiority, results are compared with a 3D multi-group transient diffusion solver developed as a byproduct of this work. Outcomes confirm that the idea can be considered as an economic interim approach which is superior to the diffusion approximation, and comparable with transport in results.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.38 no.2
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• pp.227-236
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• 2001

This paper proposes a frequency-domain partially adaptive algorithm, called a censoring algorithm, to reduce the computational complexity of the frequency domain adaptive array. The proposed censoring algorithm determines the existence of interferences in the frequency-domain at each frequency bin using a constant false alarm rate (CFAR) processor. The censoring algorithm adapts only those parts of the weights that correspond to the frequency bins expected to contain interferences. The censoring algorithm is also expanded to overcome the signal cancellation phenomenon caused by smart jammers. Accordingly, a censoring spatial smoothing, which combines the censoring algorithm with spatial smoothing, is proposed. Simulation results show that the proposed algorithms are effective in removing interferences with only part of the computational complexity of conventional algorithms yet with the same level of performance.

• BMB Reports
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• v.41 no.2
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• pp.102-107
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• 2008

Extracellular proteases play an important role in a wide range of host physiological events, such as food digestion, extracellular matrix degradation, coagulation and immunity. Among the large extracellular protease family, serine proteases that contain a "paper clip"-like domain and are therefore referred to as CLIP-domain serine protease (clip-SP), have been found to be involved in unique biological processes, such as immunity and development. Despite the increasing amount of biochemical information available regarding the structure and function of clip-SPs, their in vivo physiological significance is not well known due to a lack of genetic studies. Recently, Drosophila has been shown to be a powerful genetic model system for the dissection of biological functions of the clip-SPs at the organism level. Here, the current knowledge regarding Drosophila clip-SPs has been summarized and future research directions to evaluate the role that clip-SPs play in Drosophila immunity are discussed.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.43 no.4 s.310
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• pp.108-115
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• 2006

This paper presents a watermarking algorithm in the discrete wavelet transform domain using evolutionary algorithm. The proposed algorithm consists of wavelet-domain watermark insertion and genetic algorithm-based watermark extraction. More specifically watermark is inserted to the low-frequency region of wavelet transform domain, and watermark extraction is efficiently performed by using the evolutionary algorithm. The proposed watermarking algorithm is robust against various attacks such as JPEG and JPEG2000 image compression and geometric transformations.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.48 no.1
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• pp.23-32
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• 2011

In this paper, we develop a method for generating thumbnail images at hybrid domain combined the spatial domain and transform domain. The proposed method generates a pixel of a thumbnail image by adding a DC value of residual transform coefficients and an average value of an estimate block. For effectively calculating average values of estimate blocks, we propose a method for reconstructing the boundary pixels of a block. In comparison to the conventional method of decoding the bit stream then scaling down the decoded images, the developed method reduces the complexity by more than 60% while producing identical thumbnail images.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.41 no.3
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• pp.25-34
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• 2004

The ability to perform the same operations in the transform domain as in the spatial domain is important for efficient image transmission through a channel. We perform image resizing, which includes magnifying and reducing the size, in the discrete cosine transform(DCT) domain and the effects of the transform domain approach are analyzed in the corresponding spatial domain. Based on this analysis, the two resizing algorithms are proposed. The first one further compresses the images encoded by the compression standard by reducing the size before compression, and the other reduces the loss of information while maintaining the conventional compression rate. Because of its compatibility with standard codec, these algorithms can be easily embedded in JPEG and MPEG codecs, which are widely used for the purpose of image storage and transmission. Experimental results show a reduction of about half the bit size with similar image quality and about a 2- or 3-dB quality improvements in the similar compression rate.

• Journal of Microbiology
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• v.43 no.1
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• pp.49-53
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• 2005

Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.