• Journal of Microbiology and Biotechnology
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• v.1 no.4
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• pp.288-292
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• 1991

The aim of the current study is to evaluate the effects of medium compositions on the production of protease inhibitor in Streptomyces sp. SMF13. The production of protease inhibitor was counter-currently linked to extra-cellular protease, which were regulated by the culture conditions. Nitrogen source was the most critical ingredient affecting the production of protease inhibitor and protease. Carbon source was an important factor to determine the culture pH which affected very clearly the formation of protease and protease inhibitor. Inorganic phosphate inhibited the protease inhibitor production which was linked to the cell growth rate, although the optimal conditions for the production of protease inhibitor were not favouring to the cell growth.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.05a
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• pp.630-632
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• 2018

The compensation characteristics of the distorted WDM channels compensated for by dispersion management (DM) and optical phase conjugation in the long-haul (50 fiber spans ${\times}$ 80 km) transmission link with the randomly distributed single mode fiber (SMF) length and residual dispersion per spans (RDPS) for implementing of the flexible link configuration are investigated.

• Journal of Microbiology
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• v.35 no.2
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• pp.97-102
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• 1997

Cysteine desulfhydrase (EC 4.4.1.1.) was purified from the culture supernatant of Streptomyces albidoflavus SMF301 by hydroxyapatite, gel filtration and Resource Q ion-exchange chromatography with a purification fold of six identical subunits. The enzyme was stabilized by dithiothreitol and pyridoxal 5'-phosphate during the purification procedures. The optimum pH and temperature were pH 8.6 and 35$^{\circ}C$, respectively. The N-terminal amino acid sequence was identified as A-P-L-P-T-A-D-V-R-S-D-P-G-Y-R-E-W-L-G-E-A-V. The purified cystein desulfhydrase had a high substrate specificity toward cysteine, and exhibited no cystahionine $\gamma$-lyase activity. The $K_m$ value for cysteine was determined to be 0.37 mM.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.05a
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• pp.625-626
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• 2015

The optimal distribution pattern for the compensation for the distorted WDM signals due to the group velocity dispersion (GVD) and the nonlinear Kerr effects is induced in $80km{\times}50spans$ optical link with an artificial distributions of single mode fiber (SMF) lengths and residual dispersion per span (RDPS).

• Korean Journal of Microbiology
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• v.30 no.4
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• pp.278-285
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• 1992

Chemotaxonomic and numerical identification were carried out for a isolate of Streptomyces strain SMF301 producing spores in submerged culture. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate SMF301 was identified to cluster 1A of Streptomyces and best matched to Streptomyces limosus which is a synonym of Streptomyces albidoflavus. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces alidoflavus.

• Journal of Microbiology and Biotechnology
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• v.2 no.3
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• pp.220-225
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• 1992

Chemotaxonomic and numerical identification were carried out for an isolate of Streptomyces strain SMF13 producing thiol protease inhibitor. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate SMF13 was identified to be a member of the cluster 5 of Streptomyces and best matched to Streptomyces omiyaensis which is a synonym of Streptomyces exfoliatus. Therefore. it was concluded that the isolate was identified to be a strain of Streptomyces exfoliatus.

• Journal of Microbiology and Biotechnology
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• v.3 no.3
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• pp.174-180
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• 1993

A strain SMF14 producing an extracellular $\beta$-lactamase was isolated from soil and identified to be a strain of Streptomyces aureofaciens. $\beta$-Lactamase was purified from the cell free culture broth through batchwise hydroxyapatite adsorption, anion exchange chromatography on DEAE Sephadex A-50, gel filtration on Sephadex G-75, and adsorption chromatography on hydroxyapatite. The molecular mass was estimated to be about 43 kDa by SDS-PAGE. The $\beta$-lactamase had substrate specificity to penicillins and it was inhibited by clavulanic acid, being classified to the group 2a of penicillinase.. The optimal reaction pH and temperature were pH 6.0~7.5 and $50^{\circ}C$. The $K_m, and V_{max}$ values of $\beta$-lactamase for penicillin G were calculated to be 1.72 mM and 5.4$\times$$10^5 \mu M \cdot min^{-1}$, respectively.

• YAKHAK HOEJI
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• v.39 no.3
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• pp.306-313
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• 1995

The aim of the present study was to produce low molecular weight cathepsin B inhibitor. A strain of Streptomyces aburabiensis isolated from soil in Korea was selected and the optimum condition for the production of the inhibitor was evaluated. Glucose and soytone were selected as best carbon and nitrogen sources, respectively. From the kinetic analysis in batch fermentation, it was found that the specific cathepsin B inhibitor production rate (q$_{p}$) was linearly related to specific growh rate ($\mu$). The inhibitor in culture filtrate was purified by adsorption on activated charcoal, butanot extraction, silica gel chromatography, ion exchange chromatography using Dowex-1 (Cl form) and Amberlite IRC-50 (H$^{+}$ form), and preparative TLC. From the UV, IR, Mass spectroscopy and $^{1}$H-NMR, the inhibitor was thought to be a new inhibitor of which molecular weight was 199.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.10a
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• pp.351-353
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• 2018

The compensation characteristics are investigated as a function of the averaged residual dispersion per span (RDPS), which is different in each half transmission section, in dispersion-managed optical transmission links with a randomly distributed single mode fiber lengths and RDPS.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.10a
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• pp.348-350
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• 2018

In this paper, dispersion-managed optimal link configuration with the repetitively artificial-distributed single mode fiber lengths and residual dispersion per span, in which optical phase conjugator placed at midway, is proposed. It is confirmed that the proposed optical link configuration is suitable for expanding transmission length capable to obtain the good performance.