• Journal of Microbiology and Biotechnology
• /
• v.9 no.6
• /
• pp.804-810
• /
• 1999

There is a possibility that carvone, a monoterpene from spearmint (Mentha spicata), could induce the bph degradative pathway and genes in Alcaligenes eutrophus H850, which is a known Gram-negative PCB degrader with a broad substrate specificity that was thoroughly investigated with Arthrobacter sp. BIB, a Gram-positive PCB degrader. The strains BIB and H850 were unable to utilize and grow on the plant terpene [(R)-(-)-carvone] (50ppm) to be recognized as a sole carbon source. Nevertheless, the carvone did induce 2,3-dihydroxybiphenyl 1,2-dioxygenase (encoded by bphC) in the strain B lB, as observed by a resting cell assay that monitors accumulation of a yellow meta ring fission product from 4,4'-dichlorobiphenyl (DCBp). The monoterpene, however, did not appear to induce the meta cleavage pathway in the strain H850. Instead, an assumption was made that the strain might be using an alternative pathway, probably the ortho-cleavage pathway. A reverse transcription (RT)-PCR system, utilizing primers designed from a conserved region of the bphC gene of Arthrobacter sp. M5, was employed to verify the occurrence of the alternative pathway. A successful amplification (182bp) of mRNA transcribed from the N-terminal region of the bphC gene was accomplished in H850 cells induced by carvone (50ppm) as well as in biphenyl-growth cells. It is, therefore, likely that H850 possesses a specific PCB degradation pathway and hence a different substrate specificity compared with B1B. This study will contribute to an elucidation of the dynamic aspects of PCB bioremediation in terms of roles played by PCB degraders and plant terpenes as natural inducer substrates that are ubiquitous and environmentally compatible.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.50 no.4
• /
• pp.343-351
• /
• 2017

Sanitary evaluation of seawater and Pyropia sp. laver collected from the five major laver growing areas in Korea was performed four times over the course of a year. The seawater quality in four of these five areas was regarded as the clean area according to Korean criteria, but the seawater at one investigation site in Seoheon area was found to exceed the standard for fecal coliform. In the bacteriological safety analysis of laver (raw source), the percentages of samples not conforming to Chinese criteria at the five sites were 55.6% (Seocheon), 70.0% (Shinan), 81.8% [Jindo (Haenam)], 63.6% (Wando), and 28.6% [Goheung (Jangheung)]. Pathogenic bacteria were not detected in all laver samples. The food safety of laver (raw source) based on heavy metal concentration was confirmed using Korean criteria; the concentrations of heavy metals in laver samples collected from the major laver growing areas were 0.008-0.632 mg/kg wet weight (ww) lead, 0.024-0.137 mg/kg ww cadmium, 0.908-2.892 mg/kg ww total arsenic, and 0.003-0.013 mg/kg ww total mercury. Therefore, pollution source management and periodic monitoring of heavy metals may be required to improve the food safety of laver produced in these laver growing areas.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.14 no.5
• /
• pp.81-101
• /
• 2011

This study was conducted to understand the environmental and ecological function of habitat through evaluation of water environment, soil environment, vegetation characteristics, macro-invertebrate characteristics, and visual habits environment evaluation (SVAP) in Hongdong stream located in Hongseong-Gun, Chungnam Province, and hereafter to utilize the results for the habits reconstruction and improvement project. As the results of water quality analysis, BOD, COD, T-P was almost below the standard quality from upper stream to down stream. The construction of small reservoir, wetland and water purification facility, and the management of non-point pollution are proposed to improve these problems. The soil texture was sandy soil, which is unfitted with vegetation development. The construction of shallows and bogs, and induction of soil sedimentation and biotope formation are proposed to improve these problems. In the plant flora, total 90 kinds were observed with 81 species and nine varieties in total 36 families and 85 genera, and the naturalization rate was higher at down area than upper area. As the results of macro-invertebrate fauna survey, total 26 species and 297 individuals in 20 families and 22 genera were collected. Peltodytes sinensis, Chironomidae sp., and Culicidae sp., which are observed at polluted environment, were collected as dominant species. An appropriate vegetation management party idea is necessary, and it is done an idea in consideration of the soil and a physical characteristic. Visual habits environment evaluation (SVAP) result was mostly determined with below normal (Fair) grade. Pollution source interception, purification pond establishment, and various bog establishment are proposed to improve these problems. With the above results, the ecological environment was determined with bad condition, and the improvement of biotope was urgently needed through sustainable monitoring and management of streams habitat in rural area.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.3
• /
• pp.267-273
• /
• 2011

Methyl violet, used extensively in the commercial textile industry and as a biological stain, is a hazardous recalcitrant. Aspergillus sp. strain CB-TKL-1 isolated from a water sample from Tsumoriri Lake, Karzok, Ladakh, India, was found to completely decolorize methyl violet within 24 h when cultured under aerobic conditions at $25^{\circ}C$. The rate of decolorization was determined by monitoring the decrease in the absorbance maxima of the dye by UV-visible spectroscopy. The decolorization of methyl violet was optimal at pH 5.5 and $30^{\circ}C$ when agitated at 200 rpm. Addition of glucose or arabinose (2%) as a carbon source and sodium nitrate or soyapeptone (0.2%) as a nitrogen source enhanced the decolorization ability of the culture. Furthermore, the culture exhibited a maximum decolorization rate of methyl violet after 24 h when the C:N ratio was 10. Nine N-demethylated decolorized products of methyl violet were identified based on UV-visible spectroscopy, Fourier transform infrared (FTIR), and LC-MS analyses. The decolorization of methyl violet at the end of 24 h generated mono-, di-, tri-, tetra-, penta-, and hexa-N-demethylated intermediates of pararosaniline. The variation of the relative absorption peaks in the decolorized sample indicated a linear decrease of hexa-N-demethylated compounds to non-N-demethylated pararosaniline, indicating a stepwise N-demethylation in the decolorization process.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.27 no.4
• /
• pp.494-498
• /
• 2000

A major concern in pediatric dentistry is maximizing risk management through optimal monitoring of respiratory function during sedation techniques, and Pulse Oximeter is one of the most popular devices for these purpose. Pulse Oximeter is non-invasive device for detecting the sensitive fraction between the saturated & desaturated hemoglobin. Several Studies proved that there is no significant difference between the $SpO_2$ and $SaO_2$. In this article, We examined three Pulse Oximeter $(3300MX^{(R)},\;OX90^{(R)},\;BPM200^{(R)})$. The Pulse Oximeter using shorter pulse beat averaging showed more sensitive reaction to the statue of the patient and lower saturation data. We compared pair of Pulse Oximeter applied to one patient at one time. and $BPM200^{(R)}$(using shorter pulse beat averaging) showed statistically low saturation.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.1 no.3
• /
• pp.33-40
• /
• 2008

The TMO may contain two types of methods, time-triggered methods(also called the spontaneous methods of SpMs) which are clearly separated from the conventional service methods (SvMs). The SpM executions are triggered upon design time whereas the SvM executions are triggered by service request message from clients. In this paper, we describes the application environment as the patient monitor telemedicine system with TMO structure. Vital sign information web viewer systems is also the standard protocol for medical image and transfer. In order to embrace new technologies as telemedicine service, it is important to develope the standard protocol between different systems in the hospital, as well as the communication with external hospital systems. It is able to apply to remote medical examination and treatment. Through the proper data exchange and management of patient vital sign information, real time vital sign information management will offer better workflow to all hospital employee.

• Geophysics and Geophysical Exploration
• /
• v.8 no.1
• /
• pp.92-96
• /
• 2005

Comprehensive field surveys, including various hydrogeological and geophysical methods, were carried out to appraise the applicability of those methods to a leakage problem at the Sandong earth fill dam in southwestern Korea. The methods applied in the fold site were tracer tests, monitoring of drawdown and leakage with discharge of reservoir water, electrical resistivity surveys using the dipole-dipole array, self-potential (SP), and temperature logging methods. The leakage pattern in the reservoir wall was demonstrated by hydrogeological methods and was further clarified by the geophysical surveys. Leakage turned out to be through the right abutment of the reservoir wall. In this study, we conformed that the electrical resistivity method is effective in detecting the zones favorable to leakage, and SP methods are useful for delineating the leakage pathways themselves, because leaks generate strong streaming-potential anomalies.

• Neonatal Medicine
• /
• v.28 no.1
• /
• pp.14-21
• /
• 2021

Purpose: Premature infants have immature respiratory control and cerebral autoregulation. We aimed to investigate changes in cerebral oxygenation during apnea with and without peripheral oxygen desaturation in premature infants. Methods: This prospective observational study was conducted at Inha University Hospital. Near-infrared spectroscopy (NIRS)-monitored regional cerebral oxygen saturation (rScO₂) and pulse oximeter-monitored peripheral oxygen saturation (SpO₂) were assessed during the first week of life in 16 stable, spontaneously breathing preterm infants. Apneic episodes that lasted for ≥20 seconds or were accompanied by desaturation or bradycardia were included for analysis. The average rScO₂ value during the 5-minute prior to apnea (baseline), the lowest rScO₂ value following apnea (nadir), the time to recover to baseline (recovery time), the area under the curve (AUC), and the overshoot above the baseline after recovery were analyzed. Results: The median gestational age and birth weight of the infants were 29.2 weeks (interquartile range [IQR], 28.5 to 30.5) and 1,130 g (IQR, 985 to 1,245), respectively. A total of 73 apneic episodes were recorded at a median postnatal age of 2 days (IQR, 1 to 4). The rScO₂ decreased significantly following apneic episodes regardless accompanied desaturation. There were no differences in baseline, nadir, or overshoot rScO₂ between the two groups. However, the rScO₂ AUC for apnea with desaturation was significantly higher than that for apnea without desaturation. Conclusion: Cerebral oxygenation can significantly decrease during apnea, especially when accompanied by reduced SpO₂. These results add the evidence for the clinical utility of NIRS in monitoring premature infants.

• Economic and Environmental Geology
• /
• v.56 no.2
• /
• pp.185-199
• /
• 2023

This study aimed to predict the effects of water ecology on the supply of hydrothermal energy to model a housing complex in Eco Delta Smart Village in Busan. Based on the results, engineering measures were recommended to minimize problems due to possible temperature variations on the supply of hydrothermal energy from the river. The current distribution of fish, benthic macroinvertebrates, and phytoplankton in the Pyeonggang Stream was monitored to determine their effects on water ecology. In the research area, five species and three families of fish were observed. The dominant species was Lepomis macrochirus, and the subdominant species was Carassius auratus. Twenty-five species and 21 families of benthic macroinvertebrates were found. The distribution of aquatic insects was poor in this area. The dominant species were Chironomidae sp., Lymnaea auricularia, Appasus japonicus, and Caridina denticulata denticulata in February, May, July, and October. Dominant phytoplankton were Aulacoseira ambigua and Nitzschia palea in February and May. Microcystis sp. was dominant in July and October. The health of the ecology the Pyeonggang Stream was assessed as D (bad) according to the benthic macroinvertebrate index (BMI). Shifts in the location of the discharge point 150 m downstream from intake points and discharge through embedded rock layer after adding equal amounts of stream water as was taken at the beginning were suggested to minimize water temperature variations due to the application of hydrothermal energy. When the scenario (i.e., quantity of water intake and dilution water, 1,600 m³/d and water temp. difference ±5 ℃) was realized, variations in water temperature were assessed at -0.19 ℃ and 0.59 ℃ during cooling and heating, respectively, at a point 10 m downstream. Water temperatures recorded at -0.20 ℃ and 0.68 ℃ during cooling and heating, respectively, at a point 10 m upstream. All stream water temperatures after the application of hydrothermal energy recovered within 24 hours. Future work on the long-term monitoring of ecosystems is suggested, particularly to analyze the influence of the water environment on hydrothermal energy supply operations.

• Parasites, Hosts and Diseases
• /
• v.62 no.1
• /
• pp.85-97
• /
• 2024

This study aimed to describe the morphological and molecular characteristics of Paralecithodendrium longiforme (Digenea: Lecithodendriidae) adults and cercariae isolated in Thailand. Adult flukes were isolated from the Chinese pipistrelle bat (Hypsugo sp.), and cercariae were detected in the viviparid snail (Filopaludina martensi martensi) from Chiang Mai province. The morphological characteristics were observed and described using conventional methods, and the molecular characteristics with internal transcribed spacer 2 (ITS2) and 28S rDNA gene sequences. The adult flukes were fusiform, 0.84-0.98 mm in length, and 0.37-0.49 mm in width, and were distinguishable from other species by the presence of longitudinal uterine coils. The cercariae were nonvirgulate xiphidiocercariae, with the oral sucker bigger than the acetabulum, the tail without fin fold, a body size of 117.5-138.3×48.3-52.2 ㎛, and a tail size of 100.7-103.7×15.0-18.9 ㎛. Molecular studies revealed that the adults and cercariae shared 99.3% (ITS2) and 99.6% (28S rDNA) homology with each other. They were phylogenetically close to P. longiforme with an identity of 94.5% for ITS2 and 98.7% for 28S rDNA. This study provides new information on the natural definitive host and first intermediate host of P. longiforme in Thailand. The discovery of its cercarial stage in Filopaludina snails highlights the importance of monitoring the associated second intermediate host and prevention and control of this potentially zoonotic trematode.