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Chemical Composition and Antioxidant Activity of Korean Buckwheat (Fagopyrum esculentum) Pollen Grain Collected by Honey Bee, Apis mellifera (메밀화분의 성분 특성 및 항산화 활성)

  • Hong, In-Pyo;Woo, Soon-Ok;Han, Sang-Mi;Lee, Mi-Kyoung
    • Journal of Apiculture
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    • v.32 no.3
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    • pp.261-268
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    • 2017
  • We evaluated the nutritional composition including proximate, amino acid, vitamin, minerals, and the antioxidant activity of buckwheat (Fagopyrum esculentum) pollen grains collected by Apis mellifera bees, to be used as a species of forage plant with Quercus acutissima (acorn) and Actinidia arguta pollen grains. The content of crude protein and fat were found 14.43% and 5.67%, respectively. Eighteen amino acids from buckwheat pollen were found, including 8 essential amino acids. The predominant amino acids were glutamic acid, aspartic acid and lysine, accounting for about 42.7% of total free amino acids. The concentration of vitamin C was the highest value of 13.7 mg/100g, followed by $B_3$ (niacin) and $B_1$ among the detected vitamins. The predominant minerals were potassium (1197.95mg/100g), followed by phosphorus (962.77mg/100g) and magnesium (535.42mg/100g), whereas copper, zinc and manganese were detected as minor elements. Antioxidant activity and total phenolic content accounted for 8.1% at $500{\mu}g/ml$ extract and $2.25{\mu}g/mg$, respectively.

Availability of the Optic Nerve Sheath Diameter Measured by Using Ultrasonography as a Secondary Survey for Patient with Head Injuries in the Emergency Department (응급실에 내원한 두부외상환자의 2차 평가로써 초음파를 이용한 시각신경집 지름 측정은 유용한가?)

  • Lee, Dong Wook;Lee, Jung Won;Park, Sae Hoon;Park, Ihl Sung;Lee, Hyun Jung;Yoo, Byeong Dae;Moon, Hyung Jun
    • Journal of Trauma and Injury
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    • v.26 no.3
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    • pp.104-110
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    • 2013
  • Purpose: Bedside ultrasonography is available in most emergency departments, and detecting the intracranial pressure is elevated is critical. Our objective is to evaluate the availability of bedside optic nerve ultrasound (ONUS) as a secondary survey for patients with head injuries in the emergency department (ED). Methods: From September, 2012, to March, 2013, we performed a prospective study of patients presenting to the ED after an accident. Patients with head injuries but without obvious ocular trauma or ocular disease were included. The ONUS was performed using a 3 to 12 MHz linear probe on closed eyelids after a primary survey. We analyzed the correlation between the brain computed tomography (CT) findings that suggested elevated intracranial pressure (ICP) and the Optic nerve sheath diameter (ONSD) measured by using ONUS. Results: A total of 81 patients were enrolled. Forty-seven had CT results consistent with elevated ICP, and their mean ONSD was $5.98{\pm}0.59$ mm; the mean ONSD of patients who showed no signs of elevated ICP on CT was $4.63{\pm}0.21$ mm. The sensitivity and the specificity for the ONSD, compared with elevated ICP, were 98.87% and 100%, respectively, when the cut-off value was set to 4.96 mm. The area under curve (AUC) was 0.997 in the receiver operating characteristic curve (ROC curve). Conclusion: An evaluation using ONUS is a simple noninvasive procedure and is a potentially useful tool as a secondary survey to identify an elevated ICP.

A Study on the Wear Sensation Cellulose Fabric in Summer (여름철 셀룰로오스 섬유 의복의 착용감에 관한 연구)

  • Kwon Soo-ae;Lee Soon-won
    • Journal of the Korean Society of Clothing and Textiles
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    • v.12 no.1 s.26
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    • pp.81-91
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    • 1988
  • The purpose of this study is to investigate the physiolosical responses and subjective wear sensation to the clothes with different cellulose composition. The experimental garments were four types of cellulosic fabrics, respectively composed of $100\%$-cotton, $100\%$-Rayon, cotton/Flax 85/15, and rayon/Flax 85/15 (weft blended), were sewn in blouses with half-length sleeves. Four healthy women were chosen for this experiment. The condition of the experimental room were in two environments: Temp., $25{\pm}1^{\circ}C$, R.H. $60{\pm}3\%$ and Temp., $30{\pm}1^{\circ}C$, R.H. $70{\pm}3\%$, and air velocity was maintained at 0.25 m/sec. The results are as follows. 1) At $25^{\circ}C$, rayon blouse showed the pleasantest feeling because of its excellent humidity sensation and tactile sensation. At $30^{\circ}C$, rayon and flax blended blouse showed most comportable sensation. In both environments, blouse of $100\%$ cotton showed the least pleasant because of high vapor pressure inside the blouse and bad tactile sensation. 2) Vapor pressure inside the blouse and tactile sensation play the most impotant role in comfort. Consequantly the blouse made of $100\%$-cotton which bring low air permeability, moisture regain and water vapor permearbility showed more unpleastness than rayon blouse because it raises temperature, relative humidity and vapor pressure inside the blouse under the hot circumstances In the conditions with much sweat, rayon and flax blended blouse with high stiffness increases comfort better than $100\%$ rayon because the former prevents sticking to the skin.

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Adsorption Characteristics of Toluene Gas Using Fluorinated Phenol-based Activated Carbons (불소화 처리된 페놀계 활성탄소를 이용한 톨루엔 가스흡착 특성)

  • Kim, Min-Ji;Jung, Min-Jung;Kim, Min Il;Choi, Suk Soon;Lee, Young-Seak
    • Applied Chemistry for Engineering
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    • v.26 no.5
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    • pp.587-592
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    • 2015
  • Activated carbons (ACs) were treated by fluorination to improve the adsorption property of toluene gas among volatile organic compounds (VOCs). The pore characteristics and surface properties of these activated carbons were evaluated by BET and XPS and the adsorption property and removal efficiency of toluene gas was investigated by gas chromatography. The breakthrough time of fluorinated ACs was increased about 27% compared to that of untreated ACs when the toluene gas of 100 ppm was flowed at a flow rate of $300cm^3/min$. Fluorinated AC of 0.1 g adsorbent totally adsorbed toluene gas in 100 ppm to 100 % during the adsorption time in 19 h. These results can be used as a treatment technology or removal of carcinogenic materials such as toluene.

Carotenoid Contents of Yellow Sweet Potatoes (황색고구마의 Carotenoid 색소 함량)

  • Kim, Seon-Jae;Rhim, Jong-Whan;Jung, Soon-Teck;Ahn, Young-Sup;Oh, Yong-Bee
    • Korean Journal of Food Science and Technology
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    • v.29 no.2
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    • pp.218-222
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    • 1997
  • Twelve varieties of yellow sweet potatoes were measured for carotenoids content to assess their potential as a source of natural food colorant. Benihayato variety had the highest content of carotenoids (20.2 mg/100 g fr wt) but other varieties ranged with $4.6{\sim}16.7\;mg/100\;g$ fr wt. Hunter's +a-values (redness) increased linearly $(R^{2}=0.826)$ with carotenoids content of yellow sweet potatoes. Absorption spectrum of carotenoids extracted from the Benihayato variety was similar to that of standard ${\beta}-carotene$ indicating that carotenoids in yellow sweet potato are mostly composed of ${\beta}-carotene$.

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Assessment of Nucleus-DNA Damage in Red Pepper Cells Treated with γ-Radiation through Comet Assay (Comet 분석을 통한 방사선처리 고추세포의 핵 DNA 손상평가)

  • An, Jung-Hee;Back, Myung-Hwa;Kim, Jae-Sung;Jeong, Jeong-Hag;Kwon, Soon-Tae
    • Journal of Plant Biotechnology
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    • v.31 no.3
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    • pp.225-230
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    • 2004
  • We employed single cell gel electrophoresis method (comet assay) to analyze the degree of nucleus-DNA damage in the leaves of red pepper (Capsicum annuum L.) seedlings exposed to $^{60}$ CO v-radiation stress. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length (H) in individual comet image isolated from pepper leaf cell. The T/H ratio of control-cells and treated-cells at 50 or 100 Gy were 1.28 and 3.54 or 3.39, respectively, suggesting that nuclei of pepper cells were severely damaged in the integrity of DNA strand by the treatment of enhanced v-radiation. The percentage of head-DNA in control-cells was 76.8%, whereas those of 50 and 100 Gy treated-cells were 55.9% and 59.9%, respectively. Pretreatment of low dose (4 to 20 Gy) radiation to seeds decreased DNA-damage in the leaves of seedlings treated with high dose radiation at 50 or 100 Gy. In this experiment, we developed a sensitive, reliable and rapid method for evaluating genotoxic effect in the nuclei of plant cells by employing comet assay.

A Study on the Meta Evaluation for Defense R&D Programs (국방Bt&D사업 자체평가시스템 메타평가)

  • Kim, Soon-Yeong;Ha, Kyu-Soo
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.32 no.3
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    • pp.59-70
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    • 2009
  • This study is the result of meta evaluation for the self evaluation of defense R&D programs in Korea by using meta evaluating indicators. The overall meta evaluation result of defense R&D programs gained 74.3 points out of 100, so it was evaluated as 'Good'. But it demonstrated that further improvement for overall system of defense R&D programs evaluation is required. And especially, it demonstrated that more alternatives are necessary in order to improve the utilizations and the feedbacks of evaluation results. The evaluation context component gained 80.2 points out of 100, so it was evaluated as 'Very Good'. The evaluation input component gained 73.1 points out of 100, so it was evaluated as 'Good'. The evaluation process component gained 74.8 points out of 100, so it was evaluated as 'Good'. And the evaluation outcome component gained 69.0 points out of 100, so it was evaluated as 'Good'. Basic model of meta evaluation was derived from the literature review and brain storming. And this meta evaluation model was determined by adopting the result of experts who performed evaluations for defense R&D programs in recent years. The reliability of components and items was verified by Cronbach's a coefficient. It was over 0.6 in evaluation components and items. And the reliability of evaluation context was 0.877, that of evaluation input was 0.755, that of evaluation process was 0.755, that of evaluation output was 0.755 respectively. From the analysis, it is attempted to identify possible problems and to find out the ways of improvements related to the self evaluation system of defense R&D programs. The ultimate objective of this study is to manage the programs effectively and improve the reliability and the objectiveness of the defense R&D programs.

Chemical Composition of Kiwifruits, Their Anti-microbial Activity and Their Hyperplasia Inhibition Effect of against Lung Cancer Cells (참다래의 이화학적 성분, 항균 활성 및 폐암 세포 증식 억제 효과)

  • Park, Yong-Seo;Lee, Gun-Soon;Towantakavani, Korsak;Park, Yun-Jum;Oh, Dae-Min;Heo, Buk-Gu
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.2
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    • pp.202-209
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    • 2009
  • This study was conducted to gather the basic data on the spread of the domestic kiwifruits, and the development of the manufactured goods and the health functional foods produced using kiwifruits. We determined the chemical compositions of four types of kiwifruits cultivated in Korea, Daeheung, Bidan, Haegeum and Hayward. In addition, we measured the anti-microbial activities and cytotoxicities of these types of kiwifruits. The vitamin C contents of the kiwifruits increased in the order of Bidan (93.82 mg/100 g), Daeheung (85.89 mg/100 g), Haegeum (83.73 mg/100 g) and Hayward (75.28 mg/100 g). The total amino acids contents per 100 g of kiwifruit (dry weight basis) were 483.97 mg (Haegeum), 453.08 mg (Hayward), 437.27 mg (Bidan) and 369.35 mg (Daeheung). The K and Ca contents of the kiwifruits ranged from 14.56~37.12 mg/L and 1.94~8.24 mg/L, respectively; however, the Fe, Mg, Zn and Cr contents all less than 1.83 mg/L. The antimicrobial activities of methanol extracts of kiwifruits against five gram positive bacteria at concentration of 2,000 mg/L in terms of inhibition diameter ranged from 8.8~12.8mm, while raged from 9.2~13.1mm against three gram negative strains of bacteria. The hyperplasia inhibition of lung cancer cells (Calus-6) by 800 mg/L kiwifruits extracts of Bidan, Haegeum, Daeheung and Hayward kiwifruits were 21.2%, 9.5%, 6.7% and 5.0%, respectively. Consequently, it was assumed that kiwifruits was rich in vitamin C, amino acids and K, and that they would therefore be useful in processed goods.

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Manufacture and Quality Evaluation of Cookies prepared with Mealworm (Tenebrio molitor) Powder (갈색거저리 유충 분말을 이용한 쿠키 제조 및 품질평가)

  • Min, Kyoung-Tae;Kang, Mi-Sook;Kim, Min-Ju;Lee, Sun-Hee;Han, Jung-soon;Kim, Ae-Jung
    • The Korean Journal of Food And Nutrition
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    • v.29 no.1
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    • pp.12-18
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    • 2016
  • This study was performed to develop and evaluate cookies prepared with various amounts (0 g, 12.5 g, 25 g and 37.5 g) of mealworm powder to serve as not only sports snacks but also for the general population as a new protein source. In the chromaticity of mealworm cookies, lightness and yellowness decreased whereas redness increased with more mealworm powder. The hardness of the mealworm cookies increased as more mealworm powder was added. In sensory evaluation, higher preference was shown with the measure of color, taste, and overall preference for mealworm cookies prepared with 50% mealworm powder (MP2). For the general composition of MP2, the moisture, carbohydrate, crude protein, crude fat and crude ash were higher compared with the control cookie. For the mineral contents of MP2, the contents of Ca (33.44 mg/100 g), P (225.13 mg/100 g), Mg (82.21 mg/100 g), Na (246.22 mg/100 g), and K (503.49 mg/100 g) were higher compared with the control cookie. The essential amino acids (valine, isoleucine, leucine, methionine, threonine, phenylalanine, and histidine) in MP2 were also higher compared with the control cookie. It was concluded that MP2 can be used as a new protein source for not only the maintenance of muscle but also for the prevention of muscle loss in old people.

Formation and Regeneration of protoplasts by Novozym 234 from Kluyveromyces fragilis N100 and Candida pseudotropicalis CBS607 (Novozym 234에 의한 Kluyveromyces fragilis N100과 Candida pseudotropicalis CBS607의 원형질체 형성과 재생)

  • Bai, Suk;Chun, Soon-Bai
    • Korean Journal of Microbiology
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    • v.22 no.1
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    • pp.49-56
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    • 1984
  • Formation and regeneration of protoplasts by Novozym 234 from Kluyveromyces fragilis N100 and Candida pseudotropicalis CBS607 were studied. This enzyme was more effective on cells grown at exponential phase than those at stationary one to convert intact cells into protoplasts. As osmotic stabilizer, ammonium sulphate was suitable for not only protoplast formation but also regeneration in K. fragilis as well as in C. pseudotropicalis. Optimal enzyme concentration was 3mg per ml in K. fragilis and 1~3mg per ml in C. pseudotropicalis, respectively. After the exposure of K. fragilis cells to 3mg per ml of enzyme for 3hr at 30$^{\circ}C$ , approximately 95% of protoplast formation of all observed cells was obgained, while about 100% from C. pseudotropicalis under the same condition was produced. The regeneration frequency of protoplasts by this enzyme was much lower than that by snail enzyme(Glusulase) although Novozym 234 converted cells from above two species into protoplasts free of cell debris effectively, compared with Glusulase. Novozym 234 appears to be suitable for subcellular fractionation to obtain nuclei or other organelles rather than protoplast regeneration.

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