• Title/Summary/Keyword: SP 조사

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Conditions for the Pigment Production by Bacillus sp. CS-17 and Antibacterial Activity of Pigment Concentrated Extracts (Bacillus sp. CS-17의 색소 생성조건 및 색소 농축액의 항균특성)

  • Son, Dong-Hwa;Kwon, Oh-Jin;Choi, Ung-Kyu;Chung, Yung-Gun
    • Applied Biological Chemistry
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    • v.41 no.3
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    • pp.213-218
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    • 1998
  • A bacterium with potent activity of pigment production and protease was isolated and identified as being Bacillus sp. CS-17. Cell growth, protease activity and pigment production of the strain reached to its maximum point after 24 hrs, 48 hrs, 72 hrs, respectively. The best pigment producing ability of Bacillus sp. CS-17 was shown on basal medium for pigment production added 1.0% soybean. The high effcient conditions for pigment production was obtained at culture of pH 8.5, $37^{\circ}C$ and 72 hours. Among the tested 5 gram positive strains and 6 gram negative strains, weak antibacterial activity of pigment concentrated extracts was appeared against growth of B. subtilis, P. aeruginosa, S. typhimurium, E. aerogenes, B. cereus, A. hydrophila.

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Natural parasitism of parasitoids on Phyllocnistis citrella (Lepidoptera: Gracillaridae) in Jeju citrus orchards in Korea (제주 감귤원에서 귤굴나방에 대한 포식 기생자 천적의 자연 기생율)

  • Hyun, Seung Young;Lim, Un Taek;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.58 no.4
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    • pp.299-303
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    • 2019
  • This study was conducted to investigate the species of native parasitoids attacking Phyllocnistis citrella and their parasitism in citrus orchards in Jeju, Korea. During the survey period from 2013 to 2014, seven parasitoids belonging to Chalcidoidea were found: majority of the parasitoids were Sympiesis striatipes with 73% and Quadrastichus sp. with 22% in Eulophidae. The others were Neochrysocharis sp., Pnigalio sp., Holcopelte sp., Ageniaspis sp. (Encyrtidae) and Trichomalopsis sp. (Pteromalidae). Average parasitism rate reached 10.7% in orchards with spraying insecticide, and 32.2% in environmental-friendly managed orchards.

미생물비료 인산가용화균이 오이의 생산량에 미치는 영향

  • Lee, Tae-Geun;Park, Dong-Ha;Ju, Yeong-Jik
    • Proceedings of the Korean Society of Organic Agriculture Conference
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    • 2002.12a
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    • pp.74-82
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    • 2002
  • 난용성 인산염을 가용화 시키는 Penicillium sp. PS-113와 Lactobacillus sp. 를 이용하여온실에서 오이에 대한 생육 실험을 실시하였다. 실험 결과는 다음과 같다. Penicillium sp. PS-113 $5.0{\times}106cfu/ml$ + Lactobacillus sp. $6{\times}106cfu/ml $동시 처리구에서 평균 오이 길이는 대조구에 비하여 3cm 더 긴것으로 조사되었고, 오이 낱개의 무게는 310.6g으로 무처리구에 비하여 89.2g 이 증가하였으며, 오이 전체 생산량은 10681.7g로 무처리구와 비교해 볼 때 4517.3g이 많아 생산량이 73% 증가하였다. Penicillium sp. PS-113 $5.0{\times}105cfu/ml$ 단독처리와 Penicillium sp. PS-113 $5.0{\times}105cfu/ml$ + Lactobacillus sp. $6{\times}106cfu/ml$의 동시 처리구도 오이의 평균 무게와 길이에 있어 무처리구보다 약간씩 더 높았다. Penicillium sp. PS-113 $5.0{\times}105cfu/ml$ + Lactobacillus sp. $6{\times}106cfu/ml$의 동시처리구는 무처리에 비하여 오이의 수가 2개 적었으나 오이 전체 생산량은 무처리구에 비하여 Penicillium sp. PS-113 $5.0{\times}105cfu/ml$ 단독 처리구에서 53.7%, Penicillium sp. PS-113 $5.0{\times}104cfu/ml$ + Lactobacillus sp. $6{\times}106cfu/ml$ 동시 처리구에서 48.5% 증가되었다. 따라서 인산가용화균의 처리가 오이의 생산성을 증대시키는 것으로 나타났다.

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Fermentation Characteristics of Koji inoculated with Cordyceps sp. (동충하초를 이용한 코오지의 발효특성)

  • 권동진
    • Microbiology and Biotechnology Letters
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    • v.30 no.1
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    • pp.46-50
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    • 2002
  • To investigate the quality of Koji produced by Cordyceps sp., a mixed culture of Aspergillus oryzae and the Cordyceps sp. were inoculated in Koji making. Viable cell counts and counts of fungi spore during Koji making for 120 hours were increased rapidly within 24-48 hours, after then their changes were not shown significantly during Koji making. The activities of amylase and protease in Koji inoculated with the mixed culture of A. oryzae and Cordyceps sp. were superior to one with A. oryzae only. The sensory evaluation on the Koji with 0.5% (w/w) A. oryzae and 0.5% (w/w) Cordyceps sp. were evaluated to be superior to ones. The best quality of Koji is prepared from the mixture of Cordyceps sp. and A. oryzae with the ratio of 0.5% and 0.5% (w/w).

Turfgrass Nematodes Isolated from Some Golf Courses (몇몇 골프장에서 발견된 잔디선충)

  • 강영진;이동운;추호렬;권태웅;신종창;신홍균;최일근;최영연
    • Asian Journal of Turfgrass Science
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    • v.17 no.2_3
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    • pp.81-85
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    • 2003
  • The plant parasitic nematodes were surveyed from Zoysia matrella, Agrotis palustris, and Poa pratensis at Anyang Benest Golf Club, Dongrae Benest Golf Club, and Gapyeong Benest Golf Club and from A. palustris at Castle Pine Golf Club. Criconema sp., Ditylenchus sp., Helicotylenchus sp., Meloidogyine sp., Pratylenchus sp., Tylenchus sp., and Tylenchorhynechus sp. were separated from unhealthy turf areas. In addition, Tylenchorhynechus dubius was firstly recorded from A. palustris at the green of Castle Pine Golf Club. Meloidogyne sp., Helicotylenchus sp., Ditylenchus sp., and Pratylenohus were separated from Z. matrella, Meloidogyne sp. and Pratylenchus were from A. palustris, and Meloidogyne sp., Tylenchus sp., Tylenchorhynchus sp., Criconema sp., and Helicotylenchus sp. were from P. pratensis. Out of separated nematodes, Meloidogyne was the most serious nematode and widely distributed.

Identification of pathogen and actual culture state of king oyster mushroom (Pleurotus eryngii) (큰느타리버섯 주요재배시 실태조사 및 병원균 분리동정)

  • Ha, Tai-Moon;Chi, Jeong-Hyun;Ju, Young-Cheuol;Sung, Jae-Mo
    • Journal of Mushroom
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    • v.4 no.4
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    • pp.135-143
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    • 2006
  • We have investigated cultural circumstance and given condition of king oyster mushroom(Pleurotus eryngii) growing farmer. We collected many pathogens from King oyster mushroom growing farmer and identified with chemicobiological test and microscope. Most of investigated farmers neglected their's growing room cleaning and washing, after harvesting At pin-heading induction time, humidity degree in growing room was kept of high level and Air ventilation volume was so little that fruit-body formation ratio was low. The collected pathogens were twenty eight strains and identified with Pseudomonas sp., Trichoderma sp. mostly. During the spawn running time and pin-heading induction time, contamination by Trichoderma sp. occurred mostly, but during the fruit-body growing time, contamination by Pseudomonas sp., Erwinia sp. etc, occurred.

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Temporal and Spatial Distribution of Benthic Polychaetous Community in the northern Jinhae Bay (진해만 북부해역 저서다모류 군집의 시$\cdot$공간적 분포)

  • Lim, Kyeong-Hun;Shin, Hyun-Chool
    • Korean Journal of Environmental Biology
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    • v.23 no.3 s.59
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    • pp.238-249
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    • 2005
  • The present study was carried out to apprehend that the pollutants originating from Jinhae Industrial Complex affect benthic polychaetous community in the northern Jinhae bay. An investigation on the macrobenthic community in Jinhae bay was conducted in September, December of 2002 and March of 2003. The benthic fauna showed mean density of 2,151 ind. $m^{-2}$ in September of 2002, 2,427 ind. $m^{-2}$ in December of 2002 and 2,394 ind. $m^{-2}$ in March of 2003. Major faunal groups are polychaetes, crustaceans and mollusks, corresponding to $73.7\%,\;12.0\%\;and\;11.7\%,$ in total mean density during all of the sampling season, respectively. The most abundant species was Lumbrineris longifolia $(24.85\%),$ followed by Tharyx sp. $(21.70\%),$ Mesochaetopterus sp. $(6.20\%),$ Heteromastus filiformis $(5.39\%),$ Prionospio sp. $(5.18\%),$ Clycinde sp. $(4.29\%),$ etc. Tharyx sp. was the highest abundant species in September of 2002, and Lumbrineris longifolia was the dominant species in another sampling seasons. The density and the species number of polychaetes were high around Chori Is. and poor near Jinhae Industrial Complex area. Cluster analysis based on the species composition showed that Jinhae bay could be divided into three regions except in March of 2003. In December of 2002, there are very distinct regions by the cluster analysis. The density of benthic polychaetes in Jinhae bay was higher than that in the other coastal area of Korea, due to the predominance by some of opportunistic species, such as Lumbrineris longifolia, Tharyx sp. and Heteromastus filiformis, etc. It means that the study area were in the process of organic enrichment.

Microbiological Characteristics of Gamma Irradiated and Low-Salted Fermented Squid (감마선 조사된 저염 오징어젓갈 발효의 미생물균총 특성)

  • Kim, Dong-Ho;Kim, Jae-Hun;Yook, Hong-Sun;Ahn, Hyun-Joo;Kim, Jung-Ok;Sohn, Cheon-Bae;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1619-1627
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    • 1999
  • Microbiological characteristics of gamma irradiated low salt squid Jeot-gal were examined. Following the fermentation periods, total bacterial cell, Lactobacillus spp., Staphylococcus spp., Streptococcus spp., Pseudomonas spp. and yeast cell number were counted on their selective media and some acid forming bacteria and Pseudomonas spp. were identified. As the gamma irradiation dose increased, the microbial density of early fermentation phase was reduced and the growth rate was delayed. The repression effects on microbiological growth by gamma irradiation were to be higher as salt concentration increased. Adequate conditions of salt concentration and gamma irradiation for low-salt squid Jeot-gal preparation were 10% and 10 kGy, respectively. Lactobacillus sp. 2, Micrococcus varians and Streptococcus sp. I were isolated from 5% salt containing squid Jeot-gal, and Micrococcus morrhuae was from 20% only while Lactobacillus plantarum and Lactobacillus brevis were widespread. Lactobacillus brevis, Pediococcus halophilus and Pseudomonas diminuta were sensitive and Lactobacillus plantarum, Micrococcus morrhuae and Pseudomonas sp. 3 were resistant to gamma irradiation. The diversity of microflora decreased as salt concentration decreased and gamma irradiation dose increased.

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Characterization of the Biosurfactant-Producing Bacterium, Pseudoalteromonas sp. HK-3 Isolated from the Crude-Oil Contaminated Areas (원유로 오염된 지역으로부터 분리한 생물계면활성제 생산균주, Pseudoalteromonas sp. HK-3의 특성조사)

  • Cho, Su-Hee;Oh, Kye-Heon
    • Korean Journal of Microbiology
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    • v.46 no.4
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    • pp.346-351
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    • 2010
  • The purpose of this work was to investigate the characteristics of a biosurfactant-producing bacterium isolated from crude-oil contaminated soils. During the incubation of strain HK-3 with 1% crude-oil, bacterial growth pattern, the amount of biosurfactant production, and pH changes were monitored. In order to examine the effect of supplemented carbons on the production of biosurfactant, cultivation of HK-3 cells in BH media with different carbons (e.g. glucose, dextrose, mannitol, citrate, or acetate) revealed that the production of biosurfactant reached the maximal level at the 72 h incubation with mannitol, which the area of clear zone was measured to approximately 7.64 $cm^2$. Identification test using the BIOLOG system, morphology study based on scanning electron microscopy and the 16S rRNA sequence-based phylogenetic analysis assigned strain HK-3 to a Pseudoalteromonas species, designated as Pseudoalteromonas sp. HK-3 which was registered in GenBank as [FJ477041].