To estimate potential use of fly ash in reducing $CH_4$ and $CO_2$ emission from soil, $CH_4$ and $CO_2$ fluxes from a paddy soil mixed with fly ash at different rate (w/w; 0, 5, and 10%) in the presence and absence of fertilizer N ($(NH_4)_2SO_4$) addition were investigated in a laboratory incubation for 60 days under changing water regime from wetting to drying via transition. The mean $CH_4$ flux during the entire incubation period ranged from 0.59 to $1.68mg\;CH_4\;m^{-2}day^{-1}$ with a lower rate in the soil treated with N fertilizer due to suppression of $CH_4$ production by $SO_4^{2-}$ that acts as an electron acceptor, leading to decreases in electron availability for methanogen. Fly ash application reduced $CH_4$ flux by 37.5 and 33.0% in soils without and with N addition, respectively, probably due to retardation of $CH_4$ diffusion through soil pores by addition of fine-textured fly ash. In addition, as fly ash has a potential for $CO_2$ removal via carbonation (formation of carbonate precipitates) that decreases $CO_2$ availability that is a substrate for $CO_2$ reduction reaction (one of $CH_4$ generation pathways) is likely to be another mechanisms of $CH_4$ flux reduction by fly ash. Meanwhile, the mean $CO_2$ flux during the entire incubation period was between 0.64 and $0.90g\;CO_2\;m^{-2}day^{-1}$, and that of N treated soil was lower than that without N addition. Because N addition is likely to increase soil respiration, it is not straightforward to explain the results. However, it may be possible that our experiment did not account for the substantial amount of $CO_2$ produced by heterotrophs that were activated by N addition in earlier period than the measurement was initiated. Fly ash application also lowered $CO_2$ flux by up to 20% in the soil mixed with fly ash at 10% through $CO_2$ removal by the carbonation. At the whole picture, fly ash application at 10% decreased global warming potential of emitted $CH_4$ and $CO_2$ by about 20%. Therefore, our results suggest that fly ash application can be a soil management practice to reduce green house gas emission from paddy soils. Further studies under field conditions with rice cultivation are necessary to verify our findings.
A field experiment was conducted in a moderately well drained paddy field doveloped in a narrow strip of a valley in order to observe changes of potassium requirement in paddy production when lime is applied. The results obtained are as follows; 1. The response of paddy to potassium (K) and lime (L) is represented mathematically as follow; $Y=462.78+11.582K-0.058L-0.768K^2-0.000015L^2+0.2204KL$. It is considered that the increase of potassium reqirement when slaked lime is applied, is partially due to the increased growth of plant accomplished by the improvement of soil conditions such as the reduction of respiration inhibitors and cationic balance in soil solution. 2. An economic analysis of the use of potassium and slaked lime applying the costs, 80 won per kg of paddy, 19 won per kg of potassium and 4 won per kg of slaked lime to the response function above, showed that the slaked lime without potssium brought a large loss, whereas the use of the lime together with potassium increased the profit remarkably. The profit increased when 10kg of potassium per 10 a is applied in addition to 200kg slaked limn, per 10kg is amounted 4,685 won. 3. A linear relationship between the economic optimum dose of potassium (y) and the amount of slaked lime (x) in paddy production, is obtained as follow; $$y=7.48+\frac{2.77}{200}x$$ It is, however, considered that the amount of potassium to he used might differ according to the soil conditions such as the potassium content and cation exchange capicity of the soil.
Aerenchyma development in rice (Oryza sativa L.) roots is quite important for adaptation to waterlogged or reduced soil conditions. Anatomical observations were carried out to clarify the development of schizogenous and lysigenous aerenchyma in elongating crown roots of rice. The crown roots of 3rd and 4th phytomer were taken from rice plants of the 8th leaf stage grown by hydroponic culture. The schizogenous intercellular spaces in the cortex of crown root tip were observed using a light microscope with semi ultra-thin sections and the lysigenous aerenchyma in mature tissue of crown root were observed using a cryo scanning electron microscope (cryo-SEM) with freezing fracture method. The schizogenous intercellular spaces in the root tip exist obviously in the middle portion of cortical cell layers close to the root-root cap junction, but not in root cap, stele and outer cell layers of cortex. The air spaces were formed at the junction of four neighbouring cells of inner cortex in the transverse sections, and between longitudinal cell layer connected along the root axis. Although many of those spaces were filled with liquid, some spaces seem to exist as air spaces. The lysigenous aerenchyma in the cortex, which hardly filled with liquid, emerged at 3-4 cm segment from the root tip and increased toward the basal region of root axis. The developing process of lysigenous aerenchyma was primarily separation of a radial row of cells caused by the shrinking and collapsing of cortical cells and then formation of septa along the radial cell rows by the fusion of cell wall with each other. These results suggest that the schizogenous and lysigenous aerenchyma playa role as a passage for the movement of oxygen into the root tip region where oxygen is required for respiration.
The object of this study was to investigate nitrogen fixation activity of rhizobium inoculated at seed coat when drought condition was applied in flowering period of soybean c.v. Samnamkong. The rhizobia used in this experiment were indigenous rhizobium, R2l4, RJl-29, USDA110 and USDA122. The experiment was done with 1/2000 Wagner pots in laboratory and greenhouse and was tested in completely randomized design with four replications. Nitrogen fixation activity in coventional culture was the highest in R2l4 and indigenous rhizobium among the five rhizobia strains. As given drought condition from flowering to maturity, nitrogen fixation activity was higher in R2l4 and RJl-29 than indigenous rhizobium. Leaf area and relative index (drought/convention) of pod weight were higher in USDA122, RJl-29 and R2l4 than indigenous rhizobium as given drought condition from flowering to maturity. High positive correlation was observed between nitrogen fixation activity of rhizobium and relative index(drought/convention) of pod weight. High negative correlation was observed between respiration of plant and relative index (drought/convention) of leaf area.
Proceedings of the Korean Society of Crop Science Conference
/
2017.06a
/
pp.281-281
/
2017
Recently, global warming by greenhouse gas effect is getting danger and danger for human life and agriculture at present. In Indonesia, according to heavy rain in the agriculture land is often covered by excess water in result crop growth would be affected negative. This water stress triggers roots failure in anaerobic condition for upland crop because of limiting roots respiration. Chili pepper grows in upland sometimes in touch with waterlogging due to rainfall and /or over flow water from river in Indonesia. In this case, roots growing is inhibited by effect of shortage of oxygen at root cap. Therefore, the objective of this study is to observe the plant behavior in waterlogging using mahor local genotypes (Ferosa, Laris, Romario) in Sumatra. The experiment was kept by at 1cm depth water above the soil surface as a waterlogged treatment for ---days. As a result, waterlogging affected plant growth of chili negatively, especially for roots growth. Almost roots were getting bad and changed color for brown during waterlogging. A significant negative effect for nutrient absorption by roots was found in dry weight of all varieties during waterlogging. Dry weight of roots was decreased by 81.4% and 67.6%, and those of aerial part decreased by 74% and 67.2% compared with control in Ferosa and Romario at 1week after treatment. On the other hand, dry weight of roots was decreased only 35% in Laris. Therefore, Laris has a tolerance for waterlogging compared to with other varieties. Also, Laris in SPAD value was kept initial level during waterlogging however those of Ferosa and Romario decreased. Finally, due to impact of waterlogging, it may be the roots become failure because of less aerenchyma formation under anaerobic condition. We need confirm aerenchyma formation morphologically in the future.
Journal of the Korean Institute of Landscape Architecture
/
v.41
no.6
/
pp.140-146
/
2013
This experiment was conducted in order to study the physiological characteristics of Sedum takevimense in different moisture conditions. The photosynthetic rate, water use efficiency and the respiratory rate were determined by using a photable photosynthesis system. According to the results, the best illumination range and moisture range were explicitly selected. The highest photosynthetic rate was at $600{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and after this value, the trend showed a reduction. When the moisture was 11.31%, the photosynthetic capacity and water use efficiency reached maximum value, but the respiratory rate reached maximum value at 7.91%. According to the measured values, the best illumination range was $600{\sim}1,200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and the best moisture range was 7.09~11.31%.
Proctor, John T.A.;Palmer, John W.;Follett, John M.
Journal of Ginseng Research
/
v.34
no.3
/
pp.175-182
/
2010
North American ginseng seedlings (Panax quinquefolius L.) were grown in pots in heated greenhouses, in a cool greenhouse, or in the field, in 11 experiments at various times over 16 years. Crop establishment, dry matter partitioning, photosynthesis, radiation use efficiency and carbon budget were measured and/or calculated in some years. Once the seedling canopy, of about $20\;cm^2$ per seedling, and a leaf area index of 0.37, was established, about 40 days after germination, full canopy display lasted about 87 days. Only 16.6% of the incoming solar radiation was intercepted by the crop, the remainder falling on the mulched soil surface. Total and root dry matter accumulations in the cool greenhouse and in the field were about double that in the heated greenhouses. Partitioning of dry matter to roots (economic yield or harvest index) in the cool greenhouse and in the field was 73% whereas it was 62.5% in the heated greenhouses. The relationship between root dry matter and radiation interception during the full canopy period was linear with growth efficiencies of $2.92\;mg\;MJ^{-1}$ at 4.8% of incoming radiation and $0.30\;mg\;MJ^{-1}$ at 68% of incoming radiation. A photosynthetic rate of $0.39\;g\;m^{-2}\;h^{-1}$ was attained at light saturation of about $150\;{\mu}mol\;m^{-2}\;s^{-1}$ (7.5% of full sunlight); dark respiration was $0.03\;g\;m^{-2}\;h^{-1}$, about 8.5% of maximum assimilation rate. Estimates of dry matter accumulation by growth analysis and by $CO_2$ uptake were similar, 6.21 vs. 7.62 mg $CO_2$, despite several assumptions in $CO_2$ uptake calculations.
Chinese fir (Latin name: Cunninghaimia lanceolata) is one of the major commercial coniferous trees. Most of Chinese fir forests are managed in successive rotation sites, which lead productivity to decline. Autotoxicity is the important reason for soil degradation of Chinese fir plantation, especially, phenolic acids are considered as the major allelopathic toxins which induce autotoxicity in Chinese fir rotation stands. We performed here proteomic approach to investigate the response of proteins in Chinese fir leaves to salicylic acid. The tube plantlets of Chinese fir clone were treated with 120 mg/L salicylic acid for 1, 3 and 5th day. 2-DE, coupled with MALDI-TOF-TOF/MS, was used to separate and identify the responsive proteins. We found 12, 7, and 12 candidate protein spots that were up- or down-regulated by at least 2.5 fold after 1, 3, and 5th day of the stress, respectively. Of these protein spots, 16 spots were identified successfully. According to the putative physiological functions, these proteins were categorized into five classes (1) the proteins involved in protein stability and folding, including 26S proteome, Grp78, Hsp70, Hsp90 and PPIase; (2) the protein involved in photosynthesis and respiration, including OEC 33 kDa subunit, GAPDH; (3) the protein related to cell endurance to acid, F-ATPase; (4) the protein related to cytoskeleton, tubulin; (5) the protein related to protein translation: prolyl-tRNA synthetase. These results give new insights into autotoxic substance stress response in Chinese fir leaves and provide preliminary footprints for further studies on the molecular signal mechanisms induced by the stress.
This study was conducted to compare the regeneration of the seedlings under different vegetation types and to identify the presence of allelopathy in Abies koreana(Ak) natural forest in Banyabong Peak(elevation, 1715m), of Mt. Chiri. Twenty quadrats($10m{\times}10m$) were placed in May, 1996 to classify vegetation structure using TWINSPAN. Water-soluble extracts from leaves and soil humus of different vegetation types were collected to test their effects on both seed germination of Ak and mycelial growth of ectomycorrhizal fungi. Phenolic compounds from soil humus were quantified using HPLC. Among the four vegetation types, Sasa borealis(Sb) was found in both Ak-Quercus mongolica(Qm) and Ak-Rhododendron schlippenbachii(Rs) communities. Natural seeding of Ak was $230,000{\pm}90,000seeds/ha$ in 1995 and their germination rate was 25% in an ideal laboratory condition. Density of Ak seedlings less than 5cm in height was 52,000/ha in 1996, while that of seedlings taller than 5cm in height was only 4,000/ha. In the case of Ak-Qm community, density of Ak seedlings with Sb understory was only 7% of the density of seedlings with Rs understary, suggesting the inhibitory effect of Sb. The germination rate of Ak seeds was significantly reduced by leaf extracts of Sb, and Rhododendron mucronulatum var. ciliatum(Rm) and Ak. Soil humus extract of Ak-Qm-Sb subcommunity reduced germination of Ak seeds by 81% and also reduced by 19% the respiration of mycelia of ectomycorrhizal fumgus, Lactarius sp. Among the seven phenolic compounds identified from the soil humus, extract, Ak-Qm-Sb subcommunity contained significantly high content of p-hydroxybenzoic acid, vanillic acid, and syringic acid. Particularly, p-hydroxybenzoic acid was present at 4.2ppm in the Sb roots and at 16.5ppm in the Sb humus, suggesting that it could be the primary allelopathic compound in Abies koreana forests with Sasa borealis understory.
Commercial Bio- Green(B.G.) functional water was manufactured through a series of processes : water - ultra-purification - adding catalysts - energy imprinting fermenting with energized water + zeolite and others + photosynthetic bacteria in fermenter longrightarrow filtering. Control(0), 5 or 10 liters per plant of B.G. functional water were supplied to the orchard soil under canopy of 10 year- old ‘Tsugaru’/M26 apple trees on March 20, May 20 and June 20, 1995, respectively. pH and content of Ca and Mg of orchard soil were increased by supply with B.G. functional water. However, P$_2$$O_{5}$, K, and B contents were not influenced by the treatment. At harvest time soluble solid content of flesh tissue and anthocyanin of fruit skin were increased by the treatment. B.G functional water treatment showed higher root activities, and photosynthesis of leaves than that of control. Also B.G. functional water treatment enhanced Ca content in fruit skin and flesh tissues, whereas not affected N, K, and Mg contents. During storage at 4$^{\circ}C$ cold room, the more volume of B.G. functional water supply showed lower bitter pit symptom. Respiration and ethylene evolution in fruit decreased, while fruit firmness increased by the treatment during storage.
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