• Korean Journal of Acupuncture
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• 제24권4호
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• pp.181-200
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• 2007

Objevtives & Methods : Effects of cultivated wild ginseng pharmacopuncture at BL18 and LI11 on lipid composition, cytokine level, liver function, anti-oxidative capacity and histological characters were investigated in diet-induced obese rats. Forty male Sprague-Dawley rats weighing about 400g were divided into 4 groups of control, BL18, LI11 and BL18 plus LI11 pharmacopuncture groups and raised for 4 weeks. Results : 1. Plasma ${\beta}$-lipoprotein, free fatty acids level and TNF-${\alpha}$ levels significantly decreased in the pharmacopuncture groups compared to those of no treatment group. Plasma and liver total cholesterol, triglyceride, glucose and thiobarbituric acid reactive substance (TBARS) levels were also significantly lower than those of no treatment group. There was, however, no difference in TBARS level among pharmacopuncture groups. Liver total cholesterol level of BL18 pharmacopuncture group was lower than those of the other two pharmacopuncture groups. In LDL-cholesterol level, BL18 pharmacopuncture and BL18 plus LI11 pharmacopuncture groups only had significantly lower levels than that of no treatment group. 2. There was no significant difference between cultivated wild ginseng pharmacopuncture groups and no treatment group in IL-6, alanine transaminase (ALT), aspartic acid transaminase (AST) levels. 3. Compared with \ those of no treatment group, pharmacopuncture groups had significantly higher levels of HDL-cholesterol, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase activities. There was, however, no significant difference among pharmacopuncture groups. 4. Histological characters of heart, kidney and liver of BL18 pharmacopuncture group were similar to those of normal rats. Conclusions : These results indicate that cultivated wild ginseng pharmacopuncture at BL18 and LI11 may suppress adipose tissue mass and lipid peroxidation and activate antioxidant system.

• 한국식품과학회지
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• 제39권2호
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• pp.217-221
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• 2007

인삼 분말이 $B({\alpha})P$의 투여로 간 독성이 유발된 마우스에서의 항산화 효소, 글루타치온 및 과산화지질 함량 변화에 미치는 영향으로 살펴보았다. 먼저 인삼 분말의 투여시 $B({\alpha})P$ 투여로 인한 간 조직중의 SOD, catalase 그리고 GSH-Px의 활성은 유의적으로 증가되었다가, 인삼분말의 처리로 이들 활성이 유의적으로 감소하였다. 반면, GST 활성과 간 조직중의 글루타치온 함량은 $B({\alpha})P$ 단독 투여군에서는 감소되었다가 인삼 분말 투여시 유의적인 증가를 보였다. 그러나 cytochrome P-450 활성과 지질과산화물 함량은 $B({\alpha})P$ 투여시 증가되었다가 인삼 분말의 투여시 유의적으로 감소되었다. 이상의 결과로 인삼 분말은 $B({\alpha})P$에 의한 간 손상에 대한 보호효과를 가지는 것으로 사료된다.

• 동의신경정신과학회지
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• 제10권1호
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• pp.79-93
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• 1999

This experiment was done to investigate the effects of Ojajiwhangeumja(OJWEJ) to the symptoms of senility. To prove the effects of OJWEJ we injected scopolamine(1mg/kg) to the vein of rats. And we measured the blood cells(WBC, RBC, Platelet), constituents of serum(BUN, creatinin, glucose, uric acid), endurance of films of the red blood cell to erythrocyte hemolises, the activity of cholinesterase in serum, TBA and the activity of catalase, SOD in the purified microsome of brain tissue of rats. The results were as follows; 1. The number of white blood cells, platelet was increased significantly in the group treated by OJWEJ in comparison with control group. 2. The number of BUN, creatinin, glucose of serum decreased significantly in the OJWEJ treated group compared with control group. 3. The erythrocyte hemolises in red blood cells restrained significantly in the group treated by OJWEJ in comparison with control group. 4. The activity of cholinesterase in OJWEJ treated group increased significantly compared with control group. 5. The amounts of malondialdehyde of serum decreased significantly in the OJWEJ treated group in comparison with control group. 6. The catalase in the microsome of rat brain was activated significantly in the group treated by OJWEJ compared with control group. 7. The superoxide dismutase in the group treated by OJWEJ activated significantly in comparison with control group. According to this experiment it is suggested that OJWEJ accelerates the activity of colinesterase and restrains the creation of erythrocyte hemolises and accumulation of senile substance. But I look forward to see the further research to be made.

• 생약학회지
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• 제35권4호통권139호
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• pp.293-299
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• 2004

The current investigation was carried out to find out the anticancer activity of the methanol extract from Buplerum falcatum against cancered ICR mouse and cancer cell lines such as J774A.1 and L1210 cells. Extract of Buplerum falcatum displayed the considerable augmentation(134%) of the survival of ICR mouse bearing Sarcoma 180 cancer. In addition, the cytotoxic effects of methanol extract of Buplerum falcatum against J774A.1 cells and L1210 cells were found to show $IC_{50}$ values of $57.3\;{\mu}g/ml$ and $54.6\;{\mu}g/ml$, respectively. In contrast to such cytotoxicity against cancer cells, the extract exerted only meagre toxicity against normal lymphocytes. The increased generation of $O_2^-$ and the considerably increased activities of super-oxide dismutase(SOD) and glutathione peroxidase(GPx) of both J774A.1 cells and L1210 cells in the presence of Buplerum falcatum extract implied that the observed cytotoxicities may have resulted from the detrimental effect of reactive oxygen species(ROS) evoked by Buplerum falcatum extract on the cancer cells.

• Biomolecules & Therapeutics
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• 제26권6호
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• pp.568-575
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• 2018

In order to discover lifespan-extending compounds made from natural resources, activity-guided fractionation of Zingiber officinale Roscoe (Zingiberaceae) ethanol extract was performed using the Caenorhabditis elegans (C. elegans) model system. The compound 6-gingerol was isolated from the most active ethyl acetate soluble fraction, and showed potent longevity-promoting activity. It also elevated the survival rate of worms against stressful environment including thermal, osmotic, and oxidative conditions. Additionally, 6-gingerol elevated the antioxidant enzyme activities of C. elegans, and showed a dose-depend reduction of intracellular reactive oxygen species (ROS) accumulation in worms. Further studies demonstrated that the increased stress tolerance of 6-gingerol-mediated worms could result from the promotion of stress resistance proteins such as heat shock protein (HSP-16.2) and superoxide dismutase (SOD-3). The lipofuscin levels in 6-gingerol treated intestinal worms were decreased in comparison to the control group. No significant 6-gingerol-related changes, including growth, food intake, reproduction, and movement were noted. These results suggest that 6-gingerol exerted longevity-promoting activities independently of these factors and could extend the human lifespan.

• 한국조경학회지
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• 제34권6호
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• pp.39-53
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• 2007

Unlike conventional roof landscaping, where various kinds of plants and structures are employed, a grass roof is a roof on which herbaceous plants are grown in planting medium and which is not accessed or maintained, mainly because it doesn't have sufficient load capacity to support a regular roof garden. They are mostly built on existing roofs, whether flat slab or gabled. Planting on roofs has numerous advantages, such as creating a biotope, purifying urban air, adding moisture to the atmosphere, storing rain water, preventing flash floods, reducing energy use for heating and air conditioning, enhancing the urban landscape and providing relaxation to the city dwellers, not to mention the alleviation of global warming by absorbing $CO_2$. In addition to the general merits of roof planting, the grass roof has its own unique qualities. Only herbaceous species are planted on the roof, resulting in light weight which allows roofs of existing buildings to be planted without structural reinforcement. The species chosen are mostly short, tough perennials that don't need to be maintained. These conditions provide an ideal situation where massive planting can be done in urban areas where roofs are often the only and definitely the largest space available to be planted. If roofs are planted on a massive scale they can play a significant role in alleviating global warming, heat island effects and energy shortages. Despite the advantages of grass roofs, there are some problems. The most significant problem is the invasion of neighboring plants. They may be brought in with the planting medium, by birds or by wind. These plants have little aesthetic value comparing to the chosen species and are usually taller. Eventually they dominate and prevail over the original species. The intended planting design disappears and the roof comes to look wild. Since the primary value of a grass roof is ecological, a change in attitude towards what constitutes beauty on the roofscape is necessary. Instead of keeping the roof neat through constant maintenance, people must learn that the wild grass with bird's nests on their roof is more beautiful as it is.

• Biomolecules & Therapeutics
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• 제23권6호
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• pp.582-589
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• 2015

Several theories emphasize that aging is closely related to oxidative stress and disease. The formation of excess ROS can lead to DNA damage and the acceleration of aging. Vigna angularis is one of the important medicinal plants in Korea. We isolated vitexin from V. angularis and elucidated the lifespan-extending effect of vitexin using the Caenorhabditis elegans model system. Vitexin showed potent lifespan extensive activity and it elevated the survival rates of nematodes against the stressful environments including heat and oxidative conditions. In addition, our results showed that vitexin was able to elevate antioxidant enzyme activities of worms and reduce intracellular ROS accumulation in a dose-dependent manner. These studies demonstrated that the increased stress tolerance of vitexin-mediated nematode could be attributed to increased expressions of stress resistance proteins such as superoxide dismutase (SOD-3) and heat shock protein (HSP-16.2). In this work, we also studied whether vitexin-mediated longevity activity was associated with aging-related factors such as progeny, food intake, growth and movement. The data revealed that these factors were not affected by vitexin treatment except movement. Vitexin treatment improved the body movement of aged nematode, suggesting vitexin affects healthspan as well as lifespan of nematode. These results suggest that vitexin might be a probable candidate which could extend the human lifespan.

• Journal of Microbiology and Biotechnology
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• 제19권8호
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• pp.803-809
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• 2009

Recent evidence has revealed the occurrence of an apoptotic phenotype in Candida albicans that is inducible with environmental stresses such as acetic acid, hydrogen peroxide, and amphotericin B. In the present study, we found that the Chinese herbal medicine Baicalein (BE), which was one of the skullcapflavones, can induce apoptosis in C. albicans. The apoptotic effects of BE were detected by flow cytometry using Annexin V-FITC and DAPI, and it was confirmed by transmission electron microscopy analysis. After exposure to 4 ${\mu}g$/ml BE for 12 h, about 10% of C. albicans cells were apoptotic. Both the increasing intracellular levels of reactive oxygen species (ROS) and upregulation of some redox-related genes (CAP1, SOD2, TRR1) were observed. Furthermore, we compared the survivals of CAP1 deleted, wild-type, and overexpressed strains and found that Cap1p attenuated BE-initiated cell death, which was coherent with a higher mRNA level of the CAP1 gene. In addition, the mitochondrial membrane potential of C. albicans cells changed significantly (p<0.001) upon BE treatment compared with control. Taken together, our results indicated that BE treatment induced apoptosis in C. albicans cells, and the apoptosis was associated with the breakdown of mitochondrial membrane potential.

• Environmental Analysis Health and Toxicology
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• 제22권2호통권57호
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• pp.147-156
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• 2007

Six week-old ICR mice were divided into four groups including NC, RC, RR and RS, then they were injected with sun ginseng (RS), red ginseng (RR) and saline (RC) intraperitoneally as an amount of 60mg/kg body weight at 1 hour, 12 hours and 36 hours before the whole body irradiation with 6.5Gy of X-ray. And the mice were sacrificed at nine days after the irradiation. The RS group were significant increase in the weight of spleen (p<0.05) and the numbers of endogenous spleen colony (p<0.001) and WBC (p<0.05) especially neutrophils, lymphocytes and monocytes in comparison with the RC group. The RR group were significant increase in the numbers of endogenous spleen colony (p<0.01) and WBC (p<0.05) especially neutrophils, lymphocytes and eosinophils in comparison with the RC group. The RS group exhibited a more increase in the weight of spleen and the number of endogenous spleen colony than the RR group. The values of ALT (alanine transaminase) and AST (aspartate transaminase) were significantly elevated (p<0.05) by radiation and they were significantly decreased (p<0.05) in the RS group to the values of the NC group. The value of TBARS(thiobarbituric acid reactive substance) was significantly elevated (p<0.01) by radiation and it was significantly decreased (p<0.05) in the RS group. And the values of SOD (superoxide dismutase) and CAT (catalase) were significantly increased (p<0.05) in the RS group in comparison with the RC group. Taken together the above results, sun ginseng demonstrated the protective effects on hepatocytes and immune and hematopoietic cells in mice exposed to medium dose of irradiation, and those radioprotective effects were a little higher or similar to the red ginseng.

• The Korean Journal of Physiology and Pharmacology
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• 제20권6호
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• pp.613-619
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• 2016

Diabetic cardiomyopathy (DCM), a serious complication of diabetes mellitus, is associated with changes in myocardial structure and function. This study sought to explore the ability of insulin-like growth factor-1 (IGF-1) to modulate DCM and its related mechanisms. Twenty-four male Wistar rats were injected with streptozotocin (STZ, 60 mg/kg) to mimic diabetes mellitus. Myocardial fibrosis and apoptosis were evaluated by histopathologic analyses, and relevant proteins were analyzed by Western blotting. Inflammatory factors were assessed by ELISA. Markers of oxidative stress were tested by colorimetric analysis. Rats with DCM displayed decreased body weight, metabolic abnormalities, elevated apoptosis (as assessed by the bcl-2/bax ratio and TUNEL assays), increased fibrosis, increased markers of oxidative stress (MDA and SOD) and inflammatory factors (TNF-${\alpha}$ and IL-$1{\beta}$), and decreased phosphorylation of Akt and glycogen synthase kinase (GSK-$3{\beta}$). IGF-1 treatment, however, attenuated the metabolic abnormalities and myocardial apoptosis, interstitial fibrosis, oxidative stress and inflammation seen in diabetic rats, while also increasing the phosphorylation levels of Akt and GSK-$3{\beta}$. These findings suggest that IGF-1 ameliorates the pathophysiological progress of DCM along with an activation of the Akt/GSK-$3{\beta}$ signaling pathway. Our findings suggest that IGF-1 could be a potential therapeutic choice for controlling DCM.